11 research outputs found
Anti-metastatic and differential effects on protein expression of epigallocatechin-3-gallate in HCCLM6 hepatocellular carcinoma cells
Hepatocellular carcinoma (HCC) is the fifth most common cancer worldwide and the third highest cause of cancer-related mortality in humans. Epigallocatechin-3-gallate (EGCG) has been shown to inhibit the metastatic activity of certain cancer cells. The aim of this study was to determine the effects and molecular mechanism(s) of action of EGCG in human HCC cells. A migration and invasion assay for the metastatic behavior of HCCLM6 cells was performed. The anti-metastatic effects of EGCG were investigated by RT-PCR and gelatin zymography. A total cellular protein profile was obtained using 2-dimensional gel electrophoresis (2-DE), followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) analyses of proteins with significant differences in expression following treatment with EGCG. The results revealed that EGCG induced apoptosis and inhibited the metastasis of HCCLM6 cells. The anti-metastatic effects of EGCG were associated with the inhibition of matrix metalloproteinase (MMP)-2 and MMP-9 activity. The expression levels of far upstream element (FUSE) binding protein 1 (FUBP1), heat shock protein beta 1 (HSPB1), heat shock 60 kDa protein 1 (chaperonin) (CH60) and nucleophosmin (NPM) proteins, which are associated with metastasis, were significantly altered in the EGCG-treated HCCLM6 cells. The data from the present study suggest that EGCG has potential as a therapeutic agent for the treatment of HCC
Alpha-Lipoic Acid Functionalized Silver Nanoparticles for Colorimetric Detection of Copper Ion
Not Available
Not AvailableEpigallocatechin-3-gallate (EGCG), a
very potent antioxidant derived from green tea, was
compared with vitamin E in terms of its effects on
antioxidant defense and immune response of rainbow
trout, by means of a feeding trial of eight weeks. Two
of the experimental diets were supplemented with
EGCG at either 20 or 100 mg kg-1 diet (which
contained only 30% of the intended levels) and the
third was provided with 100 mg kg-1 vitamin E but
not EGCG. The control diet was not supplemented
with the test components. Observation of tissue levels
indicated that the high amount of EGCG helped to increase the availability of the lipid-soluble antiox idant vitamin E. The lower levels of lipid hydroper oxide in the liver of fish fed the higher amount of
EGCG suggested that it was an effective antioxidant.
Considering the immune indices, EGCG and vitamin
E at 100 mg (actual amounts 31.9 and 94.1 mg kg-1
diet, respectively) had identical capabilities in
improving phagocytic activity and controlling hydro gen peroxide production by leucocytes. However,
EGCG could possibly be more effective at enhancing
serum lysozyme activity and the alternative comple ment activity. This work revealed the potential of
EGCG as an antioxidant and an immunostimulant for
rainbow trout, at least at the inclusion level of
32 mg kg-1 diet.Not Availabl