65 research outputs found
Reparación de las fracturas de huesos largos en traumatismos craneoencefálicos: estudio experimental en la rata
Los pacientes con traumatismo craneoencefálico presentan una respuesta osteogénica
alterada. Para estudiar si existe o no un callo de fractura más voluminoso o más prematuro
en las fracturas de hueso largo se ha buscado un modelo experimental en rata Wistar con y sin
lesión encefálica. El estudio no demuestra diferencias significativas. Se discute la importancia de
factores terapéuticos más que el hecho del traumatismo creaneoencefálico.Osteogenic response deviates significantly in patients with traumatic brain injury.
In this study, we analyze if there is an increased volume or earlier callus formation in long bones
fractures in Wistar rats with and without brain injury. The results show no significant difference.
The importance of some therapeutical aspects other than head injury are discussed
Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory
Objectives Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the in vitro studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied. Methods We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis. Results Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria. Conclusions The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.This work was funded by the following grants from the Spanish MINECO (MAT2013-
48224-C2-2-R and MAT2013-48224-C2-1-R).
M. Martínez-Pérez reports funding received from EFORT 2015 congress: travel supported
by PFIZER, which is related to this article. J. Esteban and E. Gómez-Barrena report funding received from several companies
for travel, expenses and grants, none of which is related to this articl
The Sloan Great Wall. Rich clusters
We present the results of the study of the substructure and galaxy content of
ten rich clusters of galaxies in three different superclusters of the Sloan
Great Wall. We determine the substructure in clusters using the 'Mclust'
package from the 'R' statistical environment and analyse their galaxy content.
We analyse the distribution of the peculiar velocities of galaxies in clusters
and calculate the peculiar velocity of the first ranked galaxy. We show that
clusters in our sample have more than one component; in some clusters different
components also have different galaxy content. We find that in some clusters
with substructure the peculiar velocities of the first ranked galaxies are
large. All clusters in our sample host luminous red galaxies. They can be found
both in the central areas of clusters as well as in the outskirts, some of them
have large peculiar velocities. About 1/3 of red galaxies in clusters are
spirals. The scatter of colours of red ellipticals is in most clusters larger
than that of red spirals. The presence of substructure in rich clusters, signs
of possible mergers and infall, as well as the large peculiar velocities of the
first ranked galaxies suggest that the clusters in our sample are not yet
virialized. We present merger trees of dark matter haloes in an N-body
simulation to demonstrate the formation of present-day dark matter haloes via
multiple mergers during their evolution. In simulated dark matter haloes we
find a substructure similar to that in observed clusters.Comment: 19 pages, 44 figures, accepted for publication in Astronomy and
Astrophysic
Multimodality in galaxy clusters from SDSS DR8: substructure and velocity distribution
We search for the presence of substructure, a non-Gaussian, asymmetrical
velocity distribution of galaxies, and large peculiar velocities of the main
galaxies in galaxy clusters with at least 50 member galaxies, drawn from the
SDSS DR8. We employ a number of 3D, 2D, and 1D tests to analyse the
distribution of galaxies in clusters: 3D normal mixture modelling, the
Dressler-Shectman test, the Anderson-Darling and Shapiro-Wilk tests and others.
We find the peculiar velocities of the main galaxies, and use principal
component analysis to characterise our results. More than 80% of the clusters
in our sample have substructure according to 3D normal mixture modelling, the
Dressler-Shectman (DS) test shows substructure in about 70% of the clusters.
The median value of the peculiar velocities of the main galaxies in clusters is
206 km/s (41% of the rms velocity). The velocities of galaxies in more than 20%
of the clusters show significant non-Gaussianity. While multidimensional normal
mixture modelling is more sensitive than the DS test in resolving substructure
in the sky distribution of cluster galaxies, the DS test determines better
substructure expressed as tails in the velocity distribution of galaxies.
