225 research outputs found
Interface modiο¬cation of clay and graphene platelets reinforced epoxy nanocomposites: a comparative study
The interface between the matrix phase and dispersed phase of a composite plays a critical role in inο¬uencing its properties. However, the intricate mecha-nisms of interface are not fully understood, and polymer nanocomposites are no exception. This study compares the fabrication, morphology, and mechanical and thermal properties of epoxy nanocomposites tuned by clay layers (denoted as m-clay) and graphene platelets (denoted as m-GP). It was found that a chemical modiο¬cation, layer expansion and dispersion of ο¬ller within the epoxy matrix resulted in an improved interface between the ο¬ller mate-rial and epoxy matrix. This was conο¬rmed by Fourier transform infrared spectroscopy and transmission electron microscope. The enhanced interface led to improved mechanical properties (i.e. stiffness modulus, fracture toughness) and higher glass transition temperatures (Tg) compared with neat epoxy. At 4 wt% m-GP, the critical strain energy release rate G1c of neat epoxy improved by 240 % from 179.1 to 608.6 J/m2 and Tg increased from 93.7 to 106.4 οΏ½C. In contrast to m-clay, which at 4 wt%, only improved the G1c by 45 % and Tg by 7.1 %. The higher level of improvement offered by m-GP is attributed to the strong interaction of graphene sheets with epoxy because the covalent bonds between the carbon atoms of graphene sheets are much stronger than silicon-based clay
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Superiority of Dynamic Weights against Fixed Weights in Merging Multi-Satellite Precipitation Datasets over Pakistan
Data Availability Statement:
The data utilized in this study were acquired through purchase from the Pakistan Meteorology Department (PMD) and the Water and Power Development Authority. Information about the data is available at https://www.pmd.gov.pk/en/ (accessed on 1 January 2023) and http://www.wapda.gov.pk/ (accessed on 1 January 2023), respectively.Satellite precipitation products (SPPs) are undeniably subject to uncertainty due to retrieval algorithms and sampling issues. Many research efforts have concentrated on merging SPPs to create high-quality merged precipitation datasets (MPDs) in order to reduce these uncertainties. This study investigates the efficacy of dynamically weighted MPDs in contrast to those using static weights. The analysis focuses on comparing MPDs generated using the βdynamic clustered Bayesian averaging (DCBA)β approach with those utilizing the βregional principal component analysis (RPCA)β under fixed-weight conditions. These MPDs were merged from SPPs and reanalysis precipitation data, including TRMM (Tropical Rainfall Measurement Mission) Multi-satellite Precipitation Analysis (TMPA) 3B42V7, PERSIANN-CDR, CMORPH, and the ERA-Interim reanalysis precipitation data. The performance of these datasets was evaluated in Pakistanβs diverse climatic zonesβglacial, humid, arid, and hyper-aridβemploying data from 102 rain gauge stations. The effectiveness of the DCBA model was quantified using Theilβs U statistic, demonstrating its superiority over the RPCA model and other individual merging methods in the study area The comparative performances of DCBA and RPCA in these regions, as measured by Theilβs U, are 0.49 to 0.53, 0.38 to 0.45, 0.37 to 0.42, and 0.36 to 0.43 in glacial, humid, arid, and hyper-arid zones, respectively. The evaluation of DCBA and RPCA compared with SPPs at different elevations showed poorer performance at high altitudes (>4000 m). The comparison of MPDs with the best performance of SPP (i.e., TMPA) showed significant improvement of DCBA even at altitudes above 4000 m. The improvements are reported as 49.83% for mean absolute error (MAE), 42.31% for root-mean-square error (RMSE), 27.94% for correlation coefficient (CC), 40.15% for standard deviation (SD), and 13.21% for Theilβs U. Relatively smaller improvements are observed for RPCA at 13.04%, 1.56%, 10.91%, 1.67%, and 5.66% in the above indices, respectively. Overall, this study demonstrated the superiority of DCBA over RPCA with static weight. Therefore, it is strongly recommended to use dynamic variation of weights in the development of MPDs.National Natural Science Foundation of China (grant numbers 51839006 and 52250410336)
Recognizing true H5N1 infections in humans during confirmed outbreaks.
