SINFONI - Integral Field Spectroscopy at 50 milli-arcsecond resolution with the ESO VLT

SINFONI is an adaptive optics assisted near-infrared integral field spectrometer for the ESO VLT. The Adaptive Optics Module (built by the ESO Adaptive Optics Group) is a 60-elements curvature-sensor based system, designed for operations with natural or sodium laser guide stars. The near-infrared integral field spectrometer SPIFFI (built by the Infrared Group of MPE) provides simultaneous spectroscopy of 32 x 32 spatial pixels, and a spectral resolving power of up to 3300. The adaptive optics module is in the phase of integration; the spectrometer is presently tested in the laboratory. We provide an overview of the project, with particular emphasis on the problems encountered in designing and building an adaptive optics assisted spectrometer.Comment: This paper was published in Proc. SPIE, 4841, pp. 1548-1561 (2003), and is made available as an electronic reprint with permission of SPIE. Copyright notice added to first page of articl

Paleo-sea surface temperature calculations in the equatorial east Atlantic from Mg/Ca ratios in planktic foraminifera: A comparison to sea surface temperature estimates from UK'37, oxygen isotopes, and foraminiferal transfer function

We present two ∼270 kyr paleo-sea surface temperature (SST) records from the Equatorial Divergence and the South Equatorial Current derived from Mg/Ca ratios in the planktic foraminifer Globigerinoides sacculifer. The present study suggests that the magnesium signature of G. sacculifer provides a seasonal SST estimate from the upper ∼50 m of the water column generated during upwelling in austral low-latitude fall/winter. Common to both down-core records is a glacial-interglacial amplitude of ∼3°–3.5°C for the last climatic changes and lower Holocene and glacial oxygen isotope stage 2 temperatures compared with interglacial stage 5.5 and glacial stage 6 temperatures, respectively. The comparison to published SST estimates from alkenones, oxygen isotopes, and foraminiferal transfer function from the same core material pinpoints discrepancies and conformities between methods

Ice sheet–free West Antarctica during peak early Oligocene glaciation

One of Earth’s most fundamental climate shifts – the greenhouse-icehouse transition 34 Ma ago – initiated Antarctic ice-sheet build-up, influencing global climate until today. However, the extent of the ice sheet during the Early Oligocene Glacial Maximum (~33.7–33.2 Ma) that immediately followed this transition, a critical knowledge gap for assessing feedbacks between permanently glaciated areas and early Cenozoic global climate reorganization, is uncertain. Here, we present shallow-marine drilling data constraining earliest Oligocene environmental conditions on West Antarctica’s Pacific margin – a key region for understanding Antarctic ice sheet-evolution. These data indicate a cool-temperate environment, with mild ocean and air temperatures preventing West Antarctic Ice Sheet formation. Climate-ice sheet modeling corroborates a highly asymmetric Antarctic ice sheet, thereby revealing its differential regional response to past and future climatic change

E. coli Nissle 1917 Affects Salmonella Adhesion to Porcine Intestinal Epithelial Cells

BACKGROUND: The probiotic Escherichia coli strain Nissle 1917 (EcN) has been shown to interfere in a human in vitro model with the invasion of several bacterial pathogens into epithelial cells, but the underlying molecular mechanisms are not known. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we investigated the inhibitory effects of EcN on Salmonella Typhimurium invasion of porcine intestinal epithelial cells, focusing on EcN effects on the various stages of Salmonella infection including intracellular and extracellular Salmonella growth rates, virulence gene regulation, and adhesion. We show that EcN affects the initial Salmonella invasion steps by modulating Salmonella virulence gene regulation and Salmonella SiiE-mediated adhesion, but not extra- and intracellular Salmonella growth. However, the inhibitory activity of EcN against Salmonella invasion always correlated with EcN adhesion capacities. EcN mutants defective in the expression of F1C fimbriae and flagellae were less adherent and less inhibitory toward Salmonella invasion. Another E. coli strain expressing F1C fimbriae was also adherent to IPEC-J2 cells, and was similarly inhibitory against Salmonella invasion like EcN. CONCLUSIONS: We propose that EcN affects Salmonella adhesion through secretory components. This mechanism appears to be common to many E. coli strains, with strong adherence being a prerequisite for an effective reduction of SiiE-mediated Salmonella adhesion

A large-scale transcontinental river system crossed West Antarctica during the Eocene

Extensive ice coverage largely prevents investigations of Antarctica’s unglaciated past. Knowledge about environmental and tectonic development before large-scale glaciation, however, is important for understanding the transition into the modern icehouse world. We report geochronological and sedimentological data from a drill core from the Amundsen Sea shelf, providing insights into tectonic and topographic conditions during the Eocene (~44 to 34 million years ago), shortly before major ice sheet buildup. Our findings reveal the Eocene as a transition period from >40 million years of relative tectonic quiescence toward reactivation of the West Antarctic Rift System, coinciding with incipient volcanism, rise of the Transantarctic Mountains, and renewed sedimentation under temperate climate conditions. The recovered sediments were deposited in a coastal-estuarine swamp environment at the outlet of a >1500-km-long transcontinental river system, draining from the rising Transantarctic Mountains into the Amundsen Sea. Much of West Antarctica hence lied above sea level, but low topographic relief combined with low elevation inhibited widespread ice sheet formation

