O języku polskim w Burgundii

Zadanie pt. Digitalizacja i udostępnienie w Cyfrowym Repozytorium Uniwersytetu Łódzkiego kolekcji czasopism naukowych wydawanych przez Uniwersytet Łódzki nr 885/P-DUN/2014 zostało dofinansowane ze środków MNiSW w ramach działalności upowszechniającej naukę

Possibilities in the application of solid lipid nanoparticles in combination with 5-fluorouracil to overcome the drugresistance of non-small cell lung cancer cell line A549

Introduction: Multidrug resistance of non-small cell lung cancer cells is associated with a high percentageof therapeutic failures. The aim of this study was to assess the ability of solid lipid nanoparticles as atransporter of the conventionally used cytostatic (5-fluorouracil) to overcome the resistance of A549 cells.Material and methods: MTT assay was used to assess the differences in viability of cells treated with5-fluorouracil alone or in combination with different types of solid lipid nanoparticles. Type of cell deathand distribution of cell cycle phases were evaluated using flow cytometry.Results: The use of nanoparticles as a 5-fluorouracil transporter reduced the viability of A549 cells to agreater extent than the cytostatic alone. This was mainly due to the increase in apoptosis, but also necrosisand cell cycle arrest.Conclusion: Our results indicate the great potential of nanotechnology in the treatment of non-small celllung cancer. By using nanoparticles, it is possible to sensitise tumour cells to cytostatics to which theyare normally resistant. In addition, literature data confirm the safety of solid lipid nanoparticle application

The influence of house dust extract on normal lung cell Mrc5 and non-small lung carcinoma A549

Introduction. Desensitisation is a therapeutic method of allergic disease treatment, but its impact on the cellular level remains to be elucidated. The purpose of this study was to identify the influence of house dust extract on two cell lines types: non-small lung cancer A549 and non-cancerous lung fibroblast Mrc5. Furthermore, we analysed cell viability, type of cell death, and reorganisation of mainly cytoskeletal proteins such as vimentin, F-actin, and b-tubulin. Material and methods. To determine the cell viability, the MTT test was used. The type of cell death was analysed using double staining of annexin V and iodide propidium. The reorganisation of cytoskeletal proteins was evaluated by fluorescent staining and microscopy observation. Results. Our data presented non-statistical differences in a population of live, apoptotic, and necrotic cells. We did not observe significant abnormalities in cytoskeletal reorganisation. Moreover, Mrc5 cell line exhibited a lower sensitivity for house dust extract in comparison to A549 cell line. Conclusions. Our study suggests that desensitisation has no significant influence on survival and cytoskeleton in both cell lines, which correlate with potential use of this method in cancer patients. Obviously, the choice of these kinds of treatment should be used carefully in consultation with a specialist. Additionally, to our knowledge, it was the first presentation of the influence of house dust extract on cells in the context of the main cytoskeletal reorganisations.

Kultura i społeczeństwo na Środkowym Nadodrzu w XIX i XX wieku

303 s. : il., portr.

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins

Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.Peer reviewe

Ethos of nurses in the historical aspect

This works aims at proving the thesis of timeliness of Hippocratic /460- 377 BC/ ethos, Christian ethics and personal model of F. Nightingale /1820-1910/ in the ethos of nurses in the aspect of history. The concept of “ethos” means morality as well as these significant conducts, being the affirmation of specific values distinguishing professional group of nurses. The thought of this thesis focuses on the description of nurses professions ethos in historical aspect, from the times of Hippocrates until 1945 AD. This ethos in historical aspect can also be developed by means of personal standards of moral and nurses authorities and first of all by the principles of Hippocratic ethics, Hippocrates’ ethos and Christian ethics/love of God and man, the charity/ and deontological codes referring to a given specialization of nurses profession. Ethical assumptions of Hippocrates, due to their conformity with natural law, high estimation of life since conception till natural death, respect towards the sick and nurses authorities, the secret of treating, faithfulness to the principle “Primum non nocere”, “Salus aegroti suprema lex esto”, bringing relief in suffering and oath directed to gods, have humanistic, general character and that is why they were adopted by ethos of nurses.Celem pracy jest udowodnienie tezy, zakładającej aktualność etosu hipokratesowgo, etyki chrześcijańskiej i wzorców osobowych w etosie pielęgniarek analizowanym w aspekcie historycznym. Pojęcie „etos” oznacza określoną moralność, jak i te znamienne postawy, będące afirmacją określonych wartości charakteryzujących grupę zawodową pielęgniarek. Idea tej pracy koncentruje się na omawianiu etosu pielęgniarek w aspekcie historycznym od czasów Hipokratesa /460-377 p.n.e./ do 1945 roku. Etos ten może być również kształtowany przez wzorce osobowe autorytetów moralnych i pielęgniarek, ale przede wszystkim przez zasady etyki medycznej Hipokratesa, etos hipokratesowy, etykę chrześcijańską/ miłość Boga i bliźniego, cnota miłosierdzia/ i kodeksy deontologiczne adekwatne do danej specjalizacji zawodów pielęgniarek. Założenia etyczne Hipokratesa ze względu na zgodność z prawem naturalnym, wysoką ocenę wartości życia od poczęcia do śmierci naturalnej, szacunek do chorego i autorytetów pielęgniarek, tajemnicę leczenia, wierność zasadom „Przede wszystkim nie szkodzić”, „Zdrowie chorego najwyższym prawem”, niesienie ulgi w cierpieniu i przysięgę skierowaną do bogów, mają wymiar humanistyczny, powszechny i dlatego zostały one przejmowane przez kształtujący się etos pielęgniarek

Inverted Internal Limiting Membrane Flap Technique: Is It the Best Option for Macular Holes?

Surgical treatment is generally necessary to repair full-thickness macular holes (FTMH). Although vitrectomy with or without internal limiting membrane (ILM) peeling remains the standard surgical technique, the inverted ILM flap procedure has increasingly assumed a role in the primary surgical repair of FTMHs. Some vitreoretinal surgeons reserve this technique to treat large or myopic holes, whereas others use it routinely in all cases. This paper is a comprehensive review of the current scientific evidence on the anatomical and functional outcomes of the inverted ILM flap technique in the repair of macular holes, following the International Vitreomacular Traction Study (IVTS) group classification.info:eu-repo/semantics/publishedVersio