In Vitro Activity Of Artemisia Annua L (asteraceae) Extracts Against Rhipicephalus (boophilus) Microplus [atividade In Vitro De Extratos De Artemisia Annua L (asteraceae) Sobre Rhipicephalus (boophilus) Microplus]

The activity of plant extracts on parasites may indicate groups of substances that are potentially useful for controlling Rhipicephalus (Boophilus) microplus. The aim of the present study was to investigate the in vitro action of Artemisia annua extracts on this tick. The concentrations of the sesquiterpene lactones artemisinin and deoxyartemisinin present in plant extracts were quantified via high-performance liquid chromatography. Four extracts produced from the concentrated crude extract (CCE) were evaluated on larvae using the impregnated paper method, with readings after 24 hours of incubation. The engorged females were immersed in the CCE and in its four derived extracts for five minutes, with incubation for subsequent analysis of biological parameters. The extracts were not effective on the larvae at the concentrations tested (3.1 to 50 mg.mL-1). The CCE showed greater efficacy on engorged females (EC50 of 130.6 mg.mL-1 and EC90 of 302.9 mg.mL-1) than did the derived extracts. These results tend to confirm that the action of artemisinin on engorged females of R. (B.) microplus is conditional to their blood intake. In this case, in vitro methods would be inadequate for effective evaluation of the action of A. annua on R. (B.) microplus.2013135Amaral, N.K., Guidelines for the evaluation of ixodicides against the cattle tick Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) (1993) Revista Brasileira De Parasitologia Veterinária, 2 (2), pp. 144-151(2009) Anuário Da Pecuária Brasileira, p. 360. , ANUALPEC, Sao Paulo: Angra FNP PesquisasBorges, L.M.F., Acao do extrato hexanico de frutos maduros de Melia azedarach (Meliaceae) sobre Boophilus microplus (Acari: Ixodidae) em bezerros infestados artificialmente (2005) Revista De Patologia Tropical, 34 (1), pp. 53-59Cala, A.C., (2010) Avaliação Da Atividade De Artemisia Annua L., Melia Azedarach L. E Trichilia Claussenii C. Sobre Nematódeos Gastrintestinais De Ovinos, p. 64. , Dissertacao (Mestrado)- Universidade Estadual Paulista, Faculdade de Ciencias Agrarias e Veterinarias, JaboticabalCeleghini, R.M.S., Desenvolvimento e validacao de metodologia analitica por CLAE-IR para determinacao de artemisinina em Artemisia annua L (2009) Química Nova, 32 (4), pp. 875-878Chagas, A.C.S., Efeito acaricida de oleos essenciais e concentrados emulsionaveis de Eucalyptus em Boophilus microplus (2002) Brazilian Journal of Veterinary Research and Animal Science, 39 (5), pp. 247-253Chagas, A.C.S., Sensibilidade do carrapato Boophilus microplus a solventes (2003) Ciência Rural, 33 (1), pp. 109-114Creek, D.J., Kinetics of ironmediated artemisinin degradation:Effect of solvent composition and iron salt (2005) Journal of Pharmaceutical Sciences, 94 (8), pp. 1820-1829Drummond, R.O., Boophilus annulatus and B. Microplus: Laboratory tests of insecticides (1973) Journal of Economic Entomology, 66 (1), pp. 130-133Ekanem, A.P., Brisibe, E.A., Effects of ethanol extract of Artemisia annua L. Against monogenean parasites of Heterobranchus longifilis (2010) Parasitology Research, 106 (5), pp. 1135-1139Fernandes, F.F., Freitas, E.P.S., Acaricidal activity of an oleoresinous extract from Copaifera reticulata (Leguminosae: Caesalpinioideae against larvae of the southern cattle tick, Riphicephalus (Boophilus) microplus (Acari:Ixodidae) (2007) Veterinary Parasitology, 147 (1-2), pp. 150-154Ferreira, J.F.S., Agrotechnological aspects of the anti-malarial plant Artemisia annua and its potential use in animal health in Appalachia (2006) International Symposium on Perfume, Aromatic and Medicinal Plants, (2-4), pp. 797-804. , Revue de Regions Arides - Numero special - Actes du seminaire international les Plants a Parfum, Aromatiques et Medicinales (English version: International Center for Agricultural Research in Dry AreasFerreira, J.F.S., Nutrient Deficiency in the Production of Artemisinin, Dihydroartemisinic Acid, and Artemisinic Acid in Artemisia annua L (2007) Journal of Agricultural and Food Chemistry, 55 (5), pp. 1686-1694Ferreira, J.F.S., Gonzalez, J.M., Chemical and biological stability of artemisinin in bovine rumen fluid and its kinetics in goats (Capra hircus) (2008) Revista Brasileira De Parasitologia Veterinária, 17 (SUPPL. 1), pp. 103-109Furlong, J., O carrapato dos bovinos e a resistencia: Temos o que comemorar? (2007) A Hora Veterinária, 159, pp. 26-32Grisi, L., Impacto economico das principais ectoparasitoses em bovinos no Brasil (2002) A Hora Veterinária, 21 (125), pp. 8-10Heimerdinger, A., Extrato alcoolico de Capim-cidreira (Cymbopogon citratus) no controle do Boophilus microplus em bovinos (2006) Revista Brasileira De Parasitologia Veterinária, 15 (1), pp. 37-39Idris, U.A., Adam, S.E., Tartour, G., The anthelmintic efficacy of Artemisia herba-alba against Haemonchus contortus infection in goats (1982) Animal Health Quarterly, 22 (3), pp. 138-143Iqbal, Z., Anthelmintic activity of Artemisia brevifolia in sheep (2004) Journal of Ethnopharmacology, 93 (2), pp. 265-268Keiser, J., Artesunate and artemether are effective fasciolicides in the rat model and in vitro (2006) Journal of Antimicrobial Chemotherapy, 57 (6), pp. 1139-1145Keiser, J., Efficacy and safety of artemether against a natural Fasciola hepatica infection in sheep (2008) Parasitology Research, 103 (3), pp. 517-522Keiser, J., Utzinger, J., Food-borne trematodiasis: Current chemotherapy and advances with artemisinins and synthetic trioxolanes (2007) Trends In Parasitology, 23 (11), pp. 555-562Klayman, D.L., Qinghaosu (Artemisinin): Na antimalarial drug from China (1985) Science, 228 (4703), pp. 1049-1055La-Scalea, M.A., Silva, H.S.R.C., Ferreira, E., Reducao voltametrica de artemisinina e sua interacao com grupo heme (hemina) (2007) Brazilian Journal of Pharmaceutical Sciences, 43 (3), pp. 371-383Martins, R.M., Estudio in vitro de la accion acaricida del aceite esencial de la graminea Citronela de Java (Cymbopogon winterianus Jowitt) en la garrapata Boophilus microplus (2006) Revista Brasileira De Plantas Medicinais De Botucatu, 8 (2), pp. 71-78Meshnick, S.R., Second-generation antimamalarial endoperoxides (1996) Parasitology Today, 12 (2), pp. 79-82Ribeiro, V.L.S., Acaricidal properties of extracts from the aerial parts of Hypericum polyanthemum on the cattle tick Boophilus microplus (2007) Veterinary Parasitology, 147 (1-2), pp. 199-203Rodrigues, R.A.F., Otimizacao do processo de extracao e isolamento do antimalarico artemisinina a partir de Artemisia annua L (2006) Química Nova, 29 (2), pp. 368-372Silva, H.S.R.C., (2006) Antimaláricos Potenciais: Pró-fármacos Poliméricos E Formas De Liberação Controlada De Artemisinina, p. 209. , Tese (Doutorado)-Universidade de Sao Paulo, Sao PauloSimoes, C.M., (2004) Farmacognosia: Da Planta Ao Medicamento, p. 1102. , 5. ed. Porto Alegre: Editora da UFRGSFlorianopolis: Editora da UFSCTaranto, A.G., Estudo sobre o mecanismo de acao da artemisinina e dos endoperoxidos, a mais nova classe de agents antimalaricos (2006) Sitientibus, 34, pp. 47-58Tariq, K.A., Anthelmintic activity of extracts of Artemisia absinthium against ovine nematodes (2009) Veterinary Parasitology, 160 (1-2), pp. 83-8

