Obesity: Prevalence, Theories, Medical Consequences, Management, and Research Directions

Obesity and its associated disorders are a growing epidemic across the world. Many genetic, physiological, and behavioral factors play a role in the etiology of obesity. Diet and exercise are known to play a valuable role in the treatment and prevention of obesity and associated disorders such as hypertension, heart disease, and diabetes. Therefore, the purpose of this review is to examine the prevalence, etiology, consequences, and treatment of obesity

Who Will Volunteer? Religiosity, Everyday Racism, and Social Participation Among African American Men

This study explores the relative importance of everyday racism, empathic concern, communalism, and religiosity as predictors of pro-social involvement (i.e., volunteerism and membership in political/social justice organizations) among a sample of African American men ( N = 151). Church involvement emerged as a positive predictor of the likelihood that these men were involved in volunteer work as well as the number of hours that men dedicated to volunteer work. Communalism positively predicted the amount of time (in hours per year) that men were involved in volunteer work. Subjective religiosity and the stress of everyday racism were associated with a greater likelihood of being a member of a political–social justice organization. Implications of these findings are discussed.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/44632/1/10804_2004_Article_496119.pd

Cognitive Rehabilitation With Mobile Technology and Social Support for Veterans With TBI and PTSD: A Randomized Clinical Trial.

OBJECTIVE: To investigate effects of cognitive rehabilitation with mobile technology and social support on veterans with traumatic brain injury (TBI) and posttraumatic stress disorder (PTSD). PARTICIPANTS: There were 112 dyads, comprised by a veteran and a family member or friend (224 participants in total). DESIGN: Dyads were randomized to the following: (1) a novel intervention, Cognitive Applications for Life Management (CALM), involving goal management training plus mobile devices for cueing and training attentional control; or (2) Brain Health Training, involving psychoeducation plus mobile devices to train visual memory. MAIN MEASURES: Executive dysfunction (disinhibition, impulsivity) and emotional dysregulation (anger, maladaptive interpersonal behaviors) collected prior to randomization and following intervention completion at 6 months. RESULTS: The clinical trial yielded negative findings regarding executive dysfunction but positive findings on measures of emotion dysregulation. Veterans randomized to CALM reported a 25% decrease in anger over 6 months compared with 8% reduction in the control (B = -5.27, P = .008). Family/friends reported that veterans randomized to CALM engaged in 26% fewer maladaptive interpersonal behaviors (eg, aggression) over 6 months compared with 6% reduction in the control (B = -2.08, P = .016). An unanticipated result was clinically meaningful change in reduced PTSD symptoms among veterans randomized to CALM (P < .001). CONCLUSION: This preliminary study demonstrated effectiveness of CALM for reducing emotional dysregulation in veterans with TBI and PTSD

Minimal information for studies of extracellular vesicles 2018 (MISEV2018):a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points

Effects of a multicomponent school-based intervention on health markers, body composition, physical fitness, and psychological measures in overweight and obese adolescent females.

