Comparative study of real-time pcr (Taqman probe and sybr green), serological techniques (elisa, ifa and dat) and clinical signs evaluation, for the diagnosis of canine leishmaniasis in experimentally infected dogs

Canine leishmaniasis (CanL) diagnosis is not fully resolved. Currently, two specific methodologies are in continuous development, the detection of the parasite DNA or RNA in target organs and the detection of specific antibodies against Leishmania sp. For a correct diagnosis, it has been shown that the joint use of this type of test is necessary. In this work, a Sybr Green and a TaqMan Probe based on real time PCRs (qPCR) was performed for the detection of Leishmania sp. in order to correlate the results with clinicopathological and serological evaluations (IFA, ELISA and DAT) to propose an optimal biological sample to be used to detect the parasite in both early and late stages of the infection. A total of four samples were processed: conjunctival swabs, popliteal lymph node aspirates, bone marrow aspirates, and peripheral blood from experimentally infected dogs belonging to a larger study. Our results indicated that a single non-invasive sample (conjunctival swab) and the application of both types of qPCR would be reliable for determining Leishmania infection as well as the disease stage in dogs, thus avoiding bone marrow, lymph node aspirate or blood samples collection. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)

The restoration of San Pedro cliff at La Alhambra: calculation aspects

La muralla-palacio de La Alhambra, que es Patrimonio de la Humanidad, se ve amenazada por el avance del Tajo de San Pedro (Figura1), un diedro de 65,5 m de altura, que corta la colina de La Alhambra y que se ha situado a sólo 23,8 m de ella. Existe en la zona un régimen tectónico de extensión que afloja el terreno y activa la caída de lajas. Otra causa importante del deterioro del Tajo es la erosión. El coeficiente de seguridad al deslizamiento del Tajo sometido al terremoto de periodo de retorno de 1.000 años es de 0,73. La solución propuesta para su preservación es una malla de alambre postesada de alto límite elástico y revegetación autóctona. La presión que puede llegar a aplicar la malla en servicio eleva el coeficiente de seguridad con sismo a un valor próximo a uno. Mediante una simulación en ordenador se ha analizado el impacto ambiental.San Pedro cliff (Figure 1) is a dihedral 65.5 m high, which has progressed to place itself at 23.8 m from the Alhambra wall-palace that is a heritage of the Humankind. Active normal faults surrounding the cliff have created an extension tectonic regime that loosens the ground and activates the slab falls. One of the faces of the dihedral is a major fault. The cliff’s factor of safety subject to the 1000 years return period earthquake is 0.73. The preservation solution proposed is a post-tensioned high elastic limit wire mesh and autochthonous vegetation. With this reinforcement, the factor of safety under that earthquake raises to a value around one. A computer simulation has permitted to analyse the environmental impact.Este trabajo se ha realizado con ayuda del proyecto de investigación BIA 2004-01302 del Ministerio de Educación y Ciencia

New Solution of D=11 Supergravity on S^7 from D=4

A new static partially twisted solution of N=4, SO(4) gauged supergravity in D=11 is obtained in this work using Cveti\^c et al embedding of four dimensional into eleven dimensional supergravities. In four dimensions we get two solutions: an asymptotic one corresponding to $AdS_4$ and a near horizon fixed point solution of the form $AdS_2\times H_2$. Hence, while the former solution has 32 supercharges the latter turns out to have only 4 conserved. Moreover, we managed to find an exact interpolating solution, thus connecting the above two. Aiming at a future study of $AdS/CFT$ duality for the theory at hand we derived the Penrose limit of the four dimensional solutions. Interestingly the pp-wave limit of the near horizon solution suggests itself as being of the supernumerary supersymmetric type. In D=11 we exhibit the uplift of the four dimensional solutions: one associated to $AdS_4\times S^7$ and the other to a foliation of $AdS_2\times H_2 \times S^7$, as well as their pp-wave limits.Comment: 14 pages, LaTe

