Canine distemper virus, atypical toxoplasma gondii, and neospora caninum co-infection, in a dog with neurological signs from Argentina

The occurrence of combined infections by Toxoplasma gondii, Neospora caninum and Canine distemper virus (CDV) in domestic dogs and wildlife animals has not been frequently reported, and the histopathological findings were not exhaustively described. The objective of this study was to report a co-infection of CDV, T. gondii and N. caninum in a dog with neurological signs, as well as the molecular characterization of the protozoa involved. A young street dog was rescued with neurological clinical signs and died spontaneously. A complete necropsy was performed. Tissues were collected and fixed for histopathological evaluation. Additionally, sections of the central nervous system (CNS) and heart were assayed by immunohistochemistry (IHQ) for T. gondii and N. caninum. Sample of brain tissue was analyzed by PCR and nPCR-RFLP for T. gondii genotyping. Spleen was used for detection of CDV by RT-PCR. Gross lesions were not observed, with the exception of the lung. Microscopically, a severe necrosuppurative meningoencephalitis with vasculitis, tachyzoites and bradyzoites of T. gondii and N. caninum were found. Demyelination was also evident, associated with eosinophilic intranuclear inclusion bodies within astrocytes. CDV was PCR positive while both parasites were presented PCR and IHQ positive results. Molecular characterization of T. gondii was reported as atypical #14 (likely). To our knowledge, this is the first report of genetical identification of T. gondii obtained from the brain of a naturally infected dog in Argentina. The results emphasize the importance of different techniques as diagnostic tools to enhance the detection of causative agents in cases of fatal encephalitis.Fil: Postma, Gabriela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Dellarupe, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Streitenberger, Nicolás. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Patología. Cátedra de Patología Especial Veterinaria; ArgentinaFil: Bratanich, Ana Cristina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Venturini, María Cecilia. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Epizootiología y Salud Pública. Laboratorio de Inmunoparasitología; ArgentinaFil: Minatel, Leonardo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentin

The experience of nurses as an accompanying family member

Objective: To describe the experience of nurses in the condition of the hospitalized patient's family companion. Methods: Qualitative study conducted with nine nurses working in a public and teaching hospital, in the South of Brazil. The data was produced by Dynamics of Creativity and Sensitivity and analyzed by the presuppositions of discourse analysis in its French chain. Results: From the analysis of the data produced the following themes emerged: the professional knowledge as intervening factor: from frustration to confrontation; being a nurse, being in the accompanying condition and decision making and; the challenges of nursing in the accompanying condition. Conclusion: The experience as an accompanying family member had repercussions on the "professional self" and the "family self", provoking considerations both in the professional life and in the nurses' personal life, which triggered reflections and modifications in their praxis

A vivência de enfermeiras na condição de familiar acompanhante The experience of nurses as an accompanying family member

Objetivo: Descrever a vivência de enfermeiros na condição de familiar acompanhante de paciente hospitalizado. Métodos: Estudo qualitativo realizado com nove enfermeiras que atuam em um hospital público e de ensino, na região Sul, do Brasil. Os dados foram produzidos por meio de Dinâmicas de Criatividade e Sensibilidade e analisados seguindo os pressupostos da análise de discurso, em sua corrente francesa. Resultados: Da análise dos dados produzidos emergiram os temas: O conhecimento profissional como fator interveniente: da frustração ao enfrentamento; ser enfermeiro, estar na condição de acompanhante e a tomada de decisões; e os desafios do enfermeiro na condição de acompanhante. Conclusão: A vivência como familiar acompanhante repercutiu ambiguidades ao “eu profissional e o “eu familiar”, suscitando ponderações tanto na vida profissional quanto na pessoal das enfermeiras, o que desencadeou reflexões e modificações em suas práxis.

