7 research outputs found
Granuloma hialinizante mĂșltiple pulmonar
- Author
- Publication venue
- 'Elsevier BV'
- Publication date
- Field of study
Weaning from mechanical ventilation in intensive care units across 50 countries (WEAN SAFE): a multicentre, prospective, observational cohort study
- Author
- Abe T.
- Aboshady Abdelrhman
- Abrough Fekri
- Acampo-de Jong Melanie J.
- Acharya S.
- Acharya Subhash P.
- Adderley Jane
- Adiguzel N.
- Agrawal V.K.
- Aguilar G.
- Aguirre Gaston
- Aguirre-Bermeo HernĂĄn
- Ahlström B.
- Akbas TĂŒrkay
- Akker M.
- Al Sadeh G.
- Alamri S.
- Algaba Ăngela
- Ali M.
- Aliberti A.
- Allegue J.M.
- Alvarez Diana
- Amador J.
- Amin P.
- Andersen F.H.
- Ansari S.
- Apichatbutr Yutthana
- Apostolopoulou Olympia
- Arabi Y.
- Aragao Irene CĂ©sar
- Arellano D.
- Arica Mestanza
- Arikan Huseyin
- Arinaga K.
- Arnal J.-M.
- Asano Kengo
- AsĂn-Corrochano M.
- Avalos Cabrera J.M.
- Avila Fuentes S.
- Aydemir Semih
- Aygencel Gulbin
- Azevedo L.
- Bacakoglu Feza
- Badie Julio C.
- Baedorf Kassis E.
- Bai Gabriela
- Balaraj Govindan
- Ballico B.
- Banner-Goodspeed Valerie M.
- Banwarie Preveen
- Barbieri R.
- Baronia Arvind Kumar
- Barrett Jonathan A.
- Barrot L.
- Barrueco-Francioni Jesus Emilio
- Barry Jeffrey
- Bauer Philippe R.
- Bawangade H.
- Beavis Sarah
- Beck E.
- Beduneau G.
- Beehre N.
- Beitler J.
- Belenguer Muncharaz Alberto
- Bellani Giacomo
- Belliato M.
- Bellissima A.
- Beltramelli Rodrigo
- Ben Souissi Asma
- Benitez-Cano A.
- Benlamin Mohamed
- Benslama Abdellatif
- Bento LuĂs Ă.
- Benvenuti Daniela
- Berkius J.
- Bernabe Laura
- Bersten Andrew D.
- Berta G.
- Bertini P.
- Bertram-Ralph Elliott
- Besbes M.
- Bettini L.R.
- Beuret P.
- Bewley Jeremy S.
- Bezzi M.
- Bhakhtiani L.
- Bhandary R.
- Bhowmick Kaushik
- Bihari S.
- Bissett B.
- Blythe D.
- Bocher Simon
- Boedjawan N.
- Bojanowski C.M.
- Boni E.
- Boraso Sabrina
- Borelli M.
- Borello S.
- Borislavova M.
- Bosma Karen J.
- Bottiroli M. A.
- Boyd Owen F.
- Bozbay S.
- Briva A.
- Brochard L.
- Bruel C.
- Bruni Andrea
- Buehner Ulrike
- Bugedo G.
- Bulpa P.
- Burt Karen
- Buscot Matthieu
- Buttera S.
- Cabrera Jorge
- Caccese Roberta
- Caironi P.
- Camporota Luigi
- Canchos Gutierrez Ivan
- Canedo N.
- Cani A.
- Cappellini I.
- Carazo Jesus
- Cardonnet Luis Pablo
- Carpio D.
- Carriedo Demetrio
- Carrillo RamĂłn
- Carvalho J.
- Caser Eliana Bernardete
- Castelli A.
- Castillo Quintero M.
- Castro H.
- Catorze Nuno T.
- Cengiz Melike
- Cereijo E.
- Cerny V.
- Ceunen H.
- Chaintoutis Christos
- Chang Youjin
- Chaparro G.
- Chapman Carmel
- Chau Simon K.
- Chavez C.E.
- Chelazzi Cosimo
- Chelly J.
- Chemouni F.
- Chen Kai
- Chena Ariel
- Chiarandini P.
- Chilton Phil
- Chiumello D.
- Cho Y.-J.
- Chou-Lie Y.
- Chudeau N.
- Cinel İsmail Hakkı
- Cinnella G.
- Clark Michele
- Clark Thomas
- Clarkson Kevin P.
- Clementi S.
- Coaguila Luis
- Codecido Alexis Jaspe
- Collins A.
- Colombo R.
- Conde Juan
- Consales G.
- Cook T.
- Coppadoro A.
- Cornejo Rodrigo A.
- Cortegiani Andrea
- Coxo Cristina M.
- Cracchiolo Andrea Neville
- Crespo Ramirez M.
- Crova P.
- Cruz José David
- Cubattoli L.
- Curto F.
- Czempik Piotr Filip
- d'Andrea R.
- da Silva Ramos F.
- Dangers L.
- Danguy Des DĂ©serts Marc
- Danin P.-E.
- Dantas F.
- Daubin CĂ©dric
- Dawei Wu
- de Haro C.
- de Jesus Montelongo Felipe
- de Mendoza Diego
- de Pablo RaĂșl
- de Pascale Gennaro D.
- de Rosa Silvia
- DecavĂšle Maxens
- Declercq P.-L.
- Deicas Alberto
- del Carmen Campos Moreno M.
- Dellamonica J.
- Delmas B.
- Demirkiran Hilmi
- Demirkiran Oktay
- Dendane T.
- Di Mussi Rosa
- Diakaki C.
- Diaz A.
- Diaz W.
- Dikmen Yalim
- Dimoula Aikaterini
- Doble P.
- Doha Nagwa M.
- Domingos G.
- Dres M.
- Dries D.
- Duggal Abhijit
- Duke Graeme J.
- Dunts Pavel V.
- Dybwik Knut
- Dykyy M.
- Eckert Philippe
- Efe Serdar
- Elatrous S.
- Elay GĂŒlseren
- Elmaryul Abubaker S.
- Elsaadany M.
- Elsayed Hany
- Elsayed Samar
- Emery M.
- Ena S.
- Eng Kevin
- Englert Joshua A.
- Erdogan E.
- Ergin Ozcan P.
- Eroglu Ege
- Escobar M.
- Esen Tekeli Arzu
- Esen F.
- Esquivel Gallegos H.
- Esquivel Alejandro
- Estenssoro Elisa
- Ezzouine H.
- Facchini Alberto
- Faheem Mohammad
- Fanelli V.
- Farina Maria Fernanda
- Fartoukh Muriel Sarah
- Fehrle Lutz
- Feng Feng
- Feng Yufeng
- Fernandez B.
- Fernandez Irene
- Fernandez-Rodriguez M.L.
- Ferrando Carlos
- Ferreira da Silva Maria JoĂŁo
- Ferreruela Mireia
- Ferrier Janet
- Flamm Zamorano Matias JesĂșs
- Flood Laura
- Floris L.
- Fluckiger M.
- Forteza Catalina
- Fortunato A.
- Frans E.
- Frattari A.
- Fredes SebastiĂĄn
- Frenzel Tim
- Fumagalli R.
- Furche M.A.
- Fusari Maurizio
- Fysh E.
- Galeas-Lopez J.L.
- Galerneau L.-M.
- Garcia Olivares P.
- Garcia AnalĂa
- Garcia E.
- Garcia M.F.
- Garlicki JarosĆaw B.
- Garnero Aude
- Garofalo E.
- Gautam Prabha
- Gazenkampf A.
- Gelinotte S.
- Gelormini Domenico
- Ghrenassia Ătienne
- Giacomucci Angelo
- Giannoni Roberto
- Gigante A.
- Glober Nancy King
- Gnesin Paolo
- Goligher E.
- Goligher E.C.
- Gollo Y.
- Gomaa Dina
- Gomero Paredes Rosita
- Gomes R.
- Gomez A.
- Gomez Oscar
- Gomez RaĂșl Alejandro
- Gondim Louise Aline RomĂŁo
- Gonzalez I.
