根圏低温ストレス下のホウレンソウの栄養成分に関する分析

京都大学新制・論文博士博士(農学)乙第13423号論農博第2898号新制||農||1086(附属図書館)学位論文||R3||N5346(農学部図書室)(主査)教授 中嶋 洋, 教授 近藤 直, 教授 飯田 訓久学位規則第4条第2項該当Doctor of Agricultural ScienceKyoto UniversityDFA

Buckling Strength of Cold-Formed Circular Steel Column Subjected to Axial Load

In this study, the global buckling behavior of a cold-formed circular steel column was discussed with a focus on the effects of its mechanical properties and initial imperfections on the behavior. As the first step, the stress–strain curves of the column under tensile and compressive loads as well as its residual stresses were investigated. Subsequently, a finite element analysis was conducted to clarify if the analysis properly simulated the stub column behavior. The analysis results obtained using measured compressive stress–strain curves and residual stresses agreed well with experimental results. Finally, another finite element analysis was performed on the long column buckling to examine the effects of its mechanical properties and initial imperfections. It was shown that the global buckling strength was affected not only by imperfections such as residual stress and out-of-straightness but also by the anisotropic mechanical properties of the material

学内で創る模擬患者とのコミュニケーション実習～学内で学びを深める試み～

　本稿の目的は、新型コロナウイルス感染症（COVID-19）の影響を受け、臨地実習受け入れ中止に伴い学内で実施された「基礎看護学実習Ⅰ」における授業実践において、発展した学びを学内で提供する方略について検討することである。特に本稿では、臨地で実践を予定していたʻ患者との初めての出会いʼを効果的に学内で実施するための工夫について報告する。はじめに、基礎看護学実習Ⅰの目標・構成・実際のスケジュールについて概観し、続いて、模擬患者とのコミュニケーションの実際、学習を深めるための工夫について報告した。実践した学内での「基礎看護学実習Ⅰ」のコミュニケーションでは、第１にコミュニケーションの講義とロールプレイとその振り返りにより自己のコミュニケーションの特徴を知り、第2に自己分析と看護の意味の考察から看護師―患者関係を考察し、第３に模擬患者とのコミュニケーションを経験することにより、コミュニケーションの相互作用と深まりを実感することが示唆された。今後、看護学実習という臨地での学びに重きをおく授業形態の中で、学内で何を学ぶことができるのか考えていくことが課題である

Optogenetic Manipulation of Cerebellar Purkinje Cell Activity In Vivo

Purkinje cells (PCs) are the sole output neurons of the cerebellar cortex. Although their anatomical connections and physiological response properties have been extensively studied, the causal role of their activity in behavioral, cognitive and autonomic functions is still unclear because PC activity cannot be selectively controlled. Here we developed a novel technique using optogenetics for selective and rapidly reversible manipulation of PC activity in vivo. We injected into rat cerebellar cortex lentiviruses expressing either the light-activated cationic channel channelrhodopsin-2 (ChR2) or light-driven chloride pump halorhodopsin (eNpHR) under the control of the PC-specific L7 promoter. Transgene expression was observed in most PCs (ChR2, 92.6%; eNpHR, 95.3%), as determined by immunohistochemical analysis. In vivo electrophysiological recordings showed that all light-responsive PCs in ChR2-transduced rats increased frequency of simple spike in response to blue laser illumination. Similarly, most light-responsive PCs (93.8%) in eNpHR-transduced rats decreased frequency of simple spike in response to orange laser illumination. We then applied these techniques to characterize the roles of rat cerebellar uvula, one of the cardiovascular regulatory regions in the cerebellum, in resting blood pressure (BP) regulation in anesthetized rats. ChR2-mediated photostimulation and eNpHR-mediated photoinhibition of the uvula had opposite effects on resting BP, inducing depressor and pressor responses, respectively. In contrast, manipulation of PC activity within the neighboring lobule VIII had no effect on BP. Blue and orange laser illumination onto PBS-injected lobule IX didn't affect BP, indicating the observed effects on BP were actually due to PC activation and inhibition. These results clearly demonstrate that the optogenetic method we developed here will provide a powerful way to elucidate a causal relationship between local PC activity and functions of the cerebellum

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements