124 research outputs found
Plants lacking the main light-harvesting complex retain photosystem II macro-organization
Photosystem II (PSII) is a key component of photosynthesis, the process of converting sunlight into the chemical energy of life. In plant cells, it forms a unique oligomeric macrostructure in membranes of the chloroplasts. Several light-harvesting antenna complexes are organized precisely in the PSII macrostructure—the major trimeric complexes (LHCII) that bind 70% of PSII chlorophyll and three minor monomeric complexes—which together form PSII supercomplexes. The antenna complexes are essential for collecting sunlight and regulating photosynthesis, but the relationship between these functions and their molecular architecture is unresolved. Here we report that antisense Arabidopsis plants lacking the proteins that form LHCII trimers have PSII supercomplexes with almost identical abundance and structure to those found in wild-type plants. The place of LHCII is taken by a normally minor and monomeric complex, CP26, which is synthesized in large amounts and organized into trimers. Trimerization is clearly not a specific attribute of LHCII. Our results highlight the importance of the PSII macrostructure: in the absence of one of its main components, another protein is recruited to allow it to assemble and function
Quasi-classical approximation in vortex filament dynamics. Integrable systems, gradient catastrophe and flutter
Quasiclassical approximation in the intrinsic description of the vortex
filament dynamics is discussed. Within this approximation the governing
equations are given by elliptic system of quasi-linear PDEs of the first order.
Dispersionless Da Rios system and dispersionless Hirota equation are among
them. They describe motion of vortex filament with slow varying curvature and
torsion without or with axial flow. Gradient catastrophe for governing
equations is studied. It is shown that geometrically this catastrophe manifests
as a fast oscillation of a filament curve around the rectifying plane which
resembles the flutter of airfoils. Analytically it is the elliptic umbilic
singularity in the terminology of the catastrophe theory. It is demonstrated
that its double scaling regularization is governed by the Painleve' I equation.Comment: 25 pages, 5 figures, minor typos correcte
Identification of red high proper-motion objects in Tycho-2 and 2MASS catalogues using Virtual Observatory tools
Aims: With available Virtual Observatory tools, we looked for new M dwarfs in
the solar neighbourhood and M giants with high tangential velocities. Methods:
From an all-sky cross-match between the optical Tycho-2 and the near-infrared
2MASS catalogues, we selected objects with proper motions >50mas/yr and very
red V-Ks colours. For the most interesting targets, we collected
multi-wavelength photometry, constructed spectral energy distributions,
estimated effective temperatures and surface gravities from fits to atmospheric
models, performed time-series analysis of ASAS V-band light curves, and
assigned spectral types from low-resolution spectroscopy obtained with CAFOS at
the 2.2m Calar Alto telescope. Results: We got a sample of 59 bright red high
proper-motion objects, including fifty red giants, four red dwarfs, and five
objects reported in this work for the first time. The five new stars have
magnitudes V~10.8-11.3mag, reduced proper motions midway between known dwarfs
and giants, near-infrared colours typical of giants, and effective temperatures
Teff~2900-3400K. From our time-series analysis, we discovered a long secondary
period in Ruber 4 and an extremely long primary period in Ruber 6. With the
CAFOS spectra, we confirmed the red giant nature of Ruber 7 and 8, the last of
which seems to be one of the brightest metal-poor M giants ever identified.Comment: Accepted in Astronomy & Astrophysic
Widespread platinum anomaly documented at theYounger Dryas onset in North American sedimentary sequences
Previously, a large platinum (Pt) anomaly was reported in the Greenland ice sheet at the Younger Dryas
boundary (YDB) (12,800 Cal B.P.). In order to evaluate its geographic extent, fire-assay and inductively coupled plasma mass spectrometry (FA and ICP-MS) elemental analyses were performed on 11
widely separated archaeological bulk sedimentary sequences. We document discovery of a distinct Pt anomaly spread widely across North America and dating to the Younger Dryas (YD) onset. The apparent synchroneity of this widespread YDB Pt anomaly is consistent with Greenland Ice Sheet Project 2 (GISP2) data that indicated atmospheric input of platinum-rich dust. We expect the Pt anomaly to serve as a widely-distributed time marker horizon (datum) for identification and correlation of the onset of the YD climatic episode at 12,800 Cal B.P. This Pt datum will facilitate the dating and correlating of archaeological, paleontological, and paleoenvironmental data between sequences, especially those with limited age control
A new methodological contribution for the geodiversity assessment: applicability to Ceará State (Brazil)
The concept of geodiversity aggregates the abiotic elements of nature and promotes the geoconservation. The main objective of this work is to contribute to the upgrade of the method for the assessment and quantification of geodiversity proposed by Pereira et al. (2013). The method is based on the superposition of a regular grid of 12 × 12 km on different maps (lithology, geomorphology, soil, paleonthology, mineral and geological energy resources) at scales of 1:250,000 to 1:600,000. In addition to other up- grades, the water resources are regarded here as a new com- ponent to consider when quantifying geodiversity. The sum of these maps generated the quantitative Map of Geodiversity Indices and the Map of Geodiversity Assessment, ranging from very low to very high geodiversity. The analysis of the geodiversity map of the State of Ceará (Brazil) shows the applicability and advantage of this method, highlighting two regions with higher levels of geodiversity (Northwest and South) and another region with the lowest levels (Sertões Cearenses). The results also allowed the characterization of the State of Ceará concerning the individual components of the geodiversity, especially the water resources. Geodiversity indices and maps are comprehensive and user-friendly data in the territorial planning, considering the geodiversity either as a whole, or each of its components, especially the more sensi- tive such as fossil conservation, and water, mineral, and non- renewable energy resources management.The authors express their gratitude to the Brazilian
