Optimal Sizes of Dielectric Microspheres for Cavity QED with Strong Coupling

The whispering gallery modes (WGMs) of quartz microspheres are investigated for the purpose of strong coupling between single photons and atoms in cavity quantum electrodynamics (cavity QED). Within our current understanding of the loss mechanisms of the WGMs, the saturation photon number, n, and critical atom number, N, cannot be minimized simultaneously, so that an "optimal" sphere size is taken to be the radius for which the geometric mean, (n x N)^(1/2), is minimized. While a general treatment is given for the dimensionless parameters used to characterize the atom-cavity system, detailed consideration is given to the D2 transition in atomic Cesium (852nm) using fused-silica microspheres, for which the maximum coupling coefficient g/(2*pi)=750MHz occurs for a sphere radius a=3.63microns corresponding to the minimum for n=6.06x10^(-6). By contrast, the minimum for N=9.00x10^(-6) occurs for a sphere radius of a=8.12microns, while the optimal sphere size for which (n x N)^(1/2) is minimized occurs at a=7.83microns. On an experimental front, we have fabricated fused-silica microspheres with radii a=10microns and consistently observed quality factors Q=0.8x10^(7). These results for the WGMs are compared with corresponding parameters achieved in Fabry-Perot cavities to demonstrate the significant potential of microspheres as a tool for cavity QED with strong coupling.Comment: 12 pages, 14 figure

Survey of the prevalence of Salmonella species on laying hen farms in Kosovo

A survey on the prevalence of Salmonella (S.) species was carried out on 39 layer farms in Kosovo between April and September 2012. In total 367 samples, comprising feces, dust, eggs, and internal organs from dead birds, were investigated using bacteriological culture methods. Additionally, data on the location of the farm, the total number of birds on the farm, age of birds, and laying performance were collected. Salmonella were isolated from 38 samples obtained from 19 (49%) farms. The most common serovar identified was Salmonella enteritidis, found on 18 farms. The most common S. enteritidis phage type was PT29 followed by PT6, PT7, PT21, PT13a, PT8, PT14b, and PT4. One S. enteritidis isolate was not typable. Six farms had more than one phage type. Furthermore, serovar S. Bovismorbificans also was found in samples from 3 farms. Flock size or production stage was not associated with the probability of isolating Salmonella. The only flock factor found to be significantly associated was percent hen/day production: It was 2.8 times more likely to isolate Salmonella from flocks with production above 80% hen/day production compared to flocks producing at a lower level. Analysis of antimicrobial resistance patterns of 30 isolates revealed that all isolates were sensitive to gentamicin, ampicillin, sulphamethoxazole trimethoprim, and oxytetracycline, and 29 (97%) were sensitive to ciprofloxacin. All isolates showed intermediate resistance or were resistant to minocycline and cloxacillin. Twenty-six isolates (86%) had intermediate resistance to amoxicillin and 27 isolates (90%) were fully resistant to streptomycin. The present survey revealed a high prevalence of Salmonella enteritidis in layer flocks in Kosovo, indicating that table eggs have to be suspected as an important source of human salmone-llosis

Meta-analysis of exome array data identifies six novel genetic loci for lung function

Background: Over 90 regions of the genome have been associated with lung function to date, many of which have also been implicated in chronic obstructive pulmonary disease. Methods: We carried out meta-analyses of exome array data and three lung function measures: forced expiratory volume in one second (FEV1), forced vital capacity (FVC) and the ratio of FEV1 to FVC (FEV1/FVC). These analyses by the SpiroMeta and CHARGE consortia included 60,749 individuals of European ancestry from 23 studies, and 7,721 individuals of African Ancestry from 5 studies in the discovery stage, with follow-up in up to 111,556 independent individuals. Results: We identified significant (P<2·8x10-7) associations with six SNPs: a nonsynonymous variant in RPAP1, which is predicted to be damaging, three intronic SNPs (SEC24C, CASC17 and UQCC1) and two intergenic SNPs near to LY86 and FGF10. Expression quantitative trait loci analyses found evidence for regulation of gene expression at three signals and implicated several genes, including TYRO3 and PLAU. Conclusions: Further interrogation of these loci could provide greater understanding of the determinants of lung function and pulmonary disease

Geographical and temporal distribution of SARS-CoV-2 clades in the WHO European Region, January to June 2020

We show the distribution of SARS-CoV-2 genetic clades over time and between countries and outline potential genomic surveillance objectives. We applied three available genomic nomenclature systems for SARS-CoV-2 to all sequence data from the WHO European Region available during the COVID-19 pandemic until 10 July 2020. We highlight the importance of real-time sequencing and data dissemination in a pandemic situation. We provide a comparison of the nomenclatures and lay a foundation for future European genomic surveillance of SARS-CoV-2.Peer reviewe

An operational overview of the EXport processes in the ocean from RemoTe sensing (EXPORTS) northeast pacific field deployment

