Evidencia de la respuesta a un enterramiento a corto plazo de la fauna macrobentónica asociada a la fanerógama marina del Mediterráneo Cymodocea nodosa

Sedimentation and burial affect a variety of habitats worldwide, especially within coastal marine systems. In the Mediterranean, seagrasses like Cymodocea nodosa are commonly subjected to sedimentation and, although the response of C. nodosa has been documented, few studies have included macrofaunal responses. We used a manipulative field experiment to examine the effects of a single small-scale, pulse burial event on benthic invertebrate macrofauna. Burial did not affect the total abundance, richness, or diversity of higher taxa nor the live abundance or diversity after 5 days. However, live higher taxa richness decreased by day 3. After 5 days, such decrease reversed and partial recovery seemed to occur. Almost 2 months later, three of the buried plots remained with some additional sedimentation, but the other three had lost their sediment. We compared faunal metrics of buried plots between days 5 and 54 and found greater diversity on day 54, providing evidence that macrofaunal recovery apparent 5 days after burial seemed to persist. Although we likely underestimated the effects and can only be conservative with our conclusions, taken together our results suggest small-scale, pulse burial events can have some negative effects on the C. nodosa-associated benthic macrofaunal community, but the community can recover within a few weeks.La sedimentación y el enterramiento afectan a una gran variedad de hábitats en todo el mundo, especialmente en los sistemas costeros marinos. En el Mediterráneo, las fanerógamas marinas como Cymodocea nodosa suelen estar expuestas a la sedimentación y, a pesar de que la respuesta de C. nodosa ha sido documentada previamente, muy pocos estudios hacen referencia a cómo responde la macrofauna. Se ha usado un experimento manipulativo en el campo para examinar los efectos de un enterramiento súbito y puntual a pequeña escala sobre la fauna de invertebrados macrobentónicos. El enterramiento no afectó a la abundancia total, riqueza o diversidad de especies, ni tampoco a la abundancia o diversidad de individuos vivos 5 días después del enterramiento. Sin embargo, la riqueza de taxones vivos disminuyó a partir del día 3. Pasados 5 días, esa disminución revirtió y parece tener lugar una recuperación parcial. Casi 2 meses después, tres de las parcelas enterradas todavía presentaron algún tipo de sedimentación adicional, pero las otras tres habían perdido el sedimento. Se compararon las métricas de la fauna encontrada en los días 5 y 54 en las parcelas enterradas, y se encontró una alta diversidad para el día 54, evidenciando que la aparente recuperación que tuvo lugar 5 días después del enterramiento parece persistir. A pesar de que probablemente hemos subestimado los efectos y debemos ser conservadores con nuestras conclusiones, al examinar los resultados conjuntamente, sugieren que estos eventos repentinos y puntuales de enterramiento a pequeña escala pueden tener efectos negativos sobre la comunidad de fauna macrobentónica asociada a C. nodosa, la cual se recuperaría en unas pocas semanas

The role of state breastfeeding laws and programs on exclusive breastfeeding practice among mothers in the special supplemental nutrition program for Women, Infants, and Children (WIC)

Background: It is unclear if state laws supporting breastfeeding are associated with exclusive breastfeeding (EBF) practice among low-income mothers participating in the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC). The main objectives of our study were to assess the relationship between such laws and EBF among WIC-participating mothers and to assess whether this association varied by employment status. We also assessed how mother’s exposure to WIC breastfeeding consultation was associated with EBF. Methods: A cross-sectional study was conducted across seven WIC program locations (i.e., Georgia, Massachusetts, Nevada, Pennsylvania, Wisconsin, Wyoming, Chickasaw Nation) between July–August 2020. Data were collected using convenient sampling from each program location and surveys were administered electronically or on paper to WIC-participating mothers. We restricted our analysis to data from 1161 WIC-participating mothers with infants aged zero to five months old. Multivariable mixed models were used to estimate the relationship between our exposures of interest (i.e., number of laws supporting breastfeeding, employment-related breastfeeding laws, WIC breastfeeding consultation) and EBF, while controlling for potential confounders and accounting for clustering by program location. Effect modification by employment status was assessed on the additive and multiplicative scales. Results: Among WIC-participating mothers living in program locations with no employment-related breastfeeding laws, EBF was 26% less prevalent for employed mothers compared to unemployed mothers (adjusted prevalence ratios [aPR]: 0.74, 95% CI: 0.67,0.83). Among all mothers, a one-unit increase in laws supporting breastfeeding was not associated with EBF (aPR: 0.88, 95% CI: 0.71,1.10). However, among employed mothers, living in areas with more employment-related laws was associated with a higher prevalence of EBF (aPR: 1.43, 95% CI: 0.83, 2.44). Infants whose mothers received a WIC breastfeeding consultation had 33% higher prevalence of being exclusively breastfed compared to infants whose mothers did not receive a WIC breastfeeding consultation (aPR: 1.33, 95% CI: 1.05,1.70). Conclusions: Infants whose WIC-participating mothers were employed, were less likely to be exclusively breastfed, but our effect modification analyses showed that laws supporting breastfeeding at the workplace may promote EBF among employed women. EBF was more prevalent among mothers who received a WIC breastfeeding consultation compared to those who did not receive such a consultation

