Analysis of the hard-tissue menton shape variation in adult South Africans using cone-beam computed tomography (CBCT) scans

BACKGROUND/OBJECTIVE : In forensic anthropology, the biological profile is based on human variation and can help in the process of personal identification. In order to better understand shape variation of the mental region, this study analyzed the influence of population affinity and sex on the menton in adult black and white South Africans, using geometric morphometric methods (GMM). MATERIALS AND METHODS : We used cone-beam computed tomography (CBCT) scans of 291 adult dental patients with dentition patterns up to Eichner Index B3, retrospectively collected from the Oral and Dental Hospital, University of Pretoria. We placed eleven standard craniometric landmarks on the menton, mandible, and maxilla of threedimensional (3D) reconstructions by automatic landmarking and analyzed them by applying GMM. In addition, a subtle shape matrix of seven landmarks was created for a focused analysis of the menton only. Finally, we tested the reproducibility of the landmarks placement with a dispersion analysis. RESULTS : The landmarks used in this study were reproducible, with an overall dispersion of less than 1 mm. Population affinity significantly influenced menton shape, with P-values = 0.001 in the complete sample and within the sex groups. Differences between sexes for these seven landmarks were also statistically significant (Pvalues between 0.001 to 0.003) in the complete sample, but not within population groups in isolation. The accuracy for estimation of population affinity by discriminant function analysis was 86.9%. CONCLUSION : The use of automatic landmarking improved landmark reproducibility. Population affinity and sexual dimorphism significantly influenced menton shape. However, shape analysis, including all eleven landmarks, was not significantly influenced by sex. This study supports further research focusing on the facial approximations for forensic identification in South Africa.UP Postgraduate Bursary and the Bakeng se Afrika funding.https://www.sciencedirect.com/journal/forensic-imagingam2024AnatomySDG-03:Good heatlh and well-bein

Repeatability of a morphoscopic sex estimation technique for the mental eminence on micro-focus X-ray computed tomography models

Objectives Walker's sex estimation method is based on five morphoscopic features of the human skull. This study aimed at testing the repeatability of one of the five traits, the mental eminence, visually on three-dimensional (3D) models, compared to the traditional, tactile scoring approach on bone. Materials and methods The sample comprised 105 mandibles from the Pretoria Bone Collection and their respective virtual 3D models, obtained from micro-focus X-ray computed tomography (micro-XCT) scans. Four observers independently scored the bones first, followed by the virtual 3D modality. Intra- and interobserver errors (interOE and intraOE) were performed with Fleiss’ and Cohen's Kappa, respectively. We calculated the intermodality agreement per observer with Wilcoxon Signed-Rank tests. Results The intraOE was moderate on bone (κ=0.448) and substantial on 3D (κ=0.799), while the Fleiss’ Kappa test for the interOE resulted in slight agreement both on bone (κ=0.163) and 3D (κ=0.169) irrespective of level of experience. All Wilcoxon Signed-Rank test P-values were significant. Discussion and conclusion The application of the morphoscopic sex estimation for the mental eminence to micro-XCTs could be a matter of personal affinity as the level of experience did not play a role in the results. The expression of the mental eminence trait being population-specific, the individual's population affinity should be considered when sex is estimated in South African skeletons. It remains unclear whether the slight agreement between observers is due to the unreliability of the trait for sex estimation or whether the modalities are not easily interchangeable

Repeatability of a morphoscopic sex estimation technique for the mental eminence on micro-focus X-ray computed tomography models

OBJECTIVES : Walker's sex estimation method is based on five morphoscopic features of the human skull. This study aimed at testing the repeatability of one of the five traits, the mental eminence, visually on three-dimensional (3D) models, compared to the traditional, tactile scoring approach on bone. MATERIALS AND METHODS : The sample comprised 105 mandibles from the Pretoria Bone Collection and their respective virtual 3D models, obtained from micro-focus X-ray computed tomography (micro-XCT) scans. Four observers independently scored the bones first, followed by the virtual 3D modality. Intra- and interobserver errors (interOE and intraOE) were performed with Fleiss’ and Cohen's Kappa, respectively. We calculated the intermodality agreement per observer with Wilcoxon Signed-Rank tests. RESULTS : The intraOE was moderate on bone (κ=0.448) and substantial on 3D (κ=0.799), while the Fleiss’ Kappa test for the interOE resulted in slight agreement both on bone (κ=0.163) and 3D (κ=0.169) irrespective of level of experience. All Wilcoxon Signed-Rank test P-values were significant. DISCUSSION AND CONCLUSION : The application of the morphoscopic sex estimation for the mental eminence to micro-XCTs could be a matter of personal affinity as the level of experience did not play a role in the results. The expression of the mental eminence trait being population-specific, the individual's population affinity should be considered when sex is estimated in South African skeletons. It remains unclear whether the slight agreement between observers is due to the unreliability of the trait for sex estimation or whether the modalities are not easily interchangeable.UP Postgraduate Bursary, AESOP Erasmus Mundus program, the University of Pretoria, the National Research Foundation of South Africa, the Bakeng se Afrika funding and Erasmus+ KA2 Capacity Building in Higher Education.https://www.sciencedirect.com/journal/forensic-imaginghj2022Anatom

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements