Characterization of Cre recombinase models for the study of adipose tissue

The study of adipose tissue in vivo has been significantly advanced through the use of genetic mouse models. While the aP2-CreBI and aP2-CreSalk lines have been widely used to target adipose tissue, the specificity of these lines for adipocytes has recently been questioned. Here we characterize Cre recombinase activity in multiple cell populations of the major adipose tissue depots of these and other Cre lines using the membrane-Tomato/membrane-GFP (mT/mG) dual fluorescent reporter. We find that the aP2-CreBI and aP2-CreSalk lines lack specificity for adipocytes within adipose tissue, and that the aP2-CreBI line does not efficiently target adipocytes in white adipose depots. Alternatively, the Adiponectin-CreERT line shows high efficiency and specificity for adipocytes, while the PdgfRα-CreERUCL and PdgfRα-CreERJHU lines do not efficiently target adipocyte precursor cells in the major adipose depots. Instead, we show that the PdgfRα-Cre line is preferable for studies targeting adipocyte precursor cells in vivo

The effect of ecological harshness on perceptions of the ideal female body size: an experimental life history approach ☆ , ☆☆

Why do researchers regularly observe a relationship between ecological conditions and the heaviness of female body weight ideals? The current research uses insights from life history theory and female reproductive physiology to examine whether variability in female body ideals might emerge from the different life history strategies typically adopted by individuals living in harsh versus benign ecologies. Across three experiments, we demonstrate that women who were sensitized to faster life history strategies during childhood -as indexed by earlier menarche or lower childhood SES -respond to cues of ecological harshness by shifting away from the thin body weight typically favored by Western women toward a heavier female body ideal. Additionally, although men's perceptions of the ideal male body size did not shift in response to these cues, their perceptions of the ideal female body size did, with developmentally sensitized men also preferring a heavier female body size in the context of harsh ecologies

Genome-wide association study identifies six new loci influencing pulse pressure and mean arterial pressure.

Numerous genetic loci have been associated with systolic blood pressure (SBP) and diastolic blood pressure (DBP) in Europeans. We now report genome-wide association studies of pulse pressure (PP) and mean arterial pressure (MAP). In discovery (N = 74,064) and follow-up studies (N = 48,607), we identified at genome-wide significance (P = 2.7 × 10(-8) to P = 2.3 × 10(-13)) four new PP loci (at 4q12 near CHIC2, 7q22.3 near PIK3CG, 8q24.12 in NOV and 11q24.3 near ADAMTS8), two new MAP loci (3p21.31 in MAP4 and 10q25.3 near ADRB1) and one locus associated with both of these traits (2q24.3 near FIGN) that has also recently been associated with SBP in east Asians. For three of the new PP loci, the estimated effect for SBP was opposite of that for DBP, in contrast to the majority of common SBP- and DBP-associated variants, which show concordant effects on both traits. These findings suggest new genetic pathways underlying blood pressure variation, some of which may differentially influence SBP and DBP

Genetic variants in novel pathways influence blood pressure and cardiovascular disease risk.

Blood pressure is a heritable trait influenced by several biological pathways and responsive to environmental stimuli. Over one billion people worldwide have hypertension (≥140 mm Hg systolic blood pressure or  ≥90 mm Hg diastolic blood pressure). Even small increments in blood pressure are associated with an increased risk of cardiovascular events. This genome-wide association study of systolic and diastolic blood pressure, which used a multi-stage design in 200,000 individuals of European descent, identified sixteen novel loci: six of these loci contain genes previously known or suspected to regulate blood pressure (GUCY1A3-GUCY1B3, NPR3-C5orf23, ADM, FURIN-FES, GOSR2, GNAS-EDN3); the other ten provide new clues to blood pressure physiology. A genetic risk score based on 29 genome-wide significant variants was associated with hypertension, left ventricular wall thickness, stroke and coronary artery disease, but not kidney disease or kidney function. We also observed associations with blood pressure in East Asian, South Asian and African ancestry individuals. Our findings provide new insights into the genetics and biology of blood pressure, and suggest potential novel therapeutic pathways for cardiovascular disease prevention

Genetic associations at 53 loci highlight cell types and biological pathways relevant for kidney function.

Reduced glomerular filtration rate defines chronic kidney disease and is associated with cardiovascular and all-cause mortality. We conducted a meta-analysis of genome-wide association studies for estimated glomerular filtration rate (eGFR), combining data across 133,413 individuals with replication in up to 42,166 individuals. We identify 24 new and confirm 29 previously identified loci. Of these 53 loci, 19 associate with eGFR among individuals with diabetes. Using bioinformatics, we show that identified genes at eGFR loci are enriched for expression in kidney tissues and in pathways relevant for kidney development and transmembrane transporter activity, kidney structure, and regulation of glucose metabolism. Chromatin state mapping and DNase I hypersensitivity analyses across adult tissues demonstrate preferential mapping of associated variants to regulatory regions in kidney but not extra-renal tissues. These findings suggest that genetic determinants of eGFR are mediated largely through direct effects within the kidney and highlight important cell types and biological pathways

Attractive Female Romantic Partners Provide a Proxy for Unobservable Male Qualities

Previous research indicates that women find men more desirable when they appear to be desired by other women than in the absence of such cues—an effect referred to as female mate choice copying. Female mate choice copying is believed to emerge from a process whereby women use the presence of a man’s mate as a cue to his own quality. Here, we test this hypothesis explicitly by examining whether the desirability enhancement effect conferred on men by the presumed interest of an attractive female (a) emerges only when the female is described as being a man’s current romantic partner (Experiment 1) and (b) is mediated by women’s belief that men partnered to attractive women possess unobservable qualities that women value in their romantic partners (Experiment 2). The results of our two experiments found support for these hypotheses, shedding new light on the processes influencing human female mate choice copying

Mechanical Design of NESSI: New Mexico Tech Extrasolar Spectroscopic Survey Instrument

NESSI: the New Mexico Tech Extrasolar Spectroscopic Survey Instrument is a ground-based multi-object spectrograph that operates in the near-infrared. It will be installed on one of the Nasmyth ports of the Magdalena Ridge Observatory (MRO) 2.4-meter Telescope sited in the Magdalena Mountains, about 48 km west of Socorro-NM. NESSI operates stationary to the telescope fork so as not to produce differential flexure between internal opto-mechanical components during or between observations. An appropriate mechanical design allows the instrument alignment to be highly repeatable and stable for both short and long observation timescales, within a wide-range of temperature variation. NESSI is optically composed of a field lens, a field de-rotator, re-imaging optics, an auto-guider and a Dewar spectrograph that operates at LN2 temperature. In this paper we report on NESSI's detailed mechanical and opto-mechanical design, and the planning for mechanical construction, assembly, integration and verification