Analysis of shared heritability in common disorders of the brain

ience, this issue p. eaap8757 Structured Abstract INTRODUCTION Brain disorders may exhibit shared symptoms and substantial epidemiological comorbidity, inciting debate about their etiologic overlap. However, detailed study of phenotypes with different ages of onset, severity, and presentation poses a considerable challenge. Recently developed heritability methods allow us to accurately measure correlation of genome-wide common variant risk between two phenotypes from pools of different individuals and assess how connected they, or at least their genetic risks, are on the genomic level. We used genome-wide association data for 265,218 patients and 784,643 control participants, as well as 17 phenotypes from a total of 1,191,588 individuals, to quantify the degree of overlap for genetic risk factors of 25 common brain disorders. RATIONALE Over the past century, the classification of brain disorders has evolved to reflect the medical and scientific communities' assessments of the presumed root causes of clinical phenomena such as behavioral change, loss of motor function, or alterations of consciousness. Directly observable phenomena (such as the presence of emboli, protein tangles, or unusual electrical activity patterns) generally define and separate neurological disorders from psychiatric disorders. Understanding the genetic underpinnings and categorical distinctions for brain disorders and related phenotypes may inform the search for their biological mechanisms. RESULTS Common variant risk for psychiatric disorders was shown to correlate significantly, especially among attention deficit hyperactivity disorder (ADHD), bipolar disorder, major depressive disorder (MDD), and schizophrenia. By contrast, neurological disorders appear more distinct from one another and from the psychiatric disorders, except for migraine, which was significantly correlated to ADHD, MDD, and Tourette syndrome. We demonstrate that, in the general population, the personality trait neuroticism is significantly correlated with almost every psychiatric disorder and migraine. We also identify significant genetic sharing between disorders and early life cognitive measures (e.g., years of education and college attainment) in the general population, demonstrating positive correlation with several psychiatric disorders (e.g., anorexia nervosa and bipolar disorder) and negative correlation with several neurological phenotypes (e.g., Alzheimer's disease and ischemic stroke), even though the latter are considered to result from specific processes that occur later in life. Extensive simulations were also performed to inform how statistical power, diagnostic misclassification, and phenotypic heterogeneity influence genetic correlations. CONCLUSION The high degree of genetic correlation among many of the psychiatric disorders adds further evidence that their current clinical boundaries do not reflect distinct underlying pathogenic processes, at least on the genetic level. This suggests a deeply interconnected nature for psychiatric disorders, in contrast to neurological disorders, and underscores the need to refine psychiatric diagnostics. Genetically informed analyses may provide important "scaffolding" to support such restructuring of psychiatric nosology, which likely requires incorporating many levels of information. By contrast, we find limited evidence for widespread common genetic risk sharing among neurological disorders or across neurological and psychiatric disorders. We show that both psychiatric and neurological disorders have robust correlations with cognitive and personality measures. Further study is needed to evaluate whether overlapping genetic contributions to psychiatric pathology may influence treatment choices. Ultimately, such developments may pave the way toward reduced heterogeneity and improved diagnosis and treatment of psychiatric disorders

From Middens to Modern Estuaries, Oyster Shells Sequester Source-Specific Nitrogen

