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    62023 research outputs found

    Learning from Experience: Librarians as Research Partners

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    Given current discussions in the field about librarians’ engagement in research partnerships, this study examines the experiences of librarians at The Ohio State University, a doctoral university with very high research (R1), to better understand their preparation for the collaborations, the knowledge and skills they contributed, their roles on project teams, their motivation for joining teams, and the subsequent criteria they have adopted for determining participation on future projects. Interview and post-interview survey findings indicate librarians have developed their own strategies for determining their roles on research teams based on their individual strengths and that they have benefited from the experiences overall. This study also reveals inconsistencies in support for this form of engagement and makes recommendations for libraries related to scoping involvement and establishing criteria for participation of librarians on research teams.Publisher allows immediate open acces

    A Cutting-Edge Magnetic Immunocapture Method to Isolate Cell-Type-Specific Mitochondria from Complex Neural Tissue

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    Best Undergraduate Poster (Discovery Category) - OSU CBI Research Day 2025Alterations in neuronal and glial metabolism contribute to numerous neurodegenerative diseases, and the crosstalk between these two cell types (i.e., axoglial metabolic coupling) is at the center of extensive investigation. Metabolic alterations frequently culminate in mitochondrial dysfunction, but so far it has proven challenging to obtain cell-type-specific metabolic data from neuronal or glial mitochondria in mouse models of injury or disease. Magnetic immunocapture of genetically tagged mitochondria has emerged as a powerful strategy, yet existing methods are either incompatible with sensitive downstream multi omic workflows or not yet tested in complex tissues with highly heterogeneous cell populations. Here, I refined the “MITO-Tag” approach, which leverages a Cre dependent 3×HA EGFP OMP25 epitope tag localized to the outer mitochondrial membrane. Following enzymatic and mechanical dissociation, mitochondria were rapidly immunopurified from cortical neurons, oligodendrocyte lineage cells, and—accomplished here for the first time—peripheral nerve axons using anti-HA magnetic beads in liquid chromatography-tandem mass spectrometry (LC-MS/MS)-compatible KPBS buffer. This method yields specific and structurally intact mitochondria, as confirmed by live-organelle imaging and Western blotting for compartment-specific markers (COXIV, VDAC, citrate synthase), with minimal contamination from other organelles. Proteomic analysis of brain-derived immunoprecipitates (IPs) revealed mitochondrial enrichment comparable to existing magnetic immunopurification workflows. By enabling multi-omic mitochondrial profiling from moderate-abundance cell types within complex tissues, this method provides a versatile tool for investigating mitochondrial involvement in neurological disease and injury in vivo. Notably, isolation from peripheral nerve axons now offers the ability to characterize Wallerian degeneration and axoglial metabolic coupling mechanisms at an unprecedented resolution.NIH R01NS123450-01NIH R01NS111024-02OSU College of Engineering Thesis Research Grant2025 CAN-ACN Travel GrantNo embargoAcademic Major: Biomedical Engineerin

    2025 Rowing Record Book

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    2025 Women's Volleyball Record Book

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    Prediction of Optimal Parameters for Wire-Arc DED Welding Using a Multilayer Perceptron Trained on Synthetic Data Generated by a Generative Adversarial Network

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    The global demand for 3D printing technologies has grown significantly, influencing various industries and extending into the welding sector through additive manufacturing (AM). In welding, AM involves constructing metal parts layer by layer using wire or powder feedstock. This method offers advantages such as material efficiency and design flexibility. However, bead overlapping between layers often results in defects like surface irregularities and weakened structural integrity. This paper presents an automated approach to optimize welding parameters using a multi-layer perceptron (MLP) neural network. Traditional methods for selecting parameters such as current, voltage, and wire feed rate are time-consuming and resource-intensive. To address limited data availability, synthetic data were generated using a generative adversarial network (GAN). By training the MLP on this expanded dataset, the system predicts parameters that improve bead quality and inter-layer bonding. This AI-driven approach reduces the need for extensive physical experimentation, minimizes material waste, and shortens development cycles.No embargoAcademic Major: Computer and Information Scienc