- Gonzalez Manuel E.
- Gonzalez-Castro A.
- Gordillo Romero Orlando
- Gordo Federico
- Gouin Philippe
- Graf Santos J.
- Grainne R.
- Grando Matilde
- Granov Grabovica S.
- Grasselli Giacomo
- Grasso R.
- Grasso S.
- Grimmer Lisa
- Grissom Colin K.
- Gritsan Alexey I.
- Gu Qing
- Guan X.-D.
- Guarracino F.
- Guasch N.
- Guatteri Luca
- Gueret Renaud M.
- Guerot Emmanuel
- Guitard P.-G.
- Gumus Ayça
- Gurjar M.
- Gutierrez Patricia
- Guérin Claude
- GĂŒl Fethi
- Hachimi Abdelhamid
- Hadzibegovic A.
- Hagan Samantha J.
- Hammel Clare
- Han Song J.
- Hanlon Gabrielle C.
- Hashemian Seyed Mohammad Reza
- Heines Serge J.H.Jan Hubert
- Henriksson J.
- Herbrecht Jean Ătienne
- Heredia Orbegoso G.O.
- Hermans Greet M.
- Hermon A.
- Hernandez C.
- Hernandez R.
- Herrera Luis
- Herrera-Gutierrez M.
- Heunks Leo M.A.
- Heunks Leo M.A.
- Hidalgo J.
- Hill Dianne
- Holmquist D.
- Homez M.
- Hongtao Xia
- Hormis Anil P.
- Horner D.
- Hornos Micaela Carmen
- Hou M.
- House S.
- Housni B.
- Hugill Keith
- Humbert L.
- Humphreys Sally K.
- Hunter Stephanie
- Hwa Young Lee
- Iezzi N.
- Ilutovich Santiago
- Inal V.
- Innes R.
- Ioannides Panagiotis
- Iotti Giorgio Antonio
- Ippolito Mariachiara
- Irie Hiromasa
- Iriyama H.
- Itagaki T.
- Izura Javier J.
- Izza Santiago
- Jabeen R.
- Jamaati Hamidreza
- Jamadarkhana Sunil
- Jamoussi Amira
- Jankowski Milosz
- Jaramillo L.A.
- Jeon K.
- Jeong Lee Seok
- Jeswani Deepak
- Jha S.
- Jiang Liangyan
- Jing C.
- Jochmans S.
- Johnstad B.A.
- Jongmin Jongmin
- Joret A.
- Jovanovic B.
- Junhasavasdikul D.
- Jurado Maria Teresa
- Kam E.
- Kamohara H.
- Kane C.
- Kara Iskender
- Karakurt Sait
- Karnjanarachata C.
- Kataoka Jun
- Katayama Shinshu
- Kaushik S.
- Kelebek Girgin Nermin
- Kerr Kathryn
- Kerslake I.
- Khairnar Prakash
- Khalid Abidi
- Khan A.
- Khanna A.K.
- Khorasanee Reza
- Kienhorst Dieneke
- Kirakli C.
- Knafelj Rihard
- Kol Mark R.
- Kongpolprom N.
- Kopitko Csaba
- Korkmaz Ekren P.
- Kubisz-Pudelko A.
- Kulcsar Z.
- Kumasawa Junji
- Kurahashi Kiyoyasu
- Kuriyama Akira
- Kutchak F.
- Laake J.H.
- Labarca Eduardo F.
- Labat F.
- Laborda C.
- Laca Barrera M.A.
- Laffey J.G.
- Lagache Laurie
- Landaverde Lopez Antonio
- Lanspa M.
- Lascari Valeria
- Le Meur M.
- Lee J.
- Lee Jinwoo
- Lee S.H.
- Lee W.-Y.
- Lee Y.J.
- Legernaes T.
- Leiner Tamaas
- Lemiale Virginie
- Leonor T.
- Lepper Philipp Moritz
- Li D.
- Li H.
- Li O.
- Lima Ana Raquel
- Lind D.
- Litton E.
- Liu Jialin
- Liu Ling
- Liu N.
- Llitjos J.-F.
- Llorente Beatriz
- Lopez Nava C.
- Lopez C.E.
- Lopez R.
- Lovazzano Pablo
- Lu M.
- Lucchese F.
- Lugano M.
- Lugo Goytia Gustavo
- Luo H.
- Lynch C.
- Macheda Sebastiano
- Madotto F.
- Madrigal Robles V.H.
- Maggiore S.M.
- Magret Iglesias M.
- Malaga P.
- Mallapura Maheswarappa Harish
- Malpartida G.
- Malyarchikov A.
- Mancebo J.
- Mansson Helena Holm
- Manzano Anaid
- Marey I.
- Marin MarĂa
- Marin N.
- Markman E.
- Martin Dal Gesso Cristina
- Martin Alex
- Martin F.
- Martin-Loeches I.
- Martinez F.
- MartĂnez-Fidalgo Conchita
- Mas A.
- Masaaki S.
- Maseda Emilio
- Massa Eleni V.
- Matamis Dimitrios
- Mattsson Anna
- Maugeri Jessica Giuseppina
- Mccredie Victoria A.
- Mccullough J.
- Mcguinness S.
- Mckown A.
- Medve LĂĄszlĂł
- Mei C.
- Mellado Artigas R.
- Mendes V.
- Mervat Mohamed Khalaf Ebraheim
- Michaux I.
- Mikhaeil M.
- Milagros O.
- Milet Igor
- Millan M.T.
- Minwei Z.
- Mirabella Lucia
- Mishra Sanghamitra
- Mistraletti G.
- Mochizuki Katsunori
- Moerer Onnen
- Moghal Arif
- Mojoli F.
- Molin Alexandre
- Molnar Z.
- Montiel R.
- Montini L.
- Monza G.
- Mora Aznar MarĂa
- Morakul Sunthiti
- Morales M.
- Moreno Torres Daniel
- Morocho Tutillo Diego Rolando
- Motherway C.
- Mouhssine Doumiri
- Mouloudi Eleni K.
- Munoz de Cabo Carlos
- Mustafa Mohamed
- Muthuchellappan R.
- Muthukrishnan M.
- Muttini Stefano
- Muñoz T.
- Nagata Isao
- Nahar Dick
- Nakanishi Misuzu
- Nakayama I.
- Namendys-Silva Silvio A.
- Nanchal R.
- Nandakumar S.
- Nasi Alessandra
- Nasir Kamal
- Navalesi P.
- Naz Aslam T.
- Nga Phan Thuy
- Nichol A.
- Niiyama S.
- Nikolakopoulou S.
- Nikolic Elena
- Nitta Kenichi
- Noc M.
- Nonas Stephanie A.
- Nseir Saad
- Nur Soyturk AyĆe
- Obata Y.
- Oeckler R.
- Oguchi Moe
- Ohshimo Shinichiro
- Oikonomou M.
- Ojados Agueda
- Oliveira Filho W.
- Oliveira M.T.
- Oliveri C.
- Olmos Aitor
- Omura K.
- Orlandi M.C.
- Orsenigo F.
- Ortiz-Ruiz de Gordoa Laura
- Ota Kei
- Ovalle Olmos R.
- Oziemski P.
- Ozkan Kuscu O.
- Ozyilmaz E.
- Pachas Alvarado F.
- Pagella Gonzalo
- Palaniswamy Vijayanand
- Palazon Sanchez E.L.
- Palmese Salvatore
- Pan Guojun
- Pan W.
- Panka Bernardo Amisa
- Papali A.
- Papanikolaou Metaxia N.
- Papavasilopoulou Theonymfi G.
- Parekh A.
- Parke R.
- Parrilla D.
- Parrilla Francisco José
- Pasha T.
- Pasin Laura
- Patel Grisma
- Patel M.
- Pati Basanta Kumar
- Patil J.
- Pattnaik S.
- PatĂŁo Luis
- Paul Daniel
- Pavesi Maurizio
- Pavlotsky V.A.
- Paz E.
- Paz Graciela
- Pecci E.
- Pellegrini C.