Search for leptophobic Z ' bosons decaying into four-lepton final states in proton-proton collisions at root s=8 TeV

Peer reviewe

Search for black holes and other new phenomena in high-multiplicity final states in proton-proton collisions at root s=13 TeV

Peer reviewe

Search for high-mass diphoton resonances in proton-proton collisions at 13 TeV and combination with 8 TeV search

Peer reviewe

Search for heavy resonances decaying into a vector boson and a Higgs boson in final states with charged leptons, neutrinos, and b quarks

Peer reviewe

Measurement of the Z boson differential cross section in transverse momentum and rapidity in proton-proton collisions at 8 TeV

We present a measurement of the Z boson differential cross section in rapidity and transverse momentum using a data sample of pp collision events at a centre-of-mass energy s=8 TeV, corresponding to an integrated luminosity of 19.7 fb-1. The Z boson is identified via its decay to a pair of muons. The measurement provides a precision test of quantum chromodynamics over a large region of phase space. In addition, due to the small experimental uncertainties in the measurement the data has the potential to constrain the gluon parton distribution function in the kinematic regime important for Higgs boson production via gluon fusion. The results agree with the next-to-next-to-leading-order predictions computed with the fewz program. The results are also compared to the commonly used leading-order MadGraph and next-to-leading-order powheg generators. © 2015 CERN for the benefit of the CMS Collaboration

Search for neutral resonances decaying into a Z boson and a pair of b jets or τ leptons

A search is performed for a new resonance decaying into a lighter resonance and a Z boson. Two channels are studied, targeting the decay of the lighter resonance into either a pair of oppositely charged τ leptons or a bb‾ pair. The Z boson is identified via its decays to electrons or muons. The search exploits data collected by the CMS experiment at a centre-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 19.8 fb −1 . No significant deviations are observed from the standard model expectation and limits are set on production cross sections and parameters of two-Higgs-doublet models