INTRODUCTION: The goal of this study was to evaluate whether any characteristics that are evident at presentation for urgent medical attention could be used to differentiate cases of H5N1 in the absence of viral testing. METHODOLOGY: Information about exposure to poultry, clinical signs and symptoms, treatments, and outcomes was abstracted from existing data in the global avian influenza registry (www.avianfluregistry.org) using standardized data collection tools for documented and possible cases of H5N1 infection who presented for medical attention between 2005-2011 during known H5N1 outbreaks in Azerbaijan, Indonesia, Pakistan and Turkey. RESULTS: Demography, exposure to poultry, and presenting symptoms were compared, with only the common symptoms of fever and headache presenting significantly more frequently in confirmed H5N1 cases than in possible cases. Reported exposure toΒ infected humans was also more common in confirmed cases. In contrast, unexplained respiratory illness, sore throat, excess sputum production, and rhinorrhea were more frequent in possible cases. Overall, oseltamivir treatment showed a survival benefit, with the greatest benefit shown in H5N1 cases who were treated within two days of symptom onset (51% reduction in case fatality). CONCLUSION: Since prompt treatment with antivirals conferred a strong survival benefit for H5N1 cases, presumptive antiviral treatment should be considered for all possible cases presenting during an outbreak of H5N1 as a potentially life-saving measure
A comprehensive 1000 Genomes-based genome-wide association meta-analysis of coronary artery disease
Existing knowledge of genetic variants affecting risk of coronary artery disease (CAD) is largely based on genome-wide association studies (GWAS) analysis of common SNPs. Leveraging phased haplotypes from the 1000 Genomes Project, we report a GWAS meta-analysis of 185 thousand CAD cases and controls, interrogating 6.7 million common (MAF>0.05) as well as 2.7 million low frequency (0.005<MAF<0.05) variants. In addition to confirmation of most known CAD loci, we identified 10 novel loci, eight additive and two recessive, that contain candidate genes that newly implicate biological processes in vessel walls. We observed intra-locus allelic heterogeneity but little evidence of low frequency variants with larger effects and no evidence of synthetic association. Our analysis provides a comprehensive survey of the fine genetic architecture of CAD showing that genetic susceptibility to this common disease is largely determined by common SNPs of small effect siz
Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR
<p>Abstract</p> <p>Background</p> <p>Species identification of isolates belonging to the <it>Mycobacterium tuberculosis </it>complex (MTC) seems to be important for the appropriate treatment of patients, since <it>M. bovis </it>is naturally resistant to a first line anti-tuberculosis (TB) drug, pyrazinamide, while most of the other MTC members are susceptible to this antimicrobial agent. A simple and low-cost differentiation method was needed in higher TB burden countries, such as Bangladesh, where the prevalence of <it>M. bovis </it>among people or cattle has not been investigated.</p> <p>Methods</p> <p>Genetic regions <it>cfp32</it>, RD9 and RD12 were chosen as targets for a species distinguishable multiplex PCR and the system was evaluated with twenty reference strains of mycobacterial species including non-tubercular mycobacteria (NTM). A total of 350 clinical MTC isolates obtained in Bangladesh were then analyzed with this multiplex PCR.</p> <p>Results</p> <p>All of the MTC reference strains gave expected banding patterns and no non-specific amplifications were observed in the NTM strains. Out of 350 clinical isolates examined by this method, 347 (99.1%) were positive for all of the <it>cfp32</it>, RD9 and RD12 and determined as <it>M. tuberculosis</it>. Two isolates lacked <it>cfp32 </it>PCR product and one lacked RD12, however, those three samples were further examined and identified as <it>M. tuberculosis </it>by the sequence analyses of <it>hsp65 </it>and <it>gyrB</it>.</p> <p>Conclusions</p> <p>The MTC-discrimination multiplex PCR (MTCD-MPCR) developed in this study showed high specificity and was thought to be very useful as a routine test because of its simplicity. In the current survey, all the 350 MTC isolates obtained from Bangladesh TB patients were determined as <it>M. tuberculosis </it>and no other MTC were detected. This result suggested the general TB treatment regimen including pyrazinamide to be the first choice in Bangladesh.</p
Measurement of Ο c1 and Ο c2 production with sβ = 7 TeV pp collisions at ATLAS
The prompt and non-prompt production cross-sections for the Ο c1 and Ο c2 charmonium states are measured in pp collisions at sβ = 7 TeV with the ATLAS detector at the LHC using 4.5 fbβ1 of integrated luminosity. The Ο c states are reconstructed through the radiative decay Ο c β J/ΟΞ³ (with J/Ο β ΞΌ + ΞΌ β) where photons are reconstructed from Ξ³ β e + e β conversions. The production rate of the Ο c2 state relative to the Ο c1 state is measured for prompt and non-prompt Ο c as a function of J/Ο transverse momentum. The prompt Ο c cross-sections are combined with existing measurements of prompt J/Ο production to derive the fraction of prompt J/Ο produced in feed-down from Ο c decays. The fractions of Ο c1 and Ο c2 produced in b-hadron decays are also measured
Topography of Extracellular Matrix Mediates Vascular Morphogenesis and Migration Speeds in Angiogenesis
