EFFECT OF ROSMARINIC ACID ON SERTOLI CELLS APOPTOSIS AND SERUM ANTIOXIDANT LEVELS IN RATS AFTER EXPOSURE TO ELECTROMAGNETIC FIELDS

Rosmarinic acid belongs to the group of polyphenols; it has antioxidant, anti-inflammatory and antimicrobial activities and help to prevent cell damage caused by free radicals. The objective was to study the effect of Rosmarinic acid on sertolli cells apoptosis and serum antioxidant levels in rats after they were exposed to electromagnetic fields. Male Wistar rats (n=40) were allocated into three groups: control group (n=10) that received 5cc normal saline (0.9% NaCl) daily by gavage method, Rosmarinic acid group that received 5mg/rat (gavage) (n=10), electromagnetic fields (EMF) group that had exposure with 50hz (n=20) which was subdivided to two groups of 10; EMF group and treatment group. Treatment group received 5mg/rat (gavage) Rosmarinic acid daily for 6weeks, respectively. However, the control group just received an equal volume of distilled water daily (gavage). On the 42nd day of research, 5cc blood was collected to measure testosterone hormones, total antioxidant capacity (TAC), levels from whole group’s analysis. Level of malondialdehyde (MDA) levels and sertoli cells apoptosis significantly decreased in the group that received 5mg/rat of Rosmarinic acid (

The global, regional, and national burden of cirrhosis by cause in 195 countries and territories, 1990-2017 : a systematic analysis for the Global Burden of Disease Study 2017

Background Cirrhosis and other chronic liver diseases (collectively referred to as cirrhosis in this paper) are a major cause of morbidity and mortality globally, although the burden and underlying causes differ across locations and demographic groups. We report on results from the Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2017 on the burden of cirrhosis and its trends since 1990, by cause, sex, and age, for 195 countries and territories. Methods We used data from vital registrations, vital registration samples, and verbal autopsies to estimate mortality. We modelled prevalence of total, compensated, and decompensated cirrhosis on the basis of hospital and claims data. Disability-adjusted life-years (DALYs) were calculated as the sum of years of life lost due to premature death and years lived with disability. Estimates are presented as numbers and age-standardised or age-specific rates per 100 000 population, with 95% uncertainty intervals (UIs). All estimates are presented for five causes of cirrhosis: hepatitis B, hepatitis C, alcohol-related liver disease, non-alcoholic steatohepatitis (NASH), and other causes. We compared mortality, prevalence, and DALY estimates with those expected according to the Socio-demographic Index (SDI) as a proxy for the development status of regions and countries. Findings In 2017, cirrhosis caused more than 1.32 million (95% UI 1.27-1.45) deaths (440000 [416 000-518 000; 33.3%] in females and 883 000 [838 000-967 000; 66.7%] in males) globally, compared with less than 899 000 (829 000-948 000) deaths in 1990. Deaths due to cirrhosis constituted 2.4% (2.3-2.6) of total deaths globally in 2017 compared with 1.9% (1.8-2.0) in 1990. Despite an increase in the number of deaths, the age-standardised death rate decreased from 21.0 (19.2-22.3) per 100 000 population in 1990 to 16.5 (15.8-18-1) per 100 000 population in 2017. Sub-Saharan Africa had the highest age-standardised death rate among GBD super-regions for all years of the study period (32.2 [25.8-38.6] deaths per 100 000 population in 2017), and the high-income super-region had the lowest (10.1 [9.8-10-5] deaths per 100 000 population in 2017). The age-standardised death rate decreased or remained constant from 1990 to 2017 in all GBD regions except eastern Europe and central Asia, where the age-standardised death rate increased, primarily due to increases in alcohol-related liver disease prevalence. At the national level, the age-standardised death rate of cirrhosis was lowest in Singapore in 2017 (3.7 [3.3-4.0] per 100 000 in 2017) and highest in Egypt in all years since 1990 (103.3 [64.4-133.4] per 100 000 in 2017). There were 10.6 million (10.3-10.9) prevalent cases of decompensated cirrhosis and 112 million (107-119) prevalent cases of compensated cirrhosis globally in 2017. There was a significant increase in age-standardised prevalence rate of decompensated cirrhosis between 1990 and 2017. Cirrhosis caused by NASH had a steady age-standardised death rate throughout the study period, whereas the other four causes showed declines in age-standardised death rate. The age-standardised prevalence of compensated and decompensated cirrhosis due to NASH increased more than for any other cause of cirrhosis (by 33.2% for compensated cirrhosis and 54.8% for decompensated cirrhosis) over the study period. From 1990 to 2017, the number of prevalent cases snore than doubled for compensated cirrhosis due to NASH and more than tripled for decompensated cirrhosis due to NASH. In 2017, age-standardised death and DALY rates were lower among countries and territories with higher SDI. Interpretation Cirrhosis imposes a substantial health burden on many countries and this burden has increased at the global level since 1990, partly due to population growth and ageing. Although the age-standardised death and DALY rates of cirrhosis decreased from 1990 to 2017, numbers of deaths and DALYs and the proportion of all global deaths due to cirrhosis increased. Despite the availability of effective interventions for the prevention and treatment of hepatitis B and C, they were still the main causes of cirrhosis burden worldwide, particularly in low-income countries. The impact of hepatitis B and C is expected to be attenuated and overtaken by that of NASH in the near future. Cost-effective interventions are required to continue the prevention and treatment of viral hepatitis, and to achieve early diagnosis and prevention of cirrhosis due to alcohol-related liver disease and NASH. Copyright (C) 2020 The Author(s). Published by Elsevier Ltd.Peer reviewe

Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-Adjusted life-years for 29 cancer groups, 1990 to 2017 : A systematic analysis for the global burden of disease study

Importance: Cancer and other noncommunicable diseases (NCDs) are now widely recognized as a threat to global development. The latest United Nations high-level meeting on NCDs reaffirmed this observation and also highlighted the slow progress in meeting the 2011 Political Declaration on the Prevention and Control of Noncommunicable Diseases and the third Sustainable Development Goal. Lack of situational analyses, priority setting, and budgeting have been identified as major obstacles in achieving these goals. All of these have in common that they require information on the local cancer epidemiology. The Global Burden of Disease (GBD) study is uniquely poised to provide these crucial data. Objective: To describe cancer burden for 29 cancer groups in 195 countries from 1990 through 2017 to provide data needed for cancer control planning. Evidence Review: We used the GBD study estimation methods to describe cancer incidence, mortality, years lived with disability, years of life lost, and disability-Adjusted life-years (DALYs). Results are presented at the national level as well as by Socio-demographic Index (SDI), a composite indicator of income, educational attainment, and total fertility rate. We also analyzed the influence of the epidemiological vs the demographic transition on cancer incidence. Findings: In 2017, there were 24.5 million incident cancer cases worldwide (16.8 million without nonmelanoma skin cancer [NMSC]) and 9.6 million cancer deaths. The majority of cancer DALYs came from years of life lost (97%), and only 3% came from years lived with disability. The odds of developing cancer were the lowest in the low SDI quintile (1 in 7) and the highest in the high SDI quintile (1 in 2) for both sexes. In 2017, the most common incident cancers in men were NMSC (4.3 million incident cases); tracheal, bronchus, and lung (TBL) cancer (1.5 million incident cases); and prostate cancer (1.3 million incident cases). The most common causes of cancer deaths and DALYs for men were TBL cancer (1.3 million deaths and 28.4 million DALYs), liver cancer (572000 deaths and 15.2 million DALYs), and stomach cancer (542000 deaths and 12.2 million DALYs). For women in 2017, the most common incident cancers were NMSC (3.3 million incident cases), breast cancer (1.9 million incident cases), and colorectal cancer (819000 incident cases). The leading causes of cancer deaths and DALYs for women were breast cancer (601000 deaths and 17.4 million DALYs), TBL cancer (596000 deaths and 12.6 million DALYs), and colorectal cancer (414000 deaths and 8.3 million DALYs). Conclusions and Relevance: The national epidemiological profiles of cancer burden in the GBD study show large heterogeneities, which are a reflection of different exposures to risk factors, economic settings, lifestyles, and access to care and screening. The GBD study can be used by policy makers and other stakeholders to develop and improve national and local cancer control in order to achieve the global targets and improve equity in cancer care. © 2019 American Medical Association. All rights reserved.Peer reviewe

Synthesis and Optical Properties of Gamma-Alumina Nanoparticles and its Antibacterial Properties

Background & Objective: By increasing resistance due to overuse of antibiotics, it seems necessary to find alternative materials that have both antibacterial properties close to antibiotics and have minimal side effects for humans. Abundant research has been done on the use of nanoparticles with antibacterial properties. The aim of this study is to investigate the antibacterial properties of gamma alumina quantum dots. Materials & Methods: Gamma alumina quantum dots (Al2O3-g) were prepared by sol-gel method. Structural, physical and optical properties and of this nanoparticles were appraised by X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), photoluminescence spectroscopy (PL), visible-ultraviolet spectroscopy (UV-Vis) and Fourier transform infrared (FTIR) spectroscopy. Biological activity of gamma alumina nanoparticles against 11 types of bacteria was measured by MIC and MBC methods. Results: Results show the size of the synthesized nanoparticles is less than ten nanometers. The power of minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) by gamma alumina quantum dots is related to its particle size, which interpenetrates and destroys the bacterial wall more easily. Gamma alumina quantum dots show good activity against bacterial growth. Conclusion: The antibacterial activity of alumina quantum nanoparticles showed desirable effects for a variety of gram-positive and gram-negative bacteria, fungi and yeasts at the lowest inhibitory concentrations. These nanoparticles can be a great alternative to antibiotics and making these nanoparticles is economical, cost-effective and is less time-consuming

Carbon Quantum Dots Extracted from Natural Lemon Juice: Efficient Material for Fluorescence and Antibacterial Applications