The goal of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) field campaign is to develop a predictive understanding of the export, fate, and carbon cycle impacts of global ocean net primary production. To accomplish this goal, observations of export flux pathways, plankton community composition, food web processes, and optical, physical, and biogeochemical (BGC) properties are needed over a range of ecosystem states. Here we introduce the first EXPORTS field deployment to Ocean Station Papa in the Northeast Pacific Ocean during summer of 2018, providing context for other papers in this special collection. The experiment was conducted with two ships: a Process Ship, focused on ecological rates, BGC fluxes, temporal changes in food web, and BGC and optical properties, that followed an instrumented Lagrangian float; and a Survey Ship that sampled BGC and optical properties in spatial patterns around the Process Ship. An array of autonomous underwater assets provided measurements over a range of spatial and temporal scales, and partnering programs and remote sensing observations provided additional observational context. The oceanographic setting was typical of late-summer conditions at Ocean Station Papa: a shallow mixed layer, strong vertical and weak horizontal gradients in hydrographic properties, sluggish sub-inertial currents, elevated macronutrient concentrations and low phytoplankton abundances. Although nutrient concentrations were consistent with previous observations, mixed layer chlorophyll was lower than typically observed, resulting in a deeper euphotic zone. Analyses of surface layer temperature and salinity found three distinct surface water types, allowing for diagnosis of whether observed changes were spatial or temporal. The 2018 EXPORTS field deployment is among the most comprehensive biological pump studies ever conducted. A second deployment to the North Atlantic Ocean occurred in spring 2021, which will be followed by focused work on data synthesis and modeling using the entire EXPORTS data set

The criminal justice voluntary sector: concepts and an agenda for an emerging field

This is the peer reviewed version of the following article: Tomczak, P. & Buck, G. (2019). The criminal justice voluntary sector: concepts and an agenda for an emerging field. Howard Journal of Crime and Justice, 58(3), which has been published in final form at https://doi.org/10.1111/hojo.12326. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.Volunteers and voluntary organisations play significant roles pervading criminal justice. They are key actors, with unrecognised potential to shore up criminal justice and/or collaboratively reshape social justice. Unlike public and for-profit agents, criminal justice volunteers and voluntary organisations (CJVVOs) have been neglected by scholars. We call for analyses of diverse CJVVOs, in national and comparative contexts. We provide three categories to highlight distinctive organising auspices, which hold across criminal justice: statutory volunteers, quasi-statutory volunteers and voluntary organisations. The unknown implications of these different forms of non-state, non-profit justice involvement deserve far greater attention from academics, policymakers and practitioners

Cytosolic adenylyl cyclase defines a unique signaling molecule in mammals.

Mammals have nine differentially regulated isoforms of G protein-responsive transmembrane-spanning adenylyl cyclases. We now describe the existence of a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or Forskolin regulation. Northern analysis indicates the gene encoding soluble adenylyl cyclase (sAC) is preferentially expressed in testis. As purified from rat testis cytosol, the active form of sAC appears to be a fragment derived from the full-length protein, suggesting a proteolytic mechanism for sAC activation. The two presumptive catalytic domains of sAC are closely related to cyanobacterial adenylyl cyclases, providing an evolutionary link between bacterial and mammalian signaling molecules. Adenylyl cyclase (AC) is the effector molecule of one of the most widely used signal transduction pathways. Its product, cAMP, mediates cellular responses to nutritional conditions and extracellular signals in organisms from prokaryotes to higher eukaryotes. In metazoans, a seemingly ubiquitous membrane-associated AC activity is encoded by a family of transmembrane adenylyl cyclases (tmACs) that mediate cellular responses to external stimuli. In mammals, nine distinct tmAC genes differing in their patterns of expression and regulatory properties have thus far been identified. Their catalytic activities are differentially regulated by G proteins and other signaling molecules in response to stimuli such as hormones and neurotransmitters (1, 2). In addition, another type of AC activity had been described in mammals. A soluble enzymatic activity was detected in cytosolic extracts from mammalian testis (3). Soluble AC activity appeared to be biochemically and chromatographically different from the tmACs and soluble guanylyl cyclases previously described in testis (4–6). Unlike the known tmACs, its biochemical activity depended on the divalent cation Mn2+ (3), was insensitive to G protein regulation (6), and displayed approximately 10-fold lower affinity for substrate, ATP (Km ≈1 mM) (4, 7, 8) than the tmACs (Km ≈100 μM) (9). Based on these studies, this soluble form of AC was predicted to be molecularly distinct from tmACs (8, 10). A soluble form of AC would define a novel means for generating cAMP and would imply that the second messenger could be generated at a distance from the membrane, closer to its required site of action. However, the molecular evidence confirming that soluble AC represents a distinct form of AC had been lacking. We now describe purification, molecular cloning, and functional expression of the cDNA encoding the soluble form of AC (sAC). The full-length cDNA predicts a protein of 187 kDa, whereas the catalytically active purified form of the enzyme is only 48 kDa, suggesting a proteolytic mechanism of activation for sAC. Two distinct regions within the catalytically active portion of sAC are very similar to catalytic portions of ACs from cyanobacteria and myxobacteria, providing a link between bacterial and mammalian signaling systems