Genetic Potential and Usefulness of Native Maize Populations in Developing Novel Germplasm for Current and Upcoming Goals

Traditional agricultural system is referring to the maize production based on indigenous or farmers knowledge and practices that have been developed through many generations. In the area of study, genetic maize diversity was explored by the expression of quantitative traits of the ear and the race classification approach. Evaluation results indicated that the native populations adapted to the transition and highland (above 2000 masl) areas, showed a contrasting yield response when they were evaluated at the intermediate environment; whereas, those populations adapted to the lowland and intermediate altitudes showed a satisfactory yield performance in both environments. The above performance pattern is essential because it may be useful to identify favorable alleles that, in a local population per se or through genetic combination, results in population changes in allele frequencies that could mitigate the effects of climate changes, particularly in maize populations adapted to highland altitudes. Selection procedures applied to a local adapted population can be managed attending different goals, including the conservation of genetic diversity (per se selection), and to develop novel germplasm. The introgression of foreign germplasm into a local population and the application of three selection cycles resulted in a novel variety (JAGUAN) adapted to a regional northeast Mexico environmental conditions

Agronomic performace in acid soils of wheat lines selected for hematoxylin staining pattern

Laboratory screening techniques enable detection of varying degrees of A1 tolerance during seedling development, but their application to crop improvement programs must be verified in the field. The objective of this study was to compare agronomic responses of wheat (Triticum aestivum L. emend. Thell.) lines differing in hematoxylin- staining pattern under naturally acidic and limed field conditions. Experiments were conducted on Bethany-Kirkland (fine, mixed, thermic Pachic-Udertic Paleustolls) or Grant-Pond Creek (fine-silty, mixed thermic Udic-Pachic Argiustolls) silt loam soil complexes. Four sets of three experimental lines were classified as very susceptible (VS), moderately susceptible (MS), intermediate (I), or tolerant based on hematoxylin staining pattern, and evaluated in limed and unlimed plots as separate experiments in 1988 and 1989. The VS group produced the fewest spikes per square meter and the lowest above-ground biomass and grain yield in the unlimed treatments; no such reductions occurred in the limed treatments. Differences in agronomic performance were found between groups of VS and MS lines, with relatively little differentiation among MS, I, and T groups. Comparison of two sister lines from the VS and T groups revealed a 12% increase in spike density, 11% increase in biomass, and a 14% increase in grain yield of the tolerant sib under acidic conditions. The two sibs performed similarly under limed conditions, except the VS line was superior in grain yield. The hematoxylin staining procedure shows promise for identifying wheat genotypes with potentially high sensitivity to acidic soils in the southern Great Plains.Peer reviewedAgronom

Agronomic characterization of pigmented native corn populations (Zea mays L.)

