A Spitzer Census of Star Formation Activity in the Pipe Nebula

The Pipe Nebula, a large nearby molecular cloud lacks obvious signposts of star formation in all but one of more than 130 dust extinction cores that have been identified within it. In order to quantitatively determine the current level of star formation activity in the Pipe Nebula, we analyzed 13 square degrees of sensitive mid-infrared maps of the entire cloud, obtained with the Multiband Imaging Photometer for Spitzer (MIPS) at wavelengths of 24 micron and 70 micron to search for candidate Young Stellar Objects (YSOs) in the high-extinction regions. We argue that our search is complete for class I and typical class II YSOs with luminosities of Lbol~0.2 Lo and greater. We find only 18 candidate YSOs in the high-extinction regions of the entire Pipe cloud. Twelve of these sources are previously known members of a small cluster associated with Barnard 59, the largest and most massive dense core in the cloud. With only six candidate class I and class II YSOs detected towards extinction cores outside of this cluster, our findings emphatically confirm the notion of an extremely low level of star formation activity in the Pipe Nebula. The resulting star formation efficiency for the entire cloud mass is only ~0.06 %.Comment: 23 pages, 8 figures, accepted for publication in Ap

The Age, Stellar Content and Star Formation Timescale of the B59 Dense Core

We have used moderate resolution, near-infrared spectra from the SpeX spectrograph on the NASA Infrared Telescope facility to characterize the stellar content of Barnard 59 (B59), the most active star-forming core in the Pipe Nebula. Measuring luminosity and temperature sensitive features in the spectra of 20 candidate YSOs, we identified likely background giant stars and measured each star's spectral type, extinction, and NIR continuum excess. We find that B59 is composed of late type (K4-M6) low-mass (0.9--0.1 M_sun) YSOs whose median stellar age is comparable to, if not slightly older than, that of YSOs within the Rho Oph, Taurus, and Chameleon star forming regions. Deriving absolute age estimates from pre-main sequence models computed by D'Antona et al., and accounting only for statistical uncertainties, we measure B59's median stellar age to be 2.6+/-0.8 Myrs. Including potential systematic effects increases the error budget for B59's median (DM98) stellar age to 2.6+4.1/-2.6 Myrs. We also find that the relative age orderings implied by pre-main sequence evolutionary tracks depend on the range of stellar masses sampled, as model isochrones possess significantly different mass dependencies. The maximum likelihood median stellar age we measure for B59, and the region's observed gas properties, suggest that the B59 dense core has been stable against global collapse for roughly 6 dynamical timescales, and is actively forming stars with a star formation efficiency per dynamical time of ~6%. This maximum likelihood value agrees well with recent star formation simulations that incorporate various forms of support against collapse, such as sub-critical magnetic fields, outflows, and radiative feedback from protostellar heating. [abridged]Comment: 20 pages, 12 figures, accepted for publication in the Astrophysical Journal; updated to amend acknowledgment

Filamentary structure of star-forming complexes

The nearest young stellar groups are associated with "hubs" of column density exceeding 10^22 cm^-2, according to recent observations. These hubs radiate multiple "filaments" of parsec length, having lower column density and fewer stars. Systems with many filaments tend to have parallel filaments with similar spacing. Such "hub-filament structure" is associated with all of the nine young stellar groups within 300 pc, forming low-mass stars. Similar properties are seen in infrared dark clouds forming more massive stars. In a new model, an initial clump in a uniform medium is compressed into a self-gravitating, modulated layer. The outer layer resembles the modulated equilibrium of Schmid-Burgk (1967) with nearly parallel filaments. The filaments converge onto the compressed clump, which collapses to form stars with high efficiency. The initial medium and condensations have densities similar to those in nearby star-forming clouds and clumps. The predicted structures resemble observed hub-filament systems in their size, shape, and column density, and in the appearance of their filaments. These results suggest that hub-filament structure associated with young stellar groups may arise from compression of clumpy gas in molecular clouds.Comment: accepted by ApJ, to appear August 10, 200

Rapid Determination of Myosin Heavy Chain Expression in Rat, Mouse, and Human Skeletal Muscle Using Multicolor Immunofluorescence Analysis

