Selection of turning-on fluorogenic probe as protein-specific detector obtained via the 10BASEd-T

In order to obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherification (10BASEd−T). A remarkable turning-on probe which is excitable by widely applicable visible light was selected from the library

A World of Indirectness. Notes Toward a Study of Characterization in ‘The Tale of Genji’

Characters in the early eleventh-century ‘Tale of Genji’ are often represented indirectly, through others’ thoughts, speculations, and sensory impressions. Direct visual descriptions of a character’s physical appearance are relatively rare, but major plot developments result from scenes of kaimami (‘peeking through the crack’) when male characters catch a secret glimpse of women. Sensory impressions like sound, smell, and touch are sometimes as important as visual impressions in the courtly society of the Heian period when it was the custom for upper-class women to avoid showing themselves to men. This paper examines the importance of indirectness in the narrative’s representation of characters.Characters in the early eleventh-century ‘Tale of Genji’ are often represented indirectly, through others’ thoughts, speculations, and sensory impressions. Direct visual descriptions of a character’s physical appearance are relatively rare, but major plot developments result from scenes of kaimami (‘peeking through the crack’) when male characters catch a secret glimpse of women. Sensory impressions like sound, smell, and touch are sometimes as important as visual impressions in the courtly society of the Heian period when it was the custom for upper-class women to avoid showing themselves to men. This paper examines the importance of indirectness in the narrative’s representation of characters

Atmospheric neutrino flux at INO, South Pole and Pyh\"asalmi

We present the calculation of the atmospheric neutrino fluxes for the neutrino experiments proposed at INO, South Pole and Pyh\"asalmi. Neutrino fluxes have been obtained using ATMNC, a simulation code for cosmic ray in the atmosphere. Even using the same primary flux model and the interaction model, the calculated atmospheric neutrino fluxes are different for the different sites due to the geomagnetic field. The prediction of these fluxes in the present paper would be quite useful in the experimental analysis.Comment: 12Pages,9Fig

Importance of damage data from destructive earthquakes forseismic microzoning Damage distribution during the 1923 Kanto, Japan, earthquake

To verify the results of seismic microzoning and to improve techniques, the damage data of past destructive earthquakes is an important key reference. The damage data of the 1923 Kanto, Japan, earthquake in the epicentral region are collected and compiled to produce the most reliable and detailed damage map. The damage map is compared with the results from the existing damage assessment and is used to discuss revision of the site amplification evaluation

Superoxide dismutases enhance H2O2-induced DNA damage and alter its site specificity

AbstractSuperoxide dismutases (SODs) are involved in the protection of cells from oxygen toxicity. However, several papers have reported that the overexpression of CuZn-SOD causes oxidative damage to cells. We investigated a mechanism by which an excess of SODs accelerates oxidative stress. The presence of CuZn-SOD, Mn-SOD or Mn(II) enhanced the frequency of DNA damage induced by hydrogen peroxide (H2O2) and Cu(II), and altered the site specificity of the latter: H2O2 induced Cu(II)-dependent DNA damage with high frequency at the 5′-guanine of poly G sequences; when SODs were added, the frequency of cleavages at thymine and cytosine residues increased. SODs also enhanced the formation of 8-oxo-7,8-dihydro-2′-deoxyguanosine by H2O2 and Cu(II). We conclude that SODs may increase carcinogenic risks, e.g. of tumors in Down syndrome