- Perbet S.
- Perchiazzi G.
- Pereira T.
- Pereira V.
- Perez Calvo CĂ©sar
- Perez Cheng M.
- Perez Maita Ronald
- Perez Manuel P.
- Perez-Teran P.
- Perez-Torres D.
- Perkins Gavin D.
- Persona P.
- Pesenti A.
- Petnak T.
- Petrova Marina V.
- Peña Padilla Andrea Gabriela
- Peñuelas O.
- Peñuelas Ă.
- Pham TĂ i
- Pham TĂ i
- Philippart François
- Picetti Edoardo
- Pierucci Elisabetta
- Piervincenzi E.
- Pinciroli Riccardo
- Pintado M.-C.
- Piquilloud Lise
- Piraino T.
- Piras ClĂĄudio
- Piras Stephanie
- Pirompanich P.
- Pisani Luigi
- Platas Enrique
- Plotnikow G.
- Porras Willy
- Porta Virginia
- Portilla M.
- Portugal J.
- Povoa Pedro R.
- Prat G.
- Pratto R.
- Preda Gabriel
- Prieto Isidro J.
- Prol-Silva E.
- Pugh Richard
- PĂ©rez-Araos Rodrigo A.
- Qi Yupeng
- Qian C.
- Qin Tiehe
- Qiu Haibo
- Qu Hongping
- Quintana T.
- Quispe Sierra Rosari
- Quispe Soto Rocio
- Rabbani R.
- Rabee Mohamed A.
- Rabie Ahmed
- Rahe Pereira M.A.
- Rai A.
- Raj Ashok Sundar
- Rajab Mostafa
- Ramdhani N.
- Ramey Elizabeth
- Ranieri V. Marco
- Rathod Darshana S.
- Ray B.
- Razek Assem Abdel
- Redwanul Huq S.M.
- Regli Adrian
- Reina Rosa
- Resano Sarmiento N.
- Reynaud Faustine
- Rialp G.
- Ricart P.
- Rice T.
- Richardson Angus
- Rieder M.
- Rinket M.
- Rios F.
- Risso Vazquez Alejandro
- Rittayamai N.
- Riva I.
- Rivette M.
- Roca O.
- Roche-Campo FerrĂĄn
- Rodriguez Gonzalez Daniel
- Rodriguez Tucto Xandra Yanina
- Rodriguez Covadonga
- Rodriguez G.
- Rogers A.
- Roldan R.
- Romano MarĂa Elena
- Rose Alastair
- Roux Damien
- Rouze A.
- Rubatto Birri Paolo Nahuel
- Ruilan Wang
- Ruiz Robledo A.
- Ruiz-Aguilar Antonio Luis
- RĂžrtveit Linda
- Sadahiro Tomohito
- Saez I.
- Sagardia Judith
- Saha R.
- Saha Rajnish
- Saiphoklang Narongkorn
- Saito Shigeki
- Salem Maie
- Sales Gabriele
- Salgado P.
- Samavedam Srinivas
- Sami Mebazaa Mhamed
- Samuelsson L.
- San Juan Roman Nandyelly
- Sanchez Patricia
- Sanchez-Ballesteros J.
- Sandoval Yazcitk
- Sani Emanuele
- Santos C.
- Santos M.
- Sanui Masamitsu
- Saravanabavan L.
- Sari S.
- Sarkany A.
- Sauneuf Bertrand
- Savioli M.
- Sazak H.
- Scano Riccardo
- Schneider F.
- Schortgen F.
- Schultz M.J.
- Schwarz Gabriele Leonie
- Seely A.
- Seiler Frederik
- Seker Tekdos Yasemin
- Seok Chan K.
- Serano Luca
- Serednicki Wojciech T.
- Serpa Neto Ary Serpa
- Setten M.
- Seçkin YĂŒcesoy Faruk
- Shah Asim
- Shah Bhagyesh K.
- Shang Y.
- Shanmugasundaram Pradeep
- Shapovalov K. G.
- Shebl E.
- Shiga T.
- Shime Nobuaki
- Shin P.
- Short J.
- Shuhua Chen
- Siddiqui Sughrat
- Silesky Jimenez J.I.
- Silva Sales Betania
- Silva Daniel
- Simons K.
- SjĂžbĂž Brit Ă .
- Slessor Dave
- Smiechowicz J.
- Smischney N.
- Smith M.
- Smith Paul J.
- Smith T.
- Snape S.
- Snyman Lindi
- Soetens F.
- Sook Hong K.
- Sosa Medellin M.Ă.
- Soto Giovanna Leonardo
- Souloy Xavier
- Sousa E.
- Sovatzis Stefania S.
- Sozutek Didem
- Spadaro S.
- Spagnoli M.
- Spittle N.
- Spivey M.
- SpÄngfors Martin
- Stapleton A.
- Stefanovic Branislava
- Stephenson Lorraine
- Stevenson E.
- Strand Kristian
- Strano Maria Teresa
- Straus S.
- Sun C.
- Sun Rongqing
- Sundaram V.
- Sunpark T.
- Surlemont E.
- Sutherasan Yuda
- Szabo Z.
- Szuldrzynski K.
- Tainter C.
- Takaba Akihiro
- Tallott Mandy
- Talmor D.
- Tamasato T.
- Tang Z.
- Tangsujaritvijit Viratch
- Taniguchi D.
- Taniguchi L.
- Tarantino F.
- Teerapuncharoen Krittika
- Temprano Susana
- Terragni P.
- Terzi Nicolas
- Thakur A.
- Theerawit P.
- Thille A.W.
- Thomas Matt J.C.
- Thungtitigul P.
- Thyrault M.
- Tilouch N.
- Timenetsky Karina Tavares
- Tirapu Juna
- Todeschini M.
- Tomas Roser
- Tomaszewski C.
- Tomescu D. Rodica
- Tonetti T.
- Tonnelier A.
- Trinder John T.
- Trongtrakul K.
- Truwit J.
- Tsuei B.
- Tulaimat Aiman
- Turan Sema
- Turkoglu M.
- Tyagi Sanjeev
- Ubeda Alejandro
- Vagginelli F.
- Valenti MarĂa Florencia
- Vallverdu Immaculada
- van Axel A.
- van den Hul I.
- van der Hoeven Hans G.
- van der Meer N.
- van Haren Frank M.P.
- Vanhoof M.
- Vargas-Ordoñez M.
- Vaschetto Rosanna R.
- Vascotto E.
- Vatsik M.
- Vaz Ana
- Vazquez-Sanchez Antonia
- Ventura S.
- Vermeijden J.W.
- Vidal Ănxela
- Vieira J.
- Vilela Costa Pinto B.
- Villagomez A.
- Villagra Ana
- Villegas Succar Cristina
- Vinorum O.G.
- Vitale G.
- Vj Ramesh
- Vochin Ana
- Voiriot G.
- Volta Carlo Alberto
- von Seth M.
- Wajdi Maazouzi Ahmed
- Walsh Don
- Wang S.
- Wardi G.
- Ween-Velken Nils Christian
- Wei Bilin
- Weller D.
- Welsh Deborah
- Welters Ingeborg Dorothea M.
- Wert M.
- Whiteley Simon M.
- Wilby E.
- Williams E.
- Williams K.
- Wilson Antoinette
- Wojtas Jadwiga
- Won Huh J.
- Wrathall David Wayne
- Wright Christopher
- Wu J.-F.
- Wunsch H.
- Xi Guo
- Xing Zhengjiang
- Xu H.
- Yamamoto K.
- Yan Jie
- Yang X.
- Yates E.
- Yazicioglu Mocin Ăzlem
- Ye Zhenglong
- Yildirim F.
- Yoshida Norifumi
- Yoshido Hector Higo Leon
- Young Lee B.
- Yu Gong
- Yu R.
- Yu T.
- Yuan B.
- Yuangtrakul N.
- Yumoto Tetsuya
- Yun Xie
- Yåñez J.
- Zakalik G.
- Zaki Ahmed
- Zalba-Etayo Begoña
- Zambon Massimo
- Zang Bin
- Zani G.
