Cerebral Infarction Associated with Cocaine Use

We report the case of a young man with an acute infarction of the left putamen and caudate nucleus, whose symptoms appeared six hours after intranasal use of approximately 0.5 g of cocaine hydrochloride. It seems probable that in this patient cocaine consumption played a role in the development of stroke

The intriguing HI gas in NGC 5253: an infall of a diffuse, low-metallicity HI cloud?

(Abridged) We present new, deep HI line and 20-cm radio continuum data of the very puzzling blue compact dwarf galaxy NGC 5253, obtained with the ATCA as part of the `Local Volume HI Survey' (LVHIS). Our low-resolution HI maps show the disturbed HI morphology that NGC 5253 possesses, including tails, plumes and detached HI clouds. The high-resolution map reveals an HI plume at the SE and an HI structure at the NW that surrounds an Ha shell. We confirm that the kinematics of the neutral gas are highly perturbed and do not follow a rotation pattern. We discuss the outflow and infall scenarios to explain such disturbed kinematics, analyze the environment in which it resides, and compare it properties with those observed in similar star-forming dwarf galaxies. The radio-continuum emission of NGC 5253 is resolved and associated with the intense star-forming region at the center of the galaxy. We complete the analysis using multiwavelength data extracted from the literature. We estimate the SFR using this multiwavelength approach. NGC 5253 does not satisfy the Schmidt-Kennicutt law of star-formation, has a very low HI mass-to-light ratio when comparing with its stellar mass, and seems to be slightly metal-deficient in comparison with starbursts of similar baryonic mass. Taking into account all available multiwavelength data, we conclude that NGC 5253 is probably experiencing the infall of a diffuse, low-metallicity HI cloud along the minor axis of the galaxy, which is comprising the ISM and triggering the powerful starburst. The tidally disturbed material observed at the east and north of the galaxy is a consequence of this interaction, which probably started more than 100 Myr ago. The origin of this HI cloud may be related with a strong interaction between NGC 5253 and the late-type spiral galaxy M 83 in the past.Comment: 19 pages, 12 figures, accepted for publication in MNRA

A deep XMM-Newton observation of the ultraluminous X-ray source HoII X-1 : the case against a 1000 solar mass black hole

We present results from a 112 ks long look by XMM-Newton at the ultraluminous X-ray source (ULX) Holmberg II X-1, long thought to be the one of best candidates for the missing class of intermediate mass black holes (IMBHs). Our data comprises the first high quality XMM-Newton/RGS spectrum of an ULX, and an XMM-Newton/EPIC spectrum with unprecedented signal-to-noise. A detailed timing analysis shows that any variability on time-scales of minutes to hours is very weak (< few per cent fractional rms), though larger amplitude variations on much shorter time-scales could be hidden by photon counting statistics. This result suggests that if Ho II X-1 harbours an IMBH, then we are observing this source in a highly unusual and atypical state when compared with the known variability behaviour of other accreting systems of large mass. Moreover unlike Galactic X-ray binaries, our spectral analysis indicates the possible presence of an optically-thick low temperature corona. Taken together our timing and spectral analysis suggests that the compact companion is most-likely a high luminosity analogue of black hole binary systems similar to GRS 1915+105, the Galactic microquasar, harbouring a compact object of mass no greater than 100 solar masses.Comment: 8 pages, 6 figures, accepted for publication in MNRA

Trace analysis of environmental matrices by large-volume injection and liquid chromatography-mass spectrometry

The time-honored convention of concentrating aqueous samples by solid-phase extraction (SPE) is being challenged by the increasingly widespread use of large-volume injection (LVI) liquid chromatography–mass spectrometry (LC–MS) for the determination of traces of polar organic contaminants in environmental samples. Although different LVI approaches have been proposed over the last 40 years, the simplest and most popular way of performing LVI is known as single-column LVI (SC-LVI), in which a large-volume of an aqueous sample is directly injected into an analytical column. For the purposes of this critical review, LVI is defined as an injected sample volume that is ≥10% of the void volume of the analytical column. Compared with other techniques, SC-LVI is easier to set up, because it requires only small hardware modifications to existing autosamplers and, thus, it will be the main focus of this review. Although not new, SC-LVI is gaining acceptance and the approach is emerging as a technique that will render SPE nearly obsolete for many environmental applications.In this review, we discuss: the history and development of various forms of LVI; the critical factors that must be considered when creating and optimizing SC-LVI methods; and typical applications that demonstrate the range of environmental matrices to which LVI is applicable, for example drinking water, groundwater, and surface water including seawater and wastewater. Furthermore, we indicate direction and areas that must be addressed to fully delineate the limits of SC-LVI

Role of Plant-Specific N-Terminal Domain of Maize CK2β1 Subunit in CK2β Functions and Holoenzyme Regulation