Gljivice kao sastavni dio bioaerosola u nastambama za muzne krave i nesilice konzumnih jaja

The air of animal dwellings can contain great amounts of bioaerosol composed of dust, bacteria, fungi, and endotoxins. The composition may depend on animal species, building construction, animal accommodation, and microclimate parameters, to name just a few factors. Pathogens contained may be a serious threat to animal and human health. The aim of our study was to analyse the fungi aerosol content in a stable housing dairy cows and in a coop for laying hens over the three autumn months of 2007. The air was sampled on Petri dishes with Sabouraud glucose agar. After laboratory treatment, we identifi ed the most common fungi. Their count in the stable ranged from 3.98x103 CFU m-3 to 5.11x104 CFU m-3 and in the coop from 6.89 x104 CFU m-3 to 1.13x105 CFU m-3. The difference between the two animal dwellings was statistically different at the level of p<0.05. In both dwellings, the most common were the fungi Aspergillus sp., Penicillium sp., and yeasts, followed by Cladosporium sp., Fusarium sp., Mucor sp., Scopulariopsis sp., Alternaria sp., and Rhizopus sp. Our results are entirely in line with values reported in literature and are at the lower end of the range. They call for further investigation that would eventually lead to setting air quality standards for animal dwellings and to developing reliable monitoring systems in order to ensure safe food and safe environment.U zraku nastambi za držanje životinja stvaraju se znatne količine bioaerosola. Njega čine prašina, bakterije, gljivice, endotoksini i plinovi. Brojnost im ovisi o građevinsko-tehničkim značajkama nastambi, naseljenosti životinjama, načinu držanja, temperaturno-vlažnim odnosima u staji i aktivnostima oko hranjenja, mužnje, skupljanja jaja i drugih poslova. Ove čestice, ako su patogene, mogu biti ozbiljna prijetnja za zdravlje ljudi. Mjerenja su obavljana u staji muznih krava te u objektu za nesilice, 2007. godine, tijekom tri jesenja mjeseca. Zrak je uzorkovan na Petrijeve zdjelice sa Sabouraudovim glukoznim agarom, uređajem MAS 100. Nakon obrade u laboratoriju, prema osnovnim i mikromorfološkim osobinama poraslih kolonija identifi cirani su najčešće zastupljeni rodovi gljivica. Srednja vrijednost broja gljivica u zraku staje za muzne krave kretala se od 3,98x103 CFU m-3 do 5,11x104 CFU m-3. Broj gljivica u zraku objekta za nesilice kretao se od 6,89x104 CFU m-3 do 1,13x105 CFU m-3. Ove vrijednosti statistički su se značajno razlikovale na razini p<0,05. U obje pretraživane nastambe najčešće su bili zastupljeni rodovi Aspergillus, Penicillium i kvasnice. U manjem postotku utvrđene su gljivice iz rodova Cladosporium sp., Fusarium sp., Mucor sp., Scopulariopsis sp., Alternaria sp. i Rhizopus sp. Rezultati ovih istraživanja o kvantitativnom i kvalitativnom sastavu gljivica u zraku pretraženih nastambi potpuno su u skladu s vrijednostima zabilježenim u literaturi te se nalaze na donjim granicama opisanih raspona. Utvrđeni broj i rodovi gljivica ukazuju na nužnost daljnjih istraživanja te potrebu postavljanja standardnih vrijednosti glede kvalitete zraka u nastambama za životinje, kao i razvoj vjerodostojnog sustava praćenja navedenih čimbenika, s ciljem stvaranja sigurne hrane i sigurnog okoliša

The climatic significance of Late Ordovician-early Silurian black

The Ordovician-Silurian transition (455-430 Ma) is characterized by repeated climatic perturbations, concomitant with major changes in the global oceanic redox state best exemplified by the periodic deposition of black shales. The relationship between the climatic evolution and the oceanic redox cycles, however, remains largely debated. Here, using an ocean-atmosphere general circulation model accounting for ocean biogeochemistry (MITgcm), we investigate the mechanisms responsible for the burial of organic carbon immediately before, during and right after the latest Ordovician Hirnantian (445-444 Ma) glacial peak. Our results are compared with recent sedimentological and geochemical data. We show that the late Katian time slice (445 Ma), typified by the deposition of black shales at tropical latitudes, represents an unperturbed oceanic state, with regional organic carbon burial driven by the surface primary productivity. During the Hirnantian, our experiments predict a global oxygenation event, in agreement with the disappearance of the black shales in the sedimentary record. This suggests that deep-water burial of organic matter may not be a tenable triggering factor for the positive carbon excursion reported at that time. Our simulations indicate that the perturbation of the ocean circulation induced by the release of freshwater, in the context of the post-Hirnantian deglaciation, does not sustain over sufficiently long geological periods to cause the Rhuddanian (444 Ma) oceanic anoxic event. Input of nutrients to the ocean, through increased continental weathering and the leaching of newly-exposed glaciogenic sediments, may instead constitute the dominant control on the spread of anoxia in the early Silurian