A preliminary study: Does relationship closeness with grandchildren correlate with the quality of life and physical health of Malaysian Chinese elderly?

The 13th Next-Generation Global Workshop第13回次世代グローバルワークショップテーマ: New Risks and Resilience in Asian Societies and the World 日程: 21-23 November, 2020 開催場所: ベトナム社会科学院(ハノイ)/Vietnam Academy of Social Sciences(No. 1 Lieu Giai street, Ba Dinh, Hanoi, Vietnam) ※Due to the COVID-19, the workshop will be held at ONLINE for overseas participants(not from Vietnam)/ONSITE for Vietnamese participants.This preliminary mixed-method study aimed to investigate whether grandparental childcare can contribute to elderly' quality of life and physical health. In the quantitative study, a total of 97 Chinese grandparents who are primary caregivers were recruited to examine the relations of relationship closeness, quality of life and physical health. Correlational analysis revealed that grandparent-grandchildren relationship closeness positively correlated with self-rated quality of life and physical health of the grandparents. In the qualitative interview, grandmothers (n = four Chinese grandmothers) who are primary caregivers were recruited to share their experience in taking care of grandchildren. Findings from the thematic analysis revealed that all grandmothers have a close relationship with their grandchildren. In specific, they are happy with the companionship of grandchildren even though they may feel physically tired in taking care of grandchildren. This preliminary study on skipped generation family provides insights into understanding the contribution of relationship closeness with grandchildren on the perceived quality of life and physical health of Malaysian grandparents who are the primary caregiver to their grandchildren

Scanning the human proteome for calmodulin-binding proteins

The calcium ion (Ca(2+)) is a ubiquitous second messenger that is crucial for the regulation of a wide variety of cellular processes. The diverse transient signals transduced by Ca(2+) are mediated by intracellular Ca(2+)-binding proteins, also known as Ca(2+) sensors. A key obstacle to studying many Ca(2+)-sensing proteins is the difficulty in identifying the numerous downstream target interactions that respond to Ca(2+)-induced conformational changes. Among a number of Ca(2+) sensors in the eukaryotic cell, calmodulin (CaM) is the most widespread and the best studied. Employing the mRNA display technique, we have scanned the human proteome for CaM-binding proteins and have identified and characterized a large number of both known and previously uncharacterized proteins that interact with CaM in a Ca(2+)-dependent manner. The interactions of several identified proteins with Ca(2+)/CaM were confirmed by using pull-down assays and coimmunoprecipitation. Many of the CaM-binding proteins identified belong to protein families such as the DEAD/H box proteins, ribosomal proteins, proteasome 26S subunits, and deubiquitinating enzymes, suggesting the possible involvement of Ca(2+)/CaM in different signaling pathways. The selection method described herein could be used to identify the binding partners of other calcium sensors on the proteome-wide scale

Kinesin spindle protein (KSP) inhibitors in combination with chemotherapeutic agents for cancer therapy

A diverse group of proteins, the activities of which are precisely orchestrated during mitosis, have emerged as targets for cancer therapeutics; these include the Aurora kinases (AKs), Polo‐like kinases (PLKs), and the kinesin spindle protein (KSP). KSP is essential for the proper separation of spindle poles during mitosis. Agents that target KSP selectively act on cells undergoing cell division, which means that KSP inhibitors are mitosis‐specific drugs, and have demonstrated remarkable activities in vitro. However, a significant obstacle to the success of KSP inhibitors is that these compounds, with tremendous efficacy in vitro, have demonstrated little or even no antitumor activity in vivo. Accumulated data suggest that a combination of KSP inhibitors with various cytostatic drugs will result in a more powerful tumor‐killing effect than monotherapy. Combination therapies might predominate and represent the next frontier in the discovery research of KSP inhibitors as potential anticancer drugs. Few published studies have reviewed combination therapy using KSP inhibitors. Herein we provide a comprehensive review of the literature on KSP inhibitor monotherapy and therapeutic combinations. The current state and problems are also discussed. Two are better than one: Targeted therapies in the form of antibodies and small molecules have been studied in combination with kinesin spindle protein (KSP) inhibitors to enhance their efficacy against cancer in preclinical investigations. Accumulated data suggest that a combination of KSP inhibitors with various cytostatic drugs will result in a more powerful tumor‐killing effect than monotherapy. Combination therapies may predominate and represent the next frontier in discovery research for KSP inhibitors as potential anticancer drugs