EWS/ETS Regulates the Expression of the Dickkopf Family in Ewing Family Tumor Cells

BACKGROUND: The Dickkopf (DKK) family comprises a set of proteins that function as regulators of Wnt/beta-catenin signaling and has a crucial role in development. Recent studies have revealed the involvement of this family in tumorigenesis, however their role in tumorigenesis is still remained unclear. METHODOLOGY/PRINCIPAL FINDINGS: We found increased expression of DKK2 but decreased expression of DKK1 in Ewing family tumor (EFT) cells. We showed that EFT-specific EWS/ETS fusion proteins enhance the DKK2 promoter activity, but not DKK1 promoter activity, via ets binding sites (EBSs) in the 5' upstream region. EWS/ETS-mediated transactivation of the promoter was suppressed by the deletion and mutation of EBSs located upstream of the DKK2 gene. Interestingly, the inducible expression of EWS/ETS resulted in the strong induction of DKK2 expression and inhibition of DKK1 expression in human primary mesenchymal progenitor cells that are thought to be a candidate of cell origin of EFT. In addition, using an EFT cell line SK-ES1 cells, we also demonstrated that the expression of DKK1 and DKK2 is mutually exclusive, and the ectopic expression of DKK1, but not DKK2, resulted in the suppression of tumor growth in immuno-deficient mice. CONCLUSIONS/SIGNIFICANCE: Our results suggested that DKK2 could not functionally substitute for DKK1 tumor-suppressive effect in EFT. Given the mutually exclusive expression of DKK1 and DKK2, EWS/ETS regulates the transcription of the DKK family, and the EWS/ETS-mediated DKK2 up-regulation could affect the tumorigenicity of EFT in an indirect manner

Evolutionary Map of the Universe (EMU): 18-cm OH-maser discovery in ASKAP continuum images of the SCORPIO field

Abstract Low- and intermediate-mass stars end their life dispersing their outer layers into the circumstellar medium, during the asymptotic and post-asymptotic giant branch phases. OH masers at 18 cm offer an effective way to probe their circumstellar environment. In this work we present the discovery of seven OH maser sources likely associated with such evolved stars from the visual inspection of ASKAP continuum images. These seven sources do not emit real continuum emission, but the high sensitivity of our images allows us to detect their maser emission, resembling continuum sources. To confirm their nature, we carried out spectral-line observations with ATCA. All the sources showed the double-peaked spectra at 1612 MHz, typical of evolved stars. The detection of maser emission in continuum images can be a complementary and easy-to-use method to discover new maser sources with the large-area deep surveys conducted with the SKA precursors. The implication for radio stars studies are remarkable since pure OH maser sources (i.e. with no continuum associated) represent, at a sensitivity of $100\ \mu \mathrm{Jy\ beam}^{-1}$, about 4 percent of all Galactic sources and by far the most numerous stellar population.</jats:p

Two-pion Bose-Einstein correlations in central Pb-Pb collisions at sNN\sqrt{s_{\rm NN}} = 2.76 TeV

The first measurement of two-pion Bose-Einstein correlations in central Pb-Pb collisions at $\sqrt{s_{\rm NN}} = 2.76$ TeV at the Large Hadron Collider is presented. We observe a growing trend with energy now not only for the longitudinal and the outward but also for the sideward pion source radius. The pion homogeneity volume and the decoupling time are significantly larger than those measured at RHIC.Comment: 17 pages, 5 captioned figures, 1 table, authors from page 12, published version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/388

Suppression of charged particle production at large transverse momentum in central Pb-Pb collisions at sNN=2.76\sqrt{s_{\rm NN}} = 2.76 TeV

Inclusive transverse momentum spectra of primary charged particles in Pb-Pb collisions at $\sqrt{s_{_{\rm NN}}}$ = 2.76 TeV have been measured by the ALICE Collaboration at the LHC. The data are presented for central and peripheral collisions, corresponding to 0-5% and 70-80% of the hadronic Pb-Pb cross section. The measured charged particle spectra in $|\eta|<0.8$ and $0.3 < p_T < 20$ GeV/$c$ are compared to the expectation in pp collisions at the same $\sqrt{s_{\rm NN}}$, scaled by the number of underlying nucleon-nucleon collisions. The comparison is expressed in terms of the nuclear modification factor $R_{\rm AA}$. The result indicates only weak medium effects ($R_{\rm AA} \approx$ 0.7) in peripheral collisions. In central collisions, $R_{\rm AA}$ reaches a minimum of about 0.14 at $p_{\rm T}=6$-7GeV/$c$ and increases significantly at larger $p_{\rm T}$. The measured suppression of high-$p_{\rm T}$ particles is stronger than that observed at lower collision energies, indicating that a very dense medium is formed in central Pb-Pb collisions at the LHC.Comment: 15 pages, 5 captioned figures, 3 tables, authors from page 10, published version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/98

Effective Rheology of Bubbles Moving in a Capillary Tube

We calculate the average volumetric flux versus pressure drop of bubbles moving in a single capillary tube with varying diameter, finding a square-root relation from mapping the flow equations onto that of a driven overdamped pendulum. The calculation is based on a derivation of the equation of motion of a bubble train from considering the capillary forces and the entropy production associated with the viscous flow. We also calculate the configurational probability of the positions of the bubbles.Comment: 4 pages, 1 figur

