Adenovirus E1A proteins are closely associated with chromatin in productively infected and transformed cells

AbstractThe adenovirus E1A 243R oncoprotein encodes a potent transcription-repression function within the N-terminal 80 amino acids. Our proposed model of E1A repression predicts that E1A interacts with important cellular proteins on chromatin. Consistent with this idea, we report here that E1A proteins from in vivo formaldehyde cross-linked 293 cells are closely associated with chromatin even after several stringent purification steps including double isopycnic CsCl density gradient centrifugation and size exclusion chromatography. Likewise, E1A proteins expressed from virus during productive infection of HeLa cells are closely associated with chromatin starting at early times after infection. No other adenoviral proteins are necessary for E1A 243R protein to associate with chromatin. Analyses of chromatin from HeLa cells infected with adenovirus vectors expressing E1A 243R protein with deletions in different E1A functional domains indicate that sequences within the E1A N-terminal repression domain are needed for the majority of E1A's interactions with chromatin

What is the risk of death or severe harm due to bone cement implantation syndrome among patients undergoing hip hemiarthroplasty for fractured neck of femur? A patient safety surveillance study.

OBJECTIVE: To estimate the risk of death or severe harm due to bone cement implantation syndrome (BCIS) among patients undergoing hip hemiarthroplasty for fractured neck of femur. SETTING: Hospitals providing secondary and tertiary care throughout the National Health Service (NHS) in England and Wales. PARTICIPANTS: Cases reported to the National Reporting and Learning System (NRLS) in which the reporter clearly describes severe acute patient deterioration associated with cement use in hip hemiarthroplasty for fractured neck of femur (assessed independently by two reviewers). OUTCOME MEASURES: Primary-number of reported deaths, cardiac arrests and periarrests per year. Secondary-timing of deterioration and outcome in relation to cement insertion. RESULTS: Between 2005 and 2012, the NRLS received 62 reports that clearly describe death or severe harm associated with the use of cement in hip hemiarthroplasty for fractured neck of femur. There was one such incident for every 2900 hemiarthroplasties for fractured neck of femur during the period. Of the 62 reports, 41 patients died, 14 were resuscitated from cardiac arrest and 7 from periarrest. Most reports (55/62, 89%) describe acute deterioration occurring during or within a few minutes of cement insertion. The vast majority of deaths (33/41, 80%) occurred on the operating table. CONCLUSIONS: These reports provide narrative evidence from England and Wales that cement use in hip hemiarthroplasty for fractured neck of femur is associated with instances of perioperative death or severe harm consistent with BCIS. In 2009, the National Patient Safety Agency publicised this issue and encouraged the use of mitigation measures. Three-quarters of the deaths in this study have occurred since that alert, suggesting incomplete implementation or effectiveness of those mitigation measures. There is a need for stronger evidence that weighs the risks and benefits of cement in hip hemiarthroplasty for fractured neck of femur

3D inkjet printing of electronics using UV conversion

The production of electronic circuits and devices is limited by current manufacturing methods that limit both the form and potentially the performance of these systems. Additive Manufacturing (AM) is a technology that has been shown to provide cross sectoral manufacturing industries with significant geometrical freedom. A research domain known as Multi-Functional Additive Manufacturing (MFAM) in its infancy looks to couple the positive attributes of AM with application in the electronics sector could have a significant impact on the development of new products, however there are significant hurdles to overcome. This paper reports on the single step MFAM of three dimensional (3D) electronic circuitry within a polymeric structure using a combination of conductive and non-conductive materials within a single material jetting based AM system. The basis of this breakthrough is a study of the optical absorption regions of a silver nanoparticle (AgNP) conductive ink which lead to a novel method to rapidly process and sinter silver nanoparticle inks in ambient conditions using simple UV radiation contemporaneously with UV-curing of deposited polymeric structures

UNBOUND

Featured here, are the extraordinary works of our graduating Fanshawe Design class. This accomplishment is truly a celebration of the three years of passion, hard work, and dedication put forth by our students. It is our greatest hope that family, friends and the fashion industry will enjoy the creative endeavors of these emerging designers from the Fashion Design program at Fanshawe College in London, Ontario.https://first.fanshawec.ca/famd_design_fashiondesign_unbound/1001/thumbnail.jp

Research and innovation identified to decarbonise the maritime sector

The maritime sector requires technically, environmentally, socially, and economically informed pathways to decarbonise and eliminate all emissions harmful to the environment and health. This is extremely challenging and complex, and a wide range of technologies and solutions are currently being explored. However, it is important to assess the state-of-the-art and identify further research and innovation required to accelerate decarbonisation. The UK National Clean Maritime Research Hub have identified key priority areas to drive this process, with particular focus on marine fuels, power and propulsion, vessel efficiency, port operations and infrastructure, digitalisation, finance, regulation, and policy

Clinical leadership: a role for students?

Since the publication of the 'To Err is Human' report, patient safety and quality improvement has been an increasingly important agenda in health care. This article explores the role that students can play in leading change in health-care systems