A vivência de enfermeiras na condição de familiar acompanhante The experience of nurses as an accompanying family member

Objetivo: Descrever a vivência de enfermeiros na condição de familiar acompanhante de paciente hospitalizado. Métodos: Estudo qualitativo realizado com nove enfermeiras que atuam em um hospital público e de ensino, na região Sul, do Brasil. Os dados foram produzidos por meio de Dinâmicas de Criatividade e Sensibilidade e analisados seguindo os pressupostos da análise de discurso, em sua corrente francesa. Resultados: Da análise dos dados produzidos emergiram os temas: O conhecimento profissional como fator interveniente: da frustração ao enfrentamento; ser enfermeiro, estar na condição de acompanhante e a tomada de decisões; e os desafios do enfermeiro na condição de acompanhante. Conclusão: A vivência como familiar acompanhante repercutiu ambiguidades ao “eu profissional e o “eu familiar”, suscitando ponderações tanto na vida profissional quanto na pessoal das enfermeiras, o que desencadeou reflexões e modificações em suas práxis.

Avaliação da fragilidade de idosos em tratamento quimioterápico ambulatorial

Aim: to characterize and verify the fragility of the elderly in outpatient chemotherapy treatment in a university hospital of southern Brazil, and to identify the association between fragility and the following variables: chemotherapeutic effect, treatment time and selfreported diseases. Method: cross-sectional study conducted with 70 elderly in the chemotherapy outpatient, from August to September 2014 by means of Edmonton Frail Scale and characterization form. Results: fifty percent did not present fragility, 31.4% were apparently vulnerable and 18.6% had some level of fragility. Among the frail elderly, 15.7% had mild fragility, 1.4% moderate and 1.4% severe. The association between frailty and self-reported diseases, time of treatment and chemotherapeutic effects was not statistically significant. Conclusion: it was point to applicability of the scale to identify predisposing factors for the fragility syndrome and to enable preventive actions for the elderly in chemotherapeutic treatmentObjetivo: caracterizar y verificar la fragilidad de los ancianos en tratamiento de quimioterapia ambulatoria, en un hospital universitario, en el sur de Brasil, e identificar la asociación entre la fragilidad y las variables de efecto quimioterapéutico, relacionadas al tiempo de tratamiento y la enfermedad auto reporte. Método: estudio transversal realizado con 70 ancianos de un ambulatorio de quimioterapia, entre agosto y septiembre de 2014, a través de la Edmonton Frail Scale y forma de caracterización. Resultados: 50% no presentaron fragilidad, 31,4% eran aparentemente vulnerables y 18,6% tenía algún nivel de fragilidad. Entre los ancianos frágiles 15,7% tenían una fragilidad leve, 1,4% moderada y 1,4% severa. La asociación entre fragilidad y enfermedades auto referidas, y tiempo de tratamiento y efectos quimioterápicos no presentó significancia estadística. Conclusión: se observó la aplicabilidad de la escala para identificar factores predisponentes para el síndrome de fragilidad y viabilizar acciones preventivas a los ancianos en tratamiento quimioterápico.Objetivo: caracterizar e verificar a fragilidade de idosos em tratamento quimioterápico ambulatorial em um Hospital Universitário da região Sul do Brasil e, identificar a associação entre a fragilidade e as variáveis: efeito quimioterápico, tempo de tratamento e doenças autorreferidas. Método: estudo transversal desenvolvido com 70 idosos em um ambulatório de quimioterapia, de agosto a setembro de 2014, por meio da Edmonton Frail Scale e do formulário de caracterização. As análises estatísticas foram realizadas no programa Statistical Package for the Social Sciences versão 21. Resultados: houve predomínio de idosos do sexo masculino (61,4%), casados (62,8%), com ensino fundamental incompleto (71,4%), com renda mensal de até dois salários mínimos (82,8%) e a metade não apresentava fragilidade (50%). Conclusão: as variáveis analisadas não apresentaram significância, portanto não representam um fator de risco para a fragilidade de idosos em tratamento quimioterápico

Antigenic Proteins Involved in Occupational Rhinitis 1 and Asthma Caused by Obeche Wood (Triplochiton Scleroxylon)

Background Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. Objective To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma Materials and methods An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. Results Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA−), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases. Conclusions Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens

SARS-CoV-2 lineage B.1.1.7 is associated with greater disease severity among hospitalised women but not men: multicentre cohort study.

