100 research outputs found
Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation
Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1
Retinal S-antigen Th1 cell epitope mapping in patients with Behcet's disease
Background - Retinal S-antigen (S-Ag) is a most characterized autoantigen of autoimmune uveitis. The recognized immunodominant epitope of human S-Ag in patients with uveitis has not been identified. In this study, we selected certain patients with active uveitis to map the Th1 cell epitope spectrum of human S-Ag in Behcet's disease(BD). Methods - Blood samples were taken from eight active BD patients who showed an immune response to 40 mixed overlapping peptides spanning the entire sequence of human S-Ag. Peripheral blood mononuclear cells were isolated and stimulated with single S-Ag peptide at 5 mu g/ml or 20 mu g/ml. Single-cell immune responses were measured by IFN-gamma ELIspot assay. Results - BD patients heterogeneously responded to the S-Ag peptides at two concentrations. In general, the responses to 5 mu g/ml peptides were slightly stronger than those to 20 mu g/ml peptides, while the maximum SFC frequency to single peptide at the two concentrations was similar. Several peptides including P31, P35 and P40 induced a prominent response, with the frequency of S-Ag specific cells being about 0.007%. Significant reactivity pattern shift was noted in patients with different disease courses. Conclusions - Certain active BD patients have S-Ag specific Th1 cells with a low frequency. The S-Ag epitope specificity between patients is highly heterogeneous, and varies with the uveitis cours
Light-Front Approach for Pentaquark Strong Decays
Assuming the two diquark structure for the pentaquark state as advocated in
the Jaffe-Wilczek model, we study the strong decays of light and heavy
parity-even pentaquark states using the light-front quark model in conjunction
with the spectator approximation. The narrowness of the Theta width is ascribed
to the p-wave configuration of the diquark pair. Taking the Theta width as a
benchmark, we estimate the rates of the strong decays Xi_{3/2}-- to Xi- pi-,
Sigma- K-, Sigma_{5c}0 to D_s- p, D_{s0}*- p and Xi_{5c}0 to D_s- Sigma+,
D_{s0}^{*-} Sigma+ with Sigma_{5c} Xi_{5c} being antisextet charmed pentaquarks
and D_{s0}* a scalar strange charmed meson. The ratio of Gamma(P_c to Baryon
D_{s0}*)/Gamma(P_c to Baryon D_s) is very useful for verifying the parity of
the antisextet charmed pentaquark P_c. It is expected to be of order unity for
an even parity P_c and much less than one for an odd parity pentaquark.Comment: 24 pages, 2 figure
Nuclear Structure Functions in the Large x Large Q^2 Kinematic Region in Neutrino Deep Inelastic Scattering
Data from the CCFR E770 Neutrino Deep Inelastic Scattering (DIS) experiment
at Fermilab contain events with large Bjorken x (x>0.7) and high momentum
transfer (Q^2>50 (GeV/c)^2). A comparison of the data with a model based on no
nuclear effects at large x, shows a significant excess of events in the data.
Addition of Fermi gas motion of the nucleons in the nucleus to the model does
not explain the excess. Adding a higher momentum tail due to the formation of
``quasi-deuterons'' makes some improvement. An exponentially falling F_2
\propto e^-s(x-x_0) at large x, predicted by ``multi-quark clusters'' and
``few-nucleon correlations'', can describe the data. A value of s=8.3 \pm
0.7(stat.)\pm 0.7(sys.) yields the best agreement with the data.Comment: 4 pages, 4 figures, 1 table. Sibmitted to PR
Transverse sphericity of primary charged particles in minimum bias proton-proton collisions at , 2.76 and 7 TeV
Measurements of the sphericity of primary charged particles in minimum bias
proton--proton collisions at , 2.76 and 7 TeV with the ALICE
detector at the LHC are presented. The observable is linearized to be collinear
safe and is measured in the plane perpendicular to the beam direction using
primary charged tracks with GeV/c in . The
mean sphericity as a function of the charged particle multiplicity at
mid-rapidity () is reported for events with different
scales ("soft" and "hard") defined by the transverse momentum of the leading
particle. In addition, the mean charged particle transverse momentum versus
multiplicity is presented for the different event classes, and the sphericity
distributions in bins of multiplicity are presented. The data are compared with
calculations of standard Monte Carlo event generators. The transverse
sphericity is found to grow with multiplicity at all collision energies, with a
