13 research outputs found
Integration of genetic and physical maps of the Primula vulgaris S locus and localization by chromosome in situ hybridization
âąHeteromorphic flower development in Primula is controlled by the S locus. The S locus genes, which control anther position, pistil length and pollen size in pin and thrum flowers, have not yet been characterized. We have integrated S-linked genes, marker sequences and mutant phenotypes to create a map of the P. vulgaris S locus region that will facilitate the identification of key S locus genes. We have generated, sequenced and annotated BAC sequences spanning the S locus, and identified its chromosomal location. âąWe have employed a combination of classical genetics and three-point crosses with molecular genetic analysis of recombinants to generate the map. We have characterized this region by Illumina sequencing and bioinformatic analysis, together with chromosome in situ hybridization. âąWe present an integrated genetic and physical map across the P. vulgaris S locus flanked by phenotypic and DNA sequence markers. BAC contigs encompass a 1.5-Mb genomic region with 1 Mb of sequence containing 82 S-linked genes anchored to overlapping BACs. The S locus is located close to the centromere of the largest metacentric chromosome pair. âąThese data will facilitate the identification of the genes that orchestrate heterostyly in Primula and enable evolutionary analyses of the S locus
Rethinking responsibility and care for a postcolonial world
Both responsibility and care have much to offer in thinking through the relationalities that make up a postcolonial world. Although contemporary political systems often posit responsibility and care within the context of individuated and autonomous selves, geographers have done much to relocate responsibility and care within narratives of interdependency â spatially and temporally. They have argued that both terms offer a route for thinking about ethical geographical relations between myriad places. In this article we take this project further, by looking at how the nature and shape of these relationships might be construed in a postcolonial world. We suggest that, through a more critical engagement with postcolonial thinking, any exploration of existing practices of responsibility and care will not only reveal the enormous potential of imagining these geographies as forms of existing and evolving relationalities, but will also lead us to interrogate the deployments of these terms in the context of past and present inequalities. We show that routing care and responsibility through postcolonial geographies moves us towards a more pragmatic responsiveness, one that involves a âcare-fullâ recognition of postcolonial interaction