4,306 research outputs found
The use of light-emitting diodes to stimulate mitochondrial function and liver regeneration of partially hepatectomized rats
Efeito do extrato aquoso da Hyptis pectinata na regeneração hepática após hepatectomia parcial em ratos
Objetivo: Este estudo foi realizado com o objetivo de verificar o efeito do extrato aquoso da Hyptis pectinata na regeneração
hepática bem como nos níveis das enzimas séricas (AST, ALT e γ-GT) após hepatectomia parcial de 67% em ratos.
Métodos: AST, ALT e γ-GT, foram determinadas pelo método cinético utilizando um espectrofotômetro (Modelo E2250-
CELM). A regeneração hepática foi avaliada por imunohistoquímica (PCNA). Resultados: O pré-tratamento oral de 100 mg/
kg foi realizado durante 4 dias e causou aumento na regeneração hepática O pré-tratamento oral com 200 mg/kg diminuiu
significativamente os níveis de AST quando comparado com o grupo submetido ao pré-tratamento com água destilada. As
demais enzimas avaliadas não apresentaram diferenças quando comparadas entre os grupos estudados. Conclusão: O
presente estudo mostra que o extrato aquoso da Hyptis pectinata, numa concentração de 100 mg/kg possui alguma
atividade biológica estimulando a regeneração hepática e causando também um leve efeito hepatoprotetor numa
concentração de 200 mg/kg. _________________________________________________________________________________________ ABSTRACT: Purpose: This study was carried out to assess the effects of the aqueous extract of Hyptis pectinata leaves on liver
regeneration and on serum enzymes (AST, ALT and γ-GT) after 67% partial hepatectomy in rats. Methods: AST, ALT and
γ-GT, were determined by conventional procedures using a spectrophotometer (Model E2250-CELM). Liver regeneration
was evaluated by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). Results:Oral pretreatment
during 4 days at 100 mg/kg increased liver regeneration index. At 200 mg/kg, AST level was statistically decreased in
comparison to the group submited to distilled water administration. The other enzymes assessed disclosed no difference
when all groups were compared. Conclusion: The present study shows that the aqueous extract of Hyptis pectinata
leaves contains some biological active principles that stimulate liver regeneration at 100 mg/kg and cause slight hepatic
protection at 200 mg/kg
Uso de fontes de nitrogênio visando retardar a senescência em girassol ornamental
Ornamental sunflowers present senescence of the basal leaves, which is a negative factor from the viewpoint of commercialization. Even though being the fourth in production of grains in the world, the sunflower has some genotypes that are used with ornamental purpose for pot and cut flower. The study aimed to evaluate the effect of different nitrogen sources on some parameters of growth and development, and on the retardation of the senescence of the basal leaves in pot sunflower. The experiment was carried out with the variety cv. Sunflower F1 Sumbrite Supreme, planted in pots with a volume of 1,3 L, under controlled cultivation, at the Department of Vegetal Production of the State University of the North of Paraná, Luiz Meneghel Campus, in Bandeirantes (Paraná, Brazil). The experimental design was entirely in randomized blocks, with seven treatments and five repetitions. The pots had been filled with 50% of the commercial substratum Plantmax® and 50% of earthworm castings. The following treatments were done: control, fertilizer Super N ®, sulfamide®, urea, ammonium nitrate, ammonium sulphate and calcium nitrate at 100 mg L-1 of N, in two weekly applications, with 100 mL each pot. Two evaluations were performed, one at 30 days after planting (DAP), when the number of leaves and plant height (cm) were examined, and another at harvest, when the number of