High temperature and high pressure plasma electrolysis experiments

Ohmori et al. (1), then Mizuno et al. (2,3) showed in high voltage-high current electrolysis experiments the production of excess heat and abnormal production of hydrogen. Cirillo et al. (4) have shown transmutation of elements on the tungsten cathode during similar types of plasma electrolysis. Also, Fauvarque et al. (5) have shown the production of excess heat. All these experiments have been performed with light water at boiling temperature i.e. 100[degrees]C and atmospheric pressure. We have developed a new calorimeter that can operate at higher temperatures and higher pressures. The cell is of cylindrical shape made of Teflon 10 cm in diameter and 21 cm high. The cell is positioned on a balance in order to measure continuously the weight loss of the cell during operation. The tungsten cathode of various diameters is located at the center of the cell, and the anode is a stainless steel foil surrounding the cathode. A mechanical pressure gauge permits the measurement of the pressure of the cell. The temperature is calculated from the pressure temperature boiling curve. A calibrated relief valve keeps the pressure constant in the cell. The electrical input power is measured by a high-speed wattmeter, and the heat produced is calculated from the weight loss of the cell. The cell is also equipped with a resistor for calibration of the system, and heating the water to boiling prior to the start of the electrolysis. At the conference we will give the new results showing the influence of the pressure and the temperature to the excess heat

Neonate Intestinal Immune Response to CpG Oligodeoxynucleotide Stimulation

Background: The development of mucosal vaccines is crucial to efficiently control infectious agents for which mucosae are the primary site of entry. Major drawbacks of these protective strategies are the lack of effective mucosal adjuvant. Synthetic oligodeoxynucleotides that contain several unmethylated cytosine-guanine dinucleotide (CpG-ODN) motifs are now recognized as promising adjuvants displaying mucosal adjuvant activity through direct activation of TLR9-expressing cells. However, little is known about the efficacy of these molecules in stimulating the intestinal immune system in neonates. Methodology/Principal Findings: First, newborn mice received CpG-ODN orally, and the intestinal cytokine and chemokine response was measured. We observed that oral administration of CpG-ODN induces CXC and CC chemokine responses and a cellular infiltration in the intestine of neonates as detected by immunohistochemistry. We next compared the efficiency of the oral route to intraperitoneal administration in stimulating the intestinal immune responses of both adults and neonates. Neonates were more responsive to TLR9-stimulation than adults whatever the CpG-ODN administration route. Their intestinal epithelial cells (IECs) indirectly responded to TLR9 stimulation and contributed to the CXC chemokine response, whereas other TLR9-bearing cells of the lamina-propria produced CC chemokines and Th1-type cytokines. Moreover, we showed that the intestine of adult exhibited a significantly higher level of IL10 at homeostasis than neonates, which might be responsible for the unresponsiveness to TLR9-stimulation, as confirmed by our findings in IL10-deficient mice. Conclusions/Significance: This is the first report that deciphers the role played by CpG-ODN in the intestine of neonates. This work clearly demonstrates that an intraperitoneal administration of CpG-ODN is more efficient in neonates than in adults to stimulate an intestinal chemokine response due to their lower IL-10 intestinal level. In addition we report the efficiency of the oral route at inducing intestinal chemokine responses in neonate that might be taken into consideration for further vaccine development against neonatal diseases

Mid-Neogene Mediterranean marine-continental correlations: an alternative interpretation

Recent revised magnetostratigraphic and astrochronologic calibrations of several calcareous planktonic microfossil datum events (in particular Coccolithus miopelagicus and Neogloboquadrina acostaensis) combined with our magnetobiostratigraphic investigation of a 10 m-thick section at Ecotet, near Lyon (France), in which terrestrial micromammals (Vallesian Zone MN9) are intercalated with calcareous nannoplankton and planktonic foraminifera belonging to Zones NN6-NN8 and N14-15 (=M11 and M12), respectively, lead us to question the recent recalibration by Krijgsman et al. [(1996) Eart Planet. Sci. Lett. 142, 367-380) of the Aragonian/Vallesian (MN7-8/MN9) boundary with Chron C5r.1r (~11.1 Ma) and the lower/upper Vallesian (MN9/10) boundary with Chron C4Ar.2n (~9.6-9.7 Ma). Ecotet, with a uniform reverse polarity, is shown to be assignable uniquely to Chron C5r. Interregional correlation of Ecotet with Mediterranean terrestrial stratigraphies indicates that Progonomys (whose First Occurrence (FO) has been traditionally placed in Mediterranean Mammal Zone MN10) was already present in Mediterranean Zone MN9 at ~11.4 Ma and did not experience a 2 million-year delay in its putative prochoresis from Asia

