Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production

Background: Many microorganisms possess enzymes that can efficiently degrade lignocellulosic materials, but donot have the capability to produce a large amount of ethanol. Thus, attempts have been made to transform suchenzymes into fermentative microbes to serve as hosts for ethanol production. However, an efficient host for aconsolidated bioprocess (CBP) remains to be found. For this purpose, a synthetic biology technique that cantransform multiple genes into a genome is instrumental. Moreover, a strategy to select cellulases that interactsynergistically is needed.Results: To engineer a yeast for CBP bio-ethanol production, a synthetic biology technique, called “promoter-basedgene assembly and simultaneous overexpression” (PGASO), that can simultaneously transform and express multiplegenes in a kefir yeast, Kluyveromyces marxianus KY3, was recently developed. To formulate an efficient cellulasecocktail, a filter-paper-activity assay for selecting heterologous cellulolytic enzymes was established in this study andused to select five cellulase genes, including two cellobiohydrolases, two endo-β-1,4-glucanases and onebeta-glucosidase genes from different fungi. In addition, a fungal cellodextrin transporter gene was chosen totransport cellodextrin into the cytoplasm. These six genes plus a selection marker gene were one-step assembledinto the KY3 genome using PGASO. Our experimental data showed that the recombinant strain KR7 could expressthe five heterologous cellulase genes and that KR7 could convert crystalline cellulose into ethanol.Conclusion: Seven heterologous genes, including five cellulases, a cellodextrin transporter and a selection marker,were simultaneously transformed into the KY3 genome to derive a new strain, KR7, which could directly convertcellulose to ethanol. The present study demonstrates the potential of our strategy of combining a cocktailformulation protocol and a synthetic biology technique to develop a designer yeast host

Rationality-Robust Information Design: Bayesian Persuasion under Quantal Response

Classic mechanism/information design imposes the assumption that agents are fully rational, meaning each of them always selects the action that maximizes her expected utility. Yet many empirical evidence suggests that human decisions may deviate from this full rationality assumption. In this work, we attempt to relax the full rationality assumption with bounded rationality. Specifically, we formulate the bounded rationality of an agent by adopting the quantal response model (McKelvey and Palfrey, 1995). We develop a theory of rationality-robust information design in the canonical setting of Bayesian persuasion (Kamenica and Gentzkow, 2011) with binary receiver action. We first identify conditions under which the optimal signaling scheme structure for a fully rational receiver remains optimal or approximately optimal for a boundedly rational receiver. In practice, it might be costly for the designer to estimate the degree of the receiver's bounded rationality level. Motivated by this practical consideration, we then study the existence and construction of robust signaling schemes when there is uncertainty about the receiver's bounded rationality level

Competitive Information Disclosure with Multiple Receivers

This paper analyzes a model of competition in Bayesian persuasion in which two symmetric senders vie for the patronage of multiple receivers by disclosing information about the qualities (i.e., binary state -- high or low) of their respective proposals. Each sender is allowed to commit to a signaling policy where he sends a private (possibly correlated) signal to every receiver. The sender's utility is a monotone set function of receivers who make a patron to this sender. We characterize the equilibrium structure and show that the equilibrium is not unique (even for simple utility functions). We then focus on the price of stability (PoS) in the game of two senders -- the ratio between the best of senders' welfare (i.e., the sum of two senders' utilities) in one of its equilibria and that of an optimal outcome. When senders' utility function is anonymous submodular or anonymous supermodular, we analyze the relation between PoS with the ex ante qualities $\lambda$ (i.e., the probability of high quality) and submodularity or supermodularity of utility functions. In particular, in both families of utility function, we show that $\text{PoS} = 1$ when the ex ante quality $\lambda$ is weakly smaller than $1/2$, that is, there exists equilibrium that can achieve welfare in the optimal outcome. On the other side, we also prove that $\text{PoS} > 1$ when the ex ante quality $\lambda$ is larger than $1/2$, that is, there exists no equilibrium that can achieve the welfare in the optimal outcome. We also derive the upper bound of $\text{PoS}$ as a function of $\lambda$ and the properties of the value function. Our analysis indicates that the upper bound becomes worse as the ex ante quality $\lambda$ increases or the utility function becomes more supermodular (resp.\ submodular)

SDSS J013127.34−-032100.1: A newly discovered radio-loud quasar at z=5.18z=5.18 with extremely high luminosity

