A dense disk of dust around the born-again Sakurai's object

In 1996, Sakurai's object (V4334 Sgr) suddenly brightened in the centre of a faint Planetary Nebula (PN). This very rare event was interpreted as the reignition of a hot white dwarf that caused a rapid evolution back to the cool giant phase. From 1998 on, a copious amount of dust has formed continuously, screening out the star which has remained embedded in this expanding high optical depth envelope. The new observations, reported here, are used to study the morphology of the circumstellar dust in order to investigate the hypothesis that Sakurai's Object is surrounded by a thick spherical envelope of dust. We have obtained unprecedented, high-angular resolution spectro-interferometric observations, taken with the mid-IR interferometer MIDI/VLTI, which resolve the dust envelope of Sakurai's object. We report the discovery of a unexpectedly compact (30 x 40 milliarcsec, 105 x 140 AU assuming a distance of 3.5 kpc), highly inclined, dust disk. We used Monte Carlo radiative-transfer simulations of a stratified disk to constrain its geometric and physical parameters, although such a model is only a rough approximation of the rapidly evolving dust structure. Even though the fits are not fully satisfactory, some useful and robust constraints can be inferred. The disk inclination is estimated to be 75+/-3 degree with a large scale height of 47+/-7 AU. The dust mass of the disk is estimated to be 6 10^{-5} solar mass. The major axis of the disk (132+/-3 degree) is aligned with an asymmetry seen in the old PN that was re-investigated as part of this study. This implies that the mechanism responsible for shaping the dust envelope surrounding Sakurai's object was already at work when the old PN formed.Comment: A&A Letter, accepte

PepShell : visualization of conformational proteomics data

Proteins are dynamic molecules; they undergo crucial conformational changes induced by post-translational modifications and by binding of cofactors or other molecules. The characterization of these conformational changes and their relation to protein function is a central goal of structural biology. Unfortunately, most conventional methods to obtain structural information do not provide information on protein dynamics. Therefore, mass spectrometry-based approaches, such as limited proteolysis, hydrogen-deuterium exchange, and stable-isotope labeling, are frequently used to characterize protein conformation and dynamics, yet the interpretation of these data can be cumbersome and time consuming. Here, we present PepShell, a tool that allows interactive data analysis of mass spectrometry-based conformational proteomics studies by visualization of the identified peptides both at the sequence and structure levels. Moreover, PepShell allows the comparison of experiments under different conditions, including different proteolysis times or binding of the protein to different substrates or inhibitors

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

Current antibiotics tend to be broad spectrum, leading to indiscriminate killing of commensal bacteria and accelerated evolution of drug resistance. Here, we use CRISPR-Cas technology to create antimicrobials whose spectrum of activity is chosen by design. RNA-guided nucleases (RGNs) targeting specific DNA sequences are delivered efficiently to microbial populations using bacteriophage or bacteria carrying plasmids transmissible by conjugation. The DNA targets of RGNs can be undesirable genes or polymorphisms, including antibiotic resistance and virulence determinants in carbapenem-resistant Enterobacteriaceae and enterohemorrhagic Escherichia coli. Delivery of RGNs significantly improves survival in a Galleria mellonella infection model. We also show that RGNs enable modulation of complex bacterial populations by selective knockdown of targeted strains based on genetic signatures. RGNs constitute a class of highly discriminatory, customizable antimicrobials that enact selective pressure at the DNA level to reduce the prevalence of undesired genes, minimize off-target effects and enable programmable remodeling of microbiota.National Institutes of Health (U.S.) (New Innovator Award 1DP2OD008435)National Centers for Systems Biology (U.S.) (Grant 1P50GM098792)United States. Defense Threat Reduction Agency (HDTRA1-14-1-0007)Massachusetts Institute of Technology. Institute for Soldier Nanotechnologies (W911NF13D0001)National Institute of General Medical Sciences (U.S.) (Interdepartmental Biotechnology Training Program 5T32 GM008334)Fonds de la recherche en sante du Quebec (Master's Training Award

The Physics of the B Factories

This work is on the Physics of the B Factories. Part A of this book contains a brief description of the SLAC and KEK B Factories as well as their detectors, BaBar and Belle, and data taking related issues. Part B discusses tools and methods used by the experiments in order to obtain results. The results themselves can be found in Part C

Limited dissemination of the wastewater treatment plant core resistome

Horizontal gene transfer is a major contributor to the evolution of bacterial genomes and can facilitate the dissemination of antibiotic resistance genes between environmental reservoirs and potential pathogens. Wastewater treatment plants (WWTPs) are believed to play a central role in the dissemination of antibiotic resistance genes. However, the contribution of the dominant members of the WWTP resistome to resistance in human pathogens remains poorly understood. Here we use a combination of metagenomic functional selections and comprehensive metagenomic sequencing to uncover the dominant genes of the WWTP resistome. We find that this core resistome is unique to the WWTP environment, with <10% of the resistance genes found outside the WWTP environment. Our data highlight that, despite an abundance of functional resistance genes within WWTPs, only few genes are found in other environments, suggesting that the overall dissemination of the WWTP resistome is comparable to that of the soil resistome