Persistence, spatial distribution and implications for progression detection of blind parts of the visual field in glaucoma: A clinical cohort study

Background: Visual field testing is an essential part of glaucoma care. It is hampered by variability related to the disease itself, response errors and fatigue. In glaucoma, blind parts of the visual field contribute to the diagnosis but - once established - not to progression detection; they only increase testing time. The aims of this study were to describe the persistence and spatial distribution of blind test locations in standard automated perimetry in glaucoma and to explore how the omission of presumed blind test locations would affect progression detection. Methodology/Principal Findings: Data from 221 eyes of 221 patients from a cohort study with the Humphrey Field Analyzer with 30-2 grid were used. Patients were stratified according to baseline mean deviation (MD) in six strata of 5 dB width each. For one, two, three and four consecutive 0.1 for all strata). Omitting test locations with three consecutive <0 dB sensitivities at baseline did not affect the performance of the MD-based Nonparametric Progression Analysis progression detection algorithm. Conclusions/Significance: Test locations that have been shown to be reproducibly blind tend to display a reasonable blindness persistence and do no longer contribute to progression detection. There is no clinically useful universal MD cut-off value beyond which testing can be limited to 10 degree eccentricity

Cognitive performance of young and elderly subjects on the free word recall memory test: effect of presentation order on recall order

The influence of aging on memory has been extensively studied, but the importance of short-term memory and recall sequence has not. The objective of the current study was to examine the recall order of words presented on lists and to determine if age affects recall sequence. Physically and psychologically healthy male subjects were divided into two groups according to age, i.e., 23 young subjects (20 to 30 years) and 50 elderly subjects (60 to 70 years) submitted to the Wechsler Adult Intelligence Scale-Revised and the free word recall test. The order of word presentation significantly affected the 3rd and 4th words recalled (P < 0.01; F = 14.6). In addition, there was interaction between the presentation order and the type of list presented (P < 0.05; F = 9.7). Also, both groups recalled the last words presented from each list (words 13-15) significantly more times 3rd and 4th than words presented in all remaining positions (P < 0.01). The order of word presentation also significantly affected the 5th and 6th words recalled (P = 0.05; F = 7.5) and there was a significant interaction between the order of presentation and the type of list presented (P < 0.01; F = 20.8). The more developed the cognitive functions, resulting mainly from formal education, the greater the cognitive reserve, helping to minimize the effects of aging on the long-term memory (episodic declarative).Associação Fundo de Incentivo à Psicofarmacologia Instituto do SonoUniversidade Federal de São Paulo (UNIFESP) Departamento de PsicobiologiaUNIFESP, Depto. de PsicobiologiaSciEL

The Cardiology Audit and Registration Data Standards (CARDS), European data standards for clinical cardiology practice

AIMS: Systematic registration of data from clinical practice is important for clinical care, local, national and international registries, and audit. Data to be collected for these different purposes should be harmonized. Therefore, during Ireland's Presidency of the European Union (EU) (January to June 2004), the Department of Health and Children worked with the European Society of Cardiology, the Irish Cardiac Society, and the European Commission to develop data standards for clinical cardiology. The Cardiology Audit and Registration Data Standards (CARDS) Project aimed to agree standards for three modules of cardiovascular health information systems: acute coronary syndromes (ACS), percutaneous coronary interventions (PCI), and clinical electrophysiology (pacemakers, implantable cardioverter defibrillators, and ablation procedures). METHODS AND RESULTS: Data items from existing registries and surveys were reviewed to derive draft data standards (variables, coding, and definitions). Variables common to the three modules include demographics, risk factors, medication, and discharge and follow-up data. Modules about a procedure contain variables on the l

Calcium and mitochondrial metabolism in ceramide-induced cardiomyocyte death

AbstractCeramides are important intermediates in the biosynthesis and degradation of sphingolipids that regulate numerous cellular processes, including cell cycle progression, cell growth, differentiation and death. In cardiomyocytes, ceramides induce apoptosis by decreasing mitochondrial membrane potential and promoting cytochrome-c release. Ca2+ overload is a common feature of all types of cell death. The aim of this study was to determine the effect of ceramides on cytoplasmic Ca2+ levels, mitochondrial function and cardiomyocyte death. Our data show that C2-ceramide induces apoptosis and necrosis in cultured cardiomyocytes by a mechanism involving increased Ca2+ influx, mitochondrial network fragmentation and loss of the mitochondrial Ca2+ buffer capacity. These biochemical events increase cytosolic Ca2+ levels and trigger cardiomyocyte death via the activation of calpains

