9 research outputs found
Gene Expression Profile of HDF in SMG Partially Overlaps with That in the NASA Twins Study
Microgravity research is an important field in biomedical sciences not only due to our interest in exploring and living in space, but also because of the insights it gives on earthbound health conditions. Using a human dermal fibroblast (HDF) cell line cultured in simulated microgravity (SMG) in combination with high throughput cDNA microarrays and quantitative Northern analysis, 271 differentially regulated genes were identified and 72% of these genes were also reported in the high throughput gene expression data of the recent National Aeronautics and Space Administration (NASA) Twins Study. The identification of the large number of overlapping microgravity sensitive genes between the skin fibroblast in microgravity and astronautâs peripheral blood mononuclear cells (PBMCs) indicated that microgravity alone, without space radiation, was able to elicit an adaptive response involving a set of about 200 genes. Further analysis of the overlapping genes with the same direction of regulation (86 genes) and opposite direction of regulation (108 genes) revealed important pathways and cellular processes in the microgravity adaptation responses
Gene Expression Microarrays in Microgravity Research: Toward the Identification of Major Space Genes
Serine Phosphorylation of SR Proteins Is Required for Their Recruitment to Sites of Transcription In Vivo
Expression of most RNA polymerase II transcripts requires the coordinated execution of transcription, splicing, and 3âČ processing. We have previously shown that upon transcriptional activation of a gene in vivo, pre-mRNA splicing factors are recruited from nuclear speckles, in which they are concentrated, to sites of transcription (Misteli, T., J.F. CĂĄceres, and D.L. Spector. 1997. Nature. 387:523â527). This recruitment process appears to spatially coordinate transcription and pre-mRNA splicing within the cell nucleus. Here we have investigated the molecular basis for recruitment by analyzing the recruitment properties of mutant splicing factors. We show that multiple protein domains are required for efficient recruitment of SR proteins from nuclear speckles to nascent RNA. The two types of modular domains found in the splicing factor SF2/ ASF exert distinct functions in this process. In living cells, the RS domain functions in the dissociation of the protein from speckles, and phosphorylation of serine residues in the RS domain is a prerequisite for this event. The RNA binding domains play a role in the association of splicing factors with the target RNA. These observations identify a novel in vivo role for the RS domain of SR proteins and suggest a model in which protein phosphorylation is instrumental for the recruitment of these proteins to active sites of transcription in vivo
Rapid induction of nuclear transcripts and inhibition of intron decay in response to the polymerase II inhibitor DRB
Characterization of the Mel1c melatoninergic receptor in platypus (Ornithorhynchus anatinus)
Measurement of event shapes at large momentum transfer with the ATLAS detector in collisions at = 7 TeV
A measurement of event shape variables is presented for large momentum
transfer proton-proton collisions using the ATLAS detector at the Large Hadron
Collider. Six event shape variables calculated using hadronic jets are studied
in inclusive multi-jet events in 35 pb^-1 of integrated luminosity at a
center-of-mass energy of sqrt(s) = 7 TeV. These measurements are compared to
predictions by three Monte Carlo event generators containing
leading-logarithmic parton showers matched to leading order matrix elements for
2 to 2 and 2 to n (n=2,...6) scattering. Measurements of the third-jet
resolution parameter, aplanarity, thrust, sphericity, and transverse sphericity
are generally well described. The mean value of each event shape variable is
evaluated as a function of the average momentum of the two leading jets pT1 and
pT2, with a mean pT approaching 1 TeV.Comment: 11 pages plus author list (25 pages total), 3 figures, submitted to
European Physical Journal