leaves, plant height, diameter of inflorescences and number of days between harvest and senescence of inflorescences where examined. There were no significant differences between the nitrogen sources used in the experiment, which allows the conclusion that all sources of nitrogen fertilizers examined in the experiment can be used to improve the quality of sunflower plants. O girassol ornamental apresenta senescência das folhas basais que é um fator negativo do ponto de vista da comercialização, mesmo sendo a quarta oleaginosa em produção de grãos no mundo, alguns genótipos são usados com finalidade ornamental para flor de corte e de vaso. O objetivo do trabalho foi o de avaliar o efeito de diferentes fontes de nitrogênio sobre alguns parâmetros de crescimento, desenvolvimento e no retardamento da senescência das folhas basais em girassol de vaso. O experimento foi conduzido em vaso, com volume de 1,3 L sob cultivo protegido, no Departamento de Produção Vegetal da Universidade Estadual do Norte do Paraná, Campus Luiz Meneghel, município de Bandeirantes–PR, utilizando do girassol cv. Sunflower F1 Sumbrite Supreme. O delineamento experimental foi em blocos ao acaso, composto por sete tratamentos com cinco repetições. Os vasos foram preenchidos com 50% de substrato comercial Plantmax ® e 50% de húmus de minhoca. Os tratamentos foram: testemunha (adubação realizada pelo produtor), adubo Super N ®, sulfammo®, uréia, nitrato de amônio, sulfato de amônio e nitrato de cálcio na dose 100 mg L-1 de N, em duas aplicações semanais, com volume de 100 mL por vaso. Foram realizadas duas avaliações, uma aos 30 dias após plantio (DAP), sendo avaliado o número de folhas e altura plantas (cm) e outra na colheita, quando os capítulos estavam totalmente abertos, sendo avaliados o número de folhas, altura de plantas, diâmetro das inflorescências e número de dias entre a colheita e a senescência das inflorescências. Não houve diferença significativa entre as fontes de nitrogênio utilizadas, permitindo concluir que todas as fontes de adubos nitrogenadas avaliadas no experimento podem ser utilizadas na melhoria da qualidade das plantas de girassol
Efeito do extrato aquoso de Sida cordifolia na regeneração hepática após hepatectomia parcial
Introdução: O uso de plantas medicinais para o tratamento de patologias humanas tem aumentado em todo mundo. Muitas
delas são usadas por administração oral, e após a absorção podem afetar muitos órgãos. Objetivo: Esse estudo, tem como
objetivo verificar o efeito do extrato aquoso de Sida cordifolia, popularmente conhecida no Brasil como “malva-branca”,
na regeneração hepática. Métodos: Vinte ratos foram divididos em 4 grupos: controle, Sida 100, Sida 200 e Sida 400. Os
animais foram submetidos a administração oral de água destilada, 100, 200 e 400 mg/kg de extrato aquoso de Sida cordifolia,
respectivamente. Imediatamente após, foi realizada hepatectomia parcial 67%. Vinte quatro horas após, os fígados foram
removidos. A regeneração hepática foi avaliada por imunohistoquímica (PCNA), usando o anticorpo monoclonal PC-10.
Resultados: Os grupos Sida100 e Sida200 mostraram índices de regeneração hepática maiores que o grupo controle
(p<0.001 e p<0.05, respectivamente). Conclusão: O extrato aquoso de Sida cordifolia estimula a regeneração hepática
após hepatectomia parcial a 67% em ratos. _________________________________________________________________________________________ ABSTRACT: Purpose: The use of medicinal plants for the treatment of human diseases has increased worldwide. Many of them are
used by oral administration and, after absorption, may affect many organs. Therefore, this study aimed at assessing the
effects of the aqueous extract of Sida cordifolia leaves, popularly known in Brazil as “malva-branca”, on liver regeneration.