- Zarka Jonathan
- Zeggwagh A.A.
- Zerbi S.M.
- Zerman A.
- Zetterquist H.
- Zhang Guoxiu
- Zhang Hongwen
- Zhang Jiuzhi
- Zhang W.
- Zhang Weixin
- Zhao H.
- Zheng Jia
- Zhu Bin
- Zumaran R.
- Ăukurova Z.
- Ăveges NĂĄndo
- Publication venue
- Elsevier
- Publication date
- 01/01/2023
- Field of study
International audienceBackground: Current management practices and outcomes in weaning from invasive mechanical ventilation are poorly understood. We aimed to describe the epidemiology, management, timings, risk for failure, and outcomes of weaning in patients requiring at least 2 days of invasive mechanical ventilation. Methods: WEAN SAFE was an international, multicentre, prospective, observational cohort study done in 481 intensive care units in 50 countries. Eligible participants were older than 16 years, admitted to a participating intensive care unit, and receiving mechanical ventilation for 2 calendar days or longer. We defined weaning initiation as the first attempt to separate a patient from the ventilator, successful weaning as no reintubation or death within 7 days of extubation, and weaning eligibility criteria based on positive end-expiratory pressure, fractional concentration of oxygen in inspired air, and vasopressors. The primary outcome was the proportion of patients successfully weaned at 90 days. Key secondary outcomes included weaning duration, timing of weaning events, factors associated with weaning delay and weaning failure, and hospital outcomes. This study is registered with ClinicalTrials.gov, NCT03255109. Findings: Between Oct 4, 2017, and June 25, 2018, 10 232 patients were screened for eligibility, of whom 5869 were enrolled. 4523 (77·1%) patients underwent at least one separation attempt and 3817 (65·0%) patients were successfully weaned from ventilation at day 90. 237 (4·0%) patients were transferred before any separation attempt, 153 (2·6%) were transferred after at least one separation attempt and not successfully weaned, and 1662 (28·3%) died while invasively ventilated. The median time from fulfilling weaning eligibility criteria to first separation attempt was 1 day (IQR 0â4), and 1013 (22·4%) patients had a delay in initiating first separation of 5 or more days. Of the 4523 (77·1%) patients with separation attempts, 2927 (64·7%) had a short wean (â€1 day), 457 (10·1%) had intermediate weaning (2â6 days), 433 (9·6%) required prolonged weaning (â„7 days), and 706 (15·6%) had weaning failure. Higher sedation scores were independently associated with delayed initiation of weaning. Delayed initiation of weaning and higher sedation scores were independently associated with weaning failure. 1742 (31·8%) of 5479 patients died in the intensive care unit and 2095 (38·3%) of 5465 patients died in hospital. Interpretation: In critically ill patients receiving at least 2 days of invasive mechanical ventilation, only 65% were weaned at 90 days. A better understanding of factors that delay the weaning process, such as delays in weaning initiation or excessive sedation levels, might improve weaning success rates. Funding: European Society of Intensive Care Medicine, European Respiratory Society
Guidelines for the use and interpretation of assays for monitoring autophagy
- Author
- Abdalla Fabio C.
- Abeliovich Hagai
- Abraham Robert T.
- Acevedo-Arozena Abraham
- Adeli Khosrow
- Agholme Lotta
- Agnello Maria
- Agostinis Patrizia
- Aguirre-Ghiso Julio A.
- Ahn Hyung Jun
- Ait-Mohamed Ouardia
- Ait-Si-Ali Slimane
- Akematsu Takahiko
- Akira Shizuo
- Al-Younes Hesham M.
- Al-Zeer Munir A.
- Albert Matthew L.
- Albin Roger L.
- Alegre-Abarrategui Javier
- Aleo Maria Francesca
- Alirezaei Mehrdad
- Almasan Alexandru
- Almonte-Becerril Maylin
- Amano Atsuo
- Amaravadi Ravi K.
- Amarnath Shoba
- Amer Amal O.
- Anantharam Vellareddy
- Andrieu-Abadie Nathalie
- Ann David K.
- Anoopkumar-Dukie Shailendra
- Aoki Hiroshi
- Apostolova Nadezda
- Arancia Giuseppe
- Aris John P.
- Asanuma Katsuhiko
- Asare Nana Y.O.
- Ashida Hisashi
- Askanas Valerie
- Askew David S
- Auberger Patrick
- Baba Misuzu
- Backues Steven K.
- Baehrecke Eric H.
- Bahr Ben A.
- Bai Xue-Yuan
- Bailly Yannick
- Baiocchi Robert
- Baldini Giulia
- Balduini Walter
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- Bassham Diane C.
- Bast Robert C.
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- Wang Aimin
- Wang Chenguang
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- Wang Haichao
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- Xu Da-zhi
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- Yamamoto Akitsugu
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- Yan Xianghua
- Yanagida Mitsuhiro
- Yang Dun-Sheng
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- Yang Jin-Ming
- Yang Shi Yu
- Yang Wannian
- Yang Wei Yuan
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- Yao Tso-Pang
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- Yen Wei-Lien
- Yin Jia-jing
- Yin Xiao-Ming
- Yoo Ook-Joon
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- Yorimitsu Tomohiro
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- Yu Li
- Yu Long
- Yu Seong-Woon
- Yu Wai Haung
- Yuan Zhi-Min
- Yue Zhenyu
- Yun Cheol-Heui
- Yuzaki Michisuke
- Zabirnyk Olga
- Zacks David
- Zacksenhaus Eldad
- Zaffaroni Nadia
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- Zeitlin Scott O.
- Zhang Hong
- Zhang Hui-Ling
- Zhang Jianhua
- Zhang Jing-Pu
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- Zhang Lin
- Zhang Long
- Zhang Ming-Yong
- Zhang Xu Dong
- Zhao Mantong
- Zhao Yi-Fang
- Zhao Ying
- Zhao Zhizhuang J.
- Zheng Xiaoxiang
- Zhivotovsky Boris
- Zhong Qing
- Zhou Cong-Zhao
- Zhu Changlian
- Zhu Wei-Guo
- Zhu Xiao-Feng
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- Zhu Yuangang
- Zoladek Teresa
- Zong Wei-Xing
- Zorzano Antonio
- Zschocke JĂŒrgen
- Zuckerbraun Brian
- Publication venue
- Publication date
- 01/04/2012
- Field of study
Guidelines for the use and interpretation of assays for monitoring autophagy
- Author
- Abdalla Fabio C.
- Abeliovich Hagai
- Abraham Robert T.
- Acevedo-Arozena Abraham
- Adeli Khosrow
- Agholme Lotta
- Agnello Maria
- Agostinis Patrizia
- Aguirre-Ghiso Julio A.
- Ahn Hyung Jun
- Ait-Mohamed Ouardia
- Ait-Si-Ali Slimane
- Akematsu Takahiko
- Akira Shizuo
- Al-Younes Hesham M.
- Al-Zeer Munir A.
- Albert Matthew L.
- Albin Roger L.
- Alegre-Abarrategui Javier
- Aleo Maria Francesca
- Alirezaei Mehrdad
- Almasan Alexandru
- Almonte-Becerril Maylin
- Amano Atsuo
- Amaravadi Ravi K.
- Amarnath Shoba
- Amer Amal O.
- Anantharam Vellareddy
- Andrieu-Abadie Nathalie
- Ann David K.
- Anoopkumar-Dukie Shailendra
- Aoki Hiroshi
- Apostolova Nadezda
- Arancia Giuseppe
- Aris John P.
- Asanuma Katsuhiko
- Asare Nana Y.O.
- Ashida Hisashi
- Askanas Valerie
- Askew David S
- Auberger Patrick
- Baba Misuzu
- Backues Steven K.
- Baehrecke Eric H.
- Bahr Ben A.
- Bai Xue-Yuan
- Bailly Yannick
- Baiocchi Robert
- Baldini Giulia
- Balduini Walter
- Ballabio Andrea
- Bamber Bruce A.
- Bampton Edward T.W.
- Bartholomew Clinton R.
- Bassham Diane C.