A first glimpse at the Galactic plane with the ASKAP: the SCORPIO field

In the broad context of the Australian Square Kilometer Array Pathfinder (ASKAP) early-science phase and preparation for the related surveys, we report the first radio observations towards the Galactic plane. The targeted field was chosen to encompass the entire SCORPIO survey, one of the several pathfinder projects for the Evolutionary Map of the Universe survey planned with the ASKAP. The observations were carried out in 2018 January at a central frequency of 912 MHz, with 15 operational antennas, and covered a total area of about 40 square degrees in three different pointings. The final image has a resolution of 24.1 × 21.1 arcsec2 and a median rms of 541 μJy beam−1⁠. We were able to extract 3545 candidate sources, 75 per cent of them point sources. For a preliminary validation, a comparison with the 843 MHz Molonglo Galactic Plane Survey is presented. Although the present observations were obtained with the ASKAP only partially deployed, its unique capability to map complex sources, such as those inhabiting the Galactic plane, at different angular scales, is highlighted. Within the SCORPIO field all the previously classified H II regions, Planetary Nebulae (PNe), and supernovae remnants (SNRs), previously known to be radio sources, were detected. We also report new radio detections from several H II regions previously classified as ‘candidates’ or ‘radio quiet’ and from half of all the PNe in the SCORPIO field with robust classification. Most notably, we find numerous unclassified, extended sources which constitute a promising sample of candidates H II regions and SNRs

Alignment of the ALICE Inner Tracking System with cosmic-ray tracks

37 pages, 15 figures, revised version, accepted by JINSTALICE (A Large Ion Collider Experiment) is the LHC (Large Hadron Collider) experiment devoted to investigating the strongly interacting matter created in nucleus-nucleus collisions at the LHC energies. The ALICE ITS, Inner Tracking System, consists of six cylindrical layers of silicon detectors with three different technologies; in the outward direction: two layers of pixel detectors, two layers each of drift, and strip detectors. The number of parameters to be determined in the spatial alignment of the 2198 sensor modules of the ITS is about 13,000. The target alignment precision is well below 10 micron in some cases (pixels). The sources of alignment information include survey measurements, and the reconstructed tracks from cosmic rays and from proton-proton collisions. The main track-based alignment method uses the Millepede global approach. An iterative local method was developed and used as well. We present the results obtained for the ITS alignment using about 10^5 charged tracks from cosmic rays that have been collected during summer 2008, with the ALICE solenoidal magnet switched off.Peer reviewe

First measurement of the |t|-dependence of coherent J/ψ photonuclear production

The first measurement of the cross section for coherent J/ψ photoproduction as a function of |t|, the square of the momentum transferred between the incoming and outgoing target nucleus, is presented. The data were measured with the ALICE detector in ultra-peripheral Pb–Pb collisions at a centre-of-mass energy per nucleon pair sNN=5.02TeV with the J/ψ produced in the central rapidity region |y|<0.8, which corresponds to the small Bjorken-x range (0.3−1.4)×10−3. The measured |t|-dependence is not described by computations based only on the Pb nuclear form factor, while the photonuclear cross section is better reproduced by models including shadowing according to the leading-twist approximation, or gluon-saturation effects from the impact-parameter dependent Balitsky–Kovchegov equation. These new results are therefore a valid tool to constrain the relevant model parameters and to investigate the transverse gluonic structure at very low Bjorken-x.publishedVersio

Radio continuum sources behind the Large Magellanic Cloud

We present a comprehensive multifrequency catalogue of radio sources behind the Large Magellanic Cloud (LMC) between 0.2 and 20 GHz, gathered from a combination of new and legacy radio continuum surveys. This catalogue covers an area of ∼144 deg2 at angular resolutions from 45 arcsec to ∼3 arcmin. We find 6434 discrete radio sources in total, of which 3789 are detected at two or more radio frequencies. We estimate the median spectral index (α; where Sv ∼ να) of α = −0.89 and mean of −0.88 ± 0.48 for 3636 sources detected exclusively at two frequencies (0.843 and 1.384 GHz) with similar resolution [full width at half-maximum (FWHM) ∼40–45 arcsec]. The large frequency range of the surveys makes it an effective tool to investigate Gigahertz Peak Spectrum (GPS), Compact Steep Spectrum (CSS), and Infrared Faint Radio Source (IFRS) populations within our sample. We find 10 GPS candidates with peak frequencies near 5 GHz, from which we estimate their linear size. 1866 sources from our catalogue are CSS candidates with α  &amp;lt; −0.8. We found six candidates for High Frequency Peaker (HFP) sources, whose radio fluxes peak above 5 GHz and no sources with unconstrained peaks and α  &amp;gt; 0.5. We found optical counterparts for 343 of the radio continuum sources, of which 128 have a redshift measurement. Finally, we investigate the population of 123 IFRSs found in this study

Resolving the strange behavior of extraterrestrial potassium in the upper atmosphere

It has been known since the 1960s that the layers of Na and K atoms, which occur between 80 and 105 km in the Earth's atmosphere as a result of meteoric ablation, exhibit completely different seasonal behavior. In the extratropics Na varies annually, with a pronounced wintertime maximum and summertime minimum. However, K varies semiannually with a small summertime maximum and minima at the equinoxes. This contrasting behavior has never been satisfactorily explained. Here we use a combination of electronic structure and chemical kinetic rate theory to determine two key differences in the chemistries of K and Na. First, the neutralization of K+ ions is only favored at low temperatures during summer. Second, cycling between K and its major neutral reservoir KHCO3 is essentially temperature independent. A whole atmosphere model incorporating this new chemistry, together with a meteor input function, now correctly predicts the seasonal behavior of the K layer