Detection and identification of medically important microorganisms isolated from pigeon excreta collected in a university in a newly industrialized country

Exposure to animal feces contaminated with pathogenic microorganisms poses a risk to human health. The presence of fungi and yeasts in environments is related to adverse effects on respiratory health. An objective approach to mitigating possible health effects due to exposure to fungi and yeasts in closed environments is difficult due to the lack of guidelines. The aim of this work was, to identify and characterize microorganisms of medical importance isolated from pigeon feces collected on roof tiles of a university building and also to evaluate the hygienic-sanitary conditions of the study area. Excrement sampling points were established, and the microorganisms were identified by standard biochemical and microbiological methods followed by MALDI-TOF proteomics for identification at the species level. Microbial identification revealed the presence of medically important microorganisms in the samples analyzed, namely the fungi Candida krusei, Candida rugosa, Aspergillus spp., Cryptococcus neoformans and Trichosporon asahii and bacterium Klebsiela pneumoniae. The results obtained demonstrated the need for the development of methods that help mitigate risks to human health due to exposure to pathogenic agents in environments such as that studied

Aspectos anatomopatológicos das neoplasias malignas renais: Anatomopathological aspects of malignant renal neoplasms

As neoplasias renais correspondem ao crescimento exacerbado de células tumorais no interior dos rins, classificadas como benignas ou malignas. Neste estudo será abordado sobre as neoplasias malignas renais, a qual correspondem a maior prevalência e são representadas pelo carcinoma de células renais e o tumor de Wilms, com a finalidade de descrever a respeito dos aspectos anatomopatológicos, disseminando informações para o diagnóstico e manejo precoce. O carcinoma de células renais é mais prevalente no sexo masculino, indivíduos mais velhos, geralmente assintomático, contribuindo para o diagnóstico tardio junto a existência de metástases e terapêutica irresponsiva. Não se trata de uma doença genética, sendo o caráter esporádico o predominante, neste contexto os fatores de risco, sobretudo o tabagismo em seguida de obesidade hemodiálise e doenças genéticas são potenciais desencadeantes da enfermidade. Os exames complementares associado a clínica, junto ao acompanhamento eleva a possibilidade de identificação antes de avanços metastáticos. O tumor de Wilms é típico de crianças, acometendo um ou ambos os rins, normalmente com alguma anomalia genética, sendo os sinais inespecíficos, mas sempre manifestando massa palpável e dor abdominal, a qual os métodos de imagem confirmam o diagnóstico e estimam o prognóstico deste. Neste contexto, elucida-se a transcendência que os aspectos anatomopatológicos das neoplasias malignas renais oferecem para a diagnose precoce, devido a escassez e inespecificidafe das manifestações clínicas. Logo, a junção do perfil de cada neoplasia abordado conduz ao manejo adequado e reduz a incidência de tratamentos agressivos e irresponsivos

Polymorphisms in the MBL2 gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19

IntroductionMannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19.MethodsBlood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PCR genotyping. Plasma measurements of MBL and cytokines were performed by enzyme-linked immunosorbent assay and flow cytometry, respectively.ResultsThe frequencies of the polymorphic MBL2 genotype (OO) and allele (O) were higher in patients with severe COVID-19 (p< 0.05). The polymorphic genotypes (AO and OO) were associated with lower MBL levels (p< 0.05). IL-6 and TNF-α were higher in patients with low MBL and severe COVID-19 (p< 0.05). No association of polymorphisms, MBL levels, or cytokine levels with long COVID was observed.DiscussionThe results suggest that, besides MBL2 polymorphisms promoting a reduction in MBL levels and therefore in its function, they may also contribute to the development of a more intense inflammatory process responsible for the severity of COVID-19

Measurement of nuclear modification factors of gamma(1S)), gamma(2S), and gamma(3S) mesons in PbPb collisions at root s(NN)=5.02 TeV

