Molecular Profiling of Lymphatic Endothelial Cell Activation In Vitro

The lymphatic vascular system plays a key role in cancer progression. Indeed, the activation of lymphatic endothelial cells (LECs) through the lymphangiogenic process allows for the formation of new lymphatic vessels (LVs) that represent the major route for the dissemination of solid tumors. This process is governed by a plethora of cancer-derived and microevironmental mediators that strictly activate and control specific molecular pathways in LECs. In this work we used an in vitro model of LEC activation to trigger lymphangiogenesis using a mix of recombinant pro-lymphangiogenic factors (VFS) and a co-culture system with human melanoma cells. Both systems efficiently activated LECs, and under these experimental conditions, RNA sequencing was exploited to unveil the transcriptional profile of activated LECs. Our data demonstrate that both recombinant and tumor cell-mediated activation trigger significant molecular pathways associated with endothelial activation, morphogenesis, and cytokine-mediated signaling. In addition, this system provides information on new genes to be further investigated in the lymphangiogenesis process and open the possibility for further exploitation in other tumor contexts where lymphatic dissemination plays a relevant role

Dispersão de machos estéreis de Ceratitis capitata (Wied.) em pomar comercial de manga (Mangifera indica L.) na região Sudoeste da Bahia.

O cultivo de manga é a principal opção econômica do pólo de fruticultura de Anagé, Região Sudoeste da BA. As moscas-das-frutas são pragas quarentenárias e se constituem nos principais problemas fitossanitários da região. Em conformidade com os princípios da Produção Integrada de Frutas, a Técnica do Inseto Estéril (TIE) apresenta-se como uma estratégia de manejo vantajosa e de baixo impacto ambiental. Estudos sobre dispersão de moscamed (Ceratitis capitata Wied.) estéril são necessários para gerar subsídios à implantação da TIE no Brasil. Esse trabalho teve por objetivos estimar a capacidade de dispersão de machos moscamed estéril em pomar comercial de manga e a sua sobrevivência em campo. Cerca de 18.000 machos estéreis de moscamed, linhagem TSL Viena 8, procedentes de Mendonza, Argentina, foram liberados no centro do pomar de 20 ha, às 06h00. Para a recaptura, foram utilizadas armadilhas Jackson, dispostas a cada 25m nas direções N, S, L, O, NO, NE. SO e SE, totalizando 80 armadilhas, num raio máximo de 250 m. No 1º, 3º, 5º, 7º, 9º e 11º dia da liberação, as armadilhas foram expostas às 8h00 e retiradas após uma hora, sendo levadas a uma sala escura, sob lâmpada negra, para contagem de machos estéreis e selvagens. Foram recapturados apenas 22 machos estéreis (0,12%) em 16 armadilhas (20,0%) no primeiro dia após a liberação, sendo que nos demais dias a recaptura foi nula. As recapturas ocorreram principalmente nas armadilhas a sudoeste do ponto de liberação, à distância mínima de 25 metros (SO, NE, NO, SE) e máxima de 250 metros (S). Boa parte dos adultos (40%) foi recapturada num raio de 50m do ponto de liberação. A distância média de dispersão foi de 157,0 m/dia e a área abrangida foi de 29.008,33 m²/dia. A sobrevivência dos machos em campo foi insatisfatóri

Efeito de lodo de esgoto na fertilidade de um argissolo vermelho-amarelo cultivado com cana-de-açúcar.

O objetivo deste trabalho foi avaliar os efeitos da aplicação de 0, 20 e 40 Mg ha de lodo de esgoto, na presença e ausência de fertilizante mineral (NPK) na fertilidade e nos teores de metais pesados de um argissolo vermelho-amarelo distrófico, cultivado com cana-de-açúcar. Amostras de solo foram coletadas aos 146, 272 e 484 dias após o plantio da cultura de cana-de-açúcar e foram submetidas à análise de fertilidade pelo método do Instituto Agronômico de Campinas, incluindo a determinação do S, Cr, Cd, Cu, Fe, Mn, Pb e Zn. O lodo de esgoto diminuiu a acidez do solo e forneceu Ca, P, S e Zn, principalmente. Seus efeitos foram de curta duração, restringindo-se a um ano agrícola. os teores dos metais pesados das amostras de solo tratado foram maiores que os da testemunha, mas menores que os valores considerados perigosos ao ambiente