Oysters (Crassostrea virginica) were an important food resource for native peoples of the northern Gulf of Mexico, who deposited waste shells in middens. Nitrogen (N) stable isotopes (δ15N) in bivalve shells have been used as modern proxies for estuarine N sources because they approximate δ15N in suspended particulate matter. We tested the use of midden shell δ15N as a proxy for ancient estuarine N sources. We hypothesized that isotopic signatures in ancient shells from coastal Mississippi would differ from modern shells due to increased anthropogenic N sources, such as wastewater, through time. We decalcified shells using an acidification technique previously developed for modern bivalves, but modified to determine δ15N, δ13C, %N, and % organic C of these low-N, high-C specimens. The modified method resulted in the greatest percentage of usable data from midden shells. Our results showed that oyster shell δ15N did not significantly differ between ancient (500–2100 years old) and modern oysters from the same locations where the sites had undergone relatively little land-use change. δ15N values in modern shells, however, were positively correlated with water column nitrate concentrations associated with urbanization. When N content and total shell mass were combined, we estimated that middens sequestered 410–39,000 kg of relic N, buried at a rate of up to 5 kg N m−2 yr−1. This study provides a relatively simple technique to assess baseline conditions in ecosystems over long time scales by demonstrating that midden shells can be an indicator of pre-historic N source to estuariesand are a potentially significant but previously uncharacterized estuarine N sink

Bay Scallops: The High Resolution Canary in the Highly-Fertilized Coal Mine

As human populations increase in watersheds, the Peconic Bay estuary (NY) has experienced increased nitrogen (N) loads, resulting in eutrophication. Stable isotopes can be used as indicators of anthropogenic N sources such as wastewater and fertilizer, and potentially be used to track source-specific N inputs through time. We hypothesized that Peconic Bay scallops (Argopecten irridians) could be proxies for these environmental changes through assimilation of organic matter from surrounding water into their soft tissue and shells. We examined nitrogen stable isotopes (δ15N) from whole soft tissue and shell of scallops collected throughout the Peconic. A strong correlation was found between shell and soft tissue δ15N values, suggesting that scallop shells are reliable proxies for soft tissue. Scallops from sites with higher percentages of N from wastewater had enriched δ15N values, whereas sites with higher percentages of fertilizer N inputs had depleted δ15N values in scallop shells and tissues. Thus, no significant relationship was found among sites between total watershed N yield and shell or soft tissue δ15N. This study is among the first to demonstrate fertilizer-derived N being incorporated into bivalve shell, which has implications for tracking long-term fertilizer inputs to watersheds on an annual time scale using archival shells

Alcohol intake and invasive breast cancer risk by molecular subtype and race in the Carolina Breast Cancer Study

PURPOSE: Alcohol is an established breast cancer risk factor, but there is little evidence on whether the association differs between African Americans and whites. METHODS: Invasive breast cancers (n=1,795; 1,014 white, 781 African American) and age- and race-matched controls (n= 1,558; 844 white, 714 African American) from the Carolina Breast Cancer Study (Phases I–II) were used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) for pre-diagnosis drinks per week and breast cancer risk. RESULTS: African American controls reported lower alcohol intake than white controls across all age groups. Light drinking (0-≤2 per week) was more prevalent among African American controls. Moderate to heavy drinking was more prevalent in white controls. African Americans who reported drinking >7 drinks per week had an elevated risk compared to light drinkers [adjusted OR, 95% CI: 1.62 (1.03–2.54)]. A weaker association was observed among whites [adjusted OR, 95% CI: 1.20 (0.87–1.67)]. The association of >7 drinks per week with estrogen receptor negative [adjusted OR, 95% CI: 2.17 (1.25–3.75)] and triple negative [adjusted OR, 95% CI: 2.12 (1.12–4.04)] breast cancers was significant for African American, but not white women. We observed significantly elevated ORs for heavy intake at ages less than 25 and greater than 50 years of age for African American women only. We found no evidence of statistical interaction between alcohol intake with oral contraceptive use or smoking. CONCLUSIONS: Drinking more than 7 alcoholic beverages per week increased invasive breast cancer risk among white and African American women, with significant increases only among African American women. Genetic or environmental factors that differ by race may mediate the alcohol-breast cancer risk association