The Messinian Salinity Crisis in the Dacic Basin (SW Romania) and early Zanclean Mediterranean-Eastern Paratethys high sea-level connection

International audienceNew field observations and fossil analyses complete and clarify the strong impact of the Mediterranean sea-level changes linked to the peak of the Messinian Salinity Crisis on the Dacic Basin in southwestern Romania. In addition to the Gilbert-type fan delta already evidenced along the Danube River in the area of Turnu Severin, a new Gilbert-type fan delta is described northward. Early Zanclean bottomset beds are evidenced and dated based on nannofossils at the junction of the two coalescing Gilbert-type fan deltas. A clear sedimentological, morphological and chronologic differentiation is established in the area between the Carpathians Late Miocene piedmont alluvial fans and the early Zanclean Gilbert-type fan deltas. The early Zanclean age of the Hinova clays, where the bottomset beds of the Gilbert-type fan deltas are mostly developed, is confirmed by the occurrence of nannofossil markers of Subzone NN12b and a Bosphorian mollusk macrofauna. Early Zanclean inflow of Mediterranean marine waters into the Dacic Basin is also supported by the record of planktonic foraminifers. In the Dacic Basin, the Messinian Salinity Crisis resulted in the cutting of the Iron Gates by a Carpathians river. Fluvial erosion also affected the residual Pannonian Basin and probably catched the paleo-Tisza River which contributed to the erosion of the Iron Gates and to the fluvial drainage of the partly desiccated Dacic Basin. Arguments are reinforced in favor of a marine gateway between the Mediterranean and Dacic Basin through the Balkans before and after the Messinian Salinity Crisis

Origin of the high elevated Pyrenean peneplain

Peneplanation of mountain ranges is generally considered the result of long-term erosional processes that smooth relief and lower elevation near sea level. Therefore peneplain remnants at high elevation in mountain ranges are used to infer posttectonic surface uplift. Such an interpretation has been proposed for the Pyrenees where high-elevation, low-relief erosional surfaces rose up to more than 2000 m. Because the Pyrenean foreland basins are filled with very thick continental deposits, which have buried the early jagged landscape, we challenge this hypothesis by pointing out that relief applanation does not necessarily require elevation lowering. We propose an alternative interpretation in which piedmont aggradation of detrital sediment that comes from erosion of the high chain induces the rise of the base level of the range, therefore reducing strongly the erosive efficiency of the drainage system and resulting in the progressive smoothing of the relief. Such a process allows a high-elevation, low-relief erosional surface to develop at the scale of the range. In the Pyrenees, occurrence of high-elevation, low-relief erosional surface remnants does not imply a posttectonic uplift, but is instead due to the dissection of the initial Miocene high-elevation, low-relief surface by the recent drainage system, the erosive activity of which has been enhanced by global climate change from the late Pliocene onward

New insights on the Sorbas Basin (SE Spain): the onshore reference of the Messinian Salinity Crisis

International audienceThe Sorbas Basin is the land reference of the Messinian Salinity Crisis (MSC) that affected the Mediterranean Sea in the latest Miocene. Its stratigraphy has been re-visited using calcareous nannofossils and planktonic foraminifers, which provide a reliable biostratigraphic frame and lead to particularly specify the relationships between the Sorbas and Zorreras members with Yesares evaporites.The evaporites overlie a shallowing upward sequence ending with the deposition of the Reef Unit and Terminal Carbonate Complex (TCC) on the periphery of the basin. The reefal carbonates of the TCC are overlain by clastic deposits that are foreset beds of post-MSC Gilbert-type fan deltas developed on the northern edge of the basin. These sedimentary structures are separated from reefal carbonates and the Reef Unit by the Messinian Erosional Surface (MES). The various facies of the Sorbas Member have been correlated with the bottomset beds of the Gilbert-type fan deltas despite some differences in palaeobathymetry. In the southeastern periphery of the basin, the MES separates the Sorbas Member from the Yesares gypsums. In the central part of the basin, a hiatus characterizes the contact between these members. The Zorreras Member postdates the MSC and entirely belongs to Zanclean. Its white “Lago Mare” layers are lagoonal deposits, the fauna of which is confirmed to result from Mediterranean–Paratethys high sea-level exchange after the post-MSC marine reflooding of the Mediterranean Basin.This study allows to re-assert the two-step scenario of the MSC (Clauzon et al., 1996) with the following events:- at 5.971–5.600 Ma, minor sea-level fall resulting in the desiccation of this peripheral basin with secondary fluctuations;- at 5.600–5.460 Ma, significant subaerial erosion (or lack of sedimentation) caused by the almost complete desiccation of the Mediterranean Sea;- instantaneous marine reflooding, accepted at 5.460 Ma, followed by continuing sea-level rise