Richer, larger, and more luminous clusters have larger amount of substructure
and larger (compared to the rms velocity) peculiar velocities of the main
galaxies. Principal component analysis of both the substructure indicators and
the physical parameters of clusters shows that galaxy clusters are complicated
objects, the properties of which cannot be explained with a small number of
parameters or delimited by one single test. The presence of substructure, the
non-Gaussian velocity distributions, as well as the large peculiar velocities
of the main galaxies, shows that most of the clusters in our sample are
dynamically young.Comment: 15 pages, 11 figures, 2 online tables, accepted for publication in
Astronomy and Astrophysic
A SOS3 homologue maps to HvNax4, a barley locus controlling an environmentally sensitive Na+ exclusion trait
Genes that enable crops to limit Na+ accumulation in shoot tissues represent potential sources of salinity tolerance for breeding. In barley, the HvNax4 locus lowered shoot Na+ content by between 12% and 59% (g−1 DW), or not at all, depending on the growth conditions in hydroponics and a range of soil types, indicating a strong influence of environment on expression. HvNax4 was fine-mapped on the long arm of barley chromosome 1H. Corresponding intervals of ∼200 kb, containing a total of 34 predicted genes, were defined in the sequenced rice and Brachypodium genomes. HvCBL4, a close barley homologue of the SOS3 salinity tolerance gene of Arabidopsis, co-segregated with HvNax4. No difference in HvCBL4 mRNA expression was detected between the mapping parents. However, genomic and cDNA sequences of the HvCBL4 alleles were obtained, revealing a single Ala111Thr amino acid substitution difference in the encoded proteins. The known crystal structure of SOS3 was used as a template to obtain molecular models of the barley proteins, resulting in structures very similar to that of SOS3. The position in SOS3 corresponding to the barley substitution does not participate directly in Ca2+ binding, post-translational modifications or interaction with the SOS2 signalling partner. However, Thr111 but not Ala111 forms a predicted hydrogen bond with a neighbouring α-helix, which has potential implications for the overall structure and function of the barley protein. HvCBL4 therefore represents a candidate for HvNax4 that warrants further investigation
Pilot multi-omic analysis of human bile from benign and malignant biliary strictures: a machine-learning approach
Cholangiocarcinoma (CCA) and pancreatic adenocarcinoma (PDAC) may lead to the development of extrahepatic obstructive cholestasis. However, biliary stenoses can also be caused by benign conditions, and the identification of their etiology still remains a clinical challenge. We performed metabolomic and proteomic analyses of bile from patients with benign (n = 36) and malignant conditions, CCA (n = 36) or PDAC (n = 57), undergoing endoscopic retrograde cholangiopancreatography with the aim of characterizing bile composition in biliopancreatic disease and identifying biomarkers for the differential diagnosis of biliary strictures. Comprehensive analyses of lipids, bile acids and small molecules were carried out using mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (1H-NMR) in all patients. MS analysis of bile proteome was performed in five patients per group. We implemented artificial intelligence tools for the selection of biomarkers and algorithms with predictive capacity. Our machine-learning pipeline included the generation of synthetic data with properties of real data, the selection of potential biomarkers (metabolites or proteins) and their analysis with neural networks (NN). Selected biomarkers were then validated with real data. We identified panels of lipids (n = 10) and proteins (n = 5) that when analyzed with NN algorithms discriminated between patients with and without cancer with an unprecedented accuracy.This research was funded by: Instituto de Salud Carlos III (ISCIII) co-financed by Fondo Europeo de Desarrollo Regional (FEDER) Una manera de hacer Europa, grant numbers: PI16/01126 (M.A.A.), PI19/00819 (M.J.M. and J.J.G.M.), PI15/01132, PI18/01075 and Miguel Servet Program CON14/00129 (J.M.B.); Fundación Científica de la Asociación Española Contra el Cáncer (AECC Scientific Foundation), grant name: Rare Cancers 2017 (J.M.U., M.L.M., J.M.B., M.J.M., R.I.R.M., M.G.F.-B., C.B., M.A.A.); Gobierno de Navarra Salud, grant number 58/17 (J.M.U., M.A.A.); La Caixa Foundation, grant name: HEPACARE (C.B., M.A.A.); AMMF The Cholangiocarcinoma Charity, UK, grant number: 2018/117 (F.J.C. and M.A.A.); PSC Partners US, PSC Supports UK, grant number 06119JB (J.M.B.); Horizon 2020 (H2020) ESCALON project, grant number H2020-SC1-BHC-2018–2020 (J.M.B.); BIOEF (Basque Foundation for Innovation and Health Research: EiTB Maratoia, grant numbers BIO15/CA/016/BD (J.M.B.) and BIO15/CA/011 (M.A.A.). Department of Health of the Basque Country, grant number 2017111010 (J.M.B.). La Caixa Foundation, grant number: LCF/PR/HP17/52190004 (M.L.M.), Mineco-Feder, grant number SAF2017-87301-R (M.L.M.), Fundación BBVA grant name: Ayudas a Equipos de Investigación Científica Umbrella 2018 (M.L.M.). MCIU, grant number: Severo Ochoa Excellence Accreditation SEV-2016-0644 (M.L.M.). Part of the equipment used in this work was co-funded by the Generalitat Valenciana and European Regional Development Fund (FEDER) funds (PO FEDER of Comunitat Valenciana 2014–2020). Gobierno de Navarra fellowship to L.C. (Leticia Colyn); AECC post-doctoral fellowship to M.A.; Ramón y Cajal Program contracts RYC-2014-15242 and RYC2018-024475-1 to F.J.C. and M.G.F.-B., respectively. The generous support from: Fundación Eugenio Rodríguez Pascual, Fundación Echébano, Fundación Mario Losantos, Fundación M Torres and Mr. Eduardo Avila are acknowledged. The CNB-CSIC Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019/0001 (F.J.C.). Comunidad de Madrid Grant B2017/BMD-3817 (F.J.C.).Peer reviewe
