Liittymätyypin muuttaminen kiertoliittymäksi ja sen vaikutuksia

Opinnäytetyön tarkoituksena oli tutkia ja selvittää liittymätyypin muutoksen vaikutuksia, kun se muutetaan kiertoliittymäksi. Työssä käytiin läpi kolme kiertoliittymää, jotka ovat olleet ennen muutosta valo-ohjaamattomia tai -ohjattuja tasoliittymiä. Liittymät sijaitsevat Lappeenrannassa, työn tilaajana toimi Lappeenrannan kaupunki ja työn ohjaavana opettajana Hämeen ammattikorkeakoulusta Janne Rautio. Liittymiä on muutettu ja tullaan muuttamaan yhä enemmän kiertoliittymiksi. Sille haettiin perusteluita tutkimuksen kautta mm. liikennemääriä, onnettomuustilastoja ja kustannuksia selvittämällä. Kaupungin tietokannoista löytyi asiasta paljon dataa. Niistä sain työtäni koskevien liittymien tilastot, joista keräsin tiedot työhöni. Lisäksi tutkin ja suoritin maastokäyntejä itse saadakseni ajantasaista tietoa liittymistä. Liittymien muuttamisella kiertoliittymiksi on paljon positiivisia ja melko vähän negatiivisia vaikutuksia. Se ei sovi jokaiseen paikkaan tilantarpeen vuoksi, mutta hyötyinä ovat liikenneturvallisuuden ja sujuvuuden parantuminen, rakennus- ja huoltokustannukset ovat pienemmät ja lisäksi onnettomuuksien vähenemisen myötä niistä aiheutuvat kustannukset pienenevät huomattavasti.Purpose of this thesis was to investigate and clarify effects of changing the intersection to a roundabout. Thesis contains three roundabouts, that have been before change either intersections with traffic lights, or without them. Intersections are located in Lappeenranta, thesis was ordered by city of Lappeenranta and Janne Rautio was director from Häme University of applied sciences. Intersections have been changed and will be changed much more in the future to roundabouts. For that I tried to get arguments with investigation about traffic quantity, statistics of accidents and costs. Lots of useful data was found from database of the city of Lappeenranta. From there I got statistics and information about intersections that are included in this thesis. Also I investigated and visited intersections to collect up-to-date information and data. Changing intersection’s type to roundabouts has much positive and just few negative effects. Roundabout needs a lot of space, so it doesn’t fit to every place, but as benefits there are improved traffic safety and fluency, build- and maintenance costs are smaller and also because number of accidents are smaller, costs from them are much smaller also

Distinct and Overlapping Brain Areas Engaged during Value-Based, Mathematical, and Emotional Decision Processing.

When comparing between the values of different choices, human beings can rely on either more cognitive processes, such as using mathematical computation, or more affective processes, such as using emotion. However, the neural correlates of how these two types of processes operate during value-based decision-making remain unclear. In this study, we investigated the extent to which neural regions engaged during value-based decision-making overlap with those engaged during mathematical and emotional processing in a within-subject manner. In a functional magnetic resonance imaging experiment, participants viewed stimuli that always consisted of numbers and emotional faces that depicted two choices. Across tasks, participants decided between the two choices based on the expected value of the numbers, a mathematical result of the numbers, or the emotional face stimuli. We found that all three tasks commonly involved various cortical areas including frontal, parietal, motor, somatosensory, and visual regions. Critically, the mathematical task shared common areas with the value but not emotion task in bilateral striatum. Although the emotion task overlapped with the value task in parietal, motor, and sensory areas, the mathematical task also evoked responses in other areas within these same cortical structures. Minimal areas were uniquely engaged for the value task apart from the other two tasks. The emotion task elicited a more expansive area of neural activity whereas value and mathematical task responses were in more focal regions. Whole-brain spatial correlation analysis showed that valuative processing engaged functional brain responses more similarly to mathematical processing than emotional processing. While decisions on expected value entail both mathematical and emotional processing regions, mathematical processes have a more prominent contribution particularly in subcortical processes