The cross sections for ϒ(1S), ϒ(2S), and ϒ(3S) production in lead-lead (PbPb) and proton-proton (pp) collisions at √sNN = 5.02 TeV have been measured using the CMS detector at the LHC. The nuclear modification factors, RAA, derived from the PbPb-to-pp ratio of yields for each state, are studied as functions of meson rapidity and transverse momentum, as well as PbPb collision centrality. The yields of all three states are found to be significantly suppressed, and compatible with a sequential ordering of the suppression, RAA(ϒ(1S)) > RAA(ϒ(2S)) > RAA(ϒ(3S)). The suppression of ϒ(1S) is larger than that seen at √sNN = 2.76 TeV, although the two are compatible within uncertainties. The upper limit on the RAA of ϒ(3S) integrated over pT, rapidity and centrality is 0.096 at 95% confidence level, which is the strongest suppression observed for a quarkonium state in heavy ion collisions to date. © 2019 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Funded by SCOAP3.Peer reviewe

Electroweak production of two jets in association with a Z boson in proton-proton collisions root s =13 TeV

A measurement of the electroweak (EW) production of two jets in association with a Z boson in proton-proton collisions at root s = 13 TeV is presented, based on data recorded in 2016 by the CMS experiment at the LHC corresponding to an integrated luminosity of 35.9 fb(-1). The measurement is performed in the lljj final state with l including electrons and muons, and the jets j corresponding to the quarks produced in the hard interaction. The measured cross section in a kinematic region defined by invariant masses m(ll) > 50 GeV, m(jj) > 120 GeV, and transverse momenta P-Tj > 25 GeV is sigma(EW) (lljj) = 534 +/- 20 (stat) fb (syst) fb, in agreement with leading-order standard model predictions. The final state is also used to perform a search for anomalous trilinear gauge couplings. No evidence is found and limits on anomalous trilinear gauge couplings associated with dimension-six operators are given in the framework of an effective field theory. The corresponding 95% confidence level intervals are -2.6 <cwww/Lambda(2) <2.6 TeV-2 and -8.4 <cw/Lambda(2) <10.1 TeV-2. The additional jet activity of events in a signal-enriched region is also studied, and the measurements are in agreement with predictions.Peer reviewe

Measurement of the Splitting Function in &ITpp &ITand Pb-Pb Collisions at root&ITsNN&IT=5.02 TeV

Data from heavy ion collisions suggest that the evolution of a parton shower is modified by interactions with the color charges in the dense partonic medium created in these collisions, but it is not known where in the shower evolution the modifications occur. The momentum ratio of the two leading partons, resolved as subjets, provides information about the parton shower evolution. This substructure observable, known as the splitting function, reflects the process of a parton splitting into two other partons and has been measured for jets with transverse momentum between 140 and 500 GeV, in pp and PbPb collisions at a center-of-mass energy of 5.02 TeV per nucleon pair. In central PbPb collisions, the splitting function indicates a more unbalanced momentum ratio, compared to peripheral PbPb and pp collisions.. The measurements are compared to various predictions from event generators and analytical calculations.Peer reviewe

Azimuthal separation in nearly back-to-back jet topologies in inclusive 2-and 3-jet events in pp collisions at root s=13TeV

A measurement for inclusive 2- and 3-jet events of the azimuthal correlation between the two jets with the largest transverse momenta, Delta phi(12), is presented. The measurement considers events where the two leading jets are nearly collinear ("back-to-back") in the transverse plane and is performed for several ranges of the leading jet transverse momentum. Proton-proton collision data collected with the CMS experiment at a center-of-mass energy of 13 TeV and corresponding to an integrated luminosity of 35.9 fb(-1) are used. Predictions based on calculations using matrix elements at leading-order and next-to-leading-order accuracy in perturbative quantum chromodynamics supplemented with leading-log parton showers and hadronization are generally in agreement with themeasurements. Discrepancies between the measurement and theoretical predictions are as large as 15%, mainly in the region 177 degrees <Delta phi(12) <180 degrees. The 2- and 3-jet measurements are not simultaneously described by any of models.Peer reviewe