Association of EWS-FLI1 Type 1 Fusion with Lower Proliferative Rate in Ewing’s Sarcoma

The Ewing's sarcoma (ES) family of tumors, including peripheral neuroectodermal tumor (PNET), is defined genetically by specific chromosomal translocations resulting in fusion of the EWS gene with a member of the ETS family of transcription factors, either FLI1 (90-95%) or ERG (5-10%). A second level of molecular genetic heterogeneity stems from the variation in the location of the translocation breakpoints, resulting in the inclusion of different combinations of exons from EWS and FLI1 (or ERG) in the fusion products. The most common type of EWS-FLI1 fusion transcript, type 1, is associated with a favorable prognosis and appears to encode a functionally weaker transactivator, compared to other fusion types. We sought to determine whether the observed covariation of structure, function, and clinical course correlates with tumor cell kinetic parameters such as proliferative rate and apoptosis, and with expression of the receptor for insulin-like growth factor I (IGF-1R). In a group of 86 ES/PNET with defined EWS-ETS fusions (45 EWS-FLI1 type 1, 27 EWS-FLI1 non-type 1, 14 EWS-ERG), we assessed proliferation rate by immunostaining for Ki-67 using MIB1 antibody (n = 85), apoptosis by TUNEL assay (n = 66), and IGF-1R expression by immunostaining with antibody 1H7 (n = 78). Ki-67 proliferative index was lower in tumors with EWS-FLI1 type 1 than those with non-type 1 EWS-FLI1, whether analyzed as a continuous (P = 0.049) or categorical (P = 0.047) variable. Logistic regression analysis suggests that this association was secondary to the association of type 1 EWS-FLI1 and lower IGF-1R expression (P = 0.04). Comparing EWS-FLI1 to EWS-ERG cases, Ki-67 proliferative index was higher in the latter (P = 0.01, Mann-Whitney test; P = 0.02, Fisher's exact test), but there was no significant difference in IGF-1R. TUNEL results showed no significant differences between groups. Our results suggest that clinical and functional differences between alternative forms of EWS-FLI1 are paralleled by differences in proliferative rate, possibly mediated by differential regulation of the IGF-1R pathway

Estimación de caudales ecológicos basada en simulación del hábitat físico en un pequeño río del sudeste de Brasil

El método de simulación del hábitat físico, integrado en el marco de la metodología IFIM (Instream Flow Incremental Methodology) y en otros marcos metodológicos hoy día, utiliza variables hidráulicas e índices de idoneidad del hábitat (a escala de microhábitat) para predecir los cambios potenciales del hábitat al cambiar el caudal circulante. Este enfoque se ha utilizado para simular los cambios potenciales de idoneidad del hábitat para tres especies de peces tropicales de diferentes órdenes, Bryconamericus ornaticeps, Ancistrus multispinis y Geophagus brasiliensis. El estudio se realizó en dos tramos de un río en la zona de mata atlántica al sudeste de Brasil, durante el verano de 2013 e invierno de 2014. Se midieron las características topográficas e hidráulicas por transectos (calado, velocidad media, tipos de sustrato) y se estudió el uso del microhábitat por los peces mediante observación directa bajo el agua (snorkelling); esto permitió elaborar curvas de idoneidad de microhábitat para las tres especies. Mediante la simulación del hábitat físico se obtuvieron las curvas que relacionan dos indicadores, el HSI (indice medio de idoneidad en un tramo), y el HPU (Hábitat Potencial Útil) con el caudal del río. Sobre dichas curvas se observaron los caudales importantes para el mantenimiento de los hábitats de las tres especies. Los resultados indicaron un caudal mínimo-minimorum de 0.25 m3 s-1 en época seca para conservar el hábitat de los peces. También se determinó un rango óptimo de 0.65-0.85 m3 s-1 para el hábitat de las tres especies. Los resultados pueden apoyar la elaboración de una primera propuesta de régimen ecológico de caudales, en el marco de un proceso de gestión adaptativa de los recursos hídricos, para conservar la salud del río São Pedro y los diversos servicios ecosistémicos que aporta a la sociedad.Da Costa, M.; Moretti Mattos, T.; Muñoz Mas, R.; Martinez-Capel, F.; Fernandes, V.; Aráujo, F. (2015). Estimación de caudales ecológicos basada en simulación del hábitat físico en un pequeño río del sudeste de Brasil. Cuadernos de la Sociedad Española de Ciencias Forestales. (41):245-252. http://hdl.handle.net/10251/60209S2452524