Na +/H + Antiport Is Essential for Yersinia pestis Virulence

Na⁺/H⁺ antiporters are ubiquitous membrane proteins that play a central role in the ion homeostasis of cells. In this study, we examined the possible role of Na⁺/H⁺ antiport in Yersinia pestis virulence and found that Y. pestis strains lacking the major Na⁺/H⁺ antiporters, NhaA and NhaB, are completely attenuated in an in vivo model of plague. The Y. pestis derivative strain lacking the nhaA and nhaB genes showed markedly decreased survival in blood and blood serum ex vivo. Complementation of either nhaA or nhaB in trans restored the survival of the Y. pestis nhaA nhaB double deletion mutant in blood. The nhaA nhaB double deletion mutant also showed inhibited growth in an artificial serum medium, Opti-MEM, and a rich LB-based medium with Na⁺ levels and pH values similar to those for blood. Taken together, these data strongly suggest that intact Na⁺/H⁺ antiport is indispensable for the survival of Y. pestis in the bloodstreams of infected animals and thus might be regarded as a promising noncanonical drug target for infections caused by Y. pestis and possibly for those caused by other blood-borne bacterial pathogens.Keywords: Vibrio cholerae, Inhibitors, Expression, Ph, Escherichia coli, nhaB, Low calcium response, Homeostasis, Bacteria, resistanc

Proceso agrario en Bolivia y América Latina

El material esta distribuido en cinco partes. La primera está referida a los resultados y perspectivas de la Reforma Agraria en Bolivia. Está dividida en tres temáticas: la Reforma Agraria en las Tierras Altas, la Reforma Agraria en las Tierras Bajas y la Reforma Agraria en el Chaco. La segunda parte contiene las intervenciones realizadas por los invitados extranjeros sobre los resultados y perspectivas de la Reforma Agraria en América Latina y en México, Perú y Ecuador. La tercera parte corresponde a las intervenciones de los representantes de las organizaciones indígenas y campesinas bolivianas, que fueron invitados para debatir el tema Tierra, Democracia y Poder. Esta parte esta precedida por un recuento general de la situación de los Movimientos Indígenas-Campesinos en América Latina y sus perspectivas. La cuarta da cuenta de las intervenciones hechas por los representantes de las organizaciones de productores agrarios, campesinos y empresarios, quienes fueron invitados para debatir el tema Tierra, Economía e Integración. La última parte es una síntesis de las ideas y aspectos relevantes producto de las exposiciones y de las preguntas de los asistentes al seminario, que dejaron una estela de inquietudes e iniciativas que son el eslabón para futuros debates en otros espacios de reflexión en diferentes lugares del país, con la participación de actores y pensadores, operadores y promotores del desarrollo rural.Presentación 7; Introducción 8; La Reforma Agraria abandonada: valles y altiplano, Miguel Urioste F. de C. 19; Medio siglo de Reforma Agraria boliviana, Danilo Paz Ballivián 53; La tierra es de quien la trabaja, René Salomón Vargas 65; La Reforma Agraria en las tierras bajas de Bolivia, Carlos Romero Bonifaz 83; Lógicas de ocupación territorial en la agricultura y la forestería empresarial en las tierras bajas, Alan Bojanic 127; Antecedentes, situación actual y recomendaciones en la tenencia de la tierra en el oriente boliviano, Cámara Agropecuaria del Oriente 137; 50 años de la Reforma Agraria en el Chaco boliviano, Oscar Bazoberry Chali 145; Resultados de los cambios introducidos por la Reforma Agraria, Erwin Galoppo von Borries 177; La Reforma Agraria en Bolivia y el MST, Ermelinda Fernández Bamba 191; La Reforma Agraria en América Latina, Jacques Chonchol 205; De rústicas revueltas: Añoranza y utopía en el México rural, Armando Bartra 223; Reforma y contrarreforma agraria en el Perú, Laureano del Castillo Pinto 255; Reforma Agraria en el Ecuador, Fausto Jordán B. 285; 50 años después, emergencia étnica, Xavier Albó 321; Tierra, mujer y desarrollo, María Machaca 329; Tierra y territorio comunitario, Bienvenido Zacu 333; Tierra, migración y colonización, José Luis Méndez Chaurara 341; Tierra, coca y desarrollo, Dionisio Núñez 355; Tierra para todos, Ángel Durán 359; Tierra y producción orgánica, Mario R. Cordero Camacho 367; Tierra y producción campesina, María Julia Jiménez 377; Tierra y Territorio y Políticas para la Tierra, Elizabeth Jiménez Zamora 385; Ideas y aspectos relevantes del Seminario, John D.Vargas Vega 429