ABSTRACT Objective: We performed the agronomical characterization of 52 pigmented corn populations from the state of Coahuila in order to identify those with outstanding agronomical potential and also to establish the genetic background of a breeding program focused in the improvement of varieties with grain yield potential, adaptation capacity to the regional production conditions and of superior nutritional and bioactive contents. Design/methodology: Through an incomplete block design in an alpha lattice arrangement, we evaluated two experiments in two representative locations of the southeastern agricultural area of Coahuila: a) Yellow populations, mostly from Tuxpeño, Ratón and Tuxpeño Norteño races and b) Anthocyanic populations (blue, red and purple), mainly represented by Cónico Norteño, Elotes Cónicos and Ratón races. Results: The results showed a contrasting environmental effect between locations, with differences in grain yield up to 78 %; a 25 days’ difference in flowering, 53 cm in plant height and up to 8 plants without ear; this performance was due to the agricultural potential of each location. We observed that in both locations the yellow populations outstanding by their superior performance were: from the Tuxpeño race: COAH068, COAH089, COAH177 and COAH215, and Celaya race: COAH075. The outstanding anthocyanin populations were: Ratón race: COAH23 and COAH203, Elotes Cónicos race: COAH246 and COAH019, and Elotes Occidentales race: COAH021. Also we found populations with adaptation to a location. Conclusions: We consider that among the diversity of the pigmented corn from Coahuila there are populations of superior agronomic expression that could be used as the genetic background to improve the production and the nutritional quality of the grain and the food products that can be derived from them.Objective: To characterize agronomically 52 populations of pigmented native corn (Zea mays L.) from Coahuila, Mexico, in order to identify varieties with outstanding agronomic potential and to establish an improvement program with potential for grain yield, with the ability to adapt, and superior nutritional bioactive content. Design/Methodology: Two experiments were evaluated through an incomplete block design in lattice alpha arrangement, in two localities that are representative of the agricultural area of southeastern Coahuila: a) Yellow populations, mostly of Tuxpeño, Ratón and Tuxpeño Norteño; and b) Anthocyanin populations (blue, red and purple), represented primarily by Cónico Norteño, Elotes Cónicos and Ratón. Results: There is an environmental effect that contrasts between localities, with differences in grain yield of up to 78%; 25 days of difference in flowering, 53 cm in plant height, and up to seven plants without cobs. The response was product of the agricultural potential in each locality. The outstanding yellow populations due to their superior yield expression in both localities were the landraces Tuxpeño: COAH068, COAH089, COAH177 and COAH215, and Celaya: COAH075. The outstanding anthocyanin populations were Ratón: COAH23 and COAH203; Elotes Cónicos: COAH246 and COAH019; and Elotes Occidentales: COAH021; in addition, the study found populations adapted to a locality. Conclusion: Among the diversity of the pigmented native corn in Coahuila, there are populations with superior agronomic expression that can be the basis for improving the production, the nutritional quality of the grain, and therefore, of its byproducts

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Phenotypical, Clinical, and Molecular Aspects of Adults and Children With Homozygous Familial Hypercholesterolemia in Iberoamerica

