38 research outputs found
Infrared Observations of the Candidate LBV 1806-20 & Nearby Cluster Stars
We report near-infrared photometry, spectroscopy, and speckle imaging of the
hot, luminous star we identify as candidate LBV 1806-20. We also present
photometry and spectroscopy of 3 nearby stars, which are members of the same
star cluster containing LBV 1806-20 and SGR 1806-20. The spectroscopy and
photometry show that LBV 1806-20 is similar in many respects to the luminous
``Pistol Star'', albeit with some important differences. They also provide
estimates of the effective temperature and reddening of LBV 1806-20, and
confirm distance estimates, leading to a best estimate for the luminosity of
this star of . The nearby cluster stars have
spectral types and inferred absolute magnitudes which confirm the distance (and
thus luminosity) estimate for LBV 1806-20. If we drop kinematic measurements of
the distance ( kpc), we have a lower limit on the distance
of kpc, and on the luminosity of , based on
the cluster stars. If we drop both the kinematic and cluster star indicators
for distance, an ammonia absorption feature sets yet another lower limit to the
distance of kpc, with a corresponding luminosity estimate of for the candidate LBV 1806-20. Furthermore, based on very high
angular-resolution speckle images, we determine that LBV 1806-20 is not a
cluster of stars, but is rather a single star or binary system. Simple
arguments based on the Eddington luminosity lead to an estimate of the total
mass of LBV 1806-20 (single or binary) exceeding . We discuss
the possible uncertainties in these results, and their implications for the
star formation history of this cluster.Comment: 36 pages, including 8 figures (Figures 1 and 7 in JPG format due to
space); Accepted for publication in Ap
Development of a Multivalent Subunit Vaccine against Tularemia Using Tobacco Mosaic Virus (TMV) Based Delivery System
Francisella tularensisis a facultative intracellular pathogen, and is the causative agent of a fatal human disease known as tularemia. F. tularensis is classified as a Category A Biothreat agent by the CDC based on its use in bioweapon programs by several countries in the past and its potential to be used as an agent of bioterrorism. No licensed vaccine is currently available for prevention of tularemia. In this study, we used a novel approach for development of a multivalent subunit vaccine against tularemia by using an efficient tobacco mosaic virus (TMV) based delivery platform. The multivalent subunit vaccine was formulated to contain a combination of F. tularensis protective antigens: OmpA-like protein (OmpA), chaperone protein DnaK and lipoprotein Tul4 from the highly virulent F. tularensisSchuS4 strain. Two different vaccine formulations and immunization schedules were used. The immunized mice were challenged with lethal (10xLD100) doses of F. tularensisLVS on day 28 of the primary immunization and observed daily for morbidity and mortality. Results from this study demonstrate that TMV can be used as a carrier for effective delivery of multiple F. tularensisantigens. TMV-conjugate vaccine formulations are safe and multiple doses can be administered without causing any adverse reactions in immunized mice. Immunization with TMV-conjugated F. tularensisproteins induced a strong humoral immune response and protected mice against respiratory challenges with very high doses of F. tularensis LVS. This study provides a proof-of-concept that TMV can serve as a suitable platform for simultaneous delivery of multiple protective antigens of F. tularensis. Refinement of vaccine formulations coupled with TMV-targeting strategies developed in this study will provide a platform for development of an effective tularemia subunit vaccine as well as a vaccination approach that may broadly be applicable to many other bacterial pathogens
Photonic hydrogel sensors
Analyte-sensitive hydrogels that incorporate optical structures have emerged as sensing platforms for point-of-care diagnostics. The optical properties of the hydrogel sensors can be rationally designed and fabricated through self-assembly, microfabrication or laser writing. The advantages of photonic hydrogel sensors over conventional assay formats include label-free, quantitative, reusable, and continuous measurement capability that can be integrated with equipment-free text or image display. This Review explains the operation principles of photonic hydrogel sensors, presents syntheses of stimuli-responsive polymers, and provides an overview of qualitative and quantitative readout technologies. Applications in clinical samples are discussed, and potential future directions are identified
Synthesis and Characterization of N-Isopropylacrylamide Microspheres as pH Sensors