    Other Lovings: An AfroAsian American Theory of Life

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    Introduction: Other lovings and racial ontology -- Audre Lorde and affectable flesh -- David Henry Hwang and Asian American love-being -- The Amiri Baraka of surplus love -- Jeremy Lin, G Yamazawa, Lyricks: an AfroAsian commons -- Gayl Jones and somatic wisdom -- Adrian Tomine and the love of the Asian American object -- Theodor Adorno, Eve Sedgwick, and the Kyoto School's optimism -- Conclusion: bell hooks's and Charles Yu's love-being in practice.Item embargoed for five year

    Supporting Evidence for Competencies

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    Evaluating Mouthguard Performance: A Novel Test and Maxilla Strain Analysis

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    Oral and dental injuries are common in high-contact sports, yet many players forgo mouthguards due to discomfort and poor fit. Methods for mouthguard testing exist; however, developing a testing environment that accurately reproduces real-world impact scenarios is inherently difficult due to the limitations of replicating such scenarios in a laboratory setting. Prior studies have laid the groundwork for mouthguard testing; however, they also reveal notable limitations in their testing methodology. With a lack of a universally standardized testing method and the gaps observed in previous research, there is a clear need for a novel testing design. This forms the basis for the objectives of this study, which includes developing a realistic and consistent testing methodology that directly addresses the current challenges in mouthguard evaluation and using the proposed methodology to evaluate the mechanical performance and protective effectiveness of leading commercial mouthguards. The testing method of this study is a drop-tower apparatus that features a vertically positioned, 3D-printed stainless steel of the student’s maxilla to ensure consistent mouthguard fitting and results. A modified rubber puck, positioned above the central incisors, served as the impactor. Impact stress on the jaw was quantified with a 5,000 pound-force load cell and analyzed via oscilloscope. Digital Image Correlation, a technique for tracking deformation on a targeted surface, was utilized to measure strain. The flat surface of the horseshoe-shaped jaw was marked with a speckle pattern, with high-speed camera footage of each test processed in VIC-2D. MATLAB was used to analyze average strain and strain under impact energies of 21, 48, and 84-joules. The testing apparatus was successful in determining the performance of a range of commercial mouthguards. Overall, the results indicate that the methodology developed in this study offers strong potential as a standardized framework for future mouthguard evaluation.No embargoAcademic Major: Mechanical Engineerin

    Prohexadione-calcium Foliar Sprays for Growth Regulation in Herbaceous Perennial Ornamental Grass Taxa

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    Excessive vegetative growth of ornamental grasses presents several production challenges for commercial greenhouse and nursery growers. Prohexadione-calcium, a plant growth regulator (PGR), has been used to reduce growth in a variety of fruit and grain crops. The foliar influence of prohexadione-calcium on five popular and commercially available ornamental grasses was evaluated in this study. Calamagrostis acutiflora ‘Karl Foerster’, Miscanthus sinensis ‘Huron Sunrise’, Panicum virgatum ‘Red Flame’, Pennisetum alopecuroides ‘Hameln’, and Schizachyrium scoparium ‘Twilight Zone’ were sprayed with 0, 500, 1000, 1500, 2000, 2500, 3000, or 5000 mg·L–1 prohexadione-calcium in a completely randomized block design. Each species received one spray application and were evaluated 2, 4, and 6 weeks after application to determine plant height and diameter. After 6 weeks, the experiment was terminated, and plants were excised at the substrate level to determine shoot dry weight. Prohexadione-calcium significantly (P ≤ 0.05) reduced plant height, plant diameter, and shoot dry weight of Miscanthus ‘Huron Sunrise’, compared to the control. Plant height and plant diameter of Schizachyrium ‘Twilight Zone’ was only affected 6 weeks after application and reduced shoot dry weight. While prohexadione-calcium applications on Miscanthus ‘Huron Sunrise’, Panicum ‘Red Flame’ and Pennisetum ‘Hameln’ had minimal to no effect on plant height or plant diameter but varying significance on shoot dry weight. Data from this study suggests that foliar application of prohexadione-calcium on ornamental grasses shows promise but is species- and concentration specific.The Ohio State Floriculture and Landscape Horticulture Alumni Association Fellowship Endowment FundFine Americas, IncSun Gro HorticultureJ.R. Peters, Inc.Walters Gardens, Inc.No embargoAcademic Major: Sustainable Plant System

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