Political patronage in New Zealand : an exploratory study

This thesis is concerned with the theory and practice of political appointments in New Zealand. Little has been written on the subject of political patronage recently, and that literature which does exist is mainly concerned with the application of a moral theory to bureaucratic appointments. This thesis is an attempt to analyse areas of political patronage that lie outside the scope of previous works, and to apply and where relevant to judge the value of existing theories in this analysis. The first chapter is concerned with political patronage per se and areas of government in which it is used. Three indicators of moral acceptability are introduced (efficiency, equality of opportunity, and neutrality), and their value as theoretical tools is assessed. The second and third chapters are respectively concerned with case studies of the Royal Honours system, and the appointment of Justices of the Peace. As areas of political patronage these are studied in the light of the moral trichotomy mentioned above. The final conclusion is that political patronage is an acceptable method of appointment as long as the patron recognises and applies certain moral criteria in his selection of clients. With respect to the appointment of Justices of the Peace in particular, it seems that this blend of theory and practice is often lacking

Fortissat minewater geothermal district heating project: case study

This study summarises the findings of a feasibility assessment for a potential minewater geothermal energy system in the vicinity of the James Hutton Institute’s Hartwood Home Farm, North Lanarkshire in Scotland’s Central Belt. This study aimed to assess the potential for Scotland’s first minewater geothermal scheme in a rural area with social deprivation. While focused on the specifics of the location, the project is conceived as a readily replicable and fully operational minewater geothermal district heating system demonstrator project that would act as proof of concept for duplication elsewhere. This study concluded that, with current support mechanisms, the scheme is technically and financially viable, breaking even or better with a network scenario covering the ‘representative’ communities of Allanton and Hartwood. As might be expected, there are considerable economies of scale, with the scheme becoming commercially viable when the network is extended to the town of Shotts

Lung responses to secondary endotoxin challenge in rats exposed to pig barn air

<p>Abstract</p> <p>Background</p> <p>Swine barn air contains endotoxin and many other noxious agents. Single or multiple exposures to pig barn air induces lung inflammation and loss of lung function. However, we do not know the effect of exposure to pig barn air on inflammatory response in the lungs following a secondary infection. Therefore, we tested a hypothesis that single or multiple exposures to barn air will result in exaggerated lung inflammation in response to a secondary insult with <it>Escherichia coli </it>LPS (<it>E. coli </it>LPS).</p> <p>Methods</p> <p>We exposed Sprague-Dawley rats to ambient (N = 12) or swine barn air (N = 24) for one or five days and then half (N = 6/group) of these rats received intravenous <it>E. coli </it>LPS challenge, observed for six hours and then euthanized to collect lung tissues for histology, immunohistochemistry and ELISA to assess lung inflammation.</p> <p>Results</p> <p>Compared to controls, histological signs of lung inflammation were evident in barn exposed rat lungs. Rats exposed to barn air for one or five days and challenged with <it>E. coli </it>LPS showed increased recruitment of granulocytes compared to those exposed only to the barn. Control, one and five day barn exposed rats that were challenged with <it>E. coli </it>LPS showed higher levels of IL-1β in the lungs compared to respective groups not challenged with <it>E. coli </it>LPS. The levels of TNF-α in the lungs did not differ among any of the groups. Control rats without <it>E. coli </it>LPS challenge showed higher levels of TGF-β2 compared to controls challenged with <it>E. coli </it>LPS.</p> <p>Conclusion</p> <p>These results show that lungs of rats exposed to pig barn air retain the ability to respond to <it>E. coli </it>LPS challenge.</p

Multiple exposures to swine barn air induce lung inflammation and airway hyper-responsiveness

BACKGROUND: Swine farmers repeatedly exposed to the barn air suffer from respiratory diseases. However the mechanisms of lung dysfunction following repeated exposures to the barn air are still largely unknown. Therefore, we tested a hypothesis in a rat model that multiple interrupted exposures to the barn air will cause chronic lung inflammation and decline in lung function. METHODS: Rats were exposed either to swine barn (8 hours/day for either one or five or 20 days) or ambient air. After the exposure periods, airway hyper-responsiveness (AHR) to methacholine (Mch) was measured and rats were euthanized to collect bronchoalveolar lavage fluid (BALF), blood and lung tissues. Barn air was sampled to determine endotoxin levels and microbial load. RESULTS: The air in the barn used in this study had a very high concentration of endotoxin (15361.75 ± 7712.16 EU/m(3)). Rats exposed to barn air for one and five days showed increase in AHR compared to the 20-day exposed and controls. Lungs from the exposed groups were inflamed as indicated by recruitment of neutrophils in all three exposed groups and eosinophils and an increase in numbers of airway epithelial goblet cells in 5- and 20-day exposure groups. Rats exposed to the barn air for one day or 20 days had more total leukocytes in the BALF and 20-day exposed rats had more airway epithelial goblet cells compared to the controls and those subjected to 1 and 5 exposures (P < 0.05). Bronchus-associated lymphoid tissue (BALT) in the lungs of rats exposed for 20 days contained germinal centers and mitotic cells suggesting activation. There were no differences in the airway smooth muscle cell volume or septal macrophage recruitment among the groups. CONCLUSION: We conclude that multiple exposures to endotoxin-containing swine barn air induce AHR, increase in mucus-containing airway epithelial cells and lung inflammation. The data also show that prolonged multiple exposures may also induce adaptation in AHR response in the exposed subjects

Genes coding for virulence determinants of Campylobacter jejuni in human clinical and cattle isolates from Alberta, Canada, and their potential role in colonization of poultry

Forty nine Campylobacter jejuni isolates from cattle feces collected from Alberta feedlots and 50 clinical C. jejuni isolates from people in Alberta were tested for the presence of 14 genes encoding putative virulence factors by PCR. These included genes implicated in adherence and colonization (flaC, cadF, docC, racR, jlpA, peb1, and dnaJ), invasion (virB11, ciaB, pldA, and iamA) and protection against harsh conditions (htrA, cbrA, and sodB). The genes examined were widely distributed in both the cattle fecal isolates and the human isolates. Of the isolates tested, 67% contained all of the genes except virB11. The cadF gene was found in 100% of the isolates tested. The presence or absence of virulence-associated genes was not associated with the ability of the organism to colonize birds. All of the C. jejuni isolates used to challenge birds were able to colonize the animals regardless of virulence gene profile. While some diversity in the profile of the occurrence of virulence-associated genes in C. jejuni exists, the distribution of these putative virulence-associated genes isolates from feedlotcattle feces and humans in Alberta was similar. In addition it was not possible to predict the ability of the selected isolates tocolonize young chicks based on the presence of these genes coding for virulence determinants. [Int Microbiol 2011; 14(1):25-32

Evaluation of the upper respiratory tract in the horse during treadmill exercise - A review Part ll: Measurement of upper airway flow mechanics

Literature pertaining to the evaluation of the upper respiratory tract of the horse during exercise was reviewed. Articles were found by searching two databases. Videoendoscopy of the upper airways during exercise is presented in part I of this review. Part II describes upper airway pressure and airflow measurements for objective assessement of the prescence of a respiratory limitation. Different measurement techniques and definitions of upper airway pressure as well as airflow measurement techniques are described. Upper airway pressures and flow indices increase linearly with increasing exercise. Airflow resistance as caused by laryngeal hemiplegia grade IV increases negative upper airway pressure and limits inspiratory flow. Dorsal displacement of the soft palate alters both inspiratory and expiratory pressures

A mutation in the mitochondrial fission gene Dnm1l leads to cardiomyopathy

Mutations in a number of genes have been linked to inherited dilated cardiomyopathy (DCM). However, such mutations account for only a small proportion of the clinical cases emphasising the need for alternative discovery approaches to uncovering novel pathogenic mutations in hitherto unidentified pathways. Accordingly, as part of a large-scale N-ethyl-N-nitrosourea mutagenesis screen, we identified a mouse mutant, Python, which develops DCM. We demonstrate that the Python phenotype is attributable to a dominant fully penetrant mutation in the dynamin-1-like (Dnm1l) gene, which has been shown to be critical for mitochondrial fission. The C452F mutation is in a highly conserved region of the M domain of Dnm1l that alters protein interactions in a yeast two-hybrid system, suggesting that the mutation might alter intramolecular interactions within the Dnm1l monomer. Heterozygous Python fibroblasts exhibit abnormal mitochondria and peroxisomes. Homozygosity for the mutation results in the death of embryos midway though gestation. Heterozygous Python hearts show reduced levels of mitochondria enzyme complexes and suffer from cardiac ATP depletion. The resulting energy deficiency may contribute to cardiomyopathy. This is the first demonstration that a defect in a gene involved in mitochondrial remodelling can result in cardiomyopathy, showing that the function of this gene is needed for the maintenance of normal cellular function in a relatively tissue-specific manner. This disease model attests to the importance of mitochondrial remodelling in the heart; similar defects might underlie human heart muscle disease

The Action of Bax and Bcl-2 on T Cell Selection

T cell development and selection in the thymus are shaped by the induction of apoptosis. However, a direct role in T cell development and selection for any of the molecules known to regulate apoptosis has remained controversial. We have studied the effect of bax and bcl-2 transgenes in recombination activation gene 1–deficient (RAG-1−/−) mice transgenic for the major histocompatibility complex class I–restricted F5 T cell receptor. Overexpression of a bax transgene in the thymus seriously impairs the production of mature T cells, whereas bcl-2 overexpression greatly promotes it. The effect of bax and bcl-2 overexpression on antigen-induced negative selection was studied using fetal thymic organ cultures. This analysis showed that Bcl-2 strongly inhibits negative selection, whereas Bax does not affect it. Our data directly show that Bcl-2 family members have specific roles in T cell selection and also lend support to the hypothesis that Bax and Bcl-2 can antagonize each other's action in a certain apoptosis pathway while in another they can be functionally nonreciprocal

Nutrition for the ageing brain: towards evidence for an optimal diet

As people age they become increasingly susceptible to chronic and extremely debilitating brain diseases. The precise cause of the neuronal degeneration underlying these disorders, and indeed normal brain ageing remains however elusive. Considering the limits of existing preventive methods, there is a desire to develop effective and safe strategies. Growing preclinical and clinical research in healthy individuals or at the early stage of cognitive decline has demonstrated the beneficial impact of nutrition on cognitive functions. The present review is the most recent in a series produced by the Nutrition and Mental Performance Task Force under the auspice of the International Life Sciences Institute Europe (ILSI Europe). The latest scientific advances specific to how dietary nutrients and non-nutrient may affect cognitive ageing are presented. Furthermore, several key points related to mechanisms contributing to brain ageing, pathological conditions affecting brain function, and brain biomarkers are also discussed. Overall, findings are inconsistent and fragmented and more research is warranted to determine the underlying mechanisms and to establish dose-response relationships for optimal brain maintenance in different population subgroups. Such approaches are likely to provide the necessary evidence to develop research portfolios that will inform about new dietary recommendations on how to prevent cognitive decline