974 research outputs found
On-site detection of equid alphaherpesvirus 3 in perineal and genital swabs of mares and stallions
Equine coital exanthema (ECE) is an infectious, venereally transmitted muco-cutaneous disease affecting mares and stallions, caused by equid alphaherpesvirus 3 (EHV3). Diagnostic tools for rapid identification of EHV3 are of primary importance to diminish the risk of EHV3 dissemination at the time of breeding. In the last years, it has been shown that the performance of the insulated-isothermal polymerase chain reaction (iiPCR) is comparable to virus isolation, nested PCR and real-time PCR (qPCR) in detecting pathogens of various animal species. Analytical sensitivity and specificity of the iiPCR were compared with a qPCR, using a plasmid containing the target region of the EHV3 glycoprotein G gene and an Argentinian EHV3 isolate (E/9283/07 C3A). In order to evaluate the diagnostic performance of the iiPCR, nucleic acids of 85 perineal and genital swabs (PGS) of mares and stallions were extracted by tacoTM mini and tested by both techniques. EHV3 was detected in 46 and 45 of the 85 PGS by the iiPCR and qPCR, respectively. There was almost perfect agreement between the two diagnostic methods (98.82%; 95% CI: 95.03â100%; Îș = 0.98). The iiPCR had a limit of detection of 95.00% at 6 genome equivalents per reaction and a detection endpoint for viral DNA comparable to that of the qPCR, and did not react with six non-targeted equine pathogens. The iiPCR represents a sensitive and specific method for the rapid on-site diagnosis of EHV3 infection. Its routinely implementation in breeding facilities, and artificial insemination and embryo transfer centers, will contribute to prevent the dissemination of this venereal, highly contagious disease in horses.Fil: Vissani, MarĂa Aldana. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; Argentina. Instituto Nacional de TecnologĂa Agropecuaria; ArgentinaFil: Tordoya, Maria Silvia. Instituto Nacional de TecnologĂa Agropecuaria; ArgentinaFil: Tsai, Y.-L.. GeneReach; Estados UnidosFil: Lee, P.-Y.A.. GeneReach; Estados UnidosFil: Shen, Y.-H.. GeneReach; Estados UnidosFil: Lee, F.-C.. GeneReach; Estados UnidosFil: Wang, H.-T.T.. GeneReach; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de TecnologĂa Agropecuaria; Argentina. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas; ArgentinaFil: Barrandeguy, MarĂa Edith. Instituto Nacional de TecnologĂa Agropecuaria; Argentina. Universidad del Salvador. Escuela de Veterinaria; Argentin
Multiwavelength Study of M8.9/3B Solar Flare from AR NOAA 10960
We present a multi-wavelength analysis of a long duration white-light solar
flare (M8.9/3B) event that occurred on 4 June 2007 from NOAA AR 10960. The
flare was observed by several spaceborne instruments, namely SOHO/MDI,
Hinode/SOT, TRACE and STEREO/SECCHI. The flare was initiated near a small,
positive-polarity, satellite sunspot at the centre of the AR, surrounded by
opposite-polarity field regions. MDI images of the AR show considerable amount
of changes in a small positive-polarity sunspot of delta configuration during
the flare event. SOT/G-band (4305 A) images of the sunspot also suggest the
rapid evolution of the positive-polarity sunspot with highly twisted penumbral
filaments before the flare event, which were oriented in the counterclockwise
direction. It shows the change in orientation and also remarkable disappearance
of twisted penumbral filaments (~35-40%) and enhancement in umbral area
(~45-50%) during the decay phase of the flare. TRACE and SECCHI observations
reveal the successive activations of two helical twisted structures associated
with this sunspot, and the corresponding brightening in the chromosphere as
observed by the time-sequence images of SOT/Ca II H line (3968 A). The
secondary-helical twisted structure is found to be associated with the M8.9
flare event. The brightening starts 6-7 min prior to the flare maximum with the
appearance of secondary helical-twisted structure. The flare intensity
maximizes as this structure moves away from the AR. This twisted flux-tube
associated with the flare triggering, is found to be failed in eruption. The
location of the flare is found to coincide with the activation site of the
helical twisted structures. We conclude that the activations of successive
helical twists in the magnetic flux tubes/ropes plays a crucial role in the
energy build-up process and triggering of M-class solar flare without a CME.Comment: 22 pages, 12 figures, Accepted for Publication in Solar Physic
The X10 Flare on 2003 October 29: Triggered by Magnetic Reconnection between Counter-Helical Fluxes?