The extracellular matrix plays a critical role in orchestrating the events necessary for wound healing, muscle repair, morphogenesis, new blood vessel growth, and cancer invasion. In this study, we investigate the influence of extracellular matrix topography on the coordination of multi-cellular interactions in the context of angiogenesis. To do this, we validate our spatio-temporal mathematical model of angiogenesis against empirical data, and within this framework, we vary the density of the matrix fibers to simulate different tissue environments and to explore the possibility of manipulating the extracellular matrix to achieve pro- and anti-angiogenic effects. The model predicts specific ranges of matrix fiber densities that maximize sprout extension speed, induce branching, or interrupt normal angiogenesis, which are independently confirmed by experiment. We then explore matrix fiber alignment as a key factor contributing to peak sprout velocities and in mediating cell shape and orientation. We also quantify the effects of proteolytic matrix degradation by the tip cell on sprout velocity and demonstrate that degradation promotes sprout growth at high matrix densities, but has an inhibitory effect at lower densities. Our results are discussed in the context of ECM targeted pro- and anti-angiogenic therapies that can be tested empirically
Interstitial cell migration: integrin-dependent and alternative adhesion mechanisms
Adhesion and migration are integrated cell functions that build, maintain and remodel the multicellular organism. In migrating cells, integrins are the main transmembrane receptors that provide dynamic interactions between extracellular ligands and actin cytoskeleton and signalling machineries. In parallel to integrins, other adhesion systems mediate adhesion and cytoskeletal coupling to the extracellular matrix (ECM). These include multifunctional cell surface receptors (syndecans and CD44) and discoidin domain receptors, which together coordinate ligand binding with direct or indirect cytoskeletal coupling and intracellular signalling. We review the way that the different adhesion systems for ECM components impact cell migration in two- and three-dimensional migration models. We further discuss the hierarchy of these concurrent adhesion systems, their specific tasks in cell migration and their contribution to migration in three-dimensional multi-ligand tissue environments
High and Low Molecular Weight Hyaluronic Acid Differentially Regulate Human Fibrocyte Differentiation
Following tissue injury, monocytes can enter the tissue and differentiate into fibroblast-like cells called fibrocytes, but little is known about what regulates this differentiation. Extracellular matrix contains high molecular weight hyaluronic acid (HMWHA; βΌ2Γ10(6) Da). During injury, HMWHA breaks down to low molecular weight hyaluronic acid (LMWHA; βΌ0.8-8Γ10(5) Da).In this report, we show that HMWHA potentiates the differentiation of human monocytes into fibrocytes, while LMWHA inhibits fibrocyte differentiation. Digestion of HMWHA with hyaluronidase produces small hyaluronic acid fragments, and these fragments inhibit fibrocyte differentiation. Monocytes internalize HMWHA and LMWHA equally well, suggesting that the opposing effects on fibrocyte differentiation are not due to differential internalization of HMWHA or LMWHA. Adding HMWHA to PBMC does not appear to affect the levels of the hyaluronic acid receptor CD44, whereas adding LMWHA decreases CD44 levels. The addition of anti-CD44 antibodies potentiates fibrocyte differentiation, suggesting that CD44 mediates at least some of the effect of hyaluronic acid on fibrocyte differentiation. The fibrocyte differentiation-inhibiting factor serum amyloid P (SAP) inhibits HMWHA-induced fibrocyte differentiation and potentiates LMWHA-induced inhibition. Conversely, LMWHA inhibits the ability of HMWHA, interleukin-4 (IL-4), or interleukin-13 (IL-13) to promote fibrocyte differentiation.We hypothesize that hyaluronic acid signals at least in part through CD44 to regulate fibrocyte differentiation, with a dominance hierarchy of SAP>LMWHAβ₯HMWHA>IL-4 or IL-13
Molecular Biomechanics: The Molecular Basis of How Forces Regulate Cellular Function
Recent advances have led to the emergence of molecular biomechanics as an essential element of modern biology. These efforts focus on theoretical and experimental studies of the mechanics of proteins and nucleic acids, and the understanding of the molecular mechanisms of stress transmission, mechanosensing and mechanotransduction in living cells. In particular, single-molecule biomechanics studies of proteins and DNA, and mechanochemical coupling in biomolecular motors have demonstrated the critical importance of molecular mechanics as a new frontier in bioengineering and life sciences. To stimulate a more systematic study of the basic issues in molecular biomechanics, and attract a broader range of researchers to enter this emerging field, here we discuss its significance and relevance, describe the important issues to be addressed and the most critical questions to be answered, summarize both experimental and theoretical/computational challenges, and identify some short-term and long-term goals for the field. The needs to train young researchers in molecular biomechanics with a broader knowledge base, and to bridge and integrate molecular, subcellular and cellular level studies of biomechanics are articulated.National Institutes of Health (U.S.) (grant UO1HL80711-05 to GB)National Institutes of Health (U.S.) (grant R01GM076689-01)National Institutes of Health (U.S.) (grant R01AR033236-26)National Institutes of Health (U.S.) (grant R01GM087677-01A1)National Institutes of Health (U.S.) (grant R01AI44902)National Institutes of Health (U.S.) (grant R01AI38282)National Science Foundation (U.S.) (grant CMMI-0645054)National Science Foundation (U.S.) (grant CBET-0829205)National Science Foundation (U.S.) (grant CAREER-0955291
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