La transmisión de la perspectiva histórica en la enseñanza de la psicología en el primer año de la carrera de Psicología de la UNLP

El presente trabajo se inscribe en el marco del proyecto de investigación La lectura y la escritura académicas en los primeros años de la carrera de Psicología de la UNLP. Su impacto en el aprendizaje de los estudiantes dirigido por la Dra. Ana María Talak en la Facultad de Psicología de la Universidad Nacional de La Plata cuyos objetivos consisten, por un lado, en conocer las características socio-demográficas de la población de estudiantes de los dos primeros años de la carrera y, por otro, en relevar evidencia acerca del impacto en el aprendizaje de las estrategias pedagógicas implementadas en las asignaturas Psicología I y Psicología II que busquen fortalecer las habilidades de lectura y de escritura académicas. El objetivo de este trabajo consiste en describir las implicancias de la adopción de la perspectiva histórica en la enseñanza de la psicología, objetivo general de la asignatura Psicología I, y asimismo presentar la propuesta metodológica de enseñanza y evaluación que se implementa al finalizar la cursada.Eje temático: Enseñanza de la psicologíaFacultad de Psicologí

Evaluación del impacto de la enseñanza de la perspectiva histórica en los alumnos de primer año de la carrera de Psicología de la UNLP

El presente trabajo se inscribe en el marco del proyecto de investigación de las cátedras Psicología I y II, La lectura y la escritura académicas en los primeros años de la carrera de Psicología de la Universidad Nacional de La Plata. Su impacto en el aprendizaje de los estudiantes dirigido por la Dra. Ana María Talak en la Facultad de Psicología de la UNLP. Se focaliza en la propuesta de la asignatura Psicología I. La misma tiene como objetivo indagar los desarrollos de la psicología durante los siglos XIX y XX desde una perspectiva histórico- epistemológica para el abordaje de las distintas tradiciones de investigación, las prácticas profesionales y los discursos teniendo en cuenta su implantación cultural. De esta manera, se busca promover en los alumnos el desenvolvimiento de herramientas metodológicas y conceptuales a partir del estudio histórico-crítico de temas específicos, intentando mostrar que las teorías psicológicas son respuestas a problemas planteados en contextos históricos.Eje temático: Enseñanza de la psicologíaFacultad de Psicologí

Evaluación del impacto de la enseñanza de la perspectiva histórica en los alumnos de primer año de la carrera de Psicología de la UNLP

El presente trabajo se inscribe en el marco del proyecto de investigación de las cátedras Psicología I y II, La lectura y la escritura académicas en los primeros años de la carrera de Psicología de la Universidad Nacional de La Plata. Su impacto en el aprendizaje de los estudiantes dirigido por la Dra. Ana María Talak en la Facultad de Psicología de la UNLP. Se focaliza en la propuesta de la asignatura Psicología I. La misma tiene como objetivo indagar los desarrollos de la psicología durante los siglos XIX y XX desde una perspectiva histórico- epistemológica para el abordaje de las distintas tradiciones de investigación, las prácticas profesionales y los discursos teniendo en cuenta su implantación cultural. De esta manera, se busca promover en los alumnos el desenvolvimiento de herramientas metodológicas y conceptuales a partir del estudio histórico-crítico de temas específicos, intentando mostrar que las teorías psicológicas son respuestas a problemas planteados en contextos históricos.Eje temático: Enseñanza de la psicologíaFacultad de Psicologí

La transmisión de la perspectiva histórica en la enseñanza de la psicología en el primer año de la carrera de Psicología de la UNLP

El presente trabajo se inscribe en el marco del proyecto de investigación La lectura y la escritura académicas en los primeros años de la carrera de Psicología de la UNLP. Su impacto en el aprendizaje de los estudiantes dirigido por la Dra. Ana María Talak en la Facultad de Psicología de la Universidad Nacional de La Plata cuyos objetivos consisten, por un lado, en conocer las características socio-demográficas de la población de estudiantes de los dos primeros años de la carrera y, por otro, en relevar evidencia acerca del impacto en el aprendizaje de las estrategias pedagógicas implementadas en las asignaturas Psicología I y Psicología II que busquen fortalecer las habilidades de lectura y de escritura académicas. El objetivo de este trabajo consiste en describir las implicancias de la adopción de la perspectiva histórica en la enseñanza de la psicología, objetivo general de la asignatura Psicología I, y asimismo presentar la propuesta metodológica de enseñanza y evaluación que se implementa al finalizar la cursada.Eje temático: Enseñanza de la psicologíaFacultad de Psicologí