steeper rise at low , whereas the event generators show the
opposite tendency. The combined study of the sphericity and the mean with multiplicity indicates that most of the tested event generators
produce events with higher multiplicity by generating more back-to-back jets
resulting in decreased sphericity (and isotropy). The PYTHIA6 generator with
tune PERUGIA-2011 exhibits a noticeable improvement in describing the data,
compared to the other tested generators.Comment: 21 pages, 9 captioned figures, 3 tables, authors from page 16,
published version, figures from
http://aliceinfo.cern.ch/ArtSubmission/node/308
Limits on and Oscillations from a Precision Measurement of Neutrino-Nucleon Neutral Current Interactions
We present limits on and
oscillations based on a study of
inclusive interactions performed using the CCFR massive coarse grained
detector in the FNAL Tevatron Quadrupole Triplet neutrino beam. The sensitivity
to oscillations is from the difference in the longitudinal energy deposition
pattern of versus or charged current
interactions. The energies ranged from 30 to 500 GeV with a mean of
140 GeV. The minimum and maximum flight lengths are 0.9 km and 1.4 km
respectively. For oscillations, the lowest 90% confidence
upper limit in of is obtained at ~eV. This result is the most stringent limit to date for
eV. For oscillations, the lowest 90%
confidence upper limit in of is obtained at
~eV. This result is the most stringent limit to date for
eV, and also excludes at 90% confidence much of the
high region favored by the recent LSND observation.Comment: Revised version contains limit on oscillations as
well as limit on oscillations found in original. 15
pages, ReVTeX, 3 figures in uuencoded file, submitted to PR
CaracterĂsticas sensoriais, fĂsicas e quĂmicas e aceitação de arroz irrigado ou de terras altas
Resumo:O objetivo deste trabalho foi caracterizar quĂmica, fĂsica e sensorialmente trĂȘs genĂłtipos de arroz polido de cultivo irrigado e cinco de terras altas. Determinaram-se: a composição centesimal, o amido, a amilose, a temperatura de gelatinização (Tg), a absorção de ĂĄgua ao cozimento, a cor, a textura instrumental, o perfil sensorial e a aceitação de atributos. A composição centesimal e o teor de amido variaram entre os genĂłtipos. O 'Moti' e a linhagem N2583 foram classificados como cerosos, 'DouradĂŁo' apresentou amilose muito baixa, e os demais genĂłtipos, amilose baixa. 'Irga 417', 'Moti' e AB101002 apresentaram Tg baixa; 'DouradĂŁo', alta; e os demais, intermediĂĄria. O 'Moti' e a linhagem N2583 crus apresentaram os maiores valores de L*; no entanto, apĂłs o cozimento, esses valores diminuĂram e foram idĂȘnticos em todos os genĂłtipos. 'Arroz-da-terra' apresentou maior a*, 'BRS Primavera' e N2583 apresentaram maior b*; e apĂłs o cozimento, houve redução desses valores. Na anĂĄlise sensorial, destacaram-se 'AN CambarĂĄ', por pontos escuros e formato alongado; AB101002, 'Irga 417' e 'BRS Primavera', pela firmeza, cor branca e grĂŁos soltos, que foram bem aceitos; e o 'Moti', por ser pegajoso, macio e com brilho, foi o menos aceito. Os atributos sensoriais do arroz sĂŁo mais associados a caracterĂsticas quĂmicas, intrĂnsecas ao genĂłtipo, do que Ă atribuição de plantio a terras altas ou irrigadas
Measurement of prompt hadron production ratios in collisions at 0.9 and 7 TeV
The charged-particle production ratios , , ,
, and are measured with the LHCb detector using of collisions delivered by the LHC at TeV and
at TeV. The measurements are performed as a
function of transverse momentum and pseudorapidity . The
production ratios are compared to the predictions of several Monte Carlo
generator settings, none of which are able to describe adequately all
observables. The ratio is also considered as a function of rapidity
loss, , and is used to constrain models of
baryon transport.Comment: Incorrect entries in Table 2 corrected. No consequences for rest of
pape
Measurement of the forward energy flow in pp collisions at √<span style="text-decoration:overline">s</span>=7 TeV
The energy flow created in pp collisions at sâ=7 TeV is studied within the pseudorapidity range 1.9<η<4.9 with data collected by the LHCb experiment. The measurements are performed for inclusive minimum-bias interactions, hard scattering processes and events with an enhanced or suppressed diffractive contribution. The results are compared to predictions given by Pythia-based and cosmic-ray event generators, which provide different models of soft hadronic interactions
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