537 research outputs found

    Studies on membrane protein turnover in growing yeast cells

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    Part of colibacillosis in young calf scours and antimicrobial resistance in France (2011 and 2013)

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    Les gastroentérites néonatales sont une cause majeure de pertes économiques en élevage bovin. Nous avons fait un état des lieux de cette pathologie dans l’Allier entre 2011 et 2013 chez des veaux de moins de huit jours. Escherichia coli , les rotavirus, coronavirus et Cryptosporidium sont recherchés dans les selles. La prévalence des agents pathogènes est supérieure chez les veaux malades, mais d’autres facteurs de risque semblent intervenir. Les niveaux d’antibiorésistance observés sont inquiétants et sont parfois corrélés à l’utilisation des molécules antimicrobiennes. Des mesures préventives sont indispensables pour réduire, face à ces maladies, le recours aux antibiotiques.Diarrhea in newborn calves is a major cause of economic losses in farms. The aim of this study was to update our knowledge about the prevalence of the major pathogens in the French county of Allier between 2011 and 2013. Feces of 125 diarrheic and 61 healthy less than 8-day-old calves have been sampled. Major pathogens ( Escherichia coli , rotavirus, coronavirus and Cryptosporidium ) were looked for. Though the various pathogens were isolated more frequently in diarrheic calves, other factors are also prominent. The antimicrobial resistance is worrying and the use of some of antibiotics is associated with increased resistance in E. coli isolated from unhealthy calves. Prevention is a key factor to face newborn calves’ diarrhea and to reduce the use of antibiotics

    Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.

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    The highly conserved eukaryotic DRG factors are required for efficient translation in a manner redundant with the putative RNA helicase Slh1

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    Eukaryotic and archaeal DRG factors are highly conserved proteins with characteristic GTPase motifs. This suggests their implication in a central biological process, which has so far escaped detection. We show here that the two Saccharomyces cerevisiae DRGs form distinct complexes, RBG1 and RBG2, and that the former co-fractionate with translating ribosomes. A genetic screen for triple synthetic interaction demonstrates that yeast DRGs have redundant function with Slh1, a putative RNA helicase also associating with translating ribosomes. Translation and cell growth are severely impaired in a triple mutant lacking both yeast DRGs and Slh1, but not in double mutants. This new genetic assay allowed us to characterize the roles of conserved motifs present in these proteins for efficient translation and/or association with ribosomes. Altogether, our results demonstrate for the first time a direct role of the highly conserved DRG factors in translation and indicate that this function is redundantly shared by three factors. Furthermore, our data suggest that important cellular processes are highly buffered against external perturbation and, consequently, that redundantly acting factors may escape detection in current high-throughput binary genetic interaction screens

    Fourier transforming a trapped Bose-Einstein condensate by waiting a quarter of the trap period: simulation and applications

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    We investigate the property of isotropic harmonic traps to Fourier transform a weakly interacting Bose–Einstein condensate (BEC) every quarter of a trap period. We solve the Gross–Pitaevskii equation numerically to investigate the time evolution of interacting BECs in the context of the Fourier transform, and we suggest potential applications

    Interactive Visualization of the Largest Radioastronomy Cubes

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    3D visualization is an important data analysis and knowledge discovery tool, however, interactive visualization of large 3D astronomical datasets poses a challenge for many existing data visualization packages. We present a solution to interactively visualize larger-than-memory 3D astronomical data cubes by utilizing a heterogeneous cluster of CPUs and GPUs. The system partitions the data volume into smaller sub-volumes that are distributed over the rendering workstations. A GPU-based ray casting volume rendering is performed to generate images for each sub-volume, which are composited to generate the whole volume output, and returned to the user. Datasets including the HI Parkes All Sky Survey (HIPASS - 12 GB) southern sky and the Galactic All Sky Survey (GASS - 26 GB) data cubes were used to demonstrate our framework's performance. The framework can render the GASS data cube with a maximum render time < 0.3 second with 1024 x 1024 pixels output resolution using 3 rendering workstations and 8 GPUs. Our framework will scale to visualize larger datasets, even of Terabyte order, if proper hardware infrastructure is available.Comment: 15 pages, 12 figures, Accepted New Astronomy July 201

    Spatially and polarization resolved plasmon mediated transmission through continuous metal films

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    The experimental demonstration and characterization is made of the plasmon-mediated resonant transmission through an embedded undulated continuous thin metal film under normal incidence. 1D undulations are shown to enable a spatially resolved polarisation filtering whereas 2D undulations lead to spatially resolved, polarization independent transmission. Whereas the needed submicron microstructure lends itself in principle to CD-like low-cost mass replication by means of injection moulding and embossing, the present paper demonstrates the expected transmission effects on experimental models based on metal-coated photoresist gratings. The spectral and angular dependence in the neighbourhood of resonance are investigated and the question of the excess losses exhibited by surface plasmons is discusse

    Interchanging Interactive 3-d Graphics for Astronomy

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    We demonstrate how interactive, three-dimensional (3-d) scientific visualizations can be efficiently interchanged between a variety of mediums. Through the use of an appropriate interchange format, and a unified interaction interface, we minimize the effort to produce visualizations appropriate for undertaking knowledge discovery at the astronomer's desktop, as part of conference presentations, in digital publications or as Web content. We use examples from cosmological visualization to address some of the issues of interchange, and to describe our approach to adapting S2PLOT desktop visualizations to the Web. Supporting demonstrations are available at http://astronomy.swin.edu.au/s2plot/interchange/Comment: 10 pages, 7 figures, submitted to Publications of the Astronomical Society of Australia. v2. Revised title, revised figure 1, fixed typos, minor additions to future work sectio
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