- Bast Robert C.
- Batoko Henri
- Bay Boon-Huat
- Beau Isabelle
- Begley Thomas J.
- Behl Christian
- Behrends Christian
- Bekri Soumeya
- Bellaire Bryan
- Bendall Linda J.
- Benetti Luca
- Berliocchi Laura
- Bernardi Henri
- Bernassola Francesca
- Besteiro SĂ©bastien
- Bhatia-KiĆĄĆĄovĂĄ Ingrid
- Bi Xiaoning
- Biard-Piechaczyk Martine
- Blum Janice S.
- Boise Lawrence H.
- Bonaldo Paolo
- Boone David L
- Bornhauser Beat C.
- Bortoluci Karina R.
- Bossis Ioannis
- Bost Frédéric
- Bourquin Jean-Pierre
- Boya Patricia
- Boyer-Guittaut Michaël
- Bozhkov Peter V.
- Brady Nathan R
- Brancolini Claudio
- Brech Andreas
- Brenman Jay E.
- Brennand Ana
- Bresnick Emery H.
- Brest Patrick
- Bridges Dave
- Bristol Molly L.
- Brookes Paul S.
- Brown Eric J.
- Brumell John H.
- Brunetti-Pierri Nicola
- Brunk Ulf T.
- Bulman Dennis E.
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- Bultynck Geert
- Burbulla Lena F.
- Bursch Wilfried
- Butchar Jonathan P.
- Buzgariu Wanda
- Bydlowski Sergio P.
- BĂ©chet Daniel M.
- Cadwell Ken
- CahovĂĄ Monika
- Cai Dongsheng
- Cai Jiyang
- Cai Qian
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- Campanella Michelangelo
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- Candi Eleonora
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- Carra Serena
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- Casas Caty
- Castino Roberta
- Cebollero Eduardo
- Cecconi Francesco
- Celli Jean
- Chaachouay Hassan
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- Chai Chee-Yin
- Chan David C.
- Chan Edmond Y.
- Chang Raymond Chuen-Chung
- Che Chi-Ming
- Chen Ching-Chow
- Chen Guang-Chao
- Chen Guo-Qiang
- Chen Min
- Chen Quan
- Chen Steve S.-L.
- Chen WenLi
- Chen Xi
- Chen Xiangmei
- Chen Xiequn
- Chen Ye-Guang
- Chen Yingyu
- Chen Yongqiang
- Chen Yu-Jen
- Chen Zhixiang
- Cheng Alan
- Cheng Christopher H.K.
- Cheng Yan
- Cheong Heesun
- Cheong Jae-Ho
- Cherry Sara
- Chess-Williams Russ
- Cheung Zelda H.
- Chevet Eric
- Chiang Hui-Ling
- Chiarelli Roberto
- Chiba Tomoki
- Chin Lih-Shen
- Chiou Shih-Hwa
- Chisari Francis V.
- Cho Chi Hin
- Cho Dong-Hyung
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- Chu Charleen T
- Chuang Tsung-Hsien
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- Chun Taehoon
- Chwae Yong-Joon
- Chye Mee-Len
- Ciarcia Roberto
- Ciriolo Maria R.
- Clague Michael J.
- Clark Robert S.B.
- Clarke Peter G.H.
- Clarke Robert B
- Codogno Patrice
- Coller Hilary A.
- Colombo MarĂa I.
- Comincini Sergio
- Condello Maria
- Condorelli Fabrizio
- Cookson Mark R.
- Coombs Graham H
- Coppens Isabelle
- Corbalan Ramon
- Cossart Pascale
- Costelli Paola
- Costes Safia
- Coto-Montes Ana
- Couve Eduardo
- Coxon Fraser P.
- Cregg James M.
- Crespo José L.
- Cronjé Marianne J.
- Cuervo Ana Maria
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- Czaja Mark J.
- D'Amelio Marcello
- Darfeuille-Michaud Arlette
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- De Felici Massimo
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- de Haan Cornelis A.M.
- De Martino Luisa
- De Milito Angelo
- De Tata Vincenzo
- Debnath Jayanta
- Degterev Alexei
- Dehay Benjamin
- Delbridge Lea M.D.
- Demarchi Francesca
- Deng Yi Zhen
- Dengjel Jörn
- Dent Paul
- Denton Donna
- Deretic Vojo
- Desai Shyamal D.
- Devenish Rodney J.
- Di Gioacchino Mario
- Di Paolo Gilbert
- Di Pietro Chiara
- Diaz-Meco Maria T.
- Diaz-Nido Javier
- Dikic Ivan
- Dinesh-Kumar Savithramma P.
- Ding Wen-Xing
- Distelhorst Clark W.
- Diwan Abhinav
- Djavaheri-Mergny Mojgan
- Dokudovskaya Svetlana
- Dong Zheng
- Dorsey Frank C.
- Dosenko Victor
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- Dreux MarlĂšne
- Drew Mark E.
- Duan Qiuhong
- Duchosal Michel A
- Duff Karen E.
- Dugail Isabelle
- Durbeej Madeleine
- Duszenko Michael
- DĂaz-Araya Guillermo
- DĂaz-Laviada InĂ©s
- Edelstein Charles L.
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- Egea Gustavo
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- Elgendy Mohamed
- Ellerby Lisa M.
- Eng Kai Er
- Engelbrecht Anna-Mart
- Engelender Simone
- Erenpreisa Jekaterina
- Escalante Ricardo
- Esclatine Audrey
- Eskelinen Eeva-Liisa
- Espert Lucile
- Espina Virginia
- Fan Huizhou
- Fan Jia
- Fan Qi-Wen
- Fan Zhen
- Fang Shengyun
- Fang Yongqi
- Fanto Manolis
- Fanzani Alessandro
- Farkas Thomas
- Farre Jean-Claude
- Faure Mathias
- Fechheimer Marcus
- Feng Carl G.
- Feng Jian
- Feng Qili
- Feng Youji
- Feuer Ralph
- Figueiredo-Pereira Maria E.
- Fimia Gian Maria
- Fingar Diane C.
- Finkbeiner Steven
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- Finley Kim D.
- Fiorito Filomena
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- Flajolet Marc
- Flores Juan B. Kouri
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- Fortunato Franco
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- Fueyo Juan
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- Fukuda Mitsunori
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- Gaestel Matthias
- Gailly Philippe
- Gajewska Malgorzata
- Galliot Brigitte
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- Ganesh Subramaniam
- Ganetzky Barry
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- Gao Fen-Biao
- Gao George F.
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- Garcia Lorena
- Garcia-Manero Guillermo
- Garcia-Marcos Mikel
- Garmyn Marjan
- Gartel Andrei L.
- Gatti Evelina
- Gautel Mathias
- Gawriluk Thomas R.
- Gegg Matthew E.
- Geng Jiefei
- Germain Marc
- Gestwicki Jason E.
- Gewirtz David A
- Ghavami Saeid
- Ghosh Pradipta
- Giammarioli Anna M.
- Giatromanolaki Alexandra N.
- Gibson Spencer B.
- Gilkerson Robert W.
- Ginger Michael L.
- Ginsberg Henry N.
- Golab Jakub
- Goligorsky Michael S.
- Golstein Pierre
- Gomez-Manzano Candelaria
- Goncu Ebru
- Gongora CĂ©line
- Gonzalez Claudio D.
- Gonzalez Ramon
- Gonzalez-Rey Elena
- Gonzålez-Estévez Cristina
- GonzĂĄlez-Polo Rosa Ana
- Gorbunov Nikolai V.
- Gorski Sharon
- Goruppi Sandro
- Gottlieb Roberta A
- Gozuacik Devrim
- Granato Giovanna Elvira
- Grant Gary D.
- Green Kim N.
- Gregorc Ales
- Gros Frédéric
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- Grunt Thomas W.
- Gual Philippe
- Guan Jun-Lin
- Guan Kun-Liang
- Guichard Sylvie M.
- Gukovskaya Anna S.
- Gukovsky Ilya
- Gunst Jan
- Gustafsson Ă sa B.