New Insights on the Sorbas Basin (SE Spain): the onshore reference of the Messinian Salinity Crisis

The Sorbas Basin is the land reference of the Messinian Salinity Crisis (MSC) that affected the Mediterranean Sea in the latest Miocene. Its stratigraphy has been re-visited using calcareous nannofossils and planktonic foraminifers, which provide a reliable biostratigraphic frame and lead to particularly specify the relationships between the Sorbas and Zorreras members with Yesares evaporites. The evaporites overlie a shallowing upward sequence ending with the deposition of the Reef Unit and Terminal Carbonate Complex (TCC) on the periphery of the basin. The reefal carbonates of the TCC are overlain by clastic deposits that are foreset beds of post-MSC Gilbert-type fan deltas developed on the northern edge of the basin. These sedimentary structures are separated from reefal carbonates and the Reef Unit by the Messinian Erosional Surface (MES). The various facies of the Sorbas Member have been correlated with the bottomset beds of the Gilbert-type fan deltas despite some differences in palaeobathymetry. In the southeastern periphery of the basin, the MES separates the Sorbas Member from the Yesares gypsums. In the central part of the basin, a hiatus characterizes the contact between these members. The Zorreras Member postdates the MSC and entirely belongs to Zanclean. Its white "Lago Mare" layers are lagoonal deposits, the fauna of which is confirmed to result from Mediterranean-Paratethys high sea-level exchange after the post-MSC marine reflooding of the Mediterranean Basin. This study allows to re-assert the two-step scenario of the MSC (Clauzon et al., 1996) with the following events: - at 5.971-5.600 Ma, minor sea-level fall resulting in the desiccation of this peripheral basin with secondary fluctuations; - at 5.600-5.460 Ma, significant subaerial erosion (or lack of sedimentation) caused by the almost complete desiccation of the Mediterranean Sea; - instantaneous marine reflooding, accepted at 5.460 Ma, followed by continuing sea-level rise

The Miocene – Pliocene boundary and the Messinian Salinity Crisis in the easternmost Mediterranean: insights from the Hatay Graben (Southern Turkey).

The Hatay Graben is one of three easternmost basins in the Mediterranean that preserve sediments that span the Miocene-Pliocene boundary, including gypsums from the Messinian Salinity Crisis (MSC). Here we integrate existing data and present new sedimentological and micropalaeontological data to investigate the palaeoenvironments of late Miocene to early Pliocene deposits and place this important area into a regional stratigraphic framework. Six sections are described along a ~ W – E transect illustrating the key features of this time period. Late Miocene (Pre-MSC) sediments are characterised by open marine marls with a benthic foraminiferal fauna suggestive of water depths of 100 – 200 m or less. Primary lower gypsum deposits are determined to be absent from the graben as sedimentological and strontium isotopes are characteristic of the resedimented lower gypsums. The intervening Messinian erosion surface is preserved near the basin margins as an unconformity but appears to be a correlative conformity in the basin depocentre. No Upper Gypsums or ‘Lago–Mare’ facies have been identified but available data do tentatively suggest a return to marine conditions in the basin prior to the Zanclean boundary. Sediments stratigraphically overlying the Messinian gypsums and marls are coarse-grained sandstones from coastal and Gilbert-type delta depositional environments. The Hatay Graben is not only strikingly similar to Messinian basins on nearby Cyprus but also to the overall model for the MSC, demonstrating the remarkable consistency of palaeoenvironments found in marginal basins across the region at this time