Identification of PVR (CD155) and Nectin-2 (CD112) as Cell Surface Ligands for the Human DNAM-1 (CD226) Activating Molecule

Human natural killer (NK) cells express a series of activating receptors and coreceptors that are involved in recognition and killing of target cells. In this study, in an attempt to identify the cellular ligands for such triggering surface molecules, mice were immunized with NK-susceptible target cells. On the basis of a functional screening, four mAbs were selected that induced a partial down-regulation of the NK-mediated cytotoxicity against the immunizing target cells. As revealed by biochemical analysis, three of such mAbs recognized molecules of ∼70 kD. The other mAb reacted with two distinct molecules of ∼65 and 60 kD, respectively. Protein purification followed by tryptic digestion and mass spectra analysis, allowed the identification of the 70 kD and the 65/60 kD molecules as PVR (CD155) and Nectin-2 δ/α (CD112), respectively. PVR-Fc and Nectin-2-Fc soluble hybrid molecules brightly stained COS-7 cells transfected with the DNAM-1 (CD226) construct, thus providing direct evidence that both PVR and Nectin-2 represent specific ligands for the DNAM-1 triggering receptor. Finally, the surface expression of PVR or Nectin-2 in cell transfectants resulted in DNAM-1–dependent enhancement of NK-mediated lysis of these target cells. This lysis was inhibited or even virtually abrogated upon mAb-mediated masking of DNAM-1 (on NK cells) or PVR or Nectin-2 ligands (on cell transfectants)

LIOFeron®TB/LTBI: A novel and reliable test for LTBI and tuberculosis

Objectives: High accuracy diagnostic screening tests for tuberculosis (TB) are required to improve the diagnosis of both active TB and latent Mycobacterium tuberculosis (MTB) infection (LTBI). The novel IGRA LIOFeron®TB/LTBI assay was tested and its accuracy was compared to the QuantiFERON®-TB Gold Plus assay. Methods: A total of 389 subjects were enrolled in two cohorts and classified as healthy, active TB or LTBI persons. The blood of all the patients was tested with LIOFeron®TB/LTBI assay, containing MTB alanine dehydrogenase, able to differentiate active TB from LTBI diagnosis. The results obtained with both IGRAs, performed on the same 250 samples, were finally compared. Results: The two assays demonstrated an excellent concordance of their results with patients' diagnosis of MTB infection. ROC analysis for QuantiFERON®-TB Gold Plus showed sensitivity and specificity respectively of 98% and 97% in diagnosing active TB patients and 85% and 94% in diagnosing LTBI subjects. LIOFeron®TB/LTBI assay showed sensitivity and specificity respectively of 90% and 98% in diagnosing active TB patients and 94% and 97% in diagnosing LTBI subjects. Conclusions: The two IGRAs displayed the same high accuracy in diagnosing MTB infection/TB disease, and LIOFeron®TB/LTBI assay demonstrated higher sensitivity than QuantiFERON®-TB Gold Plus test in LTBI detection. Keywords: Mycobacterium tuberculosis, Tuberculosis diagnosis, IGRA, Alanine dehydrogenas

Sphingolipid dysregulation due to lack of functional KDSR impairs proplatelet formation causing thrombocytopenia.