Neural processes mediating contextual influences on human choice behaviour

Contextual influences on choice are ubiquitous in ecological settings. Current evidence suggests that subjective values are normalized with respect to the distribution of potentially available rewards. However, how this context-sensitivity is realised in the brain remains unknown. To address this, here we examine functional magnetic resonance imaging (fMRI) data during performance of a gambling task where blocks comprise values drawn from one of two different, but partially overlapping, reward distributions or contexts. At the beginning of each block (when information about context is provided), hippocampus is activated and this response is enhanced when contextual influence on choice increases. In addition, response to value in ventral tegmental area/substantia nigra (VTA/SN) shows context-sensitivity, an effect enhanced with an increased contextual influence on choice. Finally, greater response in hippocampus at block start is associated with enhanced context sensitivity in VTA/SN. These findings suggest that context-sensitive choice is driven by a brain circuit involving hippocampus and dopaminergic midbrain

Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.

The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens

The Neural Representation of Prospective Choice during Spatial Planning and Decisions

We are remarkably adept at inferring the consequences of our actions, yet the neuronal mechanisms that allow us to plan a sequence of novel choices remain unclear. We used functional magnetic resonance imaging (fMRI) to investigate how the human brain plans the shortest path to a goal in novel mazes with one (shallow maze) or two (deep maze) choice points. We observed two distinct anterior prefrontal responses to demanding choices at the second choice point: one in rostrodorsal medial prefrontal cortex (rd-mPFC)/superior frontal gyrus (SFG) that was also sensitive to (deactivated by) demanding initial choices and another in lateral frontopolar cortex (lFPC), which was only engaged by demanding choices at the second choice point. Furthermore, we identified hippocampal responses during planning that correlated with subsequent choice accuracy and response time, particularly in mazes affording sequential choices. Psychophysiological interaction (PPI) analyses showed that coupling between the hippocampus and rd-mPFC increases during sequential (deep versus shallow) planning and is higher before correct versus incorrect choices. In short, using a naturalistic spatial planning paradigm, we reveal how the human brain represents sequential choices during planning without extensive training. Our data highlight a network centred on the cortical midline and hippocampus that allows us to make prospective choices while maintaining initial choices during planning in novel environments

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

Is diet partly responsible for differences in COVID-19 death rates between and within countries?

Correction: Volume: 10 Issue: 1 Article Number: 44 DOI: 10.1186/s13601-020-00351-w Published: OCT 26 2020Reported COVID-19 deaths in Germany are relatively low as compared to many European countries. Among the several explanations proposed, an early and large testing of the population was put forward. Most current debates on COVID-19 focus on the differences among countries, but little attention has been given to regional differences and diet. The low-death rate European countries (e.g. Austria, Baltic States, Czech Republic, Finland, Norway, Poland, Slovakia) have used different quarantine and/or confinement times and methods and none have performed as many early tests as Germany. Among other factors that may be significant are the dietary habits. It seems that some foods largely used in these countries may reduce angiotensin-converting enzyme activity or are anti-oxidants. Among the many possible areas of research, it might be important to understand diet and angiotensin-converting enzyme-2 (ACE2) levels in populations with different COVID-19 death rates since dietary interventions may be of great benefit.Peer reviewe