Plan de negocios para el Laboratorio de Biotecnología de Plantas y el Laboratorio de Fisiología Vegetal del TEC Campus Tecnológico Local San Carlos

Trabajo Final de Graduación (Bachillerato en Administración de Empresas). Instituto Tecnológico de Costa Rica, Escuela de Administración de Empresas, 2018.This document shows a Business Plan for the Plant Biotechnology Laboratory and the Plant Physiology Laboratory of Technological of Costa Rica, San Carlos Local Technological Campus. To carry out the study, the research was through a mixed-type approach with descriptive scope, on the sector in which the laboratories works, analyzing the market information and numerical calculations were made to determine the operating costs of the laboratories, getting a financial plan with projections of cash flows that reflect the potential in its commercial functions. The realization of these plans is important for a competitive and strategic development of the laboratories. It presents aspects that strengthen and contribute to key activities such as strategic planning and marketing. In addition, these plans provide the necessary organizational and technical components to quantify in an accurate way the costs that both laboratories will incur in the sale of their services.Campus Tecnológico Local San Carlo

Systemic lymphoma masquerading as Vogt-Koyanagi-Harada syndrome: Report of a case with multimodal imaging and histopathology.

PurposeTo report a case of systemic diffuse large B cell lymphoma presenting with ocular manifestations and neurologic findings resembling Vogt-Koyanagi-Harada syndrome.ObservationsA 51-year-old Caucasian man presented with headache, ear pain, and blurry vision in both eyes. He was found to have bilateral exudative retinal detachments. After a short period of initial improvement with high dose systemic corticosteroid, his condition significantly worsened. An extensive work-up, including a kidney biopsy, led to a diagnosis of systemic diffuse large B cell lymphoma. He had excellent recovery following treatment with appropriate chemotherapy.Conclusions and importanceSystemic malignancy may present with ocular manifestations and may masquerade as another diagnosis. An unexpected clinical course may suggest an alternative diagnosis. A broad systemic work-up including an evaluation for malignancy should be considered for patients presenting with unexplained exam or systemic findings

Subretinal mononuclear cells in Coats\u27 Disease studied with RPE65 and CD163: Evidence for histiocytoid pigment epithelial cells

PURPOSE: To evaluate the mononuclear cells in the subretinal exudate in Coats\u27 disease. DESIGN: Retrospective case series. METHODS: Five enucleated globes and 1 cytology sample from a patient with Coats\u27 disease and 1 case of chronic retinal detachment following repair of an open globe injury were examined immunohistochemically to identify intraretinal and subretinal exudative cells. The 2 biomarkers were RPE65 for retinal pigment epithelium and CD163 for histiocytes, each tagged with different chromogens, yellow for pigment epithelium and purple for CD163-positive (CD163) monocytes/histiocytes. Expression levels were sought from both biomarkers together and singly. A color shift to red in the cells\u27 chromogenic reaction indicated the simultaneous presence of the 2 biomarkers. RESULTS: Most of the mononuclear cells in Coats\u27 disease samples were CD163 (purple), and a minority were RPE65 (yellow). An intermediate number of cells were RPE65/CD163 (orange-red). The eye with a chronic retinal detachment had an equal distribution of CD163 and RPE65/CD163 cells. CONCLUSIONS: RPE has several well-delineated phenotypes and functions. In normal visual physiology, the pigment epithelium supports photoreceptors and participates in their renewal by phagocytosis of the tips of the photoreceptors. The expression of CD163, a feature of hematopoietically derived monocytes, together with RPE65 in the retinal pigment epithelium, supports differentiation toward histiocytes. Yellow staining of detached pigment epithelial cells were rare. The presence of histiocytoid pigment epithelium at the Bruch membrane probably also has implications for macular degeneration

Recurrent keratoconus: an analysis of breaks in Bowman’s layer in corneal grafts

Objective To study in a masked fashion whether an objective histological feature associated with keratoconus (KCN) occurs in donor corneas in eyes originally receiving a corneal graft for KCN.Methods Two ocular pathologists performed a retrospective masked histological analysis of slides from donor buttons recovered from 21 eyes with a history of KCN undergoing repeat penetrating keratoplasty (failed-PK-KCN), 11 eyes that underwent their first PK due to KCN (primary KCN), and 11 eyes without history of KCN which underwent PK for other conditions (failed-PK-non-KCN). Breaks/gaps in Bowman’s layer served as the pathological feature indicative of recurrent KCN.Results Breaks in Bowman’s layer were present in 18/21 (86%) of the failed-PK-KCN group, 10/11 (91%) of the primary KCN group, and in 3/11 (27%) of the failed-PK-non-KCN group. Pathological evidence suggests that the prevalence of breaks is significantly higher in grafted patients with a history of KCN than non-KCN controls (OR: 16.0, 95% CI 2.63 to 97.2, Fisher’s exact test p=0.0018) with a conservative Bonferroni criterion of p <0.017 to account for multiple group comparisons. There was no statistically significant difference found between the failed-PK-KCN and primary KCN groups.Conclusions This study provides histological evidence that breaks and gaps in Bowman’s layer, consistent with those found in primary KCN, may develop within the donor tissue in eyes with a history of KCN

Bocetos - AR168 - 202102

Descripción: Curso electivo de especialidad en la carrera de Arquitectura y curso de la mención en Expresión Gráfica, de carácter teórico-práctico dirigido a los estudiantes de sexto ciclo. El curso de Bocetos es un curso electivo de la carrera de Arquitectura, el cual consiste en dar a los estudiantes herramientas que le permitan expresar, de manera sintética, dibujos donde pueda comunicar las características propias de un objeto, un lugar, un edificio. Se suma a esto la necesidad de realizarlo en poco tiempo y de forma clara, destacando los aspectos más importantes. Propósito: La presente asignatura tiene como propósito que el estudiante desarrolle herramientas que le permitan comprender, analizar y estructurar una representación gráfica tridimensional en un tiempo determinado. Desarrollando la competencia específica de la carrera, Técnica y construcción (que corresponde al criterio NAAB SC1 en Nivel 2). Tiene como requisito el curso AR351 Dibujo Arquitectónic