research fostering institution "Coordenação de Aperfeiçoamento de
Pessoal de Nível Superior" (CAPES) for awarding the Ciência Sem
Fronteiras (CsF) PhD scholarship that enabled this work. This work was
partially co-funded by the European Union through the European Regional
Development Fund, based on COMPETE 2020 (Programa Operacional da
Competitividade e Internacionalização), project ICT (UID/GEO/04683/
2013) with reference POCI-01-0145-FEDER-007690 and national funds
provided by Fundação para a Ciência e Tecnologia
Response of the Diatom Phaeodactylum tricornutum to Photooxidative Stress Resulting from High Light Exposure
The response of microalgae to photooxidative stress resulting from high light exposure is a well-studied phenomenon. However, direct analyses of photosystem II (PSII) D1 protein (the main target of photoinhibition) in diatoms are scarce. In this study, the response of the diatom model species Phaeodactylum tricornutum to short-term exposure to high light was examined and the levels of D1 protein determined immunochemically. Low light (LL) acclimated cells (40 µmol photons m−2 s−1) subjected to high light (HL, 1,250 µmol photons m−2 s−1) showed rapid induction of non-photochemical quenching (NPQ) and ca. 20-fold increase in diatoxanthin (DT) concentration. This resulted from the conversion of diadinoxanthin (DD) to DT through the activation of the DD-cycle. D1 protein levels under LL decreased about 30% after 1 h of the addition of lincomycin (LINC), a chloroplast protein synthesis inhibitor, showing significant D1 degradation and repair under low irradiance. Exposure to HL lead to a 3.2-fold increase in D1 degradation rate, whereas average D1 repair rate was 1.3-x higher under HL than LL, leading to decreased levels of D1 protein under HL. There were significant effects of both HL and LINC on P. tricornutum maximum quantum yield of PSII (Fv/Fm), showing a reduction of active PSII reaction centres. Partial recovery of Fv/Fm in the dark demonstrates the photosynthetic resilience of this diatom to changes in the light regime. P. tricornutum showed high allocation of total protein to D1 and an active D1-repair cycle to limit photoinhibition
Dynamic thylakoid stacking regulates the balance between linear and cyclic photosynthetic electron transfer
An Author Correction to this article was published on 29 May 2018 https://www.nature.com/articles/s41477-018-0163-4
http://eprints.whiterose.ac.uk/131699/
Upon transition of plants from darkness to light the initiation of photosynthetic linear electron transfer (LET) from H2O to NADP+ precedes the activation of CO2 fixation, creating a lag period where cyclic electron transfer (CET) around photosystem I (PSI) has an important protective role. CET generates ΔpH without net reduced NADPH formation, preventing overreduction of PSI via regulation of the cytochrome b 6 f (cytb 6 f) complex and protecting PSII from overexcitation by inducing non-photochemical quenching. The dark-to-light transition also provokes increased phosphorylation of light-harvesting complex II (LHCII). However, the relationship between LHCII phosphorylation and regulation of the LET/CET balance is not understood. Here, we show that the dark-to-light changes in LHCII phosphorylation profoundly alter thylakoid membrane architecture and the macromolecular organization of the photosynthetic complexes, without significantly affecting the antenna size of either photosystem. The grana diameter and number of membrane layers per grana are decreased in the light while the number of grana per chloroplast is increased, creating a larger contact area between grana and stromal lamellae. We show that these changes in thylakoid stacking regulate the balance between LET and CET pathways. Smaller grana promote more efficient LET by reducing the diffusion distance for the mobile electron carriers plastoquinone and plastocyanin, whereas larger grana enhance the partition of the granal and stromal lamellae plastoquinone pools, enhancing the efficiency of CET and thus photoprotection by non-photochemical quenching
Determination of antioxidant compounds in foodstuff
© 2017 Scrivener Publishing LLC. Phenolic compounds, vitamins and carotenoids are naturally found in different foodstuff. These antioxidant compounds play an important role in human health and are of interest for the food, pharmaceutical and cosmeceutical industries. Modern chromatographic and spectrometric techniques have made analysis easier than ever before, but their success depends on the extraction method used. In fact, the different antioxidants are identifified using chromatographic techniques coupled to diff erent specififi c detectors according to the characteristics of each molecule. Beyond their well-known health-promoting effects, antioxidant molecules can also be used to functionalize or preserve the freshness, nutritive value, flflavor and color of foodstuff s, which justify their incorporation into several matrices. In this chapter, the most common antioxidant compounds in foodstuff will be described, as well as the methodologies involved in their extraction, separation, identifification and quantifification. The bioactive properties and industrial applications of these compounds through innovative techniques will also be taken into account.info:eu-repo/semantics/publishedVersio
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