Um estudo comparativo da dinâmica microclimática em espaços adaptados para fins de conservação de acervos sob a ótica da sustentabilidade

The article retrieves and presents the main ways of combining the universe of cultural heritage conservation with the trends of sustainability and, especially, discusses the role of microclimate diagnostics of areas adapted to the museum function in the definition of preventive conservation strategies based on the principles of such movement (by some authors identified as slow conservation). This analysis is articulated with two case studies in which the dynamic behavior of physical-environmental parameters (temperature and humidity) collected during a oneyear interval in internal areas of two museums (Museu Paulista and Museu Casa da Xilogravura) are assessed, considering external climatic variations. The results and their interpretation allow corroborating the importance of diagnostics of conservation areas as a planning tool for preventive actions alternative to traditional approaches based on mechanized and automated environmental control. Moreover, they enable envisioning passive strategies that value existing potentials, minimize individual vulnerabilities and concretely contribute to the articulation with a sustainable world.Este artigo resgata e apresenta os principais caminhos de aproximação entre o universo da conservação de bens culturais e as tendências da sustentabilidade, discutindo particularmente o papel do diagnóstico microclimático de espaços adaptados à função museológica na definição das estratégias de conservação preventiva embasadas nos princípios de tal movimento (identificadas por alguns autores como slow conservation). Esta análise se articula com dois estudos de caso que avaliam os comportamentos dinâmicos de parâmetros físico-ambientais (temperatura e umidade) coletados durante o período de um ano em espaços internos do Museu Paulista da Universidade de São Paulo (USP) e do Museu Casa da Xilogravura, confrontados com as variações climáticas externas. Os resultados e suas interpretações permitem ressaltar a importância do diagnóstico dos espaços de conservação, enquanto ferramenta de planejamento de ações preventivas alternativas às tradicionais abordagens de controle ambiental mecanizado e automatizado, e vislumbrar estratégias passivas que valorizem potencialidades existentes, minimizem vulnerabilidades isoladas e contribuam concretamente para a articulação com um mundo sustentável

Comparacion del nivel de microfiltracion marginal de cavidades clase y restauradas con vidrio ionomero modificado con resina en piezas dentarias de pacientes adultos mayores y jovenes.

76 p.La meta de la odontología adhesiva es desarrollar materiales capaces de reemplazar y actuar como la estructura dental dura biológica perdida. La habilidad sellante y la fuerza de unión son las dos principales pruebas de laboratorio utilizadas para evaluar el comportamiento de los sistemas restaurativos-adhesivos. Aunque el sellado marginal de una restauración cervical en esmalte es confiable, los márgenes en dentina y cemento dificultan la obtención del mismo. Por esta razón es posible afirmar que, independientemente del material de restauración utilizado siempre existirá un espacio entre restauración y cavidad preparada. A partir de ello, es posible deducir que la eliminación completa de microfiltración jamás se logrará en los márgenes cervicales de cemento y dentina. La edad tiene un efecto directo sobre los tejidos dentarios. Ya es sabido que ellos envejecen de igual manera que los demás tejidos del cuerpo humano. Dicha situación, podría tener implicancia directa en la terapia de piezas dentarias envejecidas y pacientes adultos mayores, así como en la elección del material restaurador a utilizar. El propósito de este estudio in Vitro fue comparar los niveles de microfiltración marginal de cavidades clase V restauradas con vidrio ionómero modificado con resina en piezas dentarias extraídas de pacientes adultos mayores y pacientes jóvenes. Se realizaron cavidades clase V con forma de cuña en 26 piezas dentarias divididas en dos grupos, uno correspondiente a dientes de pacientes adultos mayores (n =13) y otro a dientes de pacientes jóvenes (n =13). Las cavidades fueron restauradas con vidrio ionómero modificado con resina, para luego ser sometidas a termociclado y, posteriormente, a cargas cíclicas. Cinco especimenes se perdieron en este proceso lo que representa un 19,23% de la muestra. A continuación, la muestra se mantuvo en una tinción de azul de metileno para finalmente ser seccionada y analizada bajo microscopía óptica. Se analizaron un total de 42 hemisecciones de las piezas dentarias, 18 correspondieron a piezas dentarias de adultos mayores (Grupo A) y 24 a piezas dentarias de pacientes jóvenes (Grupo B). Los resultados de microfiltración marginal se analizaron con la prueba de Mann- Whitney y Kruskal-Wallis, con una significancia estadística de 95 %. No hubo diferencia estadísticamente significativa en los niveles de microfiltración marginal entre los dos grupos analizados (p = 0,592). A partir de lo anterior es posible concluir que no existe diferencia estadísticamente significativa en los valores de microfiltración marginal en cavidades clase V restauradas con vidrio ionómero modificado con resina al comparar piezas dentarias de pacientes adultos mayores y jóvenes. No obstante, se observó una tendencia a presentar mayor proporción de especimenes con niveles de microfiltración 0 y 1 en el grupo B (Joven), y mayor proporción de especimenes con niveles de microfiltración 2 y 3 en el grupo A (Adultos Mayores)

