The Cyrilka Cave-the longest crevice-type cave in Czechia: structural controls, genesis, and age

The Cyrilka Cave is the second longest pseudokarst cave and the longest crevice-type cave in Czechia. Developed within the headscarp area of a deep-seated landslide, the cave became a focus of scientific research in recent years when new passages were discovered. Structural analysis provided a general tectonic plan of the cave, as well as more detailed data on geometry and kinematics of the relaxed rock massif. The primary structure of NNE- to ENE-striking bedding is broken by a system of NNE-striking fissures interconnected by two continuous ENE-striking dextral fracture zones. Abundant signs of recent sinistral strike-slips within the rock massif represent a bold structural feature of the cave. Along with DEM imaging and a detailed survey of the cave, 2-D and 3-D ERT measurements completed an image of the main predispositions and revealed the internal structure of the slope deformation. These measures also detected unknown crevices above the existing headscarp, which indicate the retrograde evolution of the landslide. Methodologically, we used the 3-D electrical resistivity tomography in the incoherent sedimentary flysch rocks for the first time. Based on radiocarbon dating of the stalactite core, the minimum age of the cave is up to 19,900 +/- 280 cal BP, which is the oldest age detected in the area of the Outer Flysch Carpathians so far; we thoroughly discuss further indirect evidence indicating a probable Late Pleistocene age of the cave.Web of Science47339237

Observations and models for needle-tissue interactions

The asymmetry of a bevel-tip needle results in the needle naturally bending when it is inserted into soft tissue. In this study we present a mechanics-based model that calculates the deflection of the needle embedded in an elastic medium. Microscopic observations for several needle- gel interactions were used to characterize the interactions at the bevel tip and along the needle shaft. The model design was guided by microscopic observations of several needle- gel interactions. The energy-based model formulation incor- porates tissue-specific parameters such as rupture toughness, nonlinear material elasticity, and interaction stiffness, and needle geometric and material properties. Simulation results follow similar trends (deflection and radius of curvature) to those observed in macroscopic experimental studies of a robot- driven needle interacting with different kinds of gels. These results contribute to a mechanics-based model of robotic needle steering, extending previous work on kinematic models

Changes in growth of tropical forests: evaluating potential biases

Over the past century almost every ecosystem on Earth has come under the influence of changes in atmospheric composition and climate caused by human activity. Tropical forests are among the most productive and extensive ecosystems, and it has been hypothesized that both the dynamics and biomass of apparently undisturbed, old-growth tropical forests have been changing in response to atmospheric changes. Long-term forest sample plots are a critical tool in detecting and monitoring such changes, and our recent analysis of pan-tropical-forest plot data has suggested that the biomass of tropical forests has been increasing, providing a modest negative feedback on the rate of accumulation of atmospheric CO2. However, it has been argued that some of these old forest plot data sets have significant problems in interpretation because of the use of nonstandardized methodologies. In this paper we examine the extent to which potential field methodological errors may bias estimates of total biomass change by detailed examination of tree-by-tree records from up to 120 Neotropical plots to test predictions from theory. Potential positive biases on measurements of biomass change include a bias in site selection, tree deformities introduced by the measurement process, poor methodologies to deal with tree deformities or buttresses, and nonrecording of negative growth increments. We show that, while it is important to improve and standardize methodologies in current and future forest-plot work, any systematic errors introduced by currently identified biases in past studies are small and calculable. We conclude that most tropical-forest plot data are of useful quality, and that the evidence does still weigh conclusively in favor of a recent increase of biomass in old-growth tropical forests

Top effective operators at the ILC

We investigate the effect of top trilinear operators in t tbar production at the ILC. We find that the sensitivity to these operators largely surpasses the one achievable by the LHC either in neutral or charged current processes, allowing to probe new physics scales up to 4.5 TeV for a centre of mass energy of 500 GeV. We show how the use of beam polarisation and an eventual energy upgrade to 1 TeV allow to disentangle all effective operator contributions to the Ztt and gamma tt vertices.Comment: LaTeX 13 pages. Typos corrected. Final version in JHE

Demand responsive transport: A review of schemes in England and Wales

Local-authority-administered Demand Responsive Transport (DRT) schemes are increasingly prevalent in England and Wales, partly as a result of the growth in the availability of government funding. However, insufficient research has been undertaken into the nature of these schemes and their performance, making it difficult to predict their future role. In this respect, a survey was undertaken to collect data on the background, operation, and performance of DRT schemes in England and Wales. It found that DRT schemes are often designed in an attempt to tackle social problems caused by poor accessibility and that they took time to become established, to achieve their objectives, and to reach an acceptable performance in terms of subsidy level. The paper concludes that local-authority-led DRT schemes have a role to play, but that lessons learned from schemes currently in operation must be heeded by those contemplating new scheme development

A primordial origin for the atmospheric methane of Saturn's moon Titan

The origin of Titan's atmospheric methane is a key issue for understanding the origin of the Saturnian satellite system. It has been proposed that serpentinization reactions in Titan's interior could lead to the formation of the observed methane. Meanwhile, alternative scenarios suggest that methane was incorporated in Titan's planetesimals before its formation. Here, we point out that serpentinization reactions in Titan's interior are not able to reproduce the deuterium over hydrogen (D/H) ratio observed at present in methane in its atmosphere, and would require a maximum D/H ratio in Titan's water ice 30% lower than the value likely acquired by the satellite during its formation, based on Cassini observations at Enceladus. Alternatively, production of methane in Titan's interior via radiolytic reactions with water can be envisaged but the associated production rates remain uncertain. On the other hand, a mechanism that easily explains the presence of large amounts of methane trapped in Titan in a way consistent with its measured atmospheric D/H ratio is its direct capture in the satellite's planetesimals at the time of their formation in the solar nebula. In this case, the mass of methane trapped in Titan's interior can be up to 1,300 times the current mass of atmospheric methane.Comment: Accepted for publication in Icaru

The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin.