Diálogos sobre transdisciplina: los investigadores y su objeto de estudio

A la transdisciplinariedad se le ha definido como “una feliz transgresión de las fronteras entre las disciplinas” y es en este tono en que se presenta esta obra, que recopila las experiencias y reflexiones, las discusiones y propuestas de una veintena de investigadores y académicos que hablan sobre o desde la transdisciplina acerca de los temas de su interés o especialidad. La aproximación se da desde perspectivas académicas diversas y se adereza con expresiones estéticas que van desde la poesía hasta la pintura, a través de las cuales se busca ofrecer un espacio a las rutas posibles y limitaciones connaturales de acceder a la realidad para construir conocimiento “de frontera”, “en las fronteras”. Los abordajes son fruto de la exploración, filiación, encantos y desencantos por parte de los autores con la entidad de su búsqueda, quienes buscan contestar, entre otras, las siguientes cuestiones: ¿Cómo establecer un acercamiento transdisciplinar al objeto de estudio? ¿Qué hace a un objeto de estudio transdisciplinar? ¿Cómo impacta la transdisciplinariedad la identidad del académico? Una obra concebida desde una perspectiva más pedagógica que desde la doxa académica, con el interés de aportar una lectura amena para las reflexiones en torno a la trasgresión de las fronteras disciplinarias.ITESO, A.C

Biomic Specialization and Speciation Rates in Ruminants (Cetartiodactyla, Mammalia): A Test of the Resource-Use Hypothesis at the Global Scale

The resource-use hypothesis proposed by E.S. Vrba predicts that specialist species have higher speciation and extinction rates than generalists because they are more susceptible to environmental changes and vicariance. In this work, we test some of the predictions derived from this hypothesis on the 197 extant and recently extinct species of Ruminantia (Cetartiodactyla, Mammalia) using the biomic specialization index (BSI) of each species, which is based on its distribution within different biomes. We ran 10000 Monte Carlo simulations of our data in order to get a null distribution of BSI values against which to contrast the observed data. Additionally, we drew on a supertree of the ruminants and a phylogenetic likelihood-based method (QuaSSE) for testing whether the degree of biomic specialization affects speciation rates in ruminant lineages. Our results are consistent with the predictions of the resource-use hypothesis, which foretells a higher speciation rate of lineages restricted to a single biome (BSI = 1) and higher frequency of specialist species in biomes that underwent high degree of contraction and fragmentation during climatic cycles. Bovids and deer present differential specialization across biomes; cervids show higher specialization in biomes with a marked hydric seasonality (tropical deciduous woodlands and schlerophyllous woodlands), while bovids present higher specialization in a greater variety of biomes. This might be the result of divergent physiological constraints as well as a different biogeographic and evolutionary history

Single hadron response measurement and calorimeter jet energy scale uncertainty with the ATLAS detector at the LHC

The uncertainty on the calorimeter energy response to jets of particles is derived for the ATLAS experiment at the Large Hadron Collider (LHC). First, the calorimeter response to single isolated charged hadrons is measured and compared to the Monte Carlo simulation using proton-proton collisions at centre-of-mass energies of sqrt(s) = 900 GeV and 7 TeV collected during 2009 and 2010. Then, using the decay of K_s and Lambda particles, the calorimeter response to specific types of particles (positively and negatively charged pions, protons, and anti-protons) is measured and compared to the Monte Carlo predictions. Finally, the jet energy scale uncertainty is determined by propagating the response uncertainty for single charged and neutral particles to jets. The response uncertainty is 2-5% for central isolated hadrons and 1-3% for the final calorimeter jet energy scale.Comment: 24 pages plus author list (36 pages total), 23 figures, 1 table, submitted to European Physical Journal

Measurement of the cross-section and charge asymmetry of WW bosons produced in proton-proton collisions at s=8\sqrt{s}=8 TeV with the ATLAS detector

This paper presents measurements of the $W^+ \rightarrow \mu^+\nu$ and $W^- \rightarrow \mu^-\nu$ cross-sections and the associated charge asymmetry as a function of the absolute pseudorapidity of the decay muon. The data were collected in proton--proton collisions at a centre-of-mass energy of 8 TeV with the ATLAS experiment at the LHC and correspond to a total integrated luminosity of 20.2~\mbox{fb^{-1}}. The precision of the cross-section measurements varies between 0.8% to 1.5% as a function of the pseudorapidity, excluding the 1.9% uncertainty on the integrated luminosity. The charge asymmetry is measured with an uncertainty between 0.002 and 0.003. The results are compared with predictions based on next-to-next-to-leading-order calculations with various parton distribution functions and have the sensitivity to discriminate between them.Comment: 38 pages in total, author list starting page 22, 5 figures, 4 tables, submitted to EPJC. All figures including auxiliary figures are available at https://atlas.web.cern.ch/Atlas/GROUPS/PHYSICS/PAPERS/STDM-2017-13