Expression of calcium-binding proteins and selected neuropeptides in the human, chimpanzee, and crab-eating macaque claustrum

The claustrum is present in all mammalian species examined so far and its morphology, chemoarchitecture, physiology, phylogenesis and ontogenesis are still a matter of debate. Several morphologically distinct types of immunostained cells were described in different mammalian species. To date, a comparative study on the neurochemical organization of the human and non-human primates claustrum has not been fully described yet, partially due to technical reasons linked to the postmortem sampling interval. The present study analyze the localization and morphology of neurons expressing parvalbumin (PV), calretinin (CR), NPY, and somatostatin (SOM) in the claustrum of man (# 5), chimpanzee (# 1) and crab-eating monkey (# 3). Immunoreactivity for the used markers was observed in neuronal cell bodies and processes distributed throughout the anterior-posterior extent of human, chimpanzee and macaque claustrum. Both CR- and PV-immunoreactive (ir) neurons were mostly localized in the central and ventral region of the claustrum of the three species while SOM- and NPY-ir neurons seemed to be equally distributed throughout the ventral-dorsal extent. In the chimpanzee claustrum SOM-ir elements were not observed. No co-localization of PV with CR was found, thus suggesting the existence of two non-overlapping populations of PV and CR-ir interneurons. The expression of most proteins (CR, PV, NPY), was similar in all species. The only exception was the absence of SOM-ir elements in the claustrum of the chimpanzee, likely due to species specific variability. Our data suggest a possible common structural organization shared with the adjacent insular region, a further element that emphasizes a possible common ontogeny of the claustrum and the neocortex

Renal amyloid‐A amyloidosis in cats: Characterization of proteinuria and biomarker discovery, and associations with kidney histology

BackgroundAmyloid A (AA) amyloidosis is a protein misfolding disease arising from serum amyloid A (SAA). Systemic AA amyloidosis recently was shown to have a high prevalence in shelter cats in Italy and was associated with azotemia and proteinuria.ObjectivesInvestigate urine protein profiles and diagnostic biomarkers in cats with renal AA amyloidosis.AnimalsTwenty‐nine shelter cats.MethodsCase‐control study. Cats with renal proteinuria that died or were euthanized between 2018 and 2021 with available necropsy kidney, liver and spleen samples, and with surplus urine collected within 30 days before death, were included. Histology was used to characterize renal damage and amyloid amount and distribution; immunohistochemistry was used to confirm AA amyloidosis. Urine protein‐to‐creatinine (UPC) and urine amyloid A‐to‐creatinine (UAAC) ratios were calculated, and sodium dodecyl sulfate‐agarose gel electrophoresis (SDS‐AGE) and liquid chromatography‐mass spectrometry (LC‐MS) of proteins were performed.ResultsTwenty‐nine cats were included. Nineteen had AA amyloidosis with renal involvement. Cats with AA amyloidosis had a higher UPC (median, 3.9; range, 0.6‐12.7 vs 1.5; 0.6‐3.1; P = .03) and UAAC ratios (median, 7.18 × 10$^{−3}$; range, 23 × 10$^{−3}$‐21.29 × 10$^{−3}$ vs 1.26 × 10$^{−3}$; 0.21 × 10$^{−3}$‐6.33 × 10$^{−3}$; P = .04) than unaffected cats. The SDS‐AGE identified mixed‐type proteinuria in 89.4% of cats with AA amyloidosis and in 55.6% without AA amyloidosis (P = .57). The LC‐MS identified 63 potential biomarkers associated with AA amyloidosis (P < .05). Among these, urine apolipoprotein C‐III was higher in cats with AA amyloidosis (median, 1.38 × 10$^{7}$; range, 1.85 × 10$^{5}$‐5.29 × 10$^{7}$ vs 1.76 × 10$^{6}$; 0.0 × 10$^{0}$‐1.38 × 10$^{7}$; P = .01). In the kidney, AA‐amyloidosis was associated with glomerulosclerosis (P = .02) and interstitial fibrosis (P = .05).Conclusions and Clinical ImportanceRenal AA amyloidosis is associated with kidney lesions, increased proteinuria and increased urine excretion of SAA in shelter cats. Additional studies are needed to characterize the role of lipid transport proteins in the urine of affected cats