Vector magnetograms taken at Huairou Solar Observing Station (HSOS) and Mees
Solar Observatory (MSO) reveal that the super active region (AR) NOAA 10486 was
a complex region containing current helicity flux of opposite signs. The main
positive sunspots were dominated by negative helicity fields, while positive
helicity patches persisted both inside and around the main positive sunspots.
Based on a comparison of two days of deduced current helicity density,
pronounced changes were noticed which were associated with the occurrence of an
X10 flare that peaked at 20:49 UT, 2003 October 29. The average current
helicity density (negative) of the main sunspots decreased significantly by
about 50. Accordingly, the helicity densities of counter-helical patches
(positive) were also found to decay by the same proportion or more. In
addition, two hard X-ray (HXR) `footpoints' were observed by the Reuven Ramaty
High Energy Solar Spectroscopic Imager (RHESSI} during the flare in the 50-100
keV energy range. The cores of these two HXR footpoints were adjacent to the
positions of two patches with positive current helicity which disappeared after
the flare. This strongly suggested that the X10 flare on 2003 Oct. 29 resulted
from reconnection between magnetic flux tubes having opposite current helicity.
Finally, the global decrease of current helicity in AR 10486 by ~50% can be
understood as the helicity launched away by the halo coronal mass ejection
(CME) associated with the X10 flare.Comment: Solar Physics, 2007, in pres
The critical amplitude ratio of the susceptibility in the random-site two-dimensional Ising model
We present a new way of probing the universality class of the site-diluted
two-dimensional Ising model. We analyse Monte Carlo data for the magnetic
susceptibility, introducing a new fitting procedure in the critical region
applicable even for a single sample with quenched disorder. This gives us the
possibility to fit simultaneously the critical exponent, the critical amplitude
and the sample dependent pseudo-critical temperature. The critical amplitude
ratio of the magnetic susceptibility is seen to be independent of the
concentration of the empty sites for all investigated values of . At the same time the average effective exponent is found
to vary with the concentration , which may be argued to be due to
logarithmic corrections to the power law of the pure system. This corrections
are canceled in the susceptibility amplitude ratio as predicted by theory. The
central charge of the corresponding field theory was computed and compared well
with the theoretical predictions.Comment: 6 pages, 4 figure
Quantum criticalities in a two-leg antiferromagnetic S=1/2 ladder induced by a staggered magnetic field
We study a two-leg antiferromagnetic spin-1/2 ladder in the presence of a
staggered magnetic field. We consider two parameter regimes: strong (weak)
coupling along the legs and weak (strong) coupling along the rungs. In both
cases, the staggered field drives the Haldane spin-liquid phase of the ladder
towards a Gaussian quantum criticality. In a generalized spin ladder with a
non-Haldane, spontaneously dimerized phase, the staggered magnetic field
induces an Ising quantum critical regime. In the vicinity of the critical
lines, we derive low-energy effective field theories and use these descriptions
to determine the dynamical response functions, the staggered spin
susceptibility and the string order parameter.Comment: 29 pages of revtex, 10 figure
Early Seizures Prematurely Unsilence Auditory Synapses to Disrupt Thalamocortical Critical Period Plasticity
Heightened neural excitability in infancy and childhood results in increased susceptibility to seizures. Such early-life seizures are associated with language deficits and autism that can result from aberrant development of the auditory cortex. Here, we show that early-life seizures disrupt a critical period (CP) for tonotopic map plasticity in primary auditory cortex (A1). We show that this CP is characterized by a prevalence of âsilent,â NMDA-receptor (NMDAR)-only, glutamate receptor synapses in auditory cortex that become âunsilencedâ due to activity-dependent AMPA receptor (AMPAR) insertion. Induction of seizures prior to this CP occludes tonotopic map plasticity by prematurely unsilencing NMDAR-only synapses. Further, brief treatment with the AMPAR antagonist NBQX following seizures, prior to the CP, pre
The inflammatory cytokine IL-6 induces FRA1 deacetylation promoting colorectal cancer stem-like properties