Trypanosoma cruzi glutathione-dependent peroxidase I (TcGPXI) can reduce fatty acid, phospholipid, and short chain organic hydroperoxides utilizing a novel redox cycle in which enzyme activity is linked to the reduction of trypanothione, a parasite-specific thiol, by glutathione. Here we show that TcGPXI activity can also be linked to trypanothione reduction by an alternative pathway involving the thioredoxin-like protein tryparedoxin. The presence of this new pathway was first detected using dialyzed soluble fractions of parasite extract. Tryparedoxin was identified as the intermediate molecule following purification, sequence analysis, antibody studies, and reconstitution of the redox cycle in vitro. The system can be readily saturated by trypanothione, the rate-limiting step being the interaction of trypanothione with the tryparedoxin. Both tryparedoxin and TcGPXI operate by a ping-pong mechanism. Overexpression of TcGPXI in transfected parasites confers increased resistance to exogenous hydroperoxides. TcGPXI contains a carboxyl-terminal tripeptide (ARI) that could act as a targeting signal for the glycosome, a kinetoplastid-specific organelle. Using immunofluorescence, tagged fluorescent proteins, and biochemical fractionation, we have demonstrated that TcGPXI is localized to both the glycosome and the cytosol. The ability of TcGPXI to use alternative electron donors may reflect their availability at the corresponding subcellular sites

Clathration of Volatiles in the Solar Nebula and Implications for the Origin of Titan's atmosphere

We describe a scenario of Titan's formation matching the constraints imposed by its current atmospheric composition. Assuming that the abundances of all elements, including oxygen, are solar in the outer nebula, we show that the icy planetesimals were agglomerated in the feeding zone of Saturn from a mixture of clathrates with multiple guest species, so-called stochiometric hydrates such as ammonia hydrate, and pure condensates. We also use a statistical thermodynamic approach to constrain the composition of multiple guest clathrates formed in the solar nebula. We then infer that krypton and xenon, that are expected to condense in the 20-30 K temperature range in the solar nebula, are trapped in clathrates at higher temperatures than 50 K. Once formed, these ices either were accreted by Saturn or remained embedded in its surrounding subnebula until they found their way into the regular satellites growing around Saturn. In order to explain the carbon monoxide and primordial argon deficiencies of Titan's atmosphere, we suggest that the satellite was formed from icy planetesimals initially produced in the solar nebula and that were partially devolatilized at a temperature not exceeding 50 K during their migration within Saturn's subnebula. The observed deficiencies of Titan's atmosphere in krypton and xenon could result from other processes that may have occurred both prior or after the completion of Titan. Thus, krypton and xenon may have been sequestrated in the form of XH3+ complexes in the solar nebula gas phase, causing the formation of noble gas-poor planetesimals ultimately accreted by Titan. Alternatively, krypton and xenon may have also been trapped efficiently in clathrates located on the satellite's surface or in its atmospheric haze.Comment: Accepted for publication in The Astrophysical Journa

RNA Viral Community in Human Feces: Prevalence of Plant Pathogenic Viruses

The human gut is known to be a reservoir of a wide variety of microbes, including viruses. Many RNA viruses are known to be associated with gastroenteritis; however, the enteric RNA viral community present in healthy humans has not been described. Here, we present a comparative metagenomic analysis of the RNA viruses found in three fecal samples from two healthy human individuals. For this study, uncultured viruses were concentrated by tangential flow filtration, and viral RNA was extracted and cloned into shotgun viral cDNA libraries for sequencing analysis. The vast majority of the 36,769 viral sequences obtained were similar to plant pathogenic RNA viruses. The most abundant fecal virus in this study was pepper mild mottle virus (PMMV), which was found in high concentrations—up to 10(9) virions per gram of dry weight fecal matter. PMMV was also detected in 12 (66.7%) of 18 fecal samples collected from healthy individuals on two continents, indicating that this plant virus is prevalent in the human population. A number of pepper-based foods tested positive for PMMV, suggesting dietary origins for this virus. Intriguingly, the fecal PMMV was infectious to host plants, suggesting that humans might act as a vehicle for the dissemination of certain plant viruses

Geodetic, teleseismic, and strong motion constraints on slip from recent southern Peru subduction zone earthquakes

We use seismic and geodetic data both jointly and separately to constrain coseismic slip from the 12 November 1996 M_w 7.7 and 23 June 2001 M_w 8.5 southern Peru subduction zone earthquakes, as well as two large aftershocks following the 2001 earthquake on 26 June and 7 July 2001. We use all available data in our inversions: GPS, interferometric synthetic aperture radar (InSAR) from the ERS-1, ERS-2, JERS, and RADARSAT-1 satellites, and seismic data from teleseismic and strong motion stations. Our two-dimensional slip models derived from only teleseismic body waves from South American subduction zone earthquakes with M_w > 7.5 do not reliably predict available geodetic data. In particular, we find significant differences in the distribution of slip for the 2001 earthquake from models that use only seismic (teleseismic and two strong motion stations) or geodetic (InSAR and GPS) data. The differences might be related to postseismic deformation or, more likely, the different sensitivities of the teleseismic and geodetic data to coseismic rupture properties. The earthquakes studied here follow the pattern of earthquake directivity along the coast of western South America, north of 5°S, earthquakes rupture to the north; south of about 12°S, directivity is southerly; and in between, earthquakes are bilateral. The predicted deformation at the Arequipa GPS station from the seismic-only slip model for the 7 July 2001 aftershock is not consistent with significant preseismic motion