Swellable polymer microspheres that respond to pH were prepared by free radical dispersion polymerization using N-isopropylacrylamide (NIPA), N,N′-methylenebisacrylamide (MBA), 2,2-dimethoxy-2-phenylacetylphenone, N-tert-butylacrylamide (NTBA), and a pH-sensitive functional comonomer (acrylic acid, methacrylic acid, ethacrylic acid, or propacrylic acid). The diameter of the microspheres was between 0.5 and 1.0 μm. These microspheres were cast into hydrogel membranes prepared by mixing the pH-sensitive swellable polymer particles with aqueous polyvinyl alcohol (PVA) solutions followed by crosslinking with glutaric dialdehyde for use as pH sensors. Large changes in the turbidity of the PVA membrane were observed as the pH of the buffer solution in contact with the membrane was varied. These changes were monitored by UV–visible absorbance spectroscopy. Polymer swelling of many NIPA copolymers was reversible and independent of the ionic strength of the buffer solution in contact with the membrane. Both the degree of swelling and the apparent pKa of the polymer microspheres increased with temperature. Furthermore, the apparent pKa of the polymer particles could be tuned to respond sharply to pH in a broad range (pH 4.0–7.0) by varying the amount of crosslinker (MBA) and transition temperature modifier (NTBA), and the amount, pKa, and hydrophobicity of the pH-sensitive functional comonomer (alkyl acrylic acid) used in the formulation. Potential applications of these polymer particles include fiber optic pH sensing where the pH-sensitive material can be immobilized on the distol end of an optical fiber
Classification of the waxy condition of durum wheat by near infrared reflectance spectroscopy using wavelets and a genetic algorithm
Near infrared (NIR) reflectance spectroscopy has been applied to the problem of differentiating four genotypes of durum wheat: ‘waxy’, Wx A1 null null, wx-B1 null and wild type. The test data consisted of 95 NIR reflectance spectra of wheat samples obtained from a USDA-ARSwheat breeding program. A two-step procedure for pattern recognition analysis of NIR spectral data wasemployed. First, thewavelet packet transform [14,15] was applied to the NIR reflectance data usingwavelet filters at different scales to extract and separate low-frequency signal components from high frequency noise components. By applying these filters, each reflectance spectrum was decomposed into wavelet coefficients that represented the sample\u27s constituent frequencies. Second, wavelet coefficients characteristic of the waxy condition of the wheat samples were identified using a genetic algorithm for pattern recognition. The pattern recognition GA employed both supervised and unsupervised learning to identify wavelet coefficients that optimized clustering of the spectra by genotype in a plot of the two largest principal components of the data. By sampling key feature subsets, scoring their PC plots, and tracking those genotypes and samples that were difficult to classify, the pattern recognition GA was able to identify a set of wavelet coefficientswhose PC plot showed clustering of thewheat samples on the basis of their ‘waxy’ condition. Object validation was also performed to assess the predictive ability of the proposed NIR method to identify the ‘waxy’ condition of the wheat. An overall classification success rate of 78% was achieved for the spectral data
Chemometrics:Â From Basics to Wavelet Transform. Chemical Analysis, Volume 164 By Foo-Tim Chau (Hong Kong Polytechnic University), Yi-Zeng Liang (Central South University), Junbin Gao (University of New England), and Xue-Guang Shao (University of Science and Technology of China). John Wiley & Sons, Inc.:Â Hoboken, NJ. 2004. xiv + 316 pp. $99.95. ISBN 0-471-20242-8.
Evidential significance of automotive paint trace evidence using a pattern recognition based infrared library search engine for the Paint Data Query Forensic Database
Gluten Conformation at Different Temperatures and Additive Treatments
The effect of temperature (25, 45, and 65 °C) on the gluten secondary structure was investigated by using Fourier transform infrared (FTIR) spectroscopy and modulation of disulfide and hydrogen bonds contributions (100 ppm ascorbic acid (AA), 0.6% diacetyl tartaric acid ester of monoglycerides (DATEM), and 0.25 mM dithiothreitol (DTT)). The results showed that additives heated at 65 °C altered most of the gluten matrix formation by changing structural secondary structures compared to the secondary structures of native gluten (control). The content of random coils, α-helices, and β-sheet of gluten increased, while the extent of β-turns and antiparallel β-sheets decreased, which led to the transformation to a more stable secondary conformation. In addition, the rheological properties (%creep strain) revealed that gluten deformation increased during the heating process with all of the additives. The chemometric method could quantitate an overall alteration of gluten polymerization and gluten matrix formation during heating with additive treatments