Background & Objective: In recent years, the proliferation of microbial organisms has increased alarmingly, and the overuse of various antibiotics against microorganisms has increased drug resistance. On the other hand, the need to reduce health costs, the production of antimicrobials with low costs, and the basic needs of today's human society have become. This led to a large-scale study of new drugs against microorganisms and the use of nanoparticles as antibacterial agents were considered. This study aimed to use biocompatible carbon quantum dots(CQDs) nanoparticles instead of antibiotics resistant to gram-positive and gram-negative microorganisms. Materials & Methods: Fluorescent carbon quantum dots were extracted from natural lemon juice using the hydrothermal approach. Analyzes of X-ray diffraction (XRD), Fourier transform infrared(FT-IR), ultraviolet-visible (UV-Vis), photoluminescence(PL), transmission electron microscope(TEM), and energy-dispersive spectrometer(EDS). CQDs were investigated on ten types of microorganisms by the microwell dilution method. In this study, the minimal inhibition concentrations) MIC) and the minimum bactericidal concentration(MBC) was determined. Results: Fluorescent CQDs less than 5 nm in size were fabricated and confirmed by structural and microscopic analysis. This test showed that four microorganisms B. subtilis, E. coli, P. aeruginosa, S. pyogenes and C. albicans were resistant to the antibiotic nystatin and showed the highest sensitivity to CQDs, the lowest MIC and MBC for CQDs are 250 µg/ml and 1000 µg/ml. Conclusions: In general, the results obtained from this study can claim that CQDs have antibacterial properties and can be introduced after further studies as candidates are used to treat or prevent a variety of infections caused by microorganisms

Green Synthesis of Au-doped SnO2 Nanoparticles Using Teucrium Polium Plant Extract for the Evaluation of Their Physicochemical and Antibacterial Properties

Background & Objective: The biological production of nanoparticles (NPs) is a technique that has garnered a lot of interest because it is inexpensive, straightforward, and friendly to the natural environment. This study explores the green synthesis of Au-doped SnO2 NPs using Teucrium polium plant extract and evaluates their antimicrobial properties in comparison with two commonly used antibiotics. Materials & Methods: Initially, an extract of Teucrium polium was made using water, and then it was combined with solutions of tin (II) chloride dehydrate [SnCl2.2H2O] and gold (III) chloride trihydrate [HAuCl4.3H2O]. The crystal structure was studied using X-ray diffraction (XRD). The form, structure, and grain size of the NPs were analyzed using scanning electron microscope (SEM) and transmission electron microscope (TEM). The synthesized NPs were tested for their antibacterial properties. Results: The XRD and EDS analyses revealed that the SnO2:Au phase formed an average crystallite size of 22 nm and an Au dopant content of around 2%. The SEM and TEM investigations demonstrated that the NPs were formed in a regular and nanoscale manner, with diameters ranging from 25 to 30 nm. MIC and MBC of SnO2: Au NPs against the studied bacteria(S.aureus ATCC 43300, P.aeruginosa PAO1) were reported 9.6±0.13- 9.6±0.07 μg/mL and 25.9±11.5- 32.5±11.2 μg/mL Conclusion: Green-synthesized NPs exhibit significant antimicrobial activity against both Gram-positive and Gram-negative bacteria, even at low concentrations. The NPs effectively eliminate bacteria and prevent biofilm formation

Impact of Visual and Cognitive Distractions and Time Pressure on Pedestrian Crossing Behaviour: A Simulator Study

Distractions have been recognised as one important factor associated with pedestrian injuries, as the increasing use of cell phones and personal devices. However, the situation is less clear regarding the differences in the effects of visual-manual and auditory-cognitive distractions. Here, we investigated distracted pedestrians in a one-lane road with continuous traffic using an immersive CAVE-based simulator. Sixty participants were recruited to complete a crossing task and perform one of two distractions, a visual-manual task and an auditory-cognitive task. Moreover, normal and time pressure crossing conditions were included as a baseline and comparison. For the first time, this study directly compared the impacts of visual-manual, auditory-cognitive distractions, and time pressure on pedestrian crossing behaviour and safety in a controlled environment. The results indicated that although pedestrian safety was compromised under both types of distraction, the effects of the applied distractions were different. When engaged in the visual-manual distraction, participants crossed the road slowly, but there was no significant difference in gap acceptance or initiation time compared to baseline. In contrast, participants walked slowly, crossed earlier, and accepted smaller gaps when performing the auditory-cognitive distraction. This has interesting parallels to existing findings on how these two types of distractions affect driver performance. Moreover, the effects of the visual-manual distraction were found to be dynamic, as these effects were affected by the gap size. Finally, compared to baseline, time pressure resulted in participants accepting smaller time gaps with shorter initiation times and crossing durations, leading to an increase in unsafe decisions and a decrease in near-collisions. These results provide new evidence that two types of distraction and time pressure impair pedestrian safety, but in different ways. Our findings may provide insights for further studies involving pedestrians with different distraction components