Protein kinases in the photic signaling of the mammalian circadian clock

Circadian clocks drive biological rhythms in physiology and behavior, providing a selective advantage by enabling organisms to synchronize to the 24 h environmental day. This process depends on lightdark transitions as the main signal that shifts the phase of the clock. In mammals, the light input reaches the master circadian clock in the hypothalamic suprachiasmatic nucleus through glutamatergic afferents from the retina, resulting in phase-shifts of the overt rhythms which depend on the time of the day at which light is applied, leading to changes in the activity of circadian core clock genes (i.e., Per1). This circadian gating of the synchronizing effect of light is dependent on the specific activation of signal transduction pathways involving several kinases acting on protein effectors. Protein phosphorylation is also an important regulatory mechanism essential for the generation and maintenance of circadian rhythms and plays a crucial role in the degradation and the appropriate turnover of PER proteins. In this work, we review the role of the main kinases implicated in the function of the master clock, with emphasis in those involved in circadian photic entrainment.Fil: Alessandro, Maria Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golombek, Diego Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Adaptation of Volunteer Functions Inventory in adults from Argentina

Los voluntarios son sujetos que participan en una organización sin retribución económica, colaborando con la mejora y el avance de una comunidad. El análisis de sus motivaciones resulta de interés en la psicología, pero no se dispone de un instrumento local para evaluarlas. El objetivode este estudio fue adaptar el Inventario de Funciones del Voluntariado (VFI). Se trabajó con 249 voluntarios de organizaciones no gubernamentales de diferentes provincias. Se recolectaron datos con una encuesta sociodemográfica y el VFI. Mediante un análisis factorial exploratorio utilizando el estimador minimum rank factor analysis con rotación oblimin directa, se aisló una estructura factorial de tres dimensiones (social y carrera, mejora y protección, valores y comprensión) que explicaban el 54.76 % de la varianza. La consistencia interna fue adecuada (α = .89/ .94). Como conclusión, se proporciona a profesionales e investigadores la adaptación del VFI a la población local, aportando evidencias de validez y confiabilidad.Volunteers are subjects who participate in an organization without financial compensation, contributing to the improvement and advancement of a community. The analysis of their motivations is of interest in Psychology; however, there is no local instrument to evaluate them. The aim of this study was to adapt the Volunteer Functions Inventory (VFI). A sample of 249 volunteers for non-governmental organizations from different provinces was studied. Data was collected through a sociodemographic survey and the VFI. An exploratory factorial analysis was performed using the Minimum Rank Factor Analysis estimator with Direct Oblimin rotation. A three-dimension factorial structure (social and career, improvement andprotection, values and comprehension) was obtained, which explained 54.76 % of the variance. Internal consistency was adequate (α = .89/.94). In conclusion, professionals and researchers are provided with an adaptation of the VFI to the local population, with favorable evidences of validity and reliability.Fil: Chiesa, Valeria Soledad. Universidad de Buenos Aires. Facultad de Psicología; ArgentinaFil: Stover, Juliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Psicología. Instituto de Investigaciones; Argentin

Adaptación del Inventario de Funciones del Voluntariado en adultos de Argentina

Volunteers are subjects who participate in an organization without financial compensation, contributing to the improvement and advancement of a community. The analysis of their motivations is of interest in Psychology; however, there is no local instrument to evaluate them. The aim of this study was to adapt the Volunteer Functions Inventory (VFI). A sample of 249 volunteers for non-governmental organizations from different provinces was studied. Data was collected through a sociodemographic survey and the VFI. An exploratory factorial analysis was performed using the Minimum Rank Factor Analysis estimator with Direct Oblimin rotation. A three-dimension factorial structure (social and career, improvement andprotection, values and comprehension) was obtained, which explained 54.76 % of the variance. Internal consistency was adequate (α = .89/.94). In conclusion, professionals and researchers are provided with an adaptation of the VFI to the local population, with favorable evidences of validity and reliability.Los voluntarios son sujetos que participan en una organización sin retribución económica, colaborando con la mejora y el avance de una comunidad. El análisis de sus motivaciones resulta de interés en la psicología, pero no se dispone de un instrumento local para evaluarlas. El objetivo de este estudio fue adaptar el Inventario de Funciones del Voluntariado (VFI). Se trabajó con 249 voluntarios de organizaciones no gubernamentales de diferentes provincias. Se recolectaron datos con una encuesta sociodemográfica y el VFI. Mediante un análisis factorial exploratorio utilizando el estimador minimum rank factor analysis con rotación oblimin directa, se aisló una estructura factorial de tres dimensiones (social y carrera, mejora y protección, valores y comprensión) que explicaban el 54.76 % de la varianza. La consistencia interna fue adecuada (α = .89/ .94). Como conclusión, se proporciona a profesionales e investigadores la adaptación del VFI a la población local, aportando evidencias de validez y confiabilidad

