766 research outputs found
Hydrographic observations from the US/PRC Cooperative Program in the Western Equatorial Pacific Ocean, cruises 5-8
In support of the Tropical Ocean and Global Atmosphere (TOGA) program, investigators from the Woods Hole Oceanographic Institution (WHOI), NOAA Pacific Marine Environmental Laboratory (PMEL), and the State Oceanic Administration (SOA) from both Qingdao (First Institute) and Guangzhou (South China Sea Branch) conducted hydrographic observations
aboard the Chinese R/V Xiang Yang Hong 14 in the western equatorial Pacific Ocean. The objective of this component of the TOGA
program was to document the water mass property distributions of the western equatorial Pacific and describe the oceanic velocity field. The four cruises summarized here were conducted during the period November 1988 to July 1990 and are the final half of an eight cruise repeated survey of the region begun in 1985. Conductivity-Temperature-Depth-Oxygen (CTD/O2) stations were collected
to a minimum cast depth of 2500m or the bottom when shallower. The cruises reoccupied the same stations to provide temporal information. Summarized listings of CTD/02 data together with selected physical properties of sea water for these cruises
are provided here, as well as a description of the hardware used and an explanation of the data reduction techniques employed.Funding was provided by the National Oceanic and Atmospheric Administration
under Grant No. NA85AA-D-AC117
Characterization and Prevalence of a New Porcine Calicivirus in Swine, United States
Real-time reverse transcription PCR revealed that new St-Valerien–like porcine caliciviruses are prevalent (2.6%–80%; 23.8% overall) in finisher pigs in North Carolina. One strain, NC-WGP93C, shares 89.3%–89.7% genomic nucleotide identity with Canadian strains. Whether these viruses cause disease in pigs or humans or are of food safety concern requires further investigation
Hydrographic observations from the US/PRC Cooperative Program in the Western Equatorial Pacific Ocean, cruises 1-4
In support of the Tropical Oceans and Global Atmosphere (TOGA) program, investigators from Woods Hole Oceanographic
Institution (WHOI), NOAA Pacific Marine Envionmental Laboratory and the State Oceanic Administration (SOA) from both
Qingdao (First Institute) and Guangzhou (South China Sea Branch) conducted hydrographic observations aboard the Chinese
Research vessels Xiang Yang Hong 5 and Xiang Yang Hong 14 in the western equatorial Pacific. The objective of this component of
the TOGA program was to document the water mass property distributions of the western equatorial Pacific Ocean and describe the
oceanic velocity field. The four cruises summarized here were conducted during the period November 1985 to June 1988 and are
the first half of an eight cruise repeated survey of the region scheduled to be completed in spring 1990. Conductivity-Temperatue-Depth-Oxygen (CTD/02) stations were collected to a minimum cast depth of 2,500 m or the bottom when shallower. The cruises reoccupied the same stations to provide temporal information. Summarized listings of CTD/O2 data together with selected physical
properties of sea water for these cruises are provided here, as well as a description of the hardware used and an explanation of the
data reduction tehniques employed.Funding was provided by the National Oceanic and Atmospheric Administration
Genetic mapping in mice identifies DMBT1 as a candidate modifier of mammary tumors and breast cancer risk
Low-penetrance breast cancer susceptibility alleles seem to play a significant role in breast cancer risk but are difficult to identify in human cohorts. A genetic screen of 176 N2 backcross progeny of two Trp53+/- strains, BALB/c and C57BL/6, which differ in their susceptibility to mammary tumors, identified a modifier of mammary tumor susceptibility in an ∼25-Mb interval on mouse chromosome 7 (designated SuprMam1). Relative to heterozygotes, homozygosity for BALB/c alleles of SuprMam1 significantly decreased mammary tumor latency from 70.7 to 61.1 weeks and increased risk twofold (P = 0.002). Dmbt1 (deleted in malignant brain tumors 1) was identified as a candidate modifier gene within the SuprMam1 interval because it was differentially expressed in mammary tissues from BALB/c-Trp53+/- and C57BL/6-Trp53+/- mice. Dmbt1 mRNA and protein was reduced in mammary glands of the susceptible BALB/c mice. Immunohistochemical staining demonstrated that DMBT1 protein expression was also significandy reduced in normal breast tissue from women with breast cancer (staining score, 1.8; n = 46) compared with cancer-free controls (staining score, 3.9; n = 53; P < 0.0001). These experiments demonstrate the use of Trp53+/- mice as a sensitized background to screen for low-penetrance modifiers of cancer. The results identify a novel mammary tumor susceptibility locus in mice and support a role for DMBT1 in suppression of mammary tumors in both mice and women
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HER-2-Targeted Nanoparticle-Affibody Bioconjugates for Cancer Therapy
Affibodies are a class of polypeptide ligands that are potential candidates for cell- or tissue-specific targeting of drug-encapsulated controlled release polymeric nanoparticles (NPs). Here we report the development of drug delivery vehicles comprised of polymeric NPs that are surface modified with Affibody ligands that bind to the extracellular domain of the trans-membrane human epidermal growth factor receptor 2 (HER-2) for targeted delivery to cells which over express the HER-2 antigen. NPs lacking the anti-HER-2 Affibody did not show significant uptake by these cells. Using paclitaxel encapsulated NP-Affibody (1 wt% drug loading), we demonstrated increased cytotoxicity of these bioconjugates in SK-BR-3 and SKOV-3 cell lines. These targeted, drug encapsulated NPAffibody bioconjugates may be efficacious in treating HER-2 expressing carcinoma