Coherent spin valve phenomena and electrical spin injection in ferromagnetic/semiconductor/ferromagnetic junctions

Coherent quantum transport in ferromagnetic/ semiconductor/ ferromagnetic junctions is studied theoretically within the Landauer framework of ballistic transport. We show that quantum coherence can have unexpected implications for spin injection and that some intuitive spintronic concepts which are founded in semi-classical physics no longer apply: A quantum spin-valve (QSV) effect occurs even in the absence of a net spin polarized current flowing through the device, unlike in the classical regime. The converse effect also arises, i.e. a zero spin-valve signal for a non-vanishing spin-current. We introduce new criteria useful for analyzing quantum and classical spin transport phenomena and the relationships between them. The effects on QSV behavior of spin-dependent electron transmission at the interfaces, interface Schottky barriers, Rashba spin-orbit coupling and temperature, are systematically investigated. While the signature of the QSV is found to be sensitive to temperature, interestingly, that of its converse is not. We argue that the QSV phenomenon can have important implications for the interpretation of spin-injection in quantum spintronic experiments with spin-valve geometries.Comment: 15 pages including 11 figures. To appear in PR

Efeitos do clima, da poluição atmosférica e do estado sanitário das árvores na produção e nas características dos pólens alergisantes

Os pólens das árvores urbanas podem participar decisivamente na manifestação de doenças respiratórias alérgicas. Por outro lado, o estado sanitário das árvores e a poluição do ar podem influenciar a quantidade, características e época de produção de pólen. Neste contexto, iniciou-se um projecto de investigação visando: (a) desenvolver o conhecimento das relações entre produção de pólen e elementos do clima, poluição atmosférica e estado sanitário das árvores; (b) explorar metodologias expeditas e não destrutivas para a identificação de pólens, por espectrofotometria de infravermelho próximo e médio (NIR e FTIR); (c) desenvolver critérios técnicos para melhorar a selecção e condução de árvores em áreas urbanas sensíveis. A área principal de monitorização do projecto é o Campo Grande, em Lisboa, onde se monitoriza regularmente a qualidade do ar (estação de Entrecampos), se captura por amostragem pólen atmosférico e se avalia regularmente o estado sanitário das árvores envolventes. A recolha de amostras de pólen para análise através de NIR e FTIR será também efectuada noutras áreas de Lisboa, para a constituição de catálogos espectrais. O aprofundamento e a difusão de conhecimentos neste domínio tem um grande potencial para a melhoria da qualidade de vida da população urbana e peri-urbana sensível às alergias respiratórias

Measurement of the polarisation of W bosons produced with large transverse momentum in pp collisions at sqrt(s) = 7 TeV with the ATLAS experiment

This paper describes an analysis of the angular distribution of W->enu and W->munu decays, using data from pp collisions at sqrt(s) = 7 TeV recorded with the ATLAS detector at the LHC in 2010, corresponding to an integrated luminosity of about 35 pb^-1. Using the decay lepton transverse momentum and the missing transverse energy, the W decay angular distribution projected onto the transverse plane is obtained and analysed in terms of helicity fractions f0, fL and fR over two ranges of W transverse momentum (ptw): 35 < ptw < 50 GeV and ptw > 50 GeV. Good agreement is found with theoretical predictions. For ptw > 50 GeV, the values of f0 and fL-fR, averaged over charge and lepton flavour, are measured to be : f0 = 0.127 +/- 0.030 +/- 0.108 and fL-fR = 0.252 +/- 0.017 +/- 0.030, where the first uncertainties are statistical, and the second include all systematic effects.Comment: 19 pages plus author list (34 pages total), 9 figures, 11 tables, revised author list, matches European Journal of Physics C versio