BACKGROUND: SARS-CoV-2 lineage B.1.1.7 has been associated with an increased rate of transmission and disease severity among subjects testing positive in the community. Its impact on hospitalised patients is less well documented. METHODS: We collected viral sequences and clinical data of patients admitted with SARS-CoV-2 and hospital-onset COVID-19 infections (HOCIs), sampled 16 November 2020 to 10 January 2021, from eight hospitals participating in the COG-UK-HOCI study. Associations between the variant and the outcomes of all-cause mortality and intensive therapy unit (ITU) admission were evaluated using mixed effects Cox models adjusted by age, sex, comorbidities, care home residence, pregnancy and ethnicity. FINDINGS: Sequences were obtained from 2341 inpatients (HOCI cases=786) and analysis of clinical outcomes was carried out in 2147 inpatients with all data available. The HR for mortality of B.1.1.7 compared with other lineages was 1.01 (95% CI 0.79 to 1.28, p=0.94) and for ITU admission was 1.01 (95% CI 0.75 to 1.37, p=0.96). Analysis of sex-specific effects of B.1.1.7 identified increased risk of mortality (HR 1.30, 95% CI 0.95 to 1.78, p=0.096) and ITU admission (HR 1.82, 95% CI 1.15 to 2.90, p=0.011) in females infected with the variant but not males (mortality HR 0.82, 95% CI 0.61 to 1.10, p=0.177; ITU HR 0.74, 95% CI 0.52 to 1.04, p=0.086). INTERPRETATION: In common with smaller studies of patients hospitalised with SARS-CoV-2, we did not find an overall increase in mortality or ITU admission associated with B.1.1.7 compared with other lineages. However, women with B.1.1.7 may be at an increased risk of admission to intensive care and at modestly increased risk of mortality.This report was produced by members of the COG-UK-HOCI Variant substudy consortium. COG-UK-HOCI is part of COG-UK. COG-UK is supported by funding from the Medical Research Council (MRC) part of UK Research & Innovation (UKRI), the National Institute of Health Research (NIHR) and Genome Research Limited, operating as the Wellcome Sanger Institute

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Proyecto, investigación e innovación en urbanismo, arquitectura y diseño industrial

Actas de congresoLas VII Jornadas de Investigación “Encuentro y Reflexión” y I Jornadas de Investigación de becarios y doctorandos. Proyecto, investigación e innovación en Urbanismo, Arquitectura y Diseño Industrial se centraron en cuatro ejes: el proyecto; la dimensión tecnológica y la gestión; la dimensión social y cultural y la enseñanza en Arquitectura, Urbanismo y Diseño Industrial, sustentados en las líneas prioritarias de investigación definidas epistemológicamente en el Consejo Asesor de Ciencia y Tecnología de esta Universidad Nacional de Córdoba. Con el objetivo de afianzar continuidad, formación y transferencia de métodos, metodología y recursos se incorporó becarios y doctorandos de los Institutos de investigación. La Comisión Honoraria la integraron las tres Secretarias de Investigación de la Facultad, arquitectas Marta Polo, quien fundó y María del Carmen Franchello y Nora Gutiérrez Crespo quienes continuaron la tradición de la buena práctica del debate en la cotidianeidad de la propia Facultad. Los textos que conforman las VII Jornadas son los avances y resultados de las investigaciones realizadas en el bienio 2016-2018.Fil: Novello, María Alejandra. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Repiso, Luciana. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Mir, Guillermo. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Brizuela, Natalia. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Herrera, Fernanda. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Períes, Lucas. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Romo, Claudia. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Gordillo, Natalia. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; ArgentinaFil: Andrade, Elena Beatriz. Universidad Nacional de Córdoba. Facultad de Arquitectura, Urbanismo y Diseño; Argentin