- Halayko Andrew J.
- Hale Amber N.
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- Han Feng
- Han Ting
- Hancock Michael K.
- Hansen Malene
- Harada Hisashi
- Harada Masaru
- Hardt Stefan E.
- Harper J. Wade
- Harris Adrian L.
- Harris James
- Harris Steven D.
- Hashimoto Makoto
- Haspel Jeffrey A.
- Hayashi Shin-ichiro
- Hazelhurst Lori A.
- He Congcong
- He You-Wen
- Heidenreich Kim A.
- Helfrich Miep H.
- Helgason Gudmundur V.
- Henske Elizabeth P.
- Herman Brian
- Herman Paul K.
- Hetz Claudio
- Hilfiker Sabine
- Hill Joseph A
- Hocking Lynne J.
- Hofman Paul
- Hofmann Thomas G.
- Holyoake Tessa L.
- Hong Ming-Huang
- Hood David A.
- Hotamisligil Gökhan S.
- Houwerzijl Ewout J.
- Hu Bingren
- Hu Chien-an A.
- Hu Hong-Ming
- Hua Ya
- Huang Canhua
- Huang Ju
- Huang Shengbing
- Huang Wei-Pang
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- Hussain Sabah N.A.
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- Hwang Seungmin
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- Ilkhanizadeh Shirin
- Inoki Ken
- Into Takeshi
- Iovane Valentina
- Iovanna Juan L.
- Ip Nancy Y.
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- Jackson George R.
- Jackson William T.
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- Jiang Hongchi
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- Jiang Ming
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- Jiménez Alberto
- Jin Meiyan
- Jin Shengkan V.
- Joe Cheol O.
- Johansen Terje
- Johnson Daniel E.
- Johnson Gail V.W.
- Jones Nicola L.
- Joseph Bertrand
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- Joubert Annie M.
- JuhĂĄsz GĂĄbor
- Juillerat-Jeanneret Lucienne
- Jung Chang Hwa
- Jung Yong-Keun
- JÀÀttelÀ Marja
- Kaarniranta Kai
- Kaasik Allen
- Kabuta Tomohiro
- Kadowaki Motoni
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- Yang Dun-Sheng
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- Yang Jin-Ming
- Yang Shi Yu
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- Yang Wei Yuan
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- Yao Meng-Chao
- Yao Tso-Pang
- Yeganeh Behzad
- Yen Wei-Lien
- Yin Jia-jing
- Yin Xiao-Ming
- Yoo Ook-Joon
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- Yorimitsu Tomohiro
- Yoshikawa Yuko
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- You Ho Jin
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- Younes Anas
- Yu Li
- Yu Long
- Yu Seong-Woon
- Yu Wai Haung
- Yuan Zhi-Min
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- Zabirnyk Olga
- Zacks David
- Zacksenhaus Eldad
- Zaffaroni Nadia
- Zakeri Zahra
- Zeh III Herbert J.
- Zeitlin Scott O.
- Zhang Hong
- Zhang Hui-Ling
- Zhang Jianhua
- Zhang Jing-Pu
- Zhang Jue
- Zhang Lin
- Zhang Long
- Zhang Ming-Yong
- Zhang Xu Dong
- Zhao Mantong
- Zhao Yi-Fang
- Zhao Ying
- Zhao Zhizhuang J.
- Zheng Xiaoxiang
- Zhivotovsky Boris
- Zhong Qing
- Zhou Cong-Zhao
- Zhu Changlian
- Zhu Wei-Guo
- Zhu Xiao-Feng
- Zhu Xiongwei
- Zhu Yuangang
- Zoladek Teresa
- Zong Wei-Xing
- Zorzano Antonio
- Zschocke JĂŒrgen
- Zuckerbraun Brian
- Publication venue
- Publication date
- 01/04/2012
- Field of study
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field
Guidelines for the use and interpretation of assays for monitoring autophagy
- Author
- Abdelhaq Rami
- Abhinav Diwan
- Abraham Acevedo-Arozena
- Adalberto Merighi
- Adi Kimchi
- Adrian L. Harris
- Agnieszka Sirko
- Ahlberg J
- Ai Yamamoto
- Aimable Nahimana
- Aimee L. Edinger
- Aimin Wang
- Akiko Iwasaki
- Akio Kihara
- Akitsugu Yamamoto
- Aksam EB
- Alan Cheng
- Albert R. La Spada
- Alberto Jiménez
- Alec C. Kimmelman
- Ales Gregorc
- Alessandro Fanzani
- Alexander J. Whitworth
- Alexandra N. Giatromanolaki
- Alexandru Almasan
- Alexei Degterev
- Alexei Terman
- Alfred J. Meijer
- Alicia Meléndez
- Alison Simmons
- Allan B. Caplan
- Allan Tsung
- Allen Kaasik
- Alvin J.X. Lee
- Amal O. Amer
- Amber N. Hale
- Ameeta Kelekar
- Amy A. Kiger
- Ana Brennand
- Ana Coto-Montes
- Ana Maria Cuervo
- Ana Milojkovic
- Anas Younes
- Anastasis Stephanou
- Anders H. Lund
- Andrea Ballabio
- Andreas Brech
- Andreas Mayer
- Andreas S. Reichert
- Andreas Wyttenbach
- Andrei L. Gartel
- Andrew J. Halayko
- Andrew Lieberman
- Andrew Thorburn
- Andriy A. Sibirny
- Andriy Nemchenko
- Angelika A. Noegel
- Angelo De Milito
- Angelo Poletti
- Anil K. Sood
- Anil Prasad
- Ann Progulske-Fox
- Anna Katharina Simon
- Anna M. Giammarioli
- Anna M. Krichevsky
- Anna Nogalska
- Anna S. Gukovskaya
- Anna-Mart Engelbrecht
- Anne RĂ©gnier-Vigouroux
- Anne Simonsen
- Annie M. Joubert
- Anthony H.V. Schapira
- Anthony M.J. Sanchez
- Antoinette Lemoine
- Antonio MusarĂČ
- Antonio Piras
- Antonio Zorzano
- Anumantha G. Kanthasamy
- Arlette Darfeuille-Michaud
- Arthi Kanthasamy
- Ashok Kumar
- Assaf Rudich
- Atsuhiro Ichihara
- Atsuo Amano
- Attila L. KovĂĄcs
- Audrey Esclatine
- Augustine M.K. Choi
- Ausra Sasnauskiene
- Aviva M. Tolkovsky
- Baharia Mograbi
- Bakhos A. Tannous
- Barry Ganetzky
- Bassam Janji
- Beat C. Bornhauser
- Beat Nyfeler
- Beata Sikorska
- Behzad Yeganeh
- Ben A. Bahr
- Ben C.B. Ko
- Benjamin Dehay
- Benjamin Loos
- Berger B
- Bertrand Joseph
- Bertrand Mollereau
- Beth Levine
- BethAnn McLaughlin
- Biederbick A
- Binfeng Lu
- Bingren Hu
- Bingwei Lu
- Biolo G
- Birmingham CL
- Birmingham CL
- Björn Stork
- BjĂžrkĂžy G
- Bo Liu
- Bo Lu
- Boon-Huat Bay
- Boris Turk
- Boris Zhivotovsky
- Bradly G. Wouters
- Brian Herman
- Brian P. Rubin
- Brian Zuckerbraun
- Brigitte Galliot
- Brinda Ravikumar
- Brown CR
- Bruce A. Bamber
- Bruce H. Reed