Standalone vertex finding in the ATLAS muon spectrometer

A dedicated reconstruction algorithm to find decay vertices in the ATLAS muon spectrometer is presented. The algorithm searches the region just upstream of or inside the muon spectrometer volume for multi-particle vertices that originate from the decay of particles with long decay paths. The performance of the algorithm is evaluated using both a sample of simulated Higgs boson events, in which the Higgs boson decays to long-lived neutral particles that in turn decay to bbar b final states, and pp collision data at √s = 7 TeV collected with the ATLAS detector at the LHC during 2011

Measurements of Higgs boson production and couplings in diboson final states with the ATLAS detector at the LHC

Measurements are presented of production properties and couplings of the recently discovered Higgs boson using the decays into boson pairs, H →γ γ, H → Z Z∗ →4l and H →W W∗ →lνlν. The results are based on the complete pp collision data sample recorded by the ATLAS experiment at the CERN Large Hadron Collider at centre-of-mass energies of √s = 7 TeV and √s = 8 TeV, corresponding to an integrated luminosity of about 25 fb−1. Evidence for Higgs boson production through vector-boson fusion is reported. Results of combined fits probing Higgs boson couplings to fermions and bosons, as well as anomalous contributions to loop-induced production and decay modes, are presented. All measurements are consistent with expectations for the Standard Model Higgs boson

Measurement of the top quark pair cross section with ATLAS in pp collisions at √s=7 TeV using final states with an electron or a muon and a hadronically decaying τ lepton

A measurement of the cross section of top quark pair production in proton-proton collisions recorded with the ATLAS detector at the Large Hadron Collider at a centre-of-mass energy of 7 TeV is reported. The data sample used corresponds to an integrated luminosity of 2.05 fb -1. Events with an isolated electron or muon and a τ lepton decaying hadronically are used. In addition, a large missing transverse momentum and two or more energetic jets are required. At least one of the jets must be identified as originating from a b quark. The measured cross section, σtt-=186±13(stat.)±20(syst.)±7(lumi.) pb, is in good agreement with the Standard Model prediction

Measurement of the top quark-pair production cross section with ATLAS in pp collisions at \sqrt{s}=7\TeV

A measurement of the production cross-section for top quark pairs(\ttbar) in $pp$ collisions at \sqrt{s}=7 \TeV is presented using data recorded with the ATLAS detector at the Large Hadron Collider. Events are selected in two different topologies: single lepton (electron $e$ or muon $\mu$) with large missing transverse energy and at least four jets, and dilepton ($ee$, $\mu\mu$ or $e\mu$) with large missing transverse energy and at least two jets. In a data sample of 2.9 pb-1, 37 candidate events are observed in the single-lepton topology and 9 events in the dilepton topology. The corresponding expected backgrounds from non-\ttbar Standard Model processes are estimated using data-driven methods and determined to be $12.2 \pm 3.9$ events and $2.5 \pm 0.6$ events, respectively. The kinematic properties of the selected events are consistent with SM \ttbar production. The inclusive top quark pair production cross-section is measured to be \sigmattbar=145 \pm 31 ^{+42}_{-27} pb where the first uncertainty is statistical and the second systematic. The measurement agrees with perturbative QCD calculations.Comment: 30 pages plus author list (50 pages total), 9 figures, 11 tables, CERN-PH number and final journal adde

Expected Performance of the ATLAS Experiment - Detector, Trigger and Physics

A detailed study is presented of the expected performance of the ATLAS detector. The reconstruction of tracks, leptons, photons, missing energy and jets is investigated, together with the performance of b-tagging and the trigger. The physics potential for a variety of interesting physics processes, within the Standard Model and beyond, is examined. The study comprises a series of notes based on simulations of the detector and physics processes, with particular emphasis given to the data expected from the first years of operation of the LHC at CERN

Hunt for new phenomena using large jet multiplicities and missing transverse momentum with ATLAS in 4.7 fb−1 of √s=7 TeV proton-proton collisions

Results are presented of a search for new particles decaying to large numbers of jets in association with missing transverse momentum, using 4.7 fb−1 of pp collision data at s√=7TeV collected by the ATLAS experiment at the Large Hadron Collider in 2011. The event selection requires missing transverse momentum, no isolated electrons or muons, and from ≥6 to ≥9 jets. No evidence is found for physics beyond the Standard Model. The results are interpreted in the context of a MSUGRA/CMSSM supersymmetric model, where, for large universal scalar mass m 0, gluino masses smaller than 840 GeV are excluded at the 95% confidence level, extending previously published limits. Within a simplified model containing only a gluino octet and a neutralino, gluino masses smaller than 870 GeV are similarly excluded for neutralino masses below 100 GeV