Movilidad social ascendente y experiencias de cambios en los estilos de vida en familias con origen en clase trabajadora

En la presente ponencia se exploran algunas dimensiones de los complejos procesos involucrados en los cambios de estilos de vida que experimentan familias que recorrieron trayectorias de ascenso social desde la clase trabajadora a clase media, que residen en el AMBA en la actualidad. Estas dimensiones son el entramado de relaciones sociales y ámbitos por los que circulan e interactúan las personas a lo largo de sus trayectorias sociales, como también códigos y valores que configuran actitudes y prácticas referentes al tiempo libre. En torno a estos ejes, el objetivo es lograr una primera aproximación al modo en que se van modificando e incorporando hábitos, preferencias y representaciones al estilo de vida. A partir de allí, se pretende detectar de que manera estas transformaciones favorecen la consolidación del ascenso social y en que medida operan como mecanismos de auto-percepción de clase y diferenciación social respecto de la familia de origen.Facultad de Humanidades y Ciencias de la Educació

Eficácia do acaricida maleato de estanho tri-n-butílico contra ácaros de poeira em carpetes, tecidos e espuma de colchão: padronização de metodologia

The aim of this study was to determine the effectiveness of the acaricide tri-n-butyl tin maleate, industrially applied to samples of carpets, mattress foam, and fabrics used for furniture upholstery, soft toys and shoe uppers. Approximately 100 adult house dust mites of the species Dermatophagoides pteronyssinus were inoculated into a Petri dish containing the sample (a piece of carpet, mattress foam, or fabric), treated with the acaricide, randomly collected. Mite-maintenance culture medium was added on top of each sample. After one, two, three, seven and 30 days of incubation at 25 ºC and 75% relative humidity, each dish was examined using a 40X stereoscopic microscope (40X). One hundred percent acaricide effectiveness was obtained in treated materials by the end of the 30th-day postinoculation period, under optimal conditions for mite maintenance.O objetivo do presente estudo foi verificar a eficácia do acaricida maleato de estanho tri-n-butílico, aplicado industrialmente em amostras de carpetes, tecidos de revestimentos de móveis e de calçados, assim como de espumas de colchão. Aproximadamente 100 ácaros adultos da espécie Dermatophagoides pteronyssinus foram inoculados em placa de Petri contendo a amostra (pedaço de colchão, tecido ou carpete), tratada com o produto acaricida, coletados aleatoriamente. Foi acrescentado sobre a amostra, meio de cultivo para a manutenção dos ácaros. Cada placa foi examinada após 1, 2, 3, 7 e 30 dias de incubação a 25 ºC e 75% de U.R.A. (umidade relativa do ar), sob microscópio estereoscópico com 40X de aumento. O acaricida maleato de estanho tri-n-butílico apresentou 100% de eficácia acaricida após 30 dias da aplicação, em condições ótimas para a manutenção dos ácaros

Atividade de tri-n-butyl tin maleate em carpetes contra Staphylococcus aureus e Aspergillus niger, verificada através de duas metodologias: Zona de Inibição (ZI) e Superfície de Inibição (Impressão)