The ocean sampling day consortium
Ocean Sampling Day was initiated by the EU-funded Micro B3 (Marine Microbial Biodiversity, Bioinformatics, Biotechnology) project to obtain a snapshot of the marine microbial biodiversity and function of the world’s oceans. It is a simultaneous global mega-sequencing campaign aiming to generate the largest standardized microbial data set in a single day. This will be achievable only through the coordinated efforts of an Ocean Sampling Day Consortium, supportive partnerships and networks between sites. This commentary outlines the establishment, function and aims of the Consortium and describes our vision for a sustainable study of marine microbial communities and their embedded functional traits
Data-driven approach for creating synthetic electronic medical records
<p>Abstract</p> <p>Background</p> <p>New algorithms for disease outbreak detection are being developed to take advantage of full electronic medical records (EMRs) that contain a wealth of patient information. However, due to privacy concerns, even anonymized EMRs cannot be shared among researchers, resulting in great difficulty in comparing the effectiveness of these algorithms. To bridge the gap between novel bio-surveillance algorithms operating on full EMRs and the lack of non-identifiable EMR data, a method for generating complete and synthetic EMRs was developed.</p> <p>Methods</p> <p>This paper describes a novel methodology for generating complete synthetic EMRs both for an outbreak illness of interest (tularemia) and for background records. The method developed has three major steps: 1) synthetic patient identity and basic information generation; 2) identification of care patterns that the synthetic patients would receive based on the information present in real EMR data for similar health problems; 3) adaptation of these care patterns to the synthetic patient population.</p> <p>Results</p> <p>We generated EMRs, including visit records, clinical activity, laboratory orders/results and radiology orders/results for 203 synthetic tularemia outbreak patients. Validation of the records by a medical expert revealed problems in 19% of the records; these were subsequently corrected. We also generated background EMRs for over 3000 patients in the 4-11 yr age group. Validation of those records by a medical expert revealed problems in fewer than 3% of these background patient EMRs and the errors were subsequently rectified.</p> <p>Conclusions</p> <p>A data-driven method was developed for generating fully synthetic EMRs. The method is general and can be applied to any data set that has similar data elements (such as laboratory and radiology orders and results, clinical activity, prescription orders). The pilot synthetic outbreak records were for tularemia but our approach may be adapted to other infectious diseases. The pilot synthetic background records were in the 4-11 year old age group. The adaptations that must be made to the algorithms to produce synthetic background EMRs for other age groups are indicated.</p
Knockdown of CypA inhibits interleukin-8 (IL-8) and IL-8-mediated proliferation and tumor growth of glioblastoma cells through down-regulated NF-κB
Although cyclophilin A (CypA) has been reported to be over-expressed in cancer cells and solid tumors, its expression and role in glioblastomas have not been studied. Herein, we show that expression of CypA in human glioblastoma cell lines and tissues is significantly higher than in normal human astrocytes and normal counterparts of brain tissue. To determine the role of over-expressed CypA in glioblastoma, stable RNA interference (RNAi)-mediated knockdown of CypA (CypA KD) was performed in gliobastoma cell line U87vIII (U87MG · ΔEGFR). CypA KD stable single clones decrease proliferation, infiltration, migration, and anchorage-independent growth in vitro and with slower growth in vivo as xenografts in immunodeficient nude mice. We have also observed that knockdown of CypA inhibits expression of interleukin-8 (IL-8), a tumorigenic and proangiogenic cytokine. Conversely, enforced expression of CypA in the CypA KD cell line, Ud-12, markedly enhanced IL-8 transcripts and restored Ud-12 proliferation, suggesting that CypA-mediated IL-8 production provides a growth advantage to glioblastoma cells. CypA knockdown-mediated inhibition of IL-8 is due to reduced activity of NF-κB, which is one of the major transcription factors regulating IL-8 expression. These results not only establish the relevance of CypA to glioblastoma growth in vitro and in vivo, but also suggest that small interfering RNA-based CypA knockdown could be an effective therapeutic approach against glioblastomas
The state of the Martian climate
60°N was +2.0°C, relative to the 1981–2010 average value (Fig. 5.1). This marks a new high for the record. The average annual surface air temperature (SAT) anomaly for 2016 for land stations north of starting in 1900, and is a significant increase over the previous highest value of +1.2°C, which was observed in 2007, 2011, and 2015. Average global annual temperatures also showed record values in 2015 and 2016. Currently, the Arctic is warming at more than twice the rate of lower latitudes
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