Evaluation of Mid-Year Review of 2014 Seminars: A case study of the CSIR-Water Research Institute, Ghana

Scientific seminars are organized to provide forum for participants to discuss and also bring into the public domain current activities of an organization. To ascertain whether the seminars are achieving its aim, evaluation is usually done to assess the effectiveness. It is against this background that Water Research Institute of the Council for Scientific and Industrial Research organized 2014 mid-year seminar to review its research activities as well as communicate findings of research undertaken by its scientist for the year under review. Hence, the main thrust of this paper is to evaluate the success of 2014 mid-year review seminar. The evaluation for the purposes of quality assurance and future organization of similar seminars were conducted using descriptive statistics analysis of the evaluation questionnaire. The results of the study revealed that 71% of the participants were males while 29% of them were females. Most of the respondents were of the view that the topics presented were relevant and the overall performance of the seminar was good as it was in line with the objectives of the institute, that is, to generate and provide scientific information. Keywords: CSIR-Water Research Institute, Scientific Seminars, Scientific Informatio

Silencing by nuclear matrix attachment distinguishes cell-type specificity: association with increased proliferation capacity

DNA loop organization by nuclear scaffold/matrix attachment is a key regulator of gene expression that may provide a means to modulate phenotype. We have previously shown that attachment of genes to the NaCl-isolated nuclear matrix correlates with their silencing in HeLa cells. In contrast, expressed genes were associated with the lithium 3,5-diiodosalicylate (LIS)-isolated nuclear scaffold. To define their role in determining phenotype matrix attached regions (MARs) on human chromosomes 14–18 were identified as a function of expression in a primary cell line. The locations of MARs in aortic adventitial fibroblast (AoAF) cells were very stable (r = 0.909) and 96% of genes attached at MARs are silent (P < 0.001). Approximately one-third of the genes uniquely expressed in AoAF cells were associated with the HeLa cell nuclear matrix and silenced. Comparatively, 81% were associated with the AoAF cell nuclear scaffold (P < 0.001) and expressed. This suggests that nuclear scaffold/matrix association mediates a portion of cell type-specific gene expression thereby modulating phenotype. Interestingly, nuclear matrix attachment and thus silencing of specific genes that regulate proliferation and maintain the integrity of the HeLa cell genome suggests that transformation may at least in part be achieved through aberrant nuclear matrix attachment

Determination of Formulation and Processing Factors Affecting Slowly Digestible Starch, Protein Digestibility and Antioxidant Capacity of Extruded Sorghum-Maize Composite Flour

High-temperature high-pressure extrusion of sorghum–maize composite flour, of potential for healthyfood manufacture, was investigated by factorial experimental design to determine the effect of level of sorghum in dry mix (15–60%); final barrel zone temperature (120–150 °C); total moisture in barrel(21.4–25.8%); total input rate (2.3–6.8 kg h -1); and screw speed (250–450 rpm) on extrudate slowlydigestible starch (SDS), phenolic content, antioxidant capacity, protein digestibility, density and expansion ratio. Extrudate SDS increased with increasing sorghum level and decreased as the barrel temperature increased. Total phenolic content and antioxidant capacity were positively associated with sorghum level. Protein digestibility was associated negatively with sorghum level and positively with barrel temperature. Extrudate density was associated positively with total moisture and negatively with barrel temperature and input rate. Sorghum in dry mix, final barrel zone temperature and total moisture in barrel were the three most significant independent variables influencing extrudate dependant variables