The aim of the present study was to verify the activity of the Tri-N-Butyl Tin maleate compound against Staphylococcus aureus and Aspergillus niger, after its industrial application in 40 samples of carpets of different materials (polypropylene, polyester, polyamide and wool). The qualitative assays were performed through two methodologies: Inhibition Halo (HZ) and Inhibition of Surface (Print). The carpet with the product inhibited 100% of bacterial (Staphylococcus aureus) and fungi (Aspergillus niger) growth, under the conditions of this study. The microbial inhibition was higher in upper portion of carpets. The methodologies employed appear to be adequate to test the bactericide and fungicide activities of the Tri-N-Butyl Tin maleate. The print methodology confirmed the results obtained by the inhibition zone assay. Further studies using the same methodologies are needed to confirm our results.O objetivo do presente estudo foi verificar a atividade do composto maleato de estanho tri-n-butílico contra Staphylococcus aureus e Aspergillus niger, após sua aplicação industrial em 40 amostras de carpetes de diferentes materiais (polipropileno, poliéster, poliamida e lã). Os ensaios qualitativos foram realizados através de duas metodologias: Zona de Inibição (ZI) e Superfície de Inibição (Impressão). Os carpetes tratados com o produto apresentaram 100% de inibição de crescimento bacteriano (Staphylococcus aureus) e fúngico (Aspergillus niger), sob as condições desse estudo. A inibição de crescimento microbiano foi mais elevada na porção superior dos carpetes. As metodologias empregadas parecem ser adequadas para testar a atividade bactericida e fungicida do maleato de estanho tri-n-butílico. A metodologia de impressão confirmou os resultados obtidos no ensaio de zona de inibição. Estudos futuros utilizando as mesmas metodologias são necessários para confirmação destes dados

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Surgical site infection after gastrointestinal surgery in high-income, middle-income, and low-income countries: a prospective, international, multicentre cohort study

Background: Surgical site infection (SSI) is one of the most common infections associated with health care, but its importance as a global health priority is not fully understood. We quantified the burden of SSI after gastrointestinal surgery in countries in all parts of the world. Methods: This international, prospective, multicentre cohort study included consecutive patients undergoing elective or emergency gastrointestinal resection within 2-week time periods at any health-care facility in any country. Countries with participating centres were stratified into high-income, middle-income, and low-income groups according to the UN's Human Development Index (HDI). Data variables from the GlobalSurg 1 study and other studies that have been found to affect the likelihood of SSI were entered into risk adjustment models. The primary outcome measure was the 30-day SSI incidence (defined by US Centers for Disease Control and Prevention criteria for superficial and deep incisional SSI). Relationships with explanatory variables were examined using Bayesian multilevel logistic regression models. This trial is registered with ClinicalTrials.gov, number NCT02662231. Findings: Between Jan 4, 2016, and July 31, 2016, 13 265 records were submitted for analysis. 12 539 patients from 343 hospitals in 66 countries were included. 7339 (58·5%) patient were from high-HDI countries (193 hospitals in 30 countries), 3918 (31·2%) patients were from middle-HDI countries (82 hospitals in 18 countries), and 1282 (10·2%) patients were from low-HDI countries (68 hospitals in 18 countries). In total, 1538 (12·3%) patients had SSI within 30 days of surgery. The incidence of SSI varied between countries with high (691 [9·4%] of 7339 patients), middle (549 [14·0%] of 3918 patients), and low (298 [23·2%] of 1282) HDI (p < 0·001). The highest SSI incidence in each HDI group was after dirty surgery (102 [17·8%] of 574 patients in high-HDI countries; 74 [31·4%] of 236 patients in middle-HDI countries; 72 [39·8%] of 181 patients in low-HDI countries). Following risk factor adjustment, patients in low-HDI countries were at greatest risk of SSI (adjusted odds ratio 1·60, 95% credible interval 1·05–2·37; p=0·030). 132 (21·6%) of 610 patients with an SSI and a microbiology culture result had an infection that was resistant to the prophylactic antibiotic used. Resistant infections were detected in 49 (16·6%) of 295 patients in high-HDI countries, in 37 (19·8%) of 187 patients in middle-HDI countries, and in 46 (35·9%) of 128 patients in low-HDI countries (p < 0·001). Interpretation: Countries with a low HDI carry a disproportionately greater burden of SSI than countries with a middle or high HDI and might have higher rates of antibiotic resistance. In view of WHO recommendations on SSI prevention that highlight the absence of high-quality interventional research, urgent, pragmatic, randomised trials based in LMICs are needed to assess measures aiming to reduce this preventable complication