Fil: Alves, Ana Catarina. Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa; Portugal.Fil: Alonso, Rodrigo. Center for Advanced Metabolic Medicine and Nutrition, Santiago; Chile.Fil: Diaz-Diaz, José Luís. Hospital Universitario A Coruña. Department of Internal Medicine; España.Fil: Medeiros, Ana Margarida. Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa; Portugal.Fil: Jannes, Cinthia E. University of São Paulo. Medical School. Hospital São Paulo. Heart Institute (InCor); Brasil.Fil: Merchan, Alonso. Fundación Clinica SHAIO, Cardiología, Bogotá; Colombia.Fil: Vasques-Cardenas, Norma A. Universidad Autónoma de Guadalajara. Facultad de Medicina Zapopan; México.Fil: Cuevas, Ada. Center for Advanced Metabolic Medicine and Nutrition, Santiago; Chile.Fil: Chacra, Ana Paula. University of São Paulo. Medical School. Hospital São Paulo. Heart Institute (InCor); Brasil.Fil: Krieger, Jose E. University of São Paulo. Medical School. Hospital São Paulo. Heart Institute (InCor); Brasil.Fil: Arroyo, Raquel. Fundación Hipercolesterolemia Familiar, Madrid; España.Fil: Arrieta, Francisco. Hospital Ramón y Cajal. Departamento de Endocrinología, Madrid; España.Fil: Schreier, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica, Laboratorio de Lípidos y Aterosclerosis; Argentina.Fil: Corral, Pablo. Universidad FASTA. Facultad de Medicina. Cátedra Farmacología e Investigación, Mar del Plata; Argentina.Fil: Bañares, Virginia. ANLIS Dr.C.G.Malbrán. Centro Nacional de Genética Médica. Departamento de Genética Experimental; Argentina.Fil: Araujo, Maria B. Hospital Garrahan. Servicio de Nutrición; Argentina.Fil: Bustos, Paula. Universidad de Concepción. Facultad de Farmacia; Chile.Fil: Asenjo, Sylvia. Universidad de Concepción. Facultad de Medicina; Chile.Fil: Stoll, Mario. Programa GENYCO, Laboratorio de Genética Molecular. Comisión Honoraria de Salud Cardiovascular, Montevideo; Uruguay.Fil: Dell'Oca, Nicolás. Programa GENYCO, Laboratorio de Genética Molecular. Comisión Honoraria de Salud Cardiovascular, Montevideo; Uruguay.Fil: Reyes, Maria. Fundación Cardiovascular de Colombia. Cardiología; Bogotá.Fil: Ressia, Andrés. Fundación Cardiovascular de Colombia. Cardiología; Bogotá.Fil: Campo, Rafael. Instituto Mexicano del Seguro Social. Centro de Investigación Biomédica del Occidente, Guadalajara; México.Fil: Magaña-Torres, Maria T. Instituto Nacional de Ciencias Médicas y Nutrición. Unidad de Investigación de Enfermedades Metabólicas; México.Fil: Metha, Roopa. Instituto Nacional de Ciencias Médicas y Nutrición. Unidad de Investigación de Enfermedades Metabólicas; México.Fil: Aguilar-Salinas, Carlos A. Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán. Departamento de Endocrinología y Metabolismo. Secretaría de la Defensa Nacional. Unidad de Especialidades Médicas. Servicio de Endocrinología; México.Fil: Ceballos-Macias, José J. Pontificia Universidad Javerina. Facultad de Medicina. Departamento de Medicina Interna, Bogotá; Colombia.Fil: Ruiz Morales, Álvaro J. Pontificia Universidad Javerina. Facultad de Medicina. Departamento de Medicina Interna, Bogotá; Colombia.Fil: Mata, Pedro. Fundación Hipercolesterolemia Familiar, Madrid; España.Fil: Bourbon, Mafalda. Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisboa; Portugal.Fil: Santos, Raul D. University of São Paulo. Medical School. Hospital São Paulo. Heart Institute (InCor); Brasil.OBJECTIVE: Characterize homozygous familial hypercholesterolemia (HoFH) individuals from Iberoamerica. APPROACH AND RESULTS: In a cross-sectional retrospective evaluation 134 individuals with a HoFH phenotype, 71 adults (age 39.3±15.8 years, 38.0% males), and 63 children (age 8.8±4.0 years, 50.8% males) were studied. Genetic characterization was available in 129 (96%). The majority (91%) were true homozygotes (true HoFH, n=79, 43.0% children, 46.8% males) or compound heterozygotes (compound heterozygous familial hypercholesterolemia, n=39, 51.3% children, 46.2% males) with putative pathogenic variants in the LDLR. True HoFH due to LDLR variants had higher total (P=0.015) and LDL (low-density lipoprotein)-cholesterol (P=0.008) compared with compound heterozygous familial hypercholesterolemia. Children with true HoFH (n=34) tended to be diagnosed earlier (P=0.051) and had a greater frequency of xanthomas (P=0.016) than those with compound heterozygous familial hypercholesterolemia (n=20). Previous major cardiovascular events were present in 25 (48%) of 52 children (missing information in 2 cases), and in 43 (67%) of 64 adults with LDLR variants. Children who are true HoFH had higher frequency of major cardiovascular events (P=0.02), coronary heart (P=0.013), and aortic/supra-aortic valve diseases (P=0.022) than compound heterozygous familial hypercholesterolemia. In adults, no differences were observed in major cardiovascular events according to type of LDLR variant. From 118 subjects with LDLR variants, 76 (64%) had 2 likely pathogenic or pathogenic variants. In 89 subjects with 2 LDLR variants, those with at least one null allele were younger (P=0.003) and had a greater frequency of major cardiovascular events (P=0.038) occurring at an earlier age (P=0.001). CONCLUSIONS: There was a high frequency of cardiovascular disease even in children. Phenotype and cardiovascular complications were heterogeneous and associated with the type of molecular defect

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication

Respuestas de lechuga a la conductividad eléctrica con riego superficial y subirrigación