Pheochromocytoma in dogs undergoing adrenalectomy

Pheochromocytoma is frequent in dogs and carries a guarded prognosis. Current histological criteria may not predict malignant behavior in dogs, similar to humans. In humans, characterization of tumors has been refined using the pheochromocytoma of the adrenal gland scaled score (PASS) and by immunohistochemistry. The study aim was to investigate PASS and immunohistochemical markers used in humans in 24 dogs with pheochromocytoma that underwent adrenalectomy. Dogs with pheochromocytomas were reviewed and tumors collected. Histological sections were evaluated to apply the PASS and were single-labeled for chromogranin A, Ki-67, COX-2, p53, BCL-2, c-erbB-2, vascular endothelial growth factor, and S100. Survival, age, and vascular and capsular invasion were compared for PASS and immunohistochemical markers; results of PASS were also compared for each marker. Associations between markers were tested. PASS and immunohistochemical markers did not differ for survival, age, and vascular and capsular invasion. Tumors showing BCL-2 expression in >50% cells had lower PASS than those with lower expression (PASS: 7 ± 2 vs 9 ± 2; P = .011). Tumors positive for S100 had higher PASS than those that were negative (PASS: 10 ± 2 vs 7 ± 2; P = .001). Results of the different markers were not associated. In conclusion, in the context of canine pheochromocytoma, PASS and the selected immunohistochemical markers are not associated with survival, age, or vascular or capsular invasion. The higher PASS in S100-positive tumors may indicate that pheochromocytomas developing morphologic changes acquire S100 expression. The significance of lower PASS in tumors with elevated BCL-2 expression is uncertain. Overall, the use of PASS and the present immunohistochemical markers may not be useful in dogs with pheochromocytoma

Circulating Cell-Free DNA in Dogs with Mammary Tumors: Short and Long Fragments and Integrity Index

Circulating cell-free DNA (cfDNA) has been considered an interesting diagnostic/prognostic plasma biomarker in tumor-bearing subjects. In cancer patients, cfDNA can hypothetically derive from tumor necrosis/apoptosis, lysed circulating cells, and some yet unrevealed mechanisms of active release. This study aimed to preliminarily analyze cfDNA in dogs with canine mammary tumors (CMTs). Forty-four neoplastic, 17 non-neoplastic disease-bearing, and 15 healthy dogs were recruited. Necrosis and apoptosis were also assessed as potential source of cfDNA on 78 CMTs diagnosed from the 44 dogs. The cfDNA fragments and integrity index significantly differentiated neoplastic versus non-neoplastic dogs (P<0.05), and allowed the distinction between benign and malignant lesions (P<0.05). Even if without statistical significance, the amount of cfDNA was also affected by tumor necrosis and correlated with tumor size and apoptotic markers expression. A significant (P<0.01) increase of Bcl-2 in malignant tumors was observed, and in metastatic CMTs the evasion of apoptosis was also suggested. This study, therefore, provides evidence that cfDNA could be a diagnostic marker in dogs carrying mammary nodules suggesting that its potential application in early diagnostic procedures should be further investigated

OS BENEFÍCIOS DA ATIVIDADE FÍSICA PARA O TRATAMENTO DO TRANSTORNO DE ANSIEDADE GENERALIZADA E DEPRESSÃO EM PACIENTES ADULTOS: UMA REVISÃO INTEGRATIVA

Identificar na literatura científica as características benefícas dos impactos da atividade física para o processo de controle e tratamento do Transtorno de Ansiedade Generalizada em pacientes adultos. Método: revisão integrativa, cujos critérios de elegibilidade dos artigos foram: corresponder aos descritores “Transtorno de ansiedade”; “Ansiedade” e “Atividade física”, ser artigo de pesquisa; estar nos idioma português, inglês e espanhol; estar disponível eletronicamente nas bases de dados BVS (Biblioteca Virtual em Saúde) e as fontes de dados Scientific Eletronic Library onLine (SciELO) e Brazilian Journal of Health Review (BJHR), ano de publicação referente ao período de 2013 a 2023. Resultados: encontraram-se 08 publicações expressivas. A análise ocorreu mediante leituras analíticas e interpretativas. Considerações finais: A importância da atividade física para o tratamento da TAB são promissoras e acenam para a utilização de exercícios físicos aeróbios como auxiliares das terapêuticas tradicionais.https://revistaft.com.br/os-beneficios-da-atividade-fisica-para-o-tratamento-do-transtorno-de-ansiedade-generalizada-e-depressao-em-pacientes-adultos-uma-revisao-integrativa

Forward-central two-particle correlations in p-Pb collisions at root s(NN)=5.02 TeV

Two-particle angular correlations between trigger particles in the forward pseudorapidity range (2.5 2GeV/c. (C) 2015 CERN for the benefit of the ALICE Collaboration. Published by Elsevier B. V.Peer reviewe

Event-shape engineering for inclusive spectra and elliptic flow in Pb-Pb collisions at root(NN)-N-S=2.76 TeV

Peer reviewe