- Bruno Calabretta
- Bryan Bellaire
- Bulent Ozpolat
- Byung-Wan Lee
- BĂ€rbel Rohrer
- BĂ©langer M
- Cam Patterson
- Camilla Palumbo
- Camille Martinand-Mari
- Candelaria Gomez-Manzano
- Canhua Huang
- Cantino D
- Carine Michiels
- Carl G. Feng
- Carlos LĂłpez-OtĂn
- Carlos S. Subauste
- Carmine Settembre
- Carol A. Mercer
- Carole Kretz-Remy
- Caroline A. Whitehouse
- Catherine McDermott
- Cathleen R. Carlin
- Cathy Tournier
- Caty Casas
- Chan EY
- Chang Hwa Jung
- Changlian Zhu
- Chanhee Kang
- Chao-Yu Miao
- Charbel E.-H. Moussa
- Charleen T. Chu
- Charles Grose
- Charles L. Edelstein
- Charles Swanton
- Chee-Yin Chai
- Chenghan Wu
- Chenguang Wang
- Chengyu Liang
- Cheol O. Joe
- Cheol-Heui Yun
- Chera S
- Cheryl L. Walker
- Chi Hin Cho
- Chi-Ming Che
- Chia-Yi Kuan
- Chiara Di Pietro
- Chien-an A. Hu
- Chihiro Sasakawa
- Ching-Chow Chen
- Chiou-Feng Lin
- Christiaan Leeuwenburgh
- Christian Behl
- Christian Behrends
- Christian G. Meyer
- Christian MĂŒnz
- Christian PoĂŒs
- Christian Sell
- Christine McDonald
- Christoph SchĂŒller
- Christopher H.K. Cheng
- Christopher J. Rhodes
- Christopher R. McMaster
- Chun-Feng Liu
- Chun-Nam Lok
- Chwen-Ming Shih
- Ciechomska IA
- Cindy K. Miranti
- Cinthya Sternberg
- Ciro Isidoro
- Clarissa von Haefen
- Clark W. Distelhorst
- Claudia D. Spies
- Claudine Kraft
- Claudio Brancolini
- Claudio D. Gonzalez
- Claudio Hetz
- Claudio Schneider
- Claus Kerkhoff
- Clelia Miracco
- Clinton R. Bartholomew
- Colombo MI
- Cong-Zhao Zhou
- Congcong He
- Conrad C. Weihl
- Corey Nislow
- Cornelis A.M. de Haan
- Craig B. Thompson
- Craig M. Walsh
- Cristiano Simone
- Cristina Gonzålez-Estévez
- Cuervo AM
- CĂ©cile Vindis
- CĂ©line Gongora
- Da-zhi Xu
- Dagda RK
- Dalibor Mijaljica
- Daniel E. Johnson
- Daniel F. Schorderet
- Daniel H. Fowler
- Daniel J. Klionsky
- Daniel J. Ledbetter
- Daniel M. BĂ©chet
- Daniela Marazziti
- Dao-Cheng Wu
- Daolin Tang
- Daotai Nie
- Dave Bridges
- David A. Gewirtz
- David A. Hood
- David A. Leib
- David A. Sinclair
- David C. Chan
- David C. Rubinsztein
- David H. Kessel
- David H. Perlmutter
- David J. McConkey
- David K. Ann
- David L. Boone
- David M. Sabatini
- David S. Askew
- David Sulzer
- David Zacks
- Davide Malagoli
- Dawei Wang
- Debasish Sinha
- Denne SC
- Dennis E. Bulman
- Deok Ryong Kim
- Derek P. Narendra
- Derick G. Wansink
- Devrim Gozuacik
- Diane C. Bassham
- Diane C. Fingar
- Diane E. Merry
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- Virginia Espina
- Virginie Carmignac
- Vitaliy O. Kaminskyy
- Vivek K. Unni
- Vladimir Kirkin
- Vladimir P. Skulachev
- Vladimir Trajkovic
- Vojo Deretic
- Vojtech Skop
- vom Dahl S
- vom Dahl S
- von Haefen C
- Wafik S. El-Deiry
- Wai Haung Yu
- Walter Balduini
- Walter Malorni
- Walter T. Klimecki
- Wan-Wan Lin
- Wanda Buzgariu
- Wannian Yang
- Weber SM
- Webster P
- Weei-Chin Lin
- Wei Liu
- Wei Y
- Wei Yuan Yang
- Wei-Chiang Shen
- Wei-Guo Zhu
- Wei-Jia Kong
- Wei-Lien Yen
- Wei-Pang Huang
- Wei-Xing Zong
- Weidong Le
- Weijie Li
- Wen-Xing Ding
- WenLi Chen
- Wiep Scheper
- Wilfried Bursch
- William A. Weiss
- William K.K. Wu
- William T. Jackson
- Wim Martinet
- Wim P. Vandenberghe
- Winter G
- Wolfdieter Springer
- Won-Ki Huh
- Wouter G. van Doorn
- Xavier Molero
- Xi Chen
- Xiang Mao
- Xiang-Bo Wan
- Xianghua Yan
- Xiangmei Chen
- Xiao-Feng Zhu
- Xiao-Ming Yin
- Xiaolei Xu
- Xiaoning Bi
- Xiaoxiang Zheng
- Xiequn Chen
- Xinbing Sui
- Xinglong Wang
- Xiongwei Zhu
- Xiujie J. Wang
- Xu Dong Zhang
- Xue-Jun Li
- Xue-Yuan Bai
- Xuejun Jiang
- Xuejun Jiang
- Xueqin Song
- Ya Hua
- Yaichiro Kotake
- Yan Cheng
- Yan-Bo Li
- Yang Shi
- Yannick Bailly
- Yano K
- Yasuhiko Tomino
- Yasuo Uchiyama
- Yasuyoshi Sakai
- Ye-Guang Chen
- Yi Zhen Deng
- Yi-Fang Zhao
- Yi-Ling Lin
- Yi-Ping Li
- Ying Zhao
- Ying-Jan Wang
- Yingyu Chen
- Yipeng Wang
- Yohta Shimada
- Yokota S
- Yong Ma
- Yong-Joon Chwae
- Yong-Keun Jung
- Yong-Sun Kim
- Yonghyun Kim
- Yongqi Fang
- Yongqiang Chen
- Yoon-A Kang
- Yoshiaki Kamada
- Yoshihiro Shidoji
- Yoshimori T
- Yoshimura K
- Yoshinori Kawazoe
- Yoshitaka Isaka
- Yoshitaka Takano
- Yotaro Izumi
- You-Wen He
- Youji Feng
- Young Ho Koh
- Young-Chau Liu
- Yu-Jen Chen
- Yuan Tian
- Yuangang Zhu
- Yuji Moriyasu
- Yuko Yoshikawa
- Yule Liu
- Yung-Feng Liao
- Yves L. Marcel
- Zahra Zakeri
- Zelda H. Cheung
- Zhang H
- Zhen Fan
- Zhen Lu
- Zhen-Bo Wang
- Zheng Dong
- Zheng-Hong Qin
- Zhenyu Yue
- Zhi-Jun Sun
- Zhi-Min Yuan
- Zhifen Yang
- Zhigang Charles Wang
- Zhijun Xi
- Zhinong Wang
- Zhiping Xie
- Zhixiang Chen
- Zhizhuang J. Zhao
- Zhonglin Xie
- Zhou G
- Zhu H
- Zixu Mao
- Zu-Hang Sheng
- Zuzana Papackova
- Zvulun Elazar
- Ă sa B. Gustafsson
- Ăverbye A
- Publication venue
- 'Informa UK Limited'
- Publication date
- 01/01/2012
- Field of study
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field
Elective Cancer Surgery in COVID-19âFree Surgical Pathways During the SARS-CoV-2 Pandemic: An International, Multicenter, Comparative Cohort Study
- Author
- Aawsaj Y
- Abad Gurumeta A
- Abad-Motos A
- Abate E
- Abatini C
- Abbadessa F
- Abbas AM
- Abbassi-Ghadi N
- Abbott D
- Abbott T
- Abbott Tom EF
- Abd Elazeem HAS
- Abdalla M
- Abdallah M
- Abdel Al S
- Abdel Jalil R
- Abdeldayem H
- Abdelkader Salama I
- Abdelkarem MM
- Abdelkarim M
- Abdelmajeed A
- Abdelrahman A
- Abdelrahman S
- Abdelsamed A
- Abdou M
- Abdulkareem A
- Abdulwahed E
- Abdur-Rahman L
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- AbellĂĄn M
- Aboelkassem Ibrahim A
- Abosamak NE
- Abou Chaar MK
- Abou Chaar Mohammad K.