Colorectal cancer (CRC) has long been known for its tight association with chronic inflammation, thought to play a key role in tumor onset and malignant progression through the modulation of cancer stemness. However, the underlying molecular and cellular mechanisms are still largely elusive. Here we show that the IL-6/STAT3 inflammatory signaling axis induces the deacetylation of FRA1 at the Lys-116 residue located within its DNA-binding domain. The HDAC6 deacetylase underlies this key modification leading to the increase of FRA1 transcriptional activity, the subsequent transactivation of NANOG expression, and the acquisition of stem-like cellular features. As validated in a large (nâ=â123) CRC cohort, IL-6 secretion was invariably accompanied by increased FRA1 deacetylation at K116 and an overall increase in its protein levels, coincident with malignant progression and poor prognosis. Of note, combined treatment with the conventional cytotoxic drug 5-FU together with Tubastatin A, a HDAC6-specific inhibitor, resulted in a significant in vivo synergistic inhibitory effect on tumor growth through suppression of CRC stemness. Our results reveal a novel transcriptional and posttranslational regulatory cross-talk between inflammation and stemness signaling pathways that underlie self-renewal and maintenance of CRC stem cells and promote their malignant behavior. Combinatorial treatment aimed at the core regulatory mechanisms downstream of IL-6 may offer a novel promising approach for CRC treatment
Temporal evolution of solar energetic particle spectra
During solar flares and coronal mass ejections, solar energetic par- ticles (SEPs) may be released into the interplanetary medium and near-Earth locations. The energy spectra of SEP events at 1 AU are typically averaged over the entire event or studied in a few snapshots. In this paper we analyze the time evolution of the energy spectra of four large selected SEP events using a large number of snapshots. We use a multi-spacecraft and multi-instrument approach for the observations, obtained over a wide SEP energy range. We find large differences in the spectra at the beginning of the events as measured by different instruments. We show that over time, a wave-like structure is observed traveling through the spectra from the highest energies to the lowest energies, creating an âarchâ shape which then straightens into a power law later in the event, after times of the order of 10 hours. We discuss the processes that determine SEP intensities and their role in shaping the spectral time evolution
Intercalibration of the barrel electromagnetic calorimeter of the CMS experiment at start-up
Calibration of the relative response of the individual channels of the barrel electromagnetic calorimeter of the CMS detector was accomplished, before installation, with cosmic ray muons and test beams. One fourth of the calorimeter was exposed to a beam of high energy electrons and the relative calibration of the channels, the intercalibration, was found to be reproducible to a precision of about 0.3%. Additionally, data were collected with cosmic rays for the entire ECAL barrel during the commissioning phase. By comparing the intercalibration constants obtained with the electron beam data with those from the cosmic ray data, it is demonstrated that the latter provide an intercalibration precision of 1.5% over most of the barrel ECAL. The best intercalibration precision is expected to come from the analysis of events collected in situ during the LHC operation. Using data collected with both electrons and pion beams, several aspects of the intercalibration procedures based on electrons or neutral pions were investigated
Jet size dependence of single jet suppression in lead-lead collisions at sqrt(s(NN)) = 2.76 TeV with the ATLAS detector at the LHC
Measurements of inclusive jet suppression in heavy ion collisions at the LHC
provide direct sensitivity to the physics of jet quenching. In a sample of
lead-lead collisions at sqrt(s) = 2.76 TeV corresponding to an integrated
luminosity of approximately 7 inverse microbarns, ATLAS has measured jets with
a calorimeter over the pseudorapidity interval |eta| < 2.1 and over the
transverse momentum range 38 < pT < 210 GeV. Jets were reconstructed using the
anti-kt algorithm with values for the distance parameter that determines the
nominal jet radius of R = 0.2, 0.3, 0.4 and 0.5. The centrality dependence of
the jet yield is characterized by the jet "central-to-peripheral ratio," Rcp.
Jet production is found to be suppressed by approximately a factor of two in
the 10% most central collisions relative to peripheral collisions. Rcp varies
smoothly with centrality as characterized by the number of participating
nucleons. The observed suppression is only weakly dependent on jet radius and
transverse momentum. These results provide the first direct measurement of
inclusive jet suppression in heavy ion collisions and complement previous
measurements of dijet transverse energy imbalance at the LHC.Comment: 15 pages plus author list (30 pages total), 8 figures, 2 tables,
submitted to Physics Letters B. All figures including auxiliary figures are
available at
http://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/HION-2011-02
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