Skeletal muscle is a heterogeneous tissue comprised of fibers with different morphological, functional, and metabolic properties. Different muscles contain varying proportions of fiber types; therefore, accurate identification is important. A number of histochemical methods are used to determine muscle fiber type; however, these techniques have several disadvantages. Immunofluorescence analysis is a sensitive method that allows for simultaneous evaluation of multiple MHC isoforms on a large number of fibers on a single cross-section, and offers a more precise means of identifying fiber types. In this investigation we characterized pure and hybrid fiber type distribution in 10 rat and 10 mouse skeletal muscles, as well as human vastus lateralis (VL) using multicolor immunofluorescence analysis. In addition, we determined fiber type-specific cross-sectional area (CSA), succinate dehydrogenase (SDH) activity, and α-glycerophosphate dehydrogenase (GPD) activity. Using this procedure we were able to easily identify pure and hybrid fiber populations in rat, mouse, and human muscle. Hybrid fibers were identified in all species and made up a significant portion of the total population in some rat and mouse muscles. For example, rat mixed gastrocnemius (MG) contained 12.2% hybrid fibers whereas mouse white tibialis anterior (WTA) contained 12.1% hybrid fibers. Collectively, we outline a simple and time-efficient method for determining MHC expression in skeletal muscle of multiple species. In addition, we provide a useful resource of the pure and hybrid fiber type distribution, fiber CSA, and relative fiber type-specific SDH and GPD activity in a number of rat and mouse muscles

Relationship among fibre type, myosin ATPase activity and contractile properties

At least two types of skeletal muscle myosin have been described which differ in ATPase activity and stability in alkaline or acidic media. Differences in ATPase characteristics distinguish Type I and Type II fibres histochemically. In this study, ATPase activity of myosin from muscles of several species with known histochemical and contractile properties has been determined to test the hypothesis that (1) myosin ATPase activity, (2) histochemical determination of fibre types and (3) maximum shortening velocity, all provide equivalent estimates of contractile properties in muscles of mixed fibre types. Maximum shortening velocity appears to be proportional to ATPase activity as expected from previous reports by Barany. However, both myosin ATPase and the maximum shortening velocity exhibit curvilinear relationships to the fraction of cross-sectional area occupied by Type II fibres. Therefore, we reject the hypothesis and conclude that histochemically determined myofibrillar ATPase does not accurately reflect the intrinsic ATPase activity or shortening velocity in muscles of mixed fibre types. Our data are consistent with the presence of more than two myosin isozymes or with a mixture of isozymes within single muscle fibres.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42851/1/10735_2005_Article_BF01005238.pd

Elective cancer surgery in COVID-19-free surgical pathways during the SARS-CoV-2 pandemic: An international, multicenter, comparative cohort study

PURPOSE As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19–free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19–free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19–free surgical pathways. Patients who underwent surgery within COVID-19–free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19–free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score–matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19–free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION Within available resources, dedicated COVID-19–free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks

Elective Cancer Surgery in COVID-19-Free Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study.

PURPOSE: As cancer surgery restarts after the first COVID-19 wave, health care providers urgently require data to determine where elective surgery is best performed. This study aimed to determine whether COVID-19-free surgical pathways were associated with lower postoperative pulmonary complication rates compared with hospitals with no defined pathway. PATIENTS AND METHODS: This international, multicenter cohort study included patients who underwent elective surgery for 10 solid cancer types without preoperative suspicion of SARS-CoV-2. Participating hospitals included patients from local emergence of SARS-CoV-2 until April 19, 2020. At the time of surgery, hospitals were defined as having a COVID-19-free surgical pathway (complete segregation of the operating theater, critical care, and inpatient ward areas) or no defined pathway (incomplete or no segregation, areas shared with patients with COVID-19). The primary outcome was 30-day postoperative pulmonary complications (pneumonia, acute respiratory distress syndrome, unexpected ventilation). RESULTS: Of 9,171 patients from 447 hospitals in 55 countries, 2,481 were operated on in COVID-19-free surgical pathways. Patients who underwent surgery within COVID-19-free surgical pathways were younger with fewer comorbidities than those in hospitals with no defined pathway but with similar proportions of major surgery. After adjustment, pulmonary complication rates were lower with COVID-19-free surgical pathways (2.2% v 4.9%; adjusted odds ratio [aOR], 0.62; 95% CI, 0.44 to 0.86). This was consistent in sensitivity analyses for low-risk patients (American Society of Anesthesiologists grade 1/2), propensity score-matched models, and patients with negative SARS-CoV-2 preoperative tests. The postoperative SARS-CoV-2 infection rate was also lower in COVID-19-free surgical pathways (2.1% v 3.6%; aOR, 0.53; 95% CI, 0.36 to 0.76). CONCLUSION: Within available resources, dedicated COVID-19-free surgical pathways should be established to provide safe elective cancer surgery during current and before future SARS-CoV-2 outbreaks