72 research outputs found
Metabolomics-driven quantitative analysis of ammonia assimilation in E. coli
Despite extensive study of individual enzymes and their organization into pathways, the means by which enzyme networks control metabolite concentrations and fluxes in cells remains incompletely understood. Here, we examine the integrated regulation of central nitrogen metabolism in Escherichia coli through metabolomics and ordinary-differential-equation-based modeling. Metabolome changes triggered by modulating extracellular ammonium centered around two key intermediates in nitrogen assimilation, α-ketoglutarate and glutamine. Many other compounds retained concentration homeostasis, indicating isolation of concentration changes within a subset of the metabolome closely linked to the nutrient perturbation. In contrast to the view that saturated enzymes are insensitive to substrate concentration, competition for the active sites of saturated enzymes was found to be a key determinant of enzyme fluxes. Combined with covalent modification reactions controlling glutamine synthetase activity, such active-site competition was sufficient to explain and predict the complex dynamic response patterns of central nitrogen metabolites
Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae
CONSTRUCTION OF SECRETION VECTOR AND SECRETION OF hIFN-β11All of this work was carried out under national project supported by the Ministry of International Traae and Industry, Japan
Hybrid anthracycline antibiotics: production of new anthracyclines by cloned genes from Streptomyces purpurascens in Streptomyces galilaeus
Subinhibitory cerulenin inhibits staphylococcal exoprotein production by blocking transcription rather than by blocking secretion
Tight linkage of genes that encode the two glutamate synthase subunits of Escherichia coli K-12
Modulation of the Purine Pathway for Riboflavin Production in Flavinogenic Recombinant Strain of the Yeast Candida famata
SECRETION ACTIVITY OF THE BACILLUS SUBTILIS α-AMYLASE SIGNAL PEPTIDES WITH DIFFERENT LENGTHS IN BACILLUS SUBTILIS AND ESCHERICHIA COLI CELLS11This work was supported by a Grant-In-Aid for Special Distinguished Research from the Ministry of Education, Science and Culture of Japan
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