Methods: Twenty rats were divided into four groups: control, Sida100, Sida200 and Sida400 groups. All animals were
submitted to oral administration of distilled water, 100, 200 and 400 mg/kg of the aqueous extract of Sida cordifolia,
respectively. Immediately after this, they underwent 67% partial hepatectomy. Twenty four hours later, their livers were
removed. Hepatic regeneration was assessed by immunohistochemical staining for proliferating cell nuclear antigen
(PCNA) using the PC-10 monoclonal antibody. Results: Sida100 and Sida200 groups disclosed higher liver regeneration
indices than control group (p<0.001 and p<0.05, respectively). Conclusion: The aqueous extract of Sida cordifolia
stimulates liver regeneration after 67% partial hepatectomy in rats
Influence of glutamine on morphological and functional changes of liver in the presence of extrahepatic biliary obstruction in rats
Application of Different Advanced Oxidation Processes for the Degradation of Organic Pollutants
Genome of the Avirulent Human-Infective Trypanosome—Trypanosoma rangeli
Background: Trypanosoma rangeli is a hemoflagellate protozoan parasite infecting humans and other wild and domestic mammals across Central and South America. It does not cause human disease, but it can be mistaken for the etiologic agent of Chagas disease, Trypanosoma cruzi. We have sequenced the T. rangeli genome to provide new tools for elucidating the distinct and intriguing biology of this species and the key pathways related to interaction with its arthropod and mammalian hosts. Methodology/Principal Findings: The T. rangeli haploid genome is ,24 Mb in length, and is the smallest and least repetitive trypanosomatid genome sequenced thus far. This parasite genome has shorter subtelomeric sequences compared to those of T. cruzi and T. brucei; displays intraspecific karyotype variability and lacks minichromosomes. Of the predicted 7,613 protein coding sequences, functional annotations could be determined for 2,415, while 5,043 are hypothetical proteins, some with evidence of protein expression. 7,101 genes (93%) are shared with other trypanosomatids that infect humans. An ortholog of the dcl2 gene involved in the T. brucei RNAi pathway was found in T. rangeli, but the RNAi machinery is non-functional since the other genes in this pathway are pseudogenized. T. rangeli is highly susceptible to oxidative stress, a phenotype that may be explained by a smaller number of anti-oxidant defense enzymes and heatshock proteins. Conclusions/Significance: Phylogenetic comparison of nuclear and mitochondrial genes indicates that T. rangeli and T. cruzi are equidistant from T. brucei. In addition to revealing new aspects of trypanosome co-evolution within the vertebrate and invertebrate hosts, comparative genomic analysis with pathogenic trypanosomatids provides valuable new information that can be further explored with the aim of developing better diagnostic tools and/or therapeutic targets
Usefulness of PCR-based assays to assess drug efficacy in Chagas disease chemotherapy: value and limitations
One major goal of research on Chagas disease is the development of effective chemotherapy to eliminate the infection from individuals who have not yet developed cardiac and/or digestive disease manifestations. Cure evaluation is the more complex aspect of its treatment, often leading to diverse and controversial results. The absence of reliable methods or a diagnostic gold standard to assess etiologic treatment efficacy still constitutes a major challenge. In an effort to develop more sensitive tools, polymerase chain reaction (PCR)-based assays were introduced to detect low amounts of Trypanosoma cruzi DNA in blood samples from chagasic patients, thus improving the diagnosis and follow-up evaluation after chemotherapy. In this article, I review the main problems concerning drug efficacy and criteria used for cure estimation in treated chagasic patients, and the work conducted by different groups on developing PCR methodologies to monitor treatment outcome of congenital infections as well as recent and late chronic T. cruzi infections
Measurements of fiducial and differential cross sections for Higgs boson production in the diphoton decay channel at s√=8 TeV with ATLAS
Measurements of fiducial and differential cross sections are presented for Higgs boson production in proton-proton collisions at a centre-of-mass energy of s√=8 TeV. The analysis is performed in the H → γγ decay channel using 20.3 fb−1 of data recorded by the ATLAS experiment at the CERN Large Hadron Collider. The signal is extracted using a fit to the diphoton invariant mass spectrum assuming that the width of the resonance is much smaller than the experimental resolution. The signal yields are corrected for the effects of detector inefficiency and resolution. The pp → H → γγ fiducial cross section is measured to be 43.2 ±9.4(stat.) − 2.9 + 3.2 (syst.) ±1.2(lumi)fb for a Higgs boson of mass 125.4GeV decaying to two isolated photons that have transverse momentum greater than 35% and 25% of the diphoton invariant mass and each with absolute pseudorapidity less than 2.37. Four additional fiducial cross sections and two cross-section limits are presented in phase space regions that test the theoretical modelling of different Higgs boson production mechanisms, or are sensitive to physics beyond the Standard Model. Differential cross sections are also presented, as a function of variables related to the diphoton kinematics and the jet activity produced in the Higgs boson events. The observed spectra are statistically limited but broadly in line with the theoretical expectations
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