Pengaruh Aras Energi Pakan dan Skor Kondisi Tubuh terhadap Produksi dan Kualitas Fisik Daging Ternak Sapi Bali Betina Afkir

Penelitian dilakukan untuk mengetahui pengaruh aras energi dan kondisi tubuh terhadap produksi dan mutu daging sapi Bali betina afkir. Kajian ini menggunakan 18 ekor sapi Bali betina afkir yang kurus dengan skor kondisi tubuh 2. Penelitian menggunakan rancangan acak lengkap dengan aras energi sebagai faktor utama dan skor kondisi tubuh sebagai faktor tersarang. Pakan komplit fermentasi (2% dari berat badan) dengan level energi yang berbeda yaitu 7, 8 dan 9 MJ diberikan sebagai perlakuan. Penelitian berlangsung selama 3 bulan yang terdiri dari 2 minggu masa persiapan dan penyesuaian (preleminary) dan 10 minggu pengambilan (koleksi) data. Parameter utama adalah produksi karkas dan kualitas fisik serta kimia daging. Hasil penelitian menunjukkan bahwa tidak terdapat pengaruh (P>0.05) dari level energi dan skor kondisi tubuh pada parameter yang diukur. Sementara itu, nilai susut masak dan keempukan secara signifikan dipengaruhi oleh level energi pakan. Kesimpulannya bahwa penggemukan sapi Bali betina afkir dapat dilakukan secara efisien dalam periode singkat. Ada indikasi Perubahan skor kondisi tubuh yang memadai dengan pemberian pakan pada level energi metabolis 9 MJ

Effect of carrier and temperature on the viability of Burkholderia sp (UPMB3) and Pseudomonas sp (UPM P3) during storage.

This study was aimed at to determine the ability of different carriers to sustain the viability and efficacy of endophytic bacteria: Burkholderia sp (UPMB3) and Pseudomonas sp(UPMP3) during storage. UPMB3 and UPMP3 were formulated as dry formulation using vermiculite and coir dust as carriers and liquid formulation with Luria broth (LB) as the culture substrate. These bacterial formulations developed were assessed for the viability and efficacy as fresh preparations and after nine months stored at 10, 20 and 30°C. Formulations stored at 10 and 20°C provided a longer shelf-life than those stored at 30°C based on viability at monthly intervals over a 9-month storage period. At 10 and 20°C, the LB-based and vermiculite-based formulations were found to be the most stable by sustaining 86% of viable bacteria cells after 6 months of storage. However, at the end of 9 months, the number of viable bacteria cells in both formulations declined to 71 and 57%, respectively. Coir dust-based formulation was the least stable at 10 and 20°C storage, when only 43 and 29% viable cells were detected at the end of 9-months storage

The duration of embryo culture after mouse IVF differentially affects cardiovascular and metabolic health in male offspring

Study question: Do the long-term health outcomes following IVF differ depending upon the duration of embryo culture before transfer?Summary answer: Using a mouse model, we demonstrate that in male but not female offspring, adverse cardiovascular (CV) health was more likely with prolonged culture to the blastocyst stage, but metabolic dysfunction was more likely if embryo transfer (ET) occurred at the early cleavage stage.What is known already: ART associate with increased risk of adverse CV and metabolic health in offspring, and these findings have been confirmed in animal models in the absence of parental infertility issues. It is unclear which specific ART treatments may cause these risks. There is increasing use of blastocyst, versus cleavage-stage, transfer in clinical ART which does not appear to impair perinatal health of children born, but the longer-term health implications are unknown.Study design, size, duration: Five mouse groups were generated comprising: (i) natural mating (NM)-naturally mated, non-superovulated and undisturbed gestation; (ii) IV-ET-2Cell-in-vivo derived two-cell embryos collected from superovulated mothers, with immediate ET to recipients; (iii) IVF-ET-2Cell-IVF generated embryos, from oocytes from superovulated mothers, cultured to the two-cell stage before ET to recipients; (iv) IV-ET-BL-in-vivo derived blastocysts collected from superovulated mothers, with immediate ET to recipients; (v) IVF-ET-BL-IVF generated embryos, from oocytes from superovulated mothers, cultured to the blastocyst stage before ET to recipients. Both male and female offspring were analysed for growth, CV and metabolic markers of health. There were 8-13 litters generated for each group for analyses; postnatal data were analysed by multilevel random effects regression to take account of between-mother and within-mother variation and litter size.Participants/materials, settings, methods: C57/BL6 female mice (3-4 weeks old) were used for oocyte production; CBA males for sperm with human tubal fluid medium were used for IVF. Embryos were transferred (ET) to MF1 pseudo-pregnant recipients at the two-cell stage or cultured in synthetic oviductal medium enriched with potassium medium to the blastocyst stage before ET. Control in-vivo embryos from C57BL6 × CBA matings were collected and immediately transferred at the two-cell or blastocyst stage. Postnatal assays included growth rate up to 27 weeks; systolic blood pressure (SBP) at 9, 15 and 21 weeks; lung and serum angiotensin-converting enzyme (ACE) activity at time of cull (27 weeks); glucose tolerance test (GTT; 27 weeks); basal glucose and insulin levels (27 weeks); and lipid accumulation in liver cryosections using Oil Red O imaging (27 weeks).Main results and the role of chance: Blastocysts formed by IVF developed at a slower rate and comprised fewer cells that in-vivo generated blastocysts without culture (P < 0.05). Postnatal growth rate was increased in all four experimental treatments compared with NM group (P < 0.05). SBP, serum and lung ACE and heart/body weight were higher in IVF-ET-BL versus IVF-ET-2Cell males (P < 0.05) and higher than in other treatment groups, with SBP and lung ACE positively correlated (P < 0.05). Glucose handling (GTT AUC) was poorer and basal insulin levels were higher in IVF-ET-2Cell males than in IVF-ET-BL (P < 0.05) with the glucose:insulin ratio more negatively correlated with body weight in IVF-ET-2Cell males than in other groups. Liver/body weight and liver lipid droplet diameter and density in IVF-ET-2Cell males were higher than in IVF-ET-BL males (P < 0.05). IVF groups had poorer health characteristics than their in-vivo control groups, indicating that outcomes were not caused specifically by background techniques (superovulation, ET). No consistent health effects from duration of culture were identified in female offspring.Large scale data: N/A.Limitations, reasons for caution: Results from experimental animal models cannot be extrapolated to humans. Nevertheless, they are valuable to develop conceptual models, in this case, in the absence of confounding parental infertility, in assessing the safety of ART manipulations.Wider implications of the findings: The study indicates that longer duration of embryo culture after IVF up to blastocyst before ET leads to increased dysfunction of CV health in males compared with IVF and shorter cleavage-stage ET. However, the metabolic health of male offspring was poorer after shorter versus longer culture duration. This distinction indicates that the origin of CV and metabolic health phenotypes after ART may be different. The poorer metabolic health of males after cleavage-stage ET coincides with embryonic genome activation occurring at the time of ET

Focal Adhesion Remodeling Is Crucial for Glucose-Stimulated Insulin Secretion and Involves Activation of Focal Adhesion Kinase and Paxillin

Actin cytoskeleton remodeling is known to be involved in glucose-stimulated insulin secretion (GSIS). We have observed glucose-stimulated changes at the β-cell basal membrane similar to focal adhesion remodeling in cell migration. This led us to study the role of two key focal adhesion proteins, focal adhesion kinase (FAK) and paxillin, in GSIS

Planck 2013 results. I. Overview of products and scientific results

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