Protein kinase CK2 is a highly pleiotropic Ser/Thr kinase ubiquituous in eukaryotic organisms. CK2 is organized as a heterotetrameric enzyme composed of two types of subunits: the catalytic (CK2α) and the regulatory (CK2β). The CK2β subunits enhance the stability, activity and specificity of the holoenzyme, but they can also perform functions independently of the CK2 tetramer. CK2β regulatory subunits in plants differ from their animal or yeast counterparts, since they present an additional specific N-terminal extension of about 90 aminoacids that shares no homology with any previously characterized functional domain. Sequence analysis of the N-terminal domain of land plant CK2β subunit sequences reveals its arrangement through short, conserved motifs, some of them including CK2 autophosphorylation sites. By using maize CK2β1 and a deleted version (ΔNCK2β1) lacking the N-terminal domain, we have demonstrated that CK2β1 is autophosphorylated within the N-terminal domain. Moreover, the holoenzyme composed with CK2α1/ΔNCK2β1 is able to phosphorylate different substrates more efficiently than CK2α1/CK2β1 or CK2α alone. Transient overexpression of CK2β1 and ΔNCK2β1 fused to GFP in different plant systems show that the presence of N-terminal domain enhances aggregation in nuclear speckles and stabilizes the protein against proteasome degradation. Finally, bimolecular fluorescence complementation (BiFC) assays show the nuclear and cytoplasmic location of the plant CK2 holoenzyme, in contrast to the individual CK2α/β subunits mainly observed in the nucleus. All together, our results support the hypothesis that the plant-specific N-terminal domain of CK2β subunits is involved in the down-regulation of the CK2 holoenzyme activity and in the stabilization of CK2β1 protein. In summary, the whole amount of data shown in this work suggests that this domain was acquired by plants for regulatory purposes

Patient-derived xenograft (PDX) models in basic and translational breast cancer research

Patient-derived xenograft (PDX) models of a growing spectrum of cancers are rapidly supplanting long-established traditional cell lines as preferred models for conducting basic and translational preclinical research. In breast cancer, to complement the now curated collection of approximately 45 long-established human breast cancer cell lines, a newly formed consortium of academic laboratories, currently from Europe, Australia, and North America, herein summarizes data on over 500 stably transplantable PDX models representing all three clinical subtypes of breast cancer (ER+, HER2+, and "Triple-negative" (TNBC)). Many of these models are well-characterized with respect to genomic, transcriptomic, and proteomic features, metastatic behavior, and treatment response to a variety of standard-of-care and experimental therapeutics. These stably transplantable PDX lines are generally available for dissemination to laboratories conducting translational research, and contact information for each collection is provided. This review summarizes current experiences related to PDX generation across participating groups, efforts to develop data standards for annotation and dissemination of patient clinical information that does not compromise patient privacy, efforts to develop complementary data standards for annotation of PDX characteristics and biology, and progress toward "credentialing" of PDX models as surrogates to represent individual patients for use in preclinical and co-clinical translational research. In addition, this review highlights important unresolved questions, as well as current limitations, that have hampered more efficient generation of PDX lines and more rapid adoption of PDX use in translational breast cancer research

The global abundance of tree palms

Aim Palms are an iconic, diverse and often abundant component of tropical ecosystems that provide many ecosystem services. Being monocots, tree palms are evolutionarily, morphologically and physiologically distinct from other trees, and these differences have important consequences for ecosystem services (e.g., carbon sequestration and storage) and in terms of responses to climate change. We quantified global patterns of tree palm relative abundance to help improve understanding of tropical forests and reduce uncertainty about these ecosystems under climate change. Location Tropical and subtropical moist forests. Time period Current. Major taxa studied Palms (Arecaceae). Methods We assembled a pantropical dataset of 2,548 forest plots (covering 1,191 ha) and quantified tree palm (i.e., ≥10 cm diameter at breast height) abundance relative to co‐occurring non‐palm trees. We compared the relative abundance of tree palms across biogeographical realms and tested for associations with palaeoclimate stability, current climate, edaphic conditions and metrics of forest structure. Results On average, the relative abundance of tree palms was more than five times larger between Neotropical locations and other biogeographical realms. Tree palms were absent in most locations outside the Neotropics but present in >80% of Neotropical locations. The relative abundance of tree palms was more strongly associated with local conditions (e.g., higher mean annual precipitation, lower soil fertility, shallower water table and lower plot mean wood density) than metrics of long‐term climate stability. Life‐form diversity also influenced the patterns; palm assemblages outside the Neotropics comprise many non‐tree (e.g., climbing) palms. Finally, we show that tree palms can influence estimates of above‐ground biomass, but the magnitude and direction of the effect require additional work. Conclusions Tree palms are not only quintessentially tropical, but they are also overwhelmingly Neotropical. Future work to understand the contributions of tree palms to biomass estimates and carbon cycling will be particularly crucial in Neotropical forests