Adherence of hip and knee arthroplasty studies to RSA standardization guidelines

Peer reviewe

Identification and characterization of acidity-tolerant and aluminum-resistant bacterium isolated from tea soil

An acidity-tolerant, aluminum resistant bacterium was isolated from tea soils in Kagoshima Experimental Station (Japan). Based on the morphological, physiological and biochemical characteristics and 16S rDNA nucleotide sequence analysis, the bacterium was identified as Bacillus sp. An 3 (DQ234657) in Bacillus cereus group. The bacterium was able to grow on S-LB plates (pH 3.7) with 1.0 g/L Al and survived in LB broth even at 10 g/L Al (pH 2.0). While cultured, the growth of the bacterial strain in LB liquid medium containing increasing concentrations of Al (0, 100 and 200 ppm), was inhibited by the presence of Al, especially at concentration of 200 ppm. The pH of culture medium without Al increased steeply and reached pH 7.0 after 10 days, meanwhile it was almost constant in the other cases. The elimination of Al from culture medium by the bacterium was also affirmed and it was more conspicuous at 100 ppm Al. Due to their tolerance to high acidity, resistance to and removal of a substantial amount of Al, the bacterium might be applicable in restoring acidic soils, particularly acidified tea garden soils.Keywords: Tea garden soil, acidity-tolerant bacterium, aluminum-resistant bacteriumAfrican Journal of Biotechnology, Vol 13(27) 2715-272

Novel in vitro and in silico insights of the multi-biological activities and chemical composition of Bidens tripartita L.

Bidens tripartita L. is a traditional phyto-remedy used in several countries, yet there is still a paucity of data on its biological potential. We aimed to provide new insights on the pharmacological potential of extracts prepared from B. tripartita via highlighting its antioxidant, key enzymes inhibitory potency, and DNA protecting effects. Phytochemical profile was established using High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) and bioactive compound(s) docked against target enzymes using in silica methods. Cytotoxicity against three cancer cell lines was assessed using the methylthiazolyldiphenyl-tetrazolium bromide (MTT) cell viability test. The main compounds were luteolin-7-glucoside (cynaroside), chlorogenic acid, and epicatechin in the extracts. The methanol extract exhibited the highest radical scavenging activity. Ethyl acetate extract showed strongest a-amylase inhibitory activity, while the best a-glucosidase inhibitory effect recorded for the methanol extract. Molecular docking showed that cynaroside strongly interact to a-glucosidase cavity by establishing six hydrogen bonds. B. tripartita extracts were found to protect supercoiled form of pUC19 plasmid (>70%) and also showed anti-proliferative properties. Results amassed in the present study add on to a growing body of literature on the multi-pharmacological potency of B. tripartita which can be applied to bio-products development geared towards management of common diseases