Chemotherapy or allogeneic transplantation in high-risk Philadelphia chromosome–negative adult lymphoblastic leukemia
The need for allogeneic hematopoietic stem cell transplantation (allo-HSCT) in adults with Philadelphia chromosome–negative (Ph−) acute lymphoblastic leukemia (ALL) with high-risk (HR) features and adequate measurable residual disease (MRD) clearance remains unclear. The aim of the ALL-HR-11 trial was to evaluate the outcomes of HR Ph− adult ALL patients following chemotherapy or allo-HSCT administered based on end-induction and consolidation MRD levels. Patients aged 15 to 60 years with HR-ALL in complete response (CR) and MRD levels (centrally assessed by 8-color flow cytometry) <0.1% after induction and <0.01% after early consolidation were assigned to receive delayed consolidation and maintenance therapy up to 2 years in CR. The remaining patients were allocated to allo-HSCT. CR was attained in 315/348 patients (91%), with MRD <0.1% after induction in 220/289 patients (76%). By intention-to-treat, 218 patients were assigned to chemotherapy and 106 to allo-HSCT. The 5-year (±95% confidence interval) cumulative incidence of relapse (CIR), overall survival (OS), and event-free survival probabilities for the whole series were 43% ± 7%, 49% ± 7%, and 40% ± 6%, respectively, with CIR and OS rates of 45% ± 8% and 59% ± 9% for patients assigned to chemotherapy and of 40% ± 12% and 38% ± 11% for those assigned to allo-HSCT, respectively. Our results show that avoiding allo-HSCT does not hamper the outcomes of HR Ph− adult ALL patients up to 60 years with adequate MRD response after induction and consolidation. Better postremission alternative therapies are especially needed for patients with poor MRD clearance
Sustainable development and human resource management. A science mapping approach
Financiación ECO2017-82208-PThis manuscript presents a systematic review of "sustainable human resource man-agement"(HRM), highlighting its major themes and the evolution and tendenciesobserved in this field. It contributes to the development of this relatively new topic,offering a critical evaluation and identifying the highest impact research strands.The Web of Science database returned 111 documents spanning the period 1997-2018, and a conceptual science mapping analysis based on co-word bibliographic net-works was developed, using SciMAT as an analytical tool. The motor themes (welldeveloped and important for the structure of the discipline) in the field of sustainableHRM areenvironmental management,socially responsible HRM, andturnover.Employeeengagementis a specialised theme (well developed but less important for the struc-ture of the research field),human resource practicesis a basic or transversal theme(important for the discipline but not developed), andsustainable leadershipandenvi-ronmental performanceare emerging themes (both weakly developed and marginalto the field).Management and Marketing Department (University of Pablo de Olavide
Planck Intermediate Results. XXXVI. Optical identification and redshifts of Planck SZ sources with telescopes at the Canary Islands Observatories
We present the results of approximately three years of observations of Planck
Sunyaev-Zeldovich (SZ) sources with telescopes at the Canary Islands
observatories as part of the general optical follow-up programme undertaken by
the Planck collaboration. In total, 78 SZ sources are discussed. Deep-imaging
observations were obtained for most of these sources; spectroscopic
observations in either in long-slit or multi-object modes were obtained for
many. We effectively used 37.5 clear nights. We found optical counterparts for
73 of the 78 candidates. This sample includes 53 spectroscopic redshift
determinations, 20 of them obtained with a multi-object spectroscopic mode. The
sample contains new redshifts for 27 Planck clusters that were not included in
the first Planck SZ source catalogue (PSZ1).Comment: 15 pages, 14 figures, accepted for publication in A&
Planck 2013 results : XXXII. The updated Planck catalogue of Sunyaev-Zeldovich sources
We update the all-sky Planck catalogue of 1227 clusters and cluster candidates (PSZ1) published in March 2013, derived from detections of the Sunyaev-Zeldovich (SZ) effect using the first 15.5 months of Planck satellite observations. As an addendum, we deliver an updated version of the PSZ1 catalogue, reporting the further confirmation of 86 Planck-discovered clusters. In total, the PSZ1 now contains 947 confirmed clusters, of which 214 were confirmed as newly discovered clusters through follow-up observations undertaken by the Planck Collaboration. The updated PSZ1 contains redshifts for 913 systems, of which 736 (similar to 80.6%) are spectroscopic, and associated mass estimates derived from the Y-z mass proxy. We also provide a new SZ quality flag for the remaining 280 candidates. This flag was derived from a novel artificial neural-network classification of the SZ signal. Based on this assessment, the purity of the updated PSZ1 catalogue is estimated to be 94%. In this release, we provide the full updated catalogue and an additional readme file with further information on the Planck SZ detections.Peer reviewe
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