Sphingolipids are fundamental to membrane trafficking, apoptosis, and cell differentiation and proliferation. KDSR or 3-keto-dihydrosphingosine reductase is an essential enzyme for de novo sphingolipid synthesis, and pathogenic mutations in KDSR result in the severe skin disorder erythrokeratodermia variabilis et progressiva-4 Four of the eight reported cases also had thrombocytopenia but the underlying mechanism has remained unexplored. Here we expand upon the phenotypic spectrum of KDSR deficiency with studies in two siblings with novel compound heterozygous variants associated with thrombocytopenia, anemia, and minimal skin involvement. We report a novel phenotype of progressive juvenile myelofibrosis in the propositus, with spontaneous recovery of anemia and thrombocytopenia in the first decade of life. Examination of bone marrow biopsies showed megakaryocyte hyperproliferation and dysplasia. Megakaryocytes obtained by culture of CD34+ stem cells confirmed hyperproliferation and showed reduced proplatelet formation. The effect of KDSR insufficiency on the sphingolipid profile was unknown, and was explored in vivo and in vitro by a broad metabolomics screen that indicated activation of an in vivo compensatory pathway that leads to normalization of downstream metabolites such as ceramide. Differentiation of propositus-derived induced pluripotent stem cells to megakaryocytes followed by expression of functional KDSR showed correction of the aberrant cellular and biochemical phenotypes, corroborating the critical role of KDSR in proplatelet formation. Finally, Kdsr depletion in zebrafish recapitulated the thrombocytopenia and showed biochemical changes similar to those observed in the affected siblings. These studies support an important role for sphingolipids as regulators of cytoskeletal organization during megakaryopoiesis and proplatelet formation

Transcriptional profiling of the human fibrillin/LTBP gene family, key regulators of mesenchymal cell functions

The fibrillins and latent transforming growth factor binding proteins (LTBPs) form a superfamily of extracellular matrix (ECM) proteins characterized by the presence of a unique domain, the 8-cysteine transforming growth factor beta (TGFβ) binding domain. These proteins are involved in the structure of the extracellular matrix and controlling the bioavailability of TGFβ family members. Genes encoding these proteins show differential expression in mesenchymal cell types which synthesize the extracellular matrix. We have investigated the promoter regions of the seven gene family members using the FANTOM5 CAGE database for human. While the protein and nucleotide sequences show considerable sequence similarity, the promoter regions were quite diverse. Most genes had a single predominant transcription start site region but LTBP1 and LTBP4 had two regions initiating different transcripts. Most of the family members were expressed in a range of mesenchymal and other cell types, often associated with use of alternative promoters or transcription start sites within a promoter in different cell types. FBN3 was the lowest expressed gene, and was found only in embryonic and fetal tissues. The different promoters for one gene were more similar to each other in expression than to promoters of the other family members. Notably expression of all 22 LTBP2 promoters was tightly correlated and quite distinct from all other family members. We located candidate enhancer regions likely to be involved in expression of the genes. Each gene was associated with a unique subset of transcription factors across multiple promoters although several motifs including MAZ, SP1, GTF2I and KLF4 showed overrepresentation across the gene family. FBN1 and FBN2, which had similar expression patterns, were regulated by different transcription factors. This study highlights the role of alternative transcription start sites in regulating the tissue specificity of closely related genes and suggests that this important class of extracellular matrix proteins is subject to subtle regulatory variations that explain the differential roles of members of this gene family

Differential cross section measurements for the production of a W boson in association with jets in proton–proton collisions at √s = 7 TeV

Measurements are reported of differential cross sections for the production of a W boson, which decays into a muon and a neutrino, in association with jets, as a function of several variables, including the transverse momenta (pT) and pseudorapidities of the four leading jets, the scalar sum of jet transverse momenta (HT), and the difference in azimuthal angle between the directions of each jet and the muon. The data sample of pp collisions at a centre-of-mass energy of 7 TeV was collected with the CMS detector at the LHC and corresponds to an integrated luminosity of 5.0 fb[superscript −1]. The measured cross sections are compared to predictions from Monte Carlo generators, MadGraph + pythia and sherpa, and to next-to-leading-order calculations from BlackHat + sherpa. The differential cross sections are found to be in agreement with the predictions, apart from the pT distributions of the leading jets at high pT values, the distributions of the HT at high-HT and low jet multiplicity, and the distribution of the difference in azimuthal angle between the leading jet and the muon at low values.United States. Dept. of EnergyNational Science Foundation (U.S.)Alfred P. Sloan Foundatio