- Abou-Khalil J
- Abouassi A
- Aboulkassem H
- Abreu da Silva A
- Abu Zaânouneh FJ
- Abu J
- Abu-Nayla I
- Abubakar A
- Abukhalaf Sadi
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- Achalandabaso Boira M
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- Acrich E
- Adamina M
- Adamina Michel
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- Addae-Boateng E
- Ademuyiwa Adesoji O
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- Adeyeye A
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- Agarwal Arnav
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- Agresta F
- Aguado H
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- Ahad S
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- Ahmeidat A
- Ahn JH
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- Aimé A
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- on behalf of the COVIDSurg Collaborative
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- Tartaglia D
- Tasis N
- Taskiran C
- Tatar OC
- Tatsis D
- Tavares F
- Tayeh S
- Taylor Elliott H
- Tebala GD
- Tedde A
- Tedde M
- Teh C
- Teh E
- Teixeira J
- Teixeira M
- Tejero-Pintor FJ
- Tenreiro N
- Teoh LY
- Tepetes K
- Tercero-Uribe AI
- Terra RM
- Tesei M
- Testa V
- Testelin S
- Teves M
- Tewari N
- Tezas S
- Thaha M
- Thakur B
- Tham D
- Thanapal MR
- Thekinkattil Dinesh
- Thekkinkattil D
- Theodosopoulos T
- Theophilidou E
- Theophilou G
- Thiruchelvam J
- Thole S
- Thomas A
- Thomas E
- Thomas M
- Thomas R
- Thompson A
- Thompson D
- Thornhill M
- Thrumurthy S
- Thulasiraman SV
- Tilaveridis I
- Timbrell S
- Timon C
- Tingle S
- Tirloni L
- Tirotta F
- Tirotta Fabio
- Titu S
- Tiwari Abhinav
- Todorovic M
- Toia F
- Tojal A
- Tolani MA
- Tonini V
- Toogood G
- Tooulias A
- Topczewski TE
- Torales J
- Tormey S
- Torne A
- Tornese S
- Torné R
- Torres Arcos C
- Torres A
- Torretta S
- Townend D
- Townend P
- Townend Philip
- Townson A
- Trabulsi N
- Travaglio E
- Tribillon E
- Trivic A
- Troncoso Pereira P
- Tropeano FP
- Trout Isobel M
- Truant S
- Trujillo DĂaz J
- Tsai L
- Tsaousis V
- Tsarkov P
- Tsiamalou I
- Tsiaoussis J
- Tsibulak I
- Tsirlis T
- Tsolakidis D
- Tsoulfas G
- Tsoulfas Georgios
- Tucci R
- Tudela M
- Tudisco R
- Tufo A
- Tugmen C
- Tulina I
- Tuncer K
- Turkington R
- Turnage K
- Turnbull A
- Turrado-Rodriguez V
- Turri G
- Tustin H
- Tutoveanu G
- Tuveri M
- Tweedle E
- Tzedakis S
- Tzerbinis H
- Tzovaras G
- Ugarte-Sierra B
- UludaÄ SS
- Umair M
- Umar M
- Underwood C
- Uno M
- Uras C
- Urbani A
- Urbieta A
- Usai A
- Usandivaras M
- Uslu G
- Uson-Bouthelier T
- Usseglio J
- Utkan NZ
- Uyanik MS
- Uzunoglu FG
- Vaccarella G
- Vahtsevanos K
- Valbuena Jabares V
- Valdes-Hernandez J
- Valente da Costa A
- Valero Soriano M
- Valero R
- Vallance A
- Vallance Abigail
- Valle Rubio A
- Valtzoglou V
- Valverde S
- Van den Eynde J
- Van den Eynde R
- van der Plas Willemijn
- Van Duijvendijk P
- Van Heinsbergen M
- Van Houdt W
- van Ramshorst Gabrielle H
- Van Wyngaard T
- Vanounou T
- Varazzani A
- Vargas L
- Varley I
- Varma M
- VasljeviÄ J
- Vassiliu P
- Vatansever D
- Vatish J
- Vatish M
- Vaz Pereira R
- Vaz OP
- Vaz S
- Vazquez fernandez A
- Veiga D
- Velagapudi S
- Velchuru V
- Velickovic D
- Velidedeoglu M
- Venetis G
- Venn M
- Venn Mary L
- Venn Mary L.
- Venskutonis D
- Vera Mansilla C
- Verberne C
- Vergari R
- Vernon L
- Vervoort D
- Vescio G
- Vezakis A
- Vidakovic S
- Videira JF
- Videria Jose Flavio
- Vidya R
- Vidya Raghavan
- Vieira Caroço T
- Vieira Paiva Lopes AC
- Vieira A
- Vieira B
- Vieira P
- Vignali A
- Vigorita V
- Vijayan D
- Vilatoba M
- Vilendecic Z
- Villalabeitia Ateca I
- Vimalachandran D
- Vimalachandran Dale
- Vimalchandran Dale
- Vinayagam R
- Violante T
- Visgauss J
- Viswambaram P
- Viswanath Y
- Vitali A
- Vitone L
- Vives RV
- Vo UG
- Vogel J
- Vollkommer T
- Von Papen M
- Vovola F
- Vucic N
- Vujanac V
- Wade R
- Wadham B
- Waheed S
- Wai K
- Wali A
- Walker C
- Walker D
- Walkes K
- Wallwork K
- Walton G
- Walton T
- Waqar SH
- Warburton H
- Ward D
- Ward S
- Waris Farooka M
- Warren O
- Warren Oliver J
- Waseem S
- Wasim T
- Watson D
- Watson LJ
- Watson N
- Weaver H
- Webber L
- Wedderburn Duane
- Weitz J
- Welsch T
- Wensley F
- Werner J
- West C
- West D
- Westwood E
- Whelan M
- Whelan P
- Whisker L
- Whitehall E
- Whitmore H
- Wilkin R
- Wilkin Richard JW
- Wilkins A
- Wilkins Alex
- Williams G
- Williams J
- Williams K
- Williams MA
- Williams T
- Williamson K
- Wilson M
- Wimberger P
- Wimmer A
- Winnand P
- Winter D
- Winter S
- Winter Stuart C
- Wolfer S
- Wong MPK
- Wong S
- Wong WJ
- Woodham A
- Woodun H
- Worku D
- Wright G
- Wright Naomi
- Wullschleger M
- Wustrack R
- Xenaki S
- Xu MJ
- Xyda SE
- Xynos E
- Yahaya AS
- Yang W
- Yassin N
- Ye G
- Yesantharao P
- Yetkin G
- YeĆilyurt D
- Yiallourou A
- Yıldırım A
- Yildiz A
- Ying Y
- Yip V
- Yordanov S
- Young R
- Yousof EA
- Youssef A
- Youssef M
- Yurttas C
- YĂĄnez C
- YĂŒksel E
- Zacharoulis D
- Zafar SN
- Zakai D
- Zakaria AD
- Zakaria R
- Zakaria Z
- Zambelli MF
- Zambon M
- Zamora L
- Zamvar V
- Zani S
- Zanotti S
- Zanus G
- Zapardiel I
- Zarabini AG
- Zegarac M
- ZeliÄ M
- Zeller N
- Zengin AK
- Zerbib P
- Zervas K
- Zese M
- Zhang H
- Zivanovic M
- Zivkovic M
- Zizzo M
- Zografos C
- Zonta S
- Zorrilla OrtĂșzar J
- Zourntou S
- Zouzoulas D
- Zubaidi A
- Zullo A
- Ăngelo M
- ĂlgĂ„ A
- ĂapoÄlu R
- Ăelebi F
- Ăolak E
- Ăfner D
- Ăhlberger L
- ĂÄĂŒcĂŒ H
- ĂberrĂŒck L
- Äoza I
- Ć imko K
- Ć ÄepanoviÄ V
- ĆœateckĂœ J
- ĐĐ°ĐŒĐ°Đ»ĐŸĐČ Đ
- Publication venue
- American Society of Clinical Oncology
- Publication date
- 01/01/2021
- Field of study