Includes bibliographic references (p. 182-200)The purpose of this study was to determine whether participation in a multicomponent school-based intervention using circuit-resistance training, dietary intervention and behavioral modification had an effect on health markers, body composition, physical fitness, and psychological measures for overweight/obese adolescent females. Forty-two overweight/obese adolescents participated in the 10-week program with assessments completed at 0, 10, and 22 weeks. Data were analyzed by ANOVA repeated measures analysis, with a level of significance set at p < 0.05, and presented as means ± SD from baseline. Statistically significant group x time interactions were observed for the following: body mass (CON 2.82% ± 2.7 vs. EXP 0.58% ± 2.5, p = 0.021) fat mass (CON 3.64% ± 5.9 vs. EXP -2.66% ± 5.7, p = 0.006), percent fat (CON 0.52% ± 4.69 vs. EXP -2.92% ± 4.44, p = 0.024), triglycerides (CON 44.78% ± 85.11 vs. EXP -9.56% ± 34.99, p = 0.002), HOMA-IR (CON 49.41% ± 69.65 vs. EXP 17.64% ± 66.76, p = 0.052), physical activity environmental factors (CON -6.92% ± 18.66 vs. EXP 9.96% ± 27.79, p = 0.039), fruit and vegetable con decisions (CON 31.71% ± 49.75 vs. EXP 3.41% ± 47.22, p = 0.043), and dietary protein intake (CON -3.28% ± 53.05 vs. EXP 8.15% ± 58.68, p = 0.025). Time significance represented an overall mean increase in body mass (1.6 % ± 3), lean mass (2.7% ± 4), bone mineral content (3.6% ± 5) and bone mineral density (3.4% ± 3). Follow-up testing was conducted with a subset of participants (n = 29), after three months. The participants’ revealed statistically significant group x time effects for the pediatric quality of life psychosocial health summary score (CON -23% ± 40 vs. EXP 25.3% ± 65, p = 0.039) and the PACE measurements of physical activity family support (CON -0.64% ± 24.4 vs. EXP 17.8% ± 19.3, p = 0.027). This research indicated that overweight/obese adolescent females can benefit from both standard physical education and a multicomponent program. However, the participants in the multicomponent program observed significantly greater improvements in body composition, triglycerides, and insulin sensitivity.by Jacqueline Beckham Dove.Ph.D

TIA-1 RRM23 binding and recognition of target oligonucleotides

TIA-1 (T-cell restricted intracellular antigen-1) is an RNA-binding protein involved in splicing and translational repression. It mainly interacts with RNA via its second and third RNA recognition motifs (RRMs), with specificity for U-rich sequences directed by RRM2. It has recently been shown that RRM3 also contributes to binding, with preferential binding for C-rich sequences. Here we designed UC-rich and CU-rich 10-nt sequences for engagement of both RRM2 and RRM3 and demonstrated that the TIA-1 RRM23 construct preferentially binds the UC-rich RNA ligand (5΄-UUUUUACUCC-3΄). Interestingly, this binding depends on the presence of Lys274 that is C-terminal to RRM3 and binding to equivalent DNA sequences occurs with similar affinity. Small-angle X-ray scattering was used to demonstrate that, upon complex formation with target RNA or DNA, TIA-1 RRM23 adopts a compact structure, showing that both RRMs engage with the target 10-nt sequences to form the complex. We also report the crystal structure of TIA-1 RRM2 in complex with DNA to 2.3 Å resolution providing the first atomic resolution structure of any TIA protein RRM in complex with oligonucleotide. Together our data support a specific mode of TIA-1 RRM23 interaction with target oligonucleotides consistent with the role of TIA-1 in binding RNA to regulate gene expression.Peer reviewe

Structure of the PCBP2/stem-loop IV complex underlying translation initiation mediated by the poliovirus type I IRES.

The poliovirus type I IRES is able to recruit ribosomal machinery only in the presence of host factor PCBP2 that binds to stem-loop IV of the IRES. When PCBP2 is cleaved in its linker region by viral proteinase 3CD, translation initiation ceases allowing the next stage of replication to commence. Here, we investigate the interaction of PCBP2 with the apical region of stem-loop IV (SLIVm) of poliovirus RNA in its full-length and truncated form. CryoEM structure reconstruction of the full-length PCBP2 in complex with SLIVm solved to 6.1 Å resolution reveals a compact globular complex of PCBP2 interacting with the cruciform RNA via KH domains and featuring a prominent GNRA tetraloop. SEC-SAXS, SHAPE and hydroxyl-radical cleavage establish that PCBP2 stabilizes the SLIVm structure, but upon cleavage in the linker domain the complex becomes more flexible and base accessible. Limited proteolysis and REMSA demonstrate the accessibility of the linker region in the PCBP2/SLIVm complex and consequent loss of affinity of PCBP2 for the SLIVm upon cleavage. Together this study sheds light on the structural features of the PCBP2/SLIV complex vital for ribosomal docking, and the way in which this key functional interaction is regulated following translation of the poliovirus genome