Salinity suppresses plant growth and reduce agricultural productivity due to a water deficit, ion toxicity, causing nutrient skewness. A sub-irrigation system on principle, is based on capillary rise of water, up to the root zone, decreasing nutrient leaching compared with traditional watering systems. In void areas of northern Mexico, there are often high levels of calcium (Ca), magnesium and sulphate in irrigation water, directly affecting the electrical conductivity (EC). This paper proposed to evaluate the effect of EC on the growth of lettuce plants using two types of irrigation systems: surface and sub-irrigation. The diameter of the plants was bigger in plants with surface irrigation system compared to those with sub-irrigation; however, plants sub-irrigated with solution 3.3 dS m-1 showed a higher fresh weight than those with surface irrigation, so this system is a good option to meliorate the effect of salinity. The rate of photosynthesis, transpiration and leaf conductance were not altered by the irrigation system at all, but transpiration and conductance were higher in sub-irrigated plants. A higher EC of the nutrient solution increased the bottom EC of the substrate, and the middle and top layer, and the surface irrigation system was higher compared to that presented by sub-irrigation. High EC of the nutrient solution did not attain the concentration of nitrogen or phosphorus whatsoever, but at elevated EC of the substance, the foliar potassium concentration did increased.La salinidad inhibe el crecimiento de las plantas y reduce la productividad agrícola a causa de un déficit hídrico, toxicidad por los iones que la inducen y al desbalance nutrimental que ocasiona. Un sistema de subirrigación parte del principio del ascenso capilar del agua hasta la zona radical, reduciendo la lixiviación de nutrimentos en comparación con los sistemas tradicionales de riego. En las zonas áridas del norte de México se presentan con frecuencia altos niveles de calcio (Ca), magnesio y sulfato en el agua de riego, lo que impacta directamente en la conductividad eléctrica (CE). El presente estudio se planteó con el objetivo de evaluar el efecto de la CE sobre el crecimiento de plantas de lechuga empleando dos tipos de sistemas de riego: superficial y subirrigación. El diámetro de las plantas fue mayor en plantas con riego superficial en comparación con aquellas con subirrigación; sin embargo, las plantas subirrigadas con solución de 3.3 dS m-1 mostraron un mayor peso fresco que aquellas con riego superficial, por lo que este sistema es una buena opción para mitigar el efecto por salinidad en lechuga. La tasa de fotosíntesis neta, transpiración y conductancia de la hojas no fueron afectadas por el sistema de riego empleado, pero la transpiración y conductancia fueron mayores en plantas subirrigadas. A mayor CE de la solución nutritiva se presentó un aumento en la CE de la parte inferior del sustrato, así como del estrato medio y superior, y con riego superficial esta fue mayor en comparación con subirrigación. La alta CE de la solución nutritiva no afectó la concentración de nitrógeno ni de fosforo, pero al elevarse la CE de la solución se incrementó la concentración foliar de potasio

Agronomic Evaluation and Physiological Quality in Triticale, under Two Production Systems in the Laguna Region

Objective: To evaluate ten triticale genotypes to assess the quality of the seeds under two production systems, with cut and without cut, in the municipalities of Matamoros (L1) and Francisco I. Madero (L2), Coahuila, Mexico. Design/Methodology/Approach: The agronomic variables evaluated were: Grain yield (GY), number of seeds per spike (NSS), spike length (SL), number of spikelets per spike (SS) and plant height (PH). Variables in the laboratory were: normal seedlings (NS), abnormal seedlings (AS), seeds without germinating (SWG), dry seedling weight (DW), plumule length (PL) and radicle length (RL). Statistical analysis was performed with the PROC ANOVA procedure, of the SAS software. Results: A higher grain yield was found for Matamoros (L1) compared to Francisco I. Madero (L2); however, for the variable number of seeds per spike it was higher in the latter (L2). For the physiological quality of seeds in L2, the highest values were in the variables normal seedlings and radicle length. In the comparison of production systems, the best response was for the system without cut in both locations; therefore, good quality triticale seed can be produced with acceptable grain yield. Study Limitations/Implications: It is necessary to describe the performance of triticale in the spring-summer agricultural cycle in the central region of the country.Findings/ Conclusions: The productive performance of triticale genotypes was variable depending on the production system, and there was also a different effect on the physiological quality of the harvested seed