Only very few z>5 quasars discovered to date are radio-loud, with a radio-to-optical flux ratio (radio-loudness parameter) higher than 10. Here we report the discovery of an optically luminous radio-loud quasar, SDSS J013127.34-032100.1 (J0131-0321 in short), at z=5.18+-0.01 using the Lijiang 2.4m and Magellan telescopes. J0131-0321 has a spectral energy distribution consistent with that of radio-loud quasars. With an i-band magnitude of 18.47 and radio flux density of 33 mJy, its radio-loudness parameter is ~100. The optical and near-infrared spectra taken by Magellan enable us to estimate its bolometric luminosity to be L_bol ~ 1.1E48 erg/s, approximately 4.5 times greater than that of the most distant quasar known to date. The black hole mass of J0131-0321 is estimated to be 2.7E9 solar masses, with an uncertainty up to 0.4 dex. Detailed physical properties of this high-redshift, radio-loud, potentially super-Eddington quasar can be probed in the future with more dedicated and intensive follow-up observations using multi-wavelength facilities.Comment: 5 pages, 3 figures, accepted to ApJ

Measurement of the inclusive and dijet cross-sections of b-jets in pp collisions at sqrt(s) = 7 TeV with the ATLAS detector

The inclusive and dijet production cross-sections have been measured for jets containing b-hadrons (b-jets) in proton-proton collisions at a centre-of-mass energy of sqrt(s) = 7 TeV, using the ATLAS detector at the LHC. The measurements use data corresponding to an integrated luminosity of 34 pb^-1. The b-jets are identified using either a lifetime-based method, where secondary decay vertices of b-hadrons in jets are reconstructed using information from the tracking detectors, or a muon-based method where the presence of a muon is used to identify semileptonic decays of b-hadrons inside jets. The inclusive b-jet cross-section is measured as a function of transverse momentum in the range 20 < pT < 400 GeV and rapidity in the range |y| < 2.1. The bbbar-dijet cross-section is measured as a function of the dijet invariant mass in the range 110 < m_jj < 760 GeV, the azimuthal angle difference between the two jets and the angular variable chi in two dijet mass regions. The results are compared with next-to-leading-order QCD predictions. Good agreement is observed between the measured cross-sections and the predictions obtained using POWHEG + Pythia. MC@NLO + Herwig shows good agreement with the measured bbbar-dijet cross-section. However, it does not reproduce the measured inclusive cross-section well, particularly for central b-jets with large transverse momenta.Comment: 10 pages plus author list (21 pages total), 8 figures, 1 table, final version published in European Physical Journal

Observation of associated near-side and away-side long-range correlations in √sNN=5.02 TeV proton-lead collisions with the ATLAS detector

Two-particle correlations in relative azimuthal angle (Δϕ) and pseudorapidity (Δη) are measured in √sNN=5.02  TeV p+Pb collisions using the ATLAS detector at the LHC. The measurements are performed using approximately 1  μb-1 of data as a function of transverse momentum (pT) and the transverse energy (ΣETPb) summed over 3.1<η<4.9 in the direction of the Pb beam. The correlation function, constructed from charged particles, exhibits a long-range (2<|Δη|<5) “near-side” (Δϕ∼0) correlation that grows rapidly with increasing ΣETPb. A long-range “away-side” (Δϕ∼π) correlation, obtained by subtracting the expected contributions from recoiling dijets and other sources estimated using events with small ΣETPb, is found to match the near-side correlation in magnitude, shape (in Δη and Δϕ) and ΣETPb dependence. The resultant Δϕ correlation is approximately symmetric about π/2, and is consistent with a dominant cos⁡2Δϕ modulation for all ΣETPb ranges and particle pT

Dish Discovery via Word Embeddings on Restaurant Reviews

ABSTRACT This paper proposes a novel framework for automatic dish discovery via word embeddings on restaurant reviews. We collect a dataset of user reviews from Yelp and parse the reviews to extract dish words. Then, we utilize the processed reviews as training texts to learn the embedding vectors of words via the skip-gram model. In the paper, a nearestneighbor like score function is proposed to rank the dishes based on their learned representations. We brief some analyses on the preliminary experiments and present a web-based visualization at http://clip.csie.org/yelp/. Keywords dish discovery, word embeddings, dish-word extraction BACKGROUND With the growth of social media, corporations, such as Yelp, have accumulated a great number of user generated content (UGC). In the literature, some studies have been conducted with a perspective of finding critical information hidden in the content METHODOLOGY Copyright held by the author(s). RecSys 2016 Poster Proceedings, September 15-19, 2016, USA, Boston. Our methodology mainly consists of three parts: 1) dishword recognition, 2) word embedding learning, and 3) dish score calculation. As alluded to earlier, UGC usually incorporates a degree of noise and different language usages; therefore, extracting dish names from user reviews is a complicated task. For example, observed from the dataset, users tend not to write the full name of a dish in their reviews; instead, the last word or the last two words are often written in the reviews. To grapple with this issue, we use regular expressions (regexps) to extract dish names from the user reviews. However, this also give rise to an issue that a certain dish in a restaurant may be of the same name in other restaurants, which may induce the problem of ambiguity and lower the accuracy of matching the correct dish name. So, we attach a dish name with its restaurant name to solve the ambiguity problem. We then utilize the collection of processed reviews as training texts to learn embeddings of each word in the reviews via a continuous space language model, the skip-gram model. After the training phase, each word (including every dish) is represented by an n-dimensional vector (called the embedding of this word). Inspired by the k-nearest neighbors algorithm, we define the score for every dish d as: where , m is the total number of positive sentiment words considered, λi (i = 1, · · · , m) is a weighting parameter. In addition, si denotes the i-nearest positive sentiment words of the given dish d, and w d , ws i ∈ R n are the vector representations of the dish d and the sentiment word si, respectively. In an extreme case (1) of λm = 1 and λi = 0 for i = 1, · · · , m − 1, this score function implements the concept of the average Euclidean distance between a dish and all the positive sentiment words; while in the case (2) λ1 = 1 and λi = 0 for i = 2, · · · , m, the scored is obtained with the closest positive sentiment words to the dish. EXPERIMENTS Our preliminary experiments involve a real-world restaurant review dataset collected from Yelp Data Challenge

Search for R-parity-violating supersymmetry in events with four or more leptons in sqrt(s) =7 TeV pp collisions with the ATLAS detector

A search for new phenomena in final states with four or more leptons (electrons or muons) is presented. The analysis is based on 4.7 fb−1 of $\sqrt{s}=7\;\mathrm{TeV}$ proton-proton collisions delivered by the Large Hadron Collider and recorded with the ATLAS detector. Observations are consistent with Standard Model expectations in two signal regions: one that requires moderate values of missing transverse momentum and another that requires large effective mass. The results are interpreted in a simplified model of R-parity-violating supersymmetry in which a 95% CL exclusion region is set for charged wino masses up to 540 GeV. In an R-parity-violating MSUGRA/CMSSM model, values of m 1/2 up to 820 GeV are excluded for 10 < tan β < 40

A Biomimetic Membrane Device That Modulates the Excessive Inflammatory Response to Sepsis

OBJECTIVE: Septic shock has a clinical mortality rate approaching fifty percent. The major clinical manifestations of sepsis are due to the dysregulation of the host's response to infection rather than the direct consequences of the invading pathogen. Central to this initial immunologic response is the activation of leukocytes and microvascular endothelium resulting in cardiovascular instability, lung injury and renal dysfunction. Due to the primary role of leukocyte activation in the sepsis syndrome, a synthetic biomimetic membrane, called a selective cytopheretic device (SCD), was developed to bind activated leukocytes. The incorporation of the SCD along an extracorporeal blood circuit coupled with regional anticoagulation with citrate to lower blood ionized calcium was devised to modulate leukocyte activation in sepsis. DESIGN: Laboratory investigation. SETTING: University of Michigan Medical School. SUBJECTS: Pigs weighing 30-35 kg. INTERVENTIONS: To assess the effect of the SCD in septic shock, pigs were administered 30×10(10) bacteria/kg body weight of Escherichia coli into the peritoneal cavity and within 1 hr were immediately placed in an extracorporeal circuit containing SCD. MEASUREMENTS AND MAIN RESULTS: In this animal model, the SCD with citrate compared to control groups without the SCD or with heparin anticoagulation ameliorated the cardiovascular instability and lung sequestration of activated leukocytes, reduced renal dysfunction and improved survival time compared to various control groups. This effect was associated with minimal elevations of systemic circulating neutrophil activation. CONCLUSIONS: These preclinical studies along with two favorable exploratory clinical trials form the basis of an FDA-approved